SA Z326K Eng V1.14 PDF
SA Z326K Eng V1.14 PDF
SA Z326K Eng V1.14 PDF
for
Universal
Centrifuge
Z 326 K
7 SERVICE INSTRUCTIONS
8 TROUBLE SHOOTING
9 MAINTENANCE
10 CIRCUIT DIAGRAMS
Rotor-Number
Level 0
Level 1
Level 2
Level 3
Level 4
Level 5
Level 6
Level 7
Level 8
Level 9
221.12 V03 1:35 0:48 0:33 0:25 0:21 0:18 0:16 0:15 0:15 0:15
220.72 V06 1:51 0:52 0:36 0:28 0:23 0:20 0:18 0:15 0:14 0:14
221.16 V03 2:39 1:20 0:53 0:41 0:33 0:29 0:24 0:22 0:20 0:18
221.19 V02 1:57 1:01 0:40 0:31 0:25 0:22 0:20 0:18 0:17 0:16
221.54 V02 1:27 0:44 0:29 0:22 0:18 0:15 0:14 0:12 0:12 0:12
221.55 V02 1:29 0:43 0:29 0:22 0:18 0:15 0:13 0:12 0:12 0:12
220.78 V05 8:15 4:10 2:43 2:04 1:40 1:23 1:13 1:07 1:02 1:01
221.18 V02 7:00 3:23 2:16 1:41 1:27 1:14 1:00 0:53 0:53 0:55
221.20 V02 5:09 2:39 1:45 1:20 1:13 1:12 1:16 1:17 1:18 1:17
221.22 V02 6:01 2:58 1:59 1:29 1:12 1:01 0:58 0:46 0:42 0:42
221.28 V02 6:08 2:57 1:59 1:29 1:12 1:01 0:52 0:47 0:47 0:47
221.17 V03 3:13 1:37 1:05 0:49 0:39 0:34 0:30 0:27 0:27 0:27
220.87 V09 3:29 1:44 1:11 0:53 0:43 0:36 0:31 0:29 0:26 0:23
221.87 V10 3:30 1:45 1:10 0:59 0:43 0:36 0:31 0:28 0:25 0:24
221.23 V02 3:55 1:57 1:18 0:59 0:51 0:43 0:35 0:32 0:28 0:26
220.88 V07 2:37 1:18 0:53 0:40 0:33 0:28 0:24 0:22 0:20 0:18
220.92 V06 2:18 1:11 0:49 0:36 0:30 0:25 0:22 0:20 0:18 0:16
221.38 V01 2:08 1:05 0:45 0:34 0:28 0:23 0:21 0:19 0:16 0:15
Rotor-Number
Level 0
Level 1
Level 2
Level 3
Level 4
Level 5
Level 6
Level 7
Level 8
Level 9
221.12 V03 3:18 0:45 0:34 0:28 0:25 0:22 0:19 0:18 0:18 0:16
220.72 V06 3:55 0:54 0:38 0:29 0:25 0:22 0:20 0:18 0:15 0:15
221.16 V03 7:27 0:49 0:34 0:26 0:23 0:19 0:18 0:17 0:16 0:16
221.19 V02 9:00 0:25 0:18 0:16 0:14 0:13 0:12 0:13 0:12 0:13
221.54 V02 6:33 0:32 0:22 0:18 0:16 0:15 0:12 0:11 0:11 0:11
221.55 V02 7:42 0:32 0:22 0:18 0:16 0:15 0:12 0:11 0:11 0:11
220.78 V05 23:27 3:52 2:32 1:55 1:33 1:17 1:06 0:58 0:52 0:46
221.18 V02 23:33 2:35 1:42 1:18 1:04 0:53 0:47 0:42 0:38 0:35
221.20 V02 17:22 2:03 1:24 1:04 0:53 0:47 0:41 0:39 0:36 0:36
221.22 V02 12:53 2:01 1:26 1:02 0:51 0:43 0:38 0:34 0:31 0:28
221.28 V02 14:35 2:02 1:22 1:03 0:51 0:44 0:38 0:35 0:31 0:29
221.17 V03 7:46 1:53 1:16 0:58 0:48 0:41 0:37 0:34 0:30 0:28
220.87 V09 5:11 1:12 0:49 0:37 0:30 0:26 0:22 0:20 0:18 0:16
221.87 V10 5:09 1:12 0:48 0:36 0:30 0:25 0:22 0:20 0:18 0:16
221.23 V02 5:02 1:32 1:02 0:47 0:38 0:32 0:28 0:24 0:23 0:20
220.88 V07 4:15 0:53 0:36 0:28 0:24 0:20 0:17 0:15 0:13 0:12
220.92 V06 3:46 0:54 0:37 0:29 0:24 0:21 0:18 0:16 0:14 0:13
221.38 V01 2:41 0:54 0:39 0:30 0:21 0:20 0:17 0:15 0:14 0:13
List of rotors which cannot be adjusted, but which are set by the work of using motion detectors.
