Agilent 1100MSD GettingStarted

G sbook 4pdf.
fm Pa g e 1 Thu rsday, M a y 14, 1998 1:19 PM
HP 1100 Series
LC/MSD System
Getting Started
Gsbook4pdf.fm Page 2 Thursday, May 14, 1998 8:45 AM
Using Online Help

When you jump to a help topic, it
is displayed in another help
To access the online help, select Help
window. Click the Contents
Topics from the Help menu in any
button in that window to return
window, or click the Help button on any
to this list of help topics.
dialog box.
Item Description
Contents Displays the list of help topics
(shown to the right).
Index Lets you use keywords to
search the help index for a
particular topic.
Find Lets you type a word or phrase
and then displays a list of all
the topics in the online help
that contain those words.
Open Opens the selected book or help
topic. When you open a book or
select a book that is already
open, this button changes to Help Icons
Close.
Print Prints the selected book or help Indicates a book containing more help topics.
topic for future reference. If you To open a book, select it then double-click or
select a book, then all the help click the Open button.
topics contained in the book are
printed. To print a popup, click Indicates an open book of help topics. To close
on it with the right mouse an open book, select it then double-click or
button and select Print from click the Close button.
the menu.
Indicates a help topic. To jump to a help topic,
Cancel Closes the Help Topics window. select it then double-click or click the Open
button.
— 2 —
ChemStation Views
The ChemStation software consists of several Verification (OQ/PV) View
views. The available items in the menus and In this view you can determine if your system is
toolbar depend on the current view. Each view operating in a predictable manner. This is useful
allows you to perform a certain set of tasks. to show Good Laboratory Practice (GLP)
compliance. This may be required by some
You can switch views using the View menu or by government agencies.
using the pulldown menu in the upper left corner
of the screen.
Diagnosis View
This view lets you run tests to diagnose
instrument problems and access information on
how to resolve these problems. The early
maintenance feedback (EMF) feature can be used
to notify you when it is time to perform system
maintenance before a problem occurs. Videos of
the various maintenance procedures are provided
on the LC/MSD maintenance CD-ROM.
Each view is briefly described below and on the
following pages. MSD Tune View
This view lets you calibrate your LC/MSD
Method and Run Control View automatically. You can also set parameters
manually for specific types of molecules.
In this view, you can set up methods and adjust
instrument parameters to inject samples and
acquire data one sample at a time or in
automated sequences.
Data Analysis View

In this view, you can perform various data HP LC/MSD Reference Collection CD-ROM
evaluation tasks on chromatograms and spectra. More information about the ChemStation
You can view both mass spectral and UV signals views and how to use your system can be
simultaneously. Common tasks include found in the ChemStation online help and on
integration, quantitation, checking peak purity, the HP LC/MSD Reference Collection CD-ROM.
and deconvolution. Once you have analyzed the
data, you can select one of the predefined report
types.
Report Layout View

This view lets you design your own custom report
templates to use with the data generated by
your ChemStation.
— 3 —
Method and Run Control View (configured with LC/MSD and DAD)
System
View Selection Title Bar Top Toolbar Diagram
Tool Set Selection
Status Bar
Start/Stop Run
Sampling Diagram
Online Plot
Message Line
Method Overview
Method Pump Column Detector Calibration

System Diagram
Each icon of the system
diagram represents one
component or module of your
system.
Click on an icon if you want to

edit the method parameters
or go to the online help for
that particular component.
Injector Solvent Detector Integration Reporting
— 4 —
Single Sample Tool Set Method Sequence

Lets you work on methods
and run an analysis for a
single sample.
If you click the Single Sample

icon, the toolbar changes as
Move the cursor over individual icons or screen items to
shown.
display descriptive information on the message line.
Sequence Tool Set Method Sequence

Lets you work on sequences
and run automated analyses
of multiple samples.
If you click the Sequence icon,

the toolbar changes as
Move the cursor over individual icons or screen items to
shown. The sampling diagram
display descriptive information on the message line.
now shows the sample tray.
To enable FIA from the system diagram:

Flow Injection Analysis • Click the FIA icon.
Lets you inject multiple • Select Edit FIA Series.
• Check the Enable FIA Series in Method box
samples directly into the and edit the FIA Table.
detector, bypassing the
chromatographic column. The
results are sent to a single
data file. FIA can be used for
method development or for
applications that do not
require chromatography.
— 5 —
Data Analysis View
View Selection Title Bar Cursor Tools Graphics Tools
Tool Set Selection
Signal View Selection
DAD Signal
LC/MSD Signal
Message Line
Integration Tool Set

Lets you perform integration and reporting tasks on a chromatogram.
Click the Integration Move the cursor over individual icons or

Data File Method
icon to display this screen items to display descriptive
toolset. information on the message line.
— 6 —
Calibration Tool Set

Lets you perform calibration tasks for quantitation.
Click the Calibration Move the cursor over individual icons or

Data File Method
Signal Tool Set

Lets you graphically work with the UV or MS signal.
Click the Signals Move the cursor over individual icons or

Data File Method
Spectral Tool Set

Lets you perform spectra evaluation tasks.
Click the Spectral Move the cursor over individual icons or

Data File Method
— 7 —
Data Analysis Tools

Integration Tool Set Calibration Tool Set
Lets you perform integration and reporting tasks Lets you perform calibration tasks.
on a chromatogram.
Create a new calibration table
Integrate all chromatograms
Recalibrate using current chromatogram
Find suitable integration events
Add calibration levels to calibration table
Define integration events
Add new peaks to your calibration table
Specify a report
Define signal options
View a report on screen
Print a report The following cursor tools are for manual

calibration and signal actions.
The following cursor tools are for manual Delete peaks from the calibration table
integration and working in the signal window:
Add peaks to the calibration table
Graphics tools (available in all tool sets)
Recalibrate peaks in the calibration table
Zoom in (available in all tool sets)
Set calibration table options
Zoom out (available in all tool sets)
Show/hide current chromatogram
Pointer tool (available in all tool sets)
Zoom in on MS window
Draw baseline
Extract ion chromatogram
Draw baseline for negative peaks
Hide extracted ion chromatogram
Tangent skim
Split peaks
Delete peak from integration results
— 8 —
Signal Tool Set Spectral Tool Set

Lets you graphically work with a chromatogram. Lets you perform spectral evaluation tasks.
Align the x-axis of multiple signals Set spectral options
Align the y-axis of multiple signals Open a spectral library
Reset the alignment of your signals Save a spectral library
Create a 3D overlay of signals

The following cursor tools are for spectral
selection tasks.
Mirror signals
Select spectrum
Subtract signals
Select peak apex spectrum
Integrate all chromatograms
Select average spectrum
Smooth all signals
Average spectra across a peak
The following cursor tools are for working with
alignment markers and the signal displays. Select peak for purity evaluation
Set a time reference point Select 1st reference spectrum
Move a time reference point Select 2nd reference spectrum
Delete a time reference point Zoom in on MS window
Delete object from chromatogram display Extract ion chromatogram
Display signals overlaid
Display signals separated
Display the signals in full scale
Display all signals in the same scale
— 9 —
Report Layout View
View Selection Title Bar Calculation Technique Report Template Top Toolbar
New Section Menu
Report Section
Insertion Triangle
Click a blue triangle to activate
the new section menu (triangle
turns red)
Message Line
Report Layout Tips

o Move the cursor over the individual icons or screen items
Pointer Tool
to display descriptive information on the message line.
Graphics Tool o Edit or create a report layout as described in the online

