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    Ultrasound Imaging SOP

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    AKIN YEMI
    This document provides the standard operating procedure for performing ultrasound imaging using an Ultrasound Biomicroscope system. The procedure involves properly preparing the ultrasound equipment, inducing anesthesia in a mouse, applying ultrasound gel to the mouse's abdomen, obtaining ultrasound images of the target organ, and concluding with cleaning and monitoring the mouse's recovery from anesthesia. Precautions like wearing protective clothing and filling the transducer with distilled water are described.

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    Ultrasound Imaging SOP

    Uploaded by

    AKIN YEMI
    101 views3 pages
    This document provides the standard operating procedure for performing ultrasound imaging using an Ultrasound Biomicroscope system. The procedure involves properly preparing the ultrasound equipment, inducing anesthesia in a mouse, applying ultrasound gel to the mouse's abdomen, obtaining ultrasound images of the target organ, and concluding with cleaning and monitoring the mouse's recovery from anesthesia. Precautions like wearing protective clothing and filling the transducer with distilled water are described.

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    Ultrasound Imaging SOP (2)

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    This document provides the standard operating procedure for performing ultrasound imaging using an Ultrasound Biomicroscope system. The procedure involves properly preparing the ultrasound equipment, inducing anesthesia in a mouse, applying ultrasound gel to the mouse's abdomen, obtaining ultrasound images of the target organ, and concluding with cleaning and monitoring the mouse's recovery from anesthesia. Precautions like wearing protective clothing and filling the transducer with distilled water are described.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    101 views3 pages

    Ultrasound Imaging SOP

    Uploaded by

    AKIN YEMI
    This document provides the standard operating procedure for performing ultrasound imaging using an Ultrasound Biomicroscope system. The procedure involves properly preparing the ultrasound equipment, inducing anesthesia in a mouse, applying ultrasound gel to the mouse's abdomen, obtaining ultrasound images of the target organ, and concluding with cleaning and monitoring the mouse's recovery from anesthesia. Precautions like wearing protective clothing and filling the transducer with distilled water are described.

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    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    of 3

    ULTRASOUND IMAGING SOP

    POLICY:  This procedure must be performed by a trained operator
     
    PRECAUTIONS:   Blue coat, blue overshoes, gloves

    EQUIPMENT:  Ultrasound Biomicroscope (Vevo 770, Visualsonics Inc) 
    25 MHz transducer 
    Distilled water
    Rail system (Visualsonics Inc) 
    Anaesthetizing chamber + Isofluorane / Injectible anaesthesia 
    Animal clipper 
    Hair removal cream (e.g. Nair) 
    Ultrasound gel 
     
    PROCEDURE: 

    1. Turn on the Vevo system using the breaker switch at the back of the system and then the
    computer standby switch on the left side of the system. 
    2. Turn on the monitor. Wait for the Windows operating system to start up. The system
    will automatically log in, and the Vevo 770 software should automatically launch. 
    3. Select the Measurement package to be used. 
    4. Select the appropriate RMV scanhead to be used. Fill the nosepiece as follows:
    i) Ensure that the acoustic window is securely attached and remove the fill port screw. 
    ii) Position the RMV scanhead with the fill port pointing upward. Fill the nosepiece with
    de­ionized water using the supplied water bottle until the nosepiece is almost full. Fill the
    nosepiece with de­ionized water only
    iii)  Using   the   thumb,   cover   the   fill   port   and   tap  the   scanhead   gently   several   times   to
    dislodge any air bubbles in the nosepiece. 
    iiii)  Check to ensure that the face of the transducer does not contain bubbles. If bubbles
    appear, tilt the RMV scanhead to dislodge them. 
    v) Orient the nosepiece so that the remaining air bubbles are placed under the fill port, and
    finish filling the nosepiece with the syringe. 
    vi) After removing all the air from the nosepiece, insert and tighten the fill port screw with
    the screwdriver provided. Be careful to align the fill port screw properly with the threaded
    hole in the nosepiece. Failure to do so may damage the nosepiece. Do not over­tighten the
    fill port screw 
    5. To initialize the desired scanhead, click  Initialize.   Once a scanhead is initialized, the
    Study Browser is displayed. 
    6. Click New and fill out at least the two mandatory study information fields – Operator
    Name and Study Name. 
    7.  Induce anaesthesia with i.p. hypnorm/hypnovel, or with isofluorane at a dose of 5% in a
    closed anaesthetizing chamber then maintained at 2.5% by nose cone for the duration of
    the ultrasound scan.  
    8.  Shave the abdominal fur region of the isofluorane­anaesthetized mouse with an animal
    clipper   first.   Completely   remove   the   remaining   fur   with   a   standard   chemical   hair
    removal cream (Nair) then cleanse the skin thoroughly with water.  Do not allow more
    than 2­3 minutes contact with the animals skin. 
    9. Optional: Inject up to 4mls sterile PBS i.p. to improve ultrasound transmission in vivo.
    10.  Apply ultrasound gel liberally over the abdominal region of the mouse. 
    11.  Place the animal on the heated platform of the rail system. 
    12.  Carefully monitor body temperature, respiration, and anaesthetic depth throughout the
    ultrasound procedure. 
    13. Click  OK to continue with the study. The Vevo 770 will automatically start acquiring
    data in B­Mode. Select:  Setup > B­Mode Setup, or press  <Mode Setup> to change
    acquisition parameters. Use the RMV scanhead to image the organ of interest.
    14. Press   <Scan/Freeze>   to   start   and   stop   data   acquisition.   The   acquired   data   may   be
    reviewed using the cine loop controls at the bottom of the screen. 

    15. For 3D­mode,  connect the RMV scanhead to the 3D motor stage/rail system. Select


    Setup > 3D­Mode Setup or press <Mode Setup> to change the acquisition parameters.
    Click  Init. 3D  to initialize the 3D motor stage. Click  Start 3D. The 3D motor stage
    begins stepping along the acquisition route.
      
    16. To store a data loop select Study > Cine Store, or press ‹Cine Store› 
    17. To store an image select Study > Frame Store, or press ‹Frame Store› 
    18. To add measurements select Tools > Measurements, or press ‹Measure› 
    19. To add annotations, right­click the image, or press ‹Annotate›. 
    20. To review the study data select Study > Browse Study, or press ‹Browse› Export the 
    study's images and loops from the Study menu option or from the Image Browser. 
    21. To save the study, Select Study > Close Study, or press ‹Close› to close the study and 
    click Commit Session Data to save the data. To copy the study to an accessible drive, 
    click Copy To. 
    22.  Once the ultrasound examination is concluded, usually within a 15 min period, clean the
    animal of gel. 
    23.  Switch off the anaesthesia and return the mouse to its cage with food and water ad
    libitum.    Mice normally  recover within 3­5 min from isofluorane anesthesia.    Mice
    anaesthetized with injectible agents will require monitoring until recovery (may be up to
    4 hours)
    24.  At the end of the session click Close Study, or press ‹Close›. 
    25.  Press the computer standby switch on the left side of the system to invoke the Windows
    shut­down process.  
    26.  Click Turn Off from the presented options. 

    DATE ISSUED:   8/3/2010 
    OTHER INFO: http://www.visualsonics.com/products/products_vevo770.htm 

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