List of rotors which have to be set by the imbalance switch. See chapter 7.2.9 and 7.4.13.
The acceleration and deceleration times may have slight fluctuations that depend on the unit and are
therefore guidelines only.
Please follow below mentioned safety instructions for any kind of service actions:
• The capacitor of the frequency converter can be under voltage even when the unit is switched off.
• Do not leave units unsupervised, when parts of the housing have been removed and the unit is still
connected to the main power supply.
• Do not bypass the lid’s safety contacts and never work with the unit’s lid open.
• The VDE regulations are valid for all electrical work that has to be done.
The power board is serving the low voltage supply of the centrifuge control system. The power board is
electrically isolated and has a dielectric strength of DC 2.2 kV.
The control board consists of one plate. The board can only be exchanged completely. If there is a
defect you have to exchange the complete board.
All signal lines lead to the control board.
The PCB controls the entire centrifuge.
The control panel consists of one LCD-display, one incremental shaft encoder, the upper front housing
and the foil keyboard. These parts can be exchanged separate. The incremental shaft encoder regulates
all parameters which are adjustable by pressing a key and which are indicated in the LCD-display. In the
speed display of the control panel the error message is indicated if there occur any troubles
The frequency converter generates the drive signals for the asynchronous motor.
The converter leads the current generated by the motor during deceleration to a heating resistor to
reduce the current.
The frequency converter is connected with the power board by a serial interface.
There is a green LED on the converter which flashes when the frequency converter is working correctly.
A defect of the converter will be indicated in the TIME display as an error no. (See chapter 8).
The centrifuge will recognize the inputted rotor through the rotor identification transponder and the aerial
belonging to it right after the insert automatically. As soon as the lid gets closed the rotor number will be
indicated in the speed display.
(See photo in chapter 7.2.3)
Aerial
Transponder
If the pre-selected rpm is higher as the permitted rpm of the rotor, the speed indication in the display will
be regulated to the max. speed permitted for this rotor.
The regulation will accelerate the rotor only to the permitted rpm of the rotor.
The actual speed is extracted by a hall-effect-sensor placed on the lower side of the motor.
There is a “preset/actual“ comparison in the control board. Thereupon a new “preset-signal” is given by
the control board to the converter.
(See also photo of chapter 7.2.6.)
The micro switch in the mechanism of the lid lock controls the correct closing of the centrifuge lid.
The switch may never be bypassed.
The lid lock is unlatched by an electro motor, receiving its signal from the control board.
The actual state is indicated in the display.
A micro switch, controls for certain rotors, the displacement of the motor and shut off if there is too much
imbalance. The micro switch should be adjusted according to the instruction of the company. (See
chapter 7.4.13. See also list 6.3)
Chapter 7.2.6
Chapter 7.2.9
Chapter 7.2.8
Break Resistance
on the back side Signal Transmitter
Chapter 7.2.4
Chapter 7.2.2
Chapter 7.2.1
The operation menu helps the service personnel to locate defects. It is divided in two parts, which are
graded in several areas again from chapter 7.3.1 to 7.3.2.3.