help.
Table Tool o To test your report layout, select a calculation technique

from the drop-down list box to define how the results
should be calculated.
Text Tool
o Load a data file. The results are loaded into the report
template using the selected calculation.
Number Tool
o Select File / Add to Report Styles to add your completed
Page Break Tool report template to the list of available report styles. Now
you can use your customized report within a method.
— 10 —
Sample Report Layouts (in \hpchem\repstyle)
Template Description
SIMPLE.FRP Includes a chromatogram and calibration
curves per calibrated compounds.
NESTED.FRP Shows how to nest subsections.
SPECTRA.FRP Shows how to include spectra.
PURITY.FRP Shows how to include peak purity data.
ESTD.FRP Uses the same elements as the standard
Print Preview button
ESTD reports so you can customize your
external standard reports.
AREAPCT.FRP Uses the same elements as the standard
area percent report so you can customize
your area percent reports.
LIBRARY.FRP A customized report that uses library search
results. You must have a method loaded that
specifies the spectra library.
— 11 —
Verification (OQ/PV) View
System
View Selection Title Bar Verification Toolbar Diagram
Status Bar
Start/Stop Run
Sampling Diagram
Online Plot
Message Line
Method Overview
Method Pump Column Detector Calibration

System Diagram
Each icon of the system
diagram represents one
component or module of your
system.
Click on an icon if you want to

edit the method parameters
or go to the online help for
that particular component.
Injector Solvent Detector Integration Reporting
— 12 —
Verification Toolbar
Print a report of the current
The Verification Toolbar, which is located at the instrument verification results.
top of the Verification screen, is displayed when
you select Show Top Toolbar from the View menu.
About This View
Set up a new verification. The Verification (OQ/PV) view lets you test
whether your analytical instruments and the
ChemStation software are operating correctly
according to predefined performance criteria.
Load a previously-stored instrument
verification. • Operation Qualification (OQ)
Operation Qualification is the documented
verification that the equipment-related
Save the current instrument
verification to disk. system or subsystem performs as intended
throughout representative or anticipated
operating ranges.
Edit the current instrument • Performance Verification (PV)
verification.
Performance Verification is the documented
verification that the process and or the total
Run the current instrument process-related system performs as intended
verification. throughout all anticipated operating ranges.
Available Tests
Toggle the display of the Verification
The following verification tests are supplied with
Logbook.
your ChemStation software. Refer to the online
help for more information on how to run, edit, or
Toggle the display of the current create new verification tests.
online Logbook.
o VWD Wavelength Accuracy
o Intensity
Toggle the display of the current o Holmium
Sequence Logbook. o Temperature Accuracy
o Noise, Flow, Temperature
o DAD Wavelength Accuracy
Display the Instrument Verification
o Injector Precision*
Test Report.
o Detector Linearity/Carry-over*
o Injector Linearity*
o Gradient Composition
* These tests can be used to verify LC/MSD performance.
— 13 —
Diagnosis View
View Selection Title Bar Symptoms Possible Causes Cause

Information Pad
Instrument Panel
Variables Display
Message Line
Help
Link to Maintenance
CD-ROM
— 14 —
MSD Tune View
View Selection Title Bar MSD Tune Toolbar
Status Bar
Message Line
Load an LC/MSD tune file. Calibrate the mass axis.
Save the current tune file. Change spray chamber

parameters.
Generate a profile and scan Edit LC/MSD parameters

report. for manual tuning.
Autotune the LC/MSD and Acquire multiple scans and

print a tune report. send results to data file.
— 15 —
To Start Up and Shut Down the System

To Start Up the System To Put the System in Standby Mode
This procedure assumes the system is under Put the system in standby mode overnight or
vacuum and the LC and LC/MSD are properly whenever you won’t be analyzing samples for an
connected. It also assumes the liquid flow path extended time. The standby state for the
is properly set up. LC/MSD leaves the nebulizer and drying gas on at
low flow.
1 From the Method and Run Control view, click
the On button in the system diagram. 1 Flush the system with pure mobile phase to
ensure the flow path is clear of buffers. This
2 Set up the LC conditions (pump, column
avoids plugging the nebulizer. A plugged
heater, and detector).
nebulizer can cause a high back pressure
3 Set up the LC/MSD spray chamber which can damage the LC flow cell.
conditions. If you are using normal flow rates,
the following values are typical. It is good practice to flush the flow path
(including the column) for 5-10 minutes with
Allow 15 minutes for the system to warm up. a mobile phase without buffers such as
50:50 water/acetonitrile.
Electrospray
2 Click the Off button in the system diagram.
All modules will now be set to standby mode
(indicated by the gray color in the system
diagram).
Standby for the LC/MSD is 20 psi for the
nebulizer, 3 L/min for drying gas, 300°C for
drying gas temperature and, if present,
325°C for the APCI vaporizer. Also, the MS
stream selection valve is set LC to Waste.
APCI
— 16 —
To Tune the LC/MSD

