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    Results Practical 2

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    Arif Muhamad
    Mucor sp., Aspergillus sp., and Penicillium sp. were observed to have filamentous structures consisting of hyphae and reproductive structures such as sporangiophores, conidiophores, and conidia. Wild fungi were also observed but their specific structures were not documented. An experiment was conducted to count yeast cells under a light microscope using a haemocytometer. A total of 112 yeast cells were counted across 20 squares with an average of 5.6 cells per square. Based on these counts, the total number of yeast cells per mL was calculated to be 224. Additionally, 31 dead yeast cells were counted for an average of 1.55 dead cells per square,

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    Results Practical 2

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    Arif Muhamad
    271 views3 pages
    Mucor sp., Aspergillus sp., and Penicillium sp. were observed to have filamentous structures consisting of hyphae and reproductive structures such as sporangiophores, conidiophores, and conidia. Wild fungi were also observed but their specific structures were not documented. An experiment was conducted to count yeast cells under a light microscope using a haemocytometer. A total of 112 yeast cells were counted across 20 squares with an average of 5.6 cells per square. Based on these counts, the total number of yeast cells per mL was calculated to be 224. Additionally, 31 dead yeast cells were counted for an average of 1.55 dead cells per square,

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    Technique in microbiology II

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    Mucor sp., Aspergillus sp., and Penicillium sp. were observed to have filamentous structures consisting of hyphae and reproductive structures such as sporangiophores, conidiophores, and conidia. Wild fungi were also observed but their specific structures were not documented. An experiment was conducted to count yeast cells under a light microscope using a haemocytometer. A total of 112 yeast cells were counted across 20 squares with an average of 5.6 cells per square. Based on these counts, the total number of yeast cells per mL was calculated to be 224. Additionally, 31 dead yeast cells were counted for an average of 1.55 dead cells per square,

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    271 views3 pages

    Results Practical 2

    Uploaded by

    Arif Muhamad
    Mucor sp., Aspergillus sp., and Penicillium sp. were observed to have filamentous structures consisting of hyphae and reproductive structures such as sporangiophores, conidiophores, and conidia. Wild fungi were also observed but their specific structures were not documented. An experiment was conducted to count yeast cells under a light microscope using a haemocytometer. A total of 112 yeast cells were counted across 20 squares with an average of 5.6 cells per square. Based on these counts, the total number of yeast cells per mL was calculated to be 224. Additionally, 31 dead yeast cells were counted for an average of 1.55 dead cells per square,

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 3

    RESULTS

    Name:

    EXPERIMENT 2.1

    Sample Characteristic

    Filamentous Structure:
    Mucor sp. is a hyphal fungus body structure consists
    of a fine thin thread like tubular branch colony mass
    of mycelium. Unit structure of mycelium is hyphae.
    A hypha is coenocytic.

    Reproductive Structure:
    It consist of sporangiophore containing
    sporangiospores for both asexual and sexual
    reproduction.
    Mucor sp.

    Filamentous Structure:
    It consists of the following components in its cell
    structure that include foot cell, conidiophore, vesicle,
    conidia, mycelium and cytoplasm.

    Reproductive Structure:
    The heterothallic hyphae consist of female hyphae
    that refer to Ascogonium and the male hyphae which
    refer to Anthredium.
    Aspergillus sp.

    Filamentous Structure:
    Species of Penicillium are recognized by their dense
    brush-like spore-bearing structures called penicillin.
    The conidiophores are simple or branched and are
    terminated by clusters of flask-shaped phialides.

    Reproductive Structure:
    The asexual reproduction is by formation of non-
    motile, asexual spores, the conidia, and produce at
    the tip of special, erect, hyphae called conidiophores.
    Penicillium sp.
    Filamentous Structure:

    Wild fungi
    Reproductive Structure:

    EXPERIMENT 2.2

    Figure 2.2 Observation under light microscope


    Table 2.2 Spore counting using haemocytometer
    Total yeast Dead yeast Total yeast Dead yeast
    Square Square
    cells count cell count cells count cell count
    1 9 1 11 5 1
    2 5 2 12 9 0
    3 4 0 13 8 3
    4 6 2 14 6 3
    5 4 1 15 5 4
    6 6 4 16 7 2
    7 8 2 17 6 1
    8 6 1 18 10 0
    9 4 2 19 4 0
    10 7 1 20 7 1

    Total count of yeast cells (all) = 112


    Average number of total yeast cells per square = 5.6
    1 1
    The number of total yeasts per mL = Average yeasts per small square × 0.00025 × 1000 ×
    dilution (10)
    = 224

    Total count of dead yeast cells (all) = 31


    Average number of total dead yeast cells per square = 1.55
    1 1
    The number of total yeasts per mL = Average yeasts per small square × 0.00025 × 1000 ×
    dilution (10)
    = 62

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