Part 1 is accessible for the USER and different points can be read respectively settled.This is also
described in the instruction manual.
Part 2 is for internal use respectively for trained service personnel outdoors Hermle LABORTECHNIK
only. The control board can be adjusted to different units. Here the different parameters are determined
on the respective unit after an exchange of the control board.
• Up to software version 1.26 press the keys time and prog hold them and at the same time switch on
the unit. From software version 1.27 please use the keys time and lid/open. As you can’t know
beforehand which software is installed in the unit, you have to try which combination will start operation
menu.
or
• After the indication displays flash on, led go of the keys time and prog, resp. time and lid/open.
In the display “rpm/rcf“ the stored type of the centrifuge (i.e. 216) and below a letter (i.e. c) is indicated
for about 2 seconds. The letter stands for the different versions of the units which are currently stored
in the control board:
c for cool
h for heat
no for nothing
Now it follows a display test for about 5 seconds as shown here under.
Now in the display “accel/decel“ the word “service“ will flash. Only as long as this word is flashing, you
can scroll through the further submenus with the potentiometer.
You have to repeat this step constantly to enter the different submenus.
• Turn the potentiometer until the special digit “service A“ appears in the display „acc/dec”.
Here you can read off the duty cycle of the unit.
• Turn the potentiometer until the special digit “service H“ appears in the display “acc/dec”.
Here you can read off the operating hours of the motor.
• Turn the potentiometer until the special digit “service h“ appears in the display “acc/dec”.
In the display “rpm/rcf“ the running time of the motor is indicated now.
• Turn the potentiometer until the special digit ”service S“ appears in the display „acc/dec”.
Here you can read off the software status of the frequency converter.
• Turn the potentiometer until the special digit „service r“ appears in the display „acc/dec”.
In the display „rpm/rcf“ the actual software status of the frequency converter is indicated now.
• Turn the potentiometer until the special digit “service E“ appears in the display “acc/dec”.
• By pressing the key rpm/rcf the display “rpm/rcf“ is activated and you can scroll through the
error list with the potentiometer.
• Now the last 99 occurred error messages are indicated in the display “rpm/rcf“. Whereas the
first two digits describe the place number and the last two digits do describe the occurred
error.
Here you can change the temperature unit from degree Celsius (°C) to degree Fahrenheit (°F).
• Turn the potentiometer until the special digit “service c“ appears in the display “acc/dec”.
• By pressing the key rpm/rcf the display “rpm/rcf“ is now activated and you can setup the
respective temperature unit with the potentiometer.
Attention: After all points have been reviewed and/or set up, you have to press shortly the key
start to store all parameters. Otherwise the setups were all for nothing. If the storage has been
successful in the display “rpm/rcf“ the value of the imbalance sensor and the word “StorE“ are
indicated.
Here you can switch on resp. switch off the signal generator (audible sound).
• Turn the potentiometer until the special digit “service L“ appears in the display “acc/dec”.
• By pressing the key rpm/rcf the display „rpm/rcf“ is now activated and you can setup the
respective option with the potentiometer.
• Now the audible sound is activated resp. not activated by the end of the run or when an error
message will occur.
Attention: In case that the signal generator is switch off, the keypad tone and the audible error
message will also be suppressed.
The keypad tone has to be switched on again after the reactivation of the signal generator.
However the audible error message is automatically activated if the signal generator has been
reactivated.
Attention: After all points have been reviewed and/or set up, you have to press shortly the key
start to store all parameters. Otherwise the setups were all for nothing. If the storage has been
successful in the display “rpm/rcf“ the value of the imbalance sensor and the word “StorE“ are
indicated.
Here you can switch on resp. switch off the keypad tone.
• Turn the potentiometer until the special digit “service b“ appears in the display “acc/dec”.