What is Tuning? Using Check Tune
When the LC/MSD is used as a detector for the Check Tune lets you quickly determine whether
HPLC, a mass spectrum is associated with each the LC/MSD is correctly tuned without
data point in the LC chromatogram. To obtain performing a complete autotune. It performs a
high quality, accurate mass spectra, the LC/MSD single profile scan of the tune masses and
must be optimized to: compares the peak widths and mass axes with
target values.
• Maximize sensitivity
• Maintain acceptable resolution 1 Select Tune / Check Tune.
• Ensure accurate mass assignment
2 Review the Check Tune report. If any values
are outside of acceptable ranges, Check Tune
Tuning is the process of adjusting LC/MSD
will suggest adjustments.
parameters to achieve these goals. After the
LC/MSD parameters have been optimized, they
must be saved in a tune file (.tun). This tune file Using Manual Tune
is then specified in the method that is used to Use Manual Tune when you want to:
acquire data for your samples. • Achieve maximum sensitivity by sacrificing
some resolution
Frequent tuning, automated or manual, is not
required. Once tuned, the LC/MSD is very stable. • Tune specifically for the very low end (<150
Tuning should generally not be needed more often amu) of the mass range
than monthly, or at most weekly. • Tune with a compound other than the
standard calibrants
Wait at least 4 hours after pumpdown before
tuning or operating your LC/MSD. It takes the Manual tuning involves 4 steps:
analyzer at least 4 hours to reach thermal
equilibrium. Tune files created or data acquired 1 Optimizing ion transmission through the
before the LC/MSD is at thermal equilibrium may
source ion optics (fragmentor, skim 2, lens 1,
have incorrect mass assignments and other
inaccuracies. lens 2, octapole peak and octapole knee).
2 Setting the desired mass resolution
Using Autotune (adjusting width gain and width offset).
Use autotune for automated adjustment of the
LC/MSD performance. 3 Calibrating the mass axis (adjusting mass
gain and mass offset).
1 From the MSD Tune view, select Tune / 4 Adjusting the signal strength (setting iris
Autotune or click the Autotune icon. and adjusting the multiplier gain).
2 Review the tune report which is printed Note that fragmentor and gain are method
automatically when tuning is completed. parameters. The fragmentor affects ion
transmission and fragmentation. For more
information, see the online help.
— 17 —
To Acquire LC/MS Data

Modes of Acquisition 3 Select the method sections you want to edit
There are three modes of acquiring data: (select all sections to become familiar with
the method parameters that are available).
• Running a method for a single sample 4 Add any method comments you want to
• Running a sequence for multiple samples appear on your reports.
• Running an FIA series
5 Set up the instrument parameters:
Note the following about acquiring data: – set up the pump parameters
– set up the injector parameters
• All three acquisition modes require an
– set up the DAD (or VWD) parameters
appropriate method.
– set up the column thermostat parameters
• Samples may be injected either manually or
– set up the MSD signals
with an ALS.
– set up the MSD spray chamber
• A run must always be started from the
software. 6 Set up the Data Analysis parameters:
• An FIA method cannot be used in a sequence. – set up the signal details
– edit integration events
To Edit a Method and Start a Run
– specify report parameters
Once you know the acquisition mode you want to – select the instrument curves
use, you need to set up an appropriate method. – select the calibration curves
Methods are set up in the Method and Run
– set up the calibration table
Control view. New methods are created by editing
existing ones. – find the ion parameters
7 Complete the Run Time Checklist.
1 Select Method / Load Method or click the
Open Method button on the toolbar. Choose a 8 Save the method using a different name.
method from the list. Select Method / Save Method As or click the
Save Method button on the toolbar.
2 Select Method / Edit Entire Method. This
menu item is also available when you click the Once you are familiar with the options that
method icon on the system diagram. are available, you can use the system
diagram menus for quick access to particular
Once you select to edit a method, a series of method parameters, rather than using the
dialog boxes will be displayed that let you set edit entire method process.
up your method and instrument parameters.
9 When you are ready to begin a run, click the
Start button.
Click the Help button on any of the following
dialog boxes for descriptive information on
the items available.
— 18 —
To Use Deconvolution
Deconvolution is a process that transforms 8 You can look at individual components or
mass spectra from multiply-charged ions into a groups of components by selecting specific
calculated molecular weight. components from the Component list.
To Deconvolute a Mass Spectrum 9 Optionally, click the Preview

