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    BIOCHEM Conclusion

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    Gracie Cossette T. Paut
    Amino acids are the building blocks of proteins and possess an amine group, carboxylic acid group, and varying side chains. Qualitative tests use color-producing reagents that react with amino acid side chains to identify the presence of different amino acids. Tests like Ninhydrin, Biuret, Xanthoproteic, Millon's, Hopkins-Cole, Bromine water, Pauly, Reduced Sulfur, and Sakaguchi's reactions were used to identify the presence of tryptophan, tyrosine, histidine, cysteine, and arginine in albumin, and tyrosine, histidine, and arginine in gelatin. This experiment helps teach macrom

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    BIOCHEM Conclusion

    Uploaded by

    Gracie Cossette T. Paut
    89 views1 page
    Amino acids are the building blocks of proteins and possess an amine group, carboxylic acid group, and varying side chains. Qualitative tests use color-producing reagents that react with amino acid side chains to identify the presence of different amino acids. Tests like Ninhydrin, Biuret, Xanthoproteic, Millon's, Hopkins-Cole, Bromine water, Pauly, Reduced Sulfur, and Sakaguchi's reactions were used to identify the presence of tryptophan, tyrosine, histidine, cysteine, and arginine in albumin, and tyrosine, histidine, and arginine in gelatin. This experiment helps teach macrom

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    Amino acids are the building blocks of proteins and possess an amine group, carboxylic acid group, and varying side chains. Qualitative tests use color-producing reagents that react with amino acid side chains to identify the presence of different amino acids. Tests like Ninhydrin, Biuret, Xanthoproteic, Millon's, Hopkins-Cole, Bromine water, Pauly, Reduced Sulfur, and Sakaguchi's reactions were used to identify the presence of tryptophan, tyrosine, histidine, cysteine, and arginine in albumin, and tyrosine, histidine, and arginine in gelatin. This experiment helps teach macrom

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    89 views1 page

    BIOCHEM Conclusion

    Uploaded by

    Gracie Cossette T. Paut
    Amino acids are the building blocks of proteins and possess an amine group, carboxylic acid group, and varying side chains. Qualitative tests use color-producing reagents that react with amino acid side chains to identify the presence of different amino acids. Tests like Ninhydrin, Biuret, Xanthoproteic, Millon's, Hopkins-Cole, Bromine water, Pauly, Reduced Sulfur, and Sakaguchi's reactions were used to identify the presence of tryptophan, tyrosine, histidine, cysteine, and arginine in albumin, and tyrosine, histidine, and arginine in gelatin. This experiment helps teach macrom

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
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    Conclusion

    Amino acids are building blocks of proteins. They possess an amine group, a carboxylic
    acid group and a varying side chain that differs between different amino acids. These side chains
    are essential in determining the presence of amino acids in proteins through qualitative test. The
    qualitative tests conducted in this experiment make use of various color-producing reagents
    which is dependent upon the side chains present in the test samples. Ninhydrin reaction was
    used to confirm the sample as an amino acid or protein. Biuret test was significant in
    distinguishing proteins from amino acids. Xanthoproteic reaction is for classifying amino acids
    with benzene derivative. Millon’s test was used to check the presence of tyrosine in the test
    samples. Both Hopkins-Cole and Bromine water test are important for tryptophan determination.
    Pauly reaction confirms the existence of tyrosine and histidine in the sample. Reduced Sulfur test
    makes use of the sulphur in determining the presence of cysteine and cysteine. And Sakaguchi’s
    reaction is useful in arginine determination. Albumin was confirmed to have the presence of
    tryptophan, tyrosine, histidine, cysteine, and arginine. While for gelatin, only the existence of
    tyrosine, histidine, and arginine were observed.

    This experiment, which is based on the color reactions of amino acids, helps enriching the
    teaching-learning process of macromolecule chemistry experiments. The methodology proposed
    in this lab practice helps students in different practical aspects. The discussion of the reactions’
    topics offers a better understanding of the basic concepts, which allows guiding students in the
    rediscovery of scientific content. These laboratory practices enable students to compile, analyze
    and communicate their results. By this experimental approach students will understand various
    identification tests of amino acids and acquire good skills to perform those experiments in real
    laboratories.

    Ref:

    Elzagheid, M. (2018) Color Reactions of Proteins and Amino Acids. Retrieved from
    https://www.scribd.com/upload-document?
    archive_doc=298944115&escape=false&metadata=
    %7B"context"%3A"archive"%2C"page"%3A"archive_plans"%2C"action"%3A"sta
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    Ilar, Q. (2018). Color Reactions of Proteins and Amino Acids. Retrieved from
    https://www.scribd.com/upload-document?
    archive_doc=298944115&escape=false&metadata=
    %7B"context"%3A"archive"%2C"page"%3A"archive_plans"%2C"action"%3A"sta
    rt_trial"%2C"logged_in"%3Atrue%2C"platform"%3A"web"%7D

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