• By pressing the key rpm/rcf the display “rpm/rcf“ is now activated and you can setup the
respective option with the potentiometer.
Attention: In case that the signal generator is switched off, the keypad tone and the audible
error message will also be suppressed.
The keypad tone has to be switched on again after the reactivation of the signal generator.
However the audible error message is automatically activated if the signal generator has been
reactivated.
Here you can setup the volume of the loudspeaker in case the signal generator is activated.
• Turn the potentiometer until the special digit “service u“ appears in the display ”acc/dec”.
• By pressing the key rpm/rcf the display “rpm/rcf“ is now activated and you can setup the
respective option with the potentiometer.
• At the end of the run you can now hear the audible sound in the setup volume whereas 0 is
the most quiet setup and 9 the loudest one.
Attention: After all points have been reviewed and/or set up, you have to press shortly the key
start to store all parameters. Otherwise the setups were all for nothing. If the storage has been
successful in the display “rpm/rcf“ the value of the imbalance sensor and the word “StorE“ are
indicated.
Here you can setup the melody at the end of the run in case the signal generator is activated.
• Turn the potentiometer until the special digit “service G“ appears in the display “acc/dec”.
• By pressing the key rpm/rcf the display “rpm/rcf“ is now activated and you can setup the
respective option with the potentiometer.
• Now the setup melody will be played by the end of the run, whereas you can choose between
0 and 9 setups (anyway 10 melodies).
Attention: After all points have been reviewed and/or set up, you have to press shortly the key
start to store all parameters. Otherwise the setups were all for nothing. If the storage has been
successful in the display “rpm/rcf“ the value of the imbalance sensor and the word “StorE“ are
indicated.
7.3.1.12 Submenu Check and calibration of the imbalance sensor (Movement sensor):
• Turn the potentiometer until the special digit “service F“ appears in the display “acc/dec”.
Non-calibrated indication
• If the word „Error“ appears in the display, the imbalance sensor is defective and you have to
replace the whole rotary encoder board (mounted on the lower side of the motor).
Attention: In case you have to replace the motor or the rotary encoder board for any reason, a
new calibration is required urgently. The mechanical imbalance switch (micro switch) has to be
re-adjusted, too. (see chapter 7.4.13)
Attention: After all points have been reviewed and/or set up, you have to press shortly the key
start to store all parameters. Otherwise the setups were all for nothing. If the storage has been
successful in the display “rpm/rcf“ the value of the imbalance sensor and the word “StorE“ are
indicated.
Here you can read off the imbalance value of the rotor transponder.
• Turn the potentiometer until the special digit “service Y“ appears in the display “acc/dec“.
• In the bottom line of the display “rpm/rcf“ the real imbalance value is indicated. In the top line
the rotor number is indicated (in this case 221.17 V0?).
The keyboard test is used for checking the correct function of the foil keyboard.
• Turn the potentiometer until the special digit “service P“ appears in the display “acc/dec“.
• By pressing each key the word “PrESS“ switches from the bottom to the top line in the display
“rpm/rcf“. The key accel/decel excluded.
Here you can read off the revision number of the control board. From the revision number 2 and
the software version 1.33 on or higher, you have the possibility to check the function of the
imbalance sensor.
• Turn the potentiometer until the special digit “service d“ appears in the display “acc/dec“.
To get access to the second level of the service menu you have to press the key stop for longer than
one second. Now you are asked for a code in the display “rpm/rcf“ – adjust the code “9876“ with the
potentiometer and press once again the key stop.
Here you can adjust the different centrifuge types that are stored on the control board.
• Turn the potentiometer until the special digit “service t“ appears in the display “acc/dec“.
• Examples:
• By pressing the key rpm/rcf the display “rpm/rcf“ is now activated and you can setup the
respective option with the potentiometer.
ATTENTION: Please do not adjust any other centrifuge types than the mentioned one on the
type label of the unit. Otherwise there might the possibility to occur defects, both in the
electronic and the mechanic components.