Deconvolution Report button
This is a generalized procedure; you may need to
do some fine tuning. or click the Print Report button.
1 In the Data Analysis view, load an

electrospray MS data file. The TIC will be
displayed. 10 Optionally, select components from
the Component List box and then click
2 Generate the MS spectrum that you want to the Delete Components button. This
deconvolute (select the Spectral task tool action removes the peaks for the
set and use the spectrum selection tools to selected components from the
pick the spectrum of interest). Note that original spectrum. You can then
continue with step 4 above, using this
these operations are performed on data
new spectrum.
acquired in full scan mode.
11 Repeat the process.
3 Click the Enter Deconvolution Tool
button to set up the deconvolution
display area. Deconvolution Report
The Deconvolution report contains summary
4 When the spectrum is displayed, click information about each component selected,
the Find Ions button to locate
along with detailed information on how each peak
masses that will be used in
in a component contributes to the component’s
deconvolution.
molecular weight. In the first part of the report,
5 Examine the ions that were found. the components are ranked by percent relative
Very noisy data or data with abundance, which is useful for estimating the
unresolved regions often need special percent of impurities.
settings to find ions. You can use the
Find Ions Options button to optimize Note that the actual molecular weight may differ
the set of ions found. from the computed molecular weight even if the
data fit a Gaussian curve perfectly, due to other
6 Click the Edit Deconvolution
errors such as errors in mass axis assignment or
Parameters button to change any of
the parameters. unresolved chemical impurities.
7 Click the Run Deconvolution button

to begin the deconvolution process.
Once the deconvolution is done, the The Deconvolution software is optional and is part
Components will be displayed in the of the HP G2720AA Bioanalysis Software package.
upper right window. The Charge
States will be displayed in the
bottom right window.
— 19 —
Operating Tips
❏ Back up your data and methods regularly to ❏ Use only filtered, HPLC-grade mobile phase.
avoid loss of data if the files are accidentally
overwritten, deleted, or if a hardware problem ❏ If a UV detector is available, use it in series
develops with your disk drive. with the LC/MSD. Try to minimize
chromatographic peak broadening by using
❏ Put the system in standby mode overnight or low dispersion tubing.
whenever you won’t be analyzing samples for
an extended time. ❏ To avoid chromatographic band broadening,
make sure all tubing connections are free of
❏ Make sure the tune file you are using is dead volume. Use zero dead-volume (ZDV)
appropriate for your samples. fittings when possible. If using fingertight
fittings, force tubing into unions and tees
❏ Save Tune reports in an MS Logbook for while tightening the fittings.
future reference.
❏ Use the following table as a guide to using
❏ Regular system maintenance can reduce SIM, condensed scan, and full scan
problems. Maintenance tasks are described acquisition modes.
on the HP 1100 Series LC/MSD Maintenance
CD-ROM. Keep a maintenance record. Task Mode
Acquire electrospray data for Full Scan