Here you can adjust the different operation modes stored on the control board.
• Turn the potentiometer until the special digit “service S“ appears in the display “acc/dec“.
Do not confuse with “c“ Submenu Setup of the temperature.
• By pressing the key rpm/rcf the display “rpm/rcf“ is now activated and you can setup the
respective option with the potentiometer.
• As we have already mentioned in the beginning this letters will appear when you switch on the
unit during the normal operation (see chapter 7.3.1).
Here you can adjust or correct the imbalance cut off value. In the factory has been setup an imbalance
cut off value. With this settled value the unit should run through. Through the transport or the setup of
the unit it may occur that this value will react unlike.
In case the unit does already cut off at the adjusted value you can increase this value by adjusting this
value > 0. In case the unit does not cut off until you can decrease this value by adjusting this value < 0.
The suitable value must be settled by service personnel only.
After the correction there is another check indispensable. You have to make several tests.
Activate the operation menu as described under 7.3.1 „Activation of the operation menu (Part 1)“.
Activate the operation menu as described under 7.3.2 „Activation of the service menu (Part 2)“.
Press the button "accel/decel" (5). Turn the potentiometer (1) until the special digit „U“ appears in the
display "acc/dec" (A-2).
Press the button "rpm/rcf" (4). The display "rpm/rcf" (A-1) is now activated and you can choose the
respective setup between -30 and 20 with the potentiometer (1).
Attention: After all points have been reviewed and/or set up, you have to press shortly the key start to
store all parameters. Otherwise the setups were all for nothing. If the storage has been successful in
the display „rpm/rcf“ the value of the imbalance sensor and the word „StorE“ are indicated.
If you want to adjust the mechanical imbalance switch (see chapter 7.4.13), then you have to switch off
the electic movement sensor as follows:
Activate as described under 7.3.1 „Activation of the operation menu" (Part 1)“.
Activate as described under 7.3.2 „Activation of the service menu (Part 2)".
Press the key "time" (6). In the display "rpm/rcf" (A-1) the word "b-SEN, AUS" is now indicated. The
electronical imbalance detector is now not longer activated!
Turn the device off and turn it on again. The word "UA" appears now in the display "time" (A-3),. Now
you can adjust the mechanical imbalance detector, see chapter 7.4.13 "Adjustment of the mechanical
imbalance detector".
After performing any kind of assembling work, please make sure all the grounded contacts are
connected correctly!
7.4.1 Replacing the front housing, the incremental shaft encoder (potentiometer) and the display
• Remove the lower covering by turn out the 2 screws (see photo below).
• Pull the lower edge of the covering away from the mounting plate.
• Loosen the 5 screws under the control panel and pull away the panel forward. Use therefore the
pictured tools.
remove
loosen
Loosen and
pull away
forward
abziehen
• Loosen all electrical contacts to the shaft encoder on the back side of the front housing.
• Loosen all electrical contacts to the display on the back of the front housing.
• Remove the 4 fastening screws of the display and pull away the display upwards.
remove
• Peel off the defective foil keyboard and clean the remaining glued joints.
• Reassemble the control panel in reversed order and mount it back in the front housing.
remove remove
• Remove all screws on the back side of the housing and its electrical grounding.
remove
remove
• Make sure the lid sealing, which is also the rotor chamber sealing, is placed correctly. You can use a
small Allan key.
• Remove the lower covering of the front housing and remove the screws of the housing as described in
chapter 7.4.1 and 7.4.5.
• Pull the housing slightly upwards until the lid gasket is freely accessible.
• Now remove the old lid gasket. ATTENTION: The lid gasket is glued at some points in case you want
to reuse the same gasket you have to loosen the glued points carefully to avoid any damage.
• Take care that the fixing ends are at the back part of the chamber. Start the mounting always with this
glued ends. You can use a small Allan key. Push it between sealing and rotor chamber. Pull the
sealing in a circular movement towards the chamber’s centre (see chapter 7.4.5.).
• Hold the lid with one hand and remove the fixing screws of the hinges.
fixing screws
• Lay the lid with the upper side down on a smooth under packing to avoid any scratches.
• Loosen the screws of the respective hinges on the inner side of the lid.
remove
• Make sure the lid rests on the lid gasket straight and continuous. Tighten the fasting screws firmly at
the backside of the unit after the adjustment.
loosen
• Remove the lower covering of the front housing as described in chapter 7.4.1.
• Remove the connection cable on the control board and remove the aerial.
• Remove the connection cables to the motor on the control board and the frequency converter.
2
1
3
1
1
f
e
• Remove s the areal as described in chapter 7.4.9.
t
• Remove a the screws from the motor fixing.
n
• Lift thezmotor out of the unit.
i
e
h
e
n
• When reassembling the spare motor, take care of the wirings, as they must not be jammed.
• When tightening the motor mount screws, please pay attention the motor rubber mounts are not being
twisted.
• When connecting the motor with the electricity, take care of the rotating direction (Direction of arrow).
• Tilt the unit a little bit and remove the screws of the motor rubber mounts.
• Put in the new motor rubber mounts and reassemble the unit in reverse order.
• Take care that the motor is placed straight and centered in the rotor chamber. The distance of the
center of the motor shaft to the wall of the rotor chamber must be strictly adhered.When tightening the
motor rubber mounts, also take care that they are not being twisted.
remove
• Remove fixing screws as shown on the below picture and remove the frequency converter from the
unit.
remove
• Attention: Before placing a new converter into the unit, put some heat conducting paste onto the fixing
surface. Reassemble the unit in reverse order.
Movement sensor:
• From software version 1.60 it is possible to adjust/correct the imbalance value see chapter 7.3.2.3
• Should appear any difficulties with the imbalance, please give us an exact description of it as well as
the serial number of the unit and the rotor type.
• You can check up the imbalance sensor with the operation menu. Look up in chapter 7.3.1.12.
• Lay the motor down on its side and remove the two fixing screws of the green circuit board on the rear
side (look up in chapter 7.2.8).
• Remove all defective parts as imbalance micro switch, angles or spring plates.
Adjusting screw
Precondition: To adjust the imbalance value, you have to jump the movement sensor with an adapter
at SV2 (speed/imbalance) on the control unit.
You can demand this jumper at Hermle Labortechnik GmbH.
• Put a rotor into the unit and fasten the rotor nut.
• Load one hole of the rotor with the listed imbalance shut off weight in the table 6.3.
• Adjust the acceleration- (accel) and the deceleration level (decel) on the control board to level 9.
• Start the centrifuge. The unit must shut off when reaching the listed speed of the resp. rotor
(see table 6.3).
• In case the unit does not shut off you have to loosen the fixing screws of the imbalance sensor. Adjust
the imbalance sensor more sensitive with the adjusting screw (clockwise).
• Repeat those steps as often as the listed value of the table 6.3 is reached.
• Switch off the unit and fill the rotor with the permitted imbalance weight.
• The unit must not shut off after the start. In case the unit shuts off, you have to lower the sensibility of
the sensor.
• In case you have to readjust the imbalance sensor when testing the permitted imbalance weight, you
have to repeat the procedure with the imbalance shut off.
remove
• Put in the new control board, plug in all electrical connections and reassemble the unit in reverse
order.
Attention:
If you replace the control board you have to do readjust the unit as described in chapter 7.3.2.
• Remove the screws and the center screw of the electronic components mounting plate. Upon this
there are fixed the electronically components.
• Remove the electrical connections of the break resistance to the frequency converter.
remove
• Put in the new break resistance, connect the electrical connections and reassemble the unit in reverse
order.
• Go with a flat, sharp item (knife or screwdriver) under the signal transmitter and lift it up with slight
efforts.
• Fix the new signal transmitter with double-sided adhesive tape and reassemble the unit in reverse
order.
• Remove the two fixing screws of the lid lock and put in the new lid lock.
1
Micro switch for start closing lid
remove
• Keep attention through adjustment of the lid lock, that the lid lies straight and continuous on the lid
sealing. After adjustment tighten the screws well.
Adjustment:
1.) Lay the lid onto the centrifuges seal. Afterwards tighten the screws of the hinges under a little
pressure onto the lid.
2.) Put some washers underneath of the lid lock, in that way that the lid latch is centric to the closing
bar.
3.) Adjust the high of the lid lock, that the distance between the lid and the housing is about 8 mm to
9 mm.
4.) Bent the steel nib of the upper micro switch 1 in that way, that it is given the signal after the lid
latch has pressed the steel nib under the level of the bar. The motor of the lid lock should not be
blocked during the closing of the lock. Otherwise you have to repeat the steps 3.) and 4.).
5.) Perhaps you have to bend the steel nibs of the other micro switches of the end stops into their
position.
6.) Tighten all the screws well.
• Pull out the temperature sensor from the rubber motor cover. Hold therefore the underneath rubber
kink, which held the sensor housing in its position. Press afterwards the rubber kink together and push
it towards the hole.
The expansion valve regulates how much refrigerant is being led to the evaporator. The compressor
makes the refrigerant available in the collective dryer. A sensor is continuously measuring the
temperature at the evaporator output. Through this, the injection nozzle of the expansion valve is
controlled and the evaporator is supplied with the max. necessary amount of refrigerant. Therefore,
maximum capability of the refrigeration system can be reached at any status of operation.
As soon as the required pre-set temperature is reached, the compressor will switch off. When the
temperature rises again, the compressor will switch on again.
The response time of the compressor is controlled with a microprocessor control. The processor
receives the chamber temperature data through a temperature sensor placed in the rotor chamber. As
the actual sample temperature may not be identical to the temperature measured in the rotor chamber,
the micro processor will calculate the sample temperature for the rotor in use. This temperature value is
indicated in the temperature display.
The temperature adjustment of the micro processor is based on this calculated temperature value.
Therefore, the user always gets the exactly requested sample temperature ( 1°C). The samples are not
being damaged through over or lower temperature.
Valve
Photo 31
For this performance you need a refrigerant suction plant with container, a vacuum pump, a vacuum
measuring device, a measuring device for refrigerants and a manometer combination.
• Remove the housing, until the refrigeration set is exposed (see chapter 7.4.1.)
• Connect the vacuum pump, the manometer combination and the refrigerant bottle (R 404 a) to valve 1
(see photo 32).
• Make sure the reached vacuum stays constant after switching the pump off, otherwise the system is
leaky.
• Check all tube connections with a leakage detection device for possible leakage.
ATTENTION:
You must use refrigerant type R 404 a only!
Filling amount is 550 g!
Preface:
The error messages are listed to help localize possible errors faster.
The diagnosis, referred to this chapter may not always be the case, as they are only theoretically
occurring errors and solutions.
Always, please keep us informed about any kind of error occurring, which is not listed in this chapter.
Only through your information we are able to improve and complete this instruction manual.
In case of power failure or malfunction, the lid of the centrifuge can be opened manually in order to
protect your samples.
The error message is shown in the "time" display through particular figures. At the same time the word
“error“ is indicated in the display (see picture).
Error No. 04: Imbalance switch has been activated for longer than 5 seconds
Error No. 11: Temperature sensor is defective (for refrigerated units only)
Error No. 14: Leap of speed is too big between two measurements
(Lid is blocked for … seconds – see below table)
Unit Seconds
Z 216 MK 300
Z 32 HK 600
Z 366 600
Z36 HK 600
Z 326 und Z 326 K 540
20r 300
Seta 2 420
• Cause: Shaft encoder defective or parting of a cable at shaft encoder, possibly loose magnet.
• Solution: Make sure speed magnet has a firm fit, fix it with super glue.
Call service department!
• Cause: The lid of the centrifuge has been opened while centrifuge was running.
• Solution: Close the lid of the centrifuge. DANGER OF ACCIDENT!
Error No. 40: Communication with frequency converter disturbed during start
Error No. 48: Timeout between control unit and frequency converter
Maintenance:
Maintenance of the centrifuge is confined to keeping the rotor, the rotor chamber and the rotor
accessories clean as well as to regularly lubricating the rotor insert bolts of a swing out rotor (if
available). Vaseline, available in nearly each store, is the most suitable lubricant. The Vaseline must be
free of resin and acids. Lubricants containing molycote and graphite are not allowed.
Please pay special attention to anodized aluminium parts. Breakage of rotors can be caused even by
slightest damages.
In case of rotors, buckets or tube racks getting in touch with corrosive substances the concerned spots
have to be cleaned carefully.
• Alkalis
• Alkaline soap solutions
• Alkaline amines
• Concentrated acids
• Solutions containing heavy metals
• Water-free chlorinated solvents
• Saline solutions, e.g. salt water
Cleaning:
Thorough cleaning not only has its purpose in hygiene but also in avoiding corrosion based pollution.
I order to avoid damaging anodized parts such as rotors, reduction plates etc., only pH-neutral
detergents with a pH-value of 6-8 may be used for cleaning.
Alkaline cleaning agents (pH-value > 8) must not be used.
After cleaning, please ensure all parts are dried thoroughly, either by hand or in a hot-air cabinet (max.
temperature + 50°C).
It is necessary to coat anodized aluminium parts with anti-corrosion oil regularly in order to increase their
life-spans and reduce corrosion predisposition.
Due to humidity or not hermetically sealed samples, condensate may be formed. The condensate has to
be removed from the rotor chamber with a soft cloth regularly.
The maintenance procedure has to be repeated every 10 to 15 runs, but at least once a week!
With high g-values, the rate of glass tube breakage increases. Glass splinters have to be removed
immediately from rotor, buckets, adapters and the rotor chamber itself. Fine glass splinters will scratch
and therefore damage the protective surface coating of a rotor.
If glass splinters remain in the rotor chamber, fine metal dust will build up due to air circulation. This very
fine, black metal dust will extremely pollute the rotor chamber, the rotor, the buckets and the samples.
9.1.3 Disinfection
In case of infectious material spilling into the centrifuge, the rotor and rotor chamber have to be
disinfected right after the run. Rotors may be autoclaved at a maximum temperature 121°C, except rotor
220.58 V08, which must not be autoclaved.
The rotor and rotor chamber should be cleaned with a universal, neutral disinfection agent, e. g. on
formalin base. A disinfection spray is most suitable in order to easily reach all difficult to access spots.
ATTENTION:
Before applying any other cleaning resp. decontamination method than recommended by the
manufacturer, contact the manufacturer to ensure yourself, you would not damage the unit or the
rotor by applying the designated method!
Autoclaving
The recommended time for autoclaving: 15 – 20 min at 121°C (1 bar)
ATTENTION: The sterilization time of 20 min. must not be exceeded. Sterilization again and again
will cause reduction of the mechanical resistance of the plastic material.
Before the autoclaving the PP-rotor and adapter must thoroughly be cleaned to avoid the burning in of
dirty residues.
You can disregard the consequences of some chemical residues to plastic materials at ambient
temperatures. But at the high temperatures of the autoclaving those residues may corrode and destroy
the plastic. The objects must be thoroughly washed up with distilled water after the cleaning but before
the autoclaving. Residues of any cleaning liquids may cause fissures, whitening and stains.
Gas sterilization
Boxes, bottles and rotors may be gas sterilized with Ethylene oxides. According to the duration of the
application you may give long enough an airing to the items after the sterilization and before using them
again.
ATTENTION: Because the temperature may rise during the sterilization, rotors, boxes and bottles
must not be closed respectively must be totally unscrewed.
Chemical sterilization
Bottles, boxes and rotors may be treated with the usual liquid disinfectants