❏ Use the Maintenance Logbook and EMF samples containing large, multiply-
features (in the Diagnosis view) to help you charged analytes.
keep track of when maintenance is needed
and to keep an online maintenance record. Analyze a mixture with unknown Scan
components (small molecules).
❏ Flush the sample path and clean the spray Analyze a mixture with known Scan or SIM
components in unknown amounts
chamber, capillary tip, and spray shield daily (quantitate).
or at the end of each shift. Check the pump
fluid level every week. Identify the presence of a few known SIM
compounds at low levels within a
mixture.
❏ The spray chamber vent hose must be
connected to a lab vent that is used only for
the source (completely separate from the ❏ If you are using APCI, the optimal flow rate is
vent hose for the foreline pump). Otherwise, 1.0 ml/min. The range is 0.5 to 1.5 ml/min.
waste products can migrate into the spray
chamber vent producing chemical noise.
❏ Samples need to be filtered. They should be

salt and detergent-free if no
chromatography is used.
— 20 —
Troubleshooting Tips
No peaks High spectral noise
o Make sure there is spray from the nebulizer. o Use appropriate mass filter values.
o Make sure the capillary voltage is set correctly. o Check the spray shape. Nebulizer may be
damaged or incorrectly set.
o Make sure the LC/MSD is tuned correctly.
o Make sure drying gas flow and temperature are
o Make sure LC/MSD pressures are within normal correct for the solvent flow you are using.
ranges.
o Make sure the solvent is thoroughly degassed.
o Check the drying gas flow and temperature. Do not use ultrasonic degassing with protein
o Make sure the fragmentor is set correctly. samples.
o Make sure the LC backpressure is steady; this
Poor mass accuracy indicates a steady solvent flow.
o Recalibrate the mass axis. o If you are using water as part of the mobile
phase, make sure it is de-ionized (>18MΩ).
o Make sure the ions used for tuning span the
mass range of the sample ions and that they
show strong, stable signals. Droplets, not spray, exiting the nebulizer
o Make sure the nebulizing gas pressure is set
Low signal high enough for the LC flow being used.
o Check the solution chemistry. Make sure the o Check the position of the needle in the nebulizer.
solvent you’re using is appropriate for your o Stop the solvent flow and remove the nebulizer
sample. Mixed samples can exhibit signal assembly. Use a magnifying glass to examine
suppression of one or more components. the end of the nebulizer for damage.
o Make sure the sample is fresh and has been
stored correctly. No flow
o Make sure the LC/MSD is tuned correctly. o Make sure the LC is on and there is sufficient
o Check the nebulizer condition. solvent in the correct bottle.
o Clean the capillary entrance. o Check for LC error messages.
o Check the capillary for damage and o Check for blockages. Repair or replace any
contamination. blocked components.
o Check for leaks.
Unstable signal o Make sure the MS stream selector valve is set
to LC to MSD.
o Make sure the drying gas flow and temperature
are correct for the solvent flow you are using.
Undesired fragmentation
o Make sure the solvent is thoroughly degassed.
Do not use ultrasonic degassing with protein o Fragmentor is set too high.
samples. o Ionization is causing fragmentation (APCI vs.
o Make sure the LC backpressure is steady; this Electrospray).
indicates a steady solvent flow. o APCI temperature is too high.
— 21 —
HP 1100 Series LC
Solvent bottles
Solvent cabinet
Vacuum degasser
Pump
Control module
(optional)
Autosampler
Column
compartment
LC Detector
(DAD or VWD)
— 22 —
LC Cabling
Remote cable
CAN bus cable
CAN bus cable
AC power
Analog signal
to recorder
HP-IB to ChemStation
— 23 —
HP 1100 Series LC/MSD — Electrospray
Spray chamber window

Front cover
Electrospray nebulizer release button
Front cover
release button
Electrospray spray chamber
Manual injection valve

(optional)
LC inlet
Spray chamber latch Electrospray
calibrant bottle
On/off switch
— 24 —
HP 1100 Series LC/MSD — APCI
APCI nebulizer Spray chamber window

Front cover
Front cover release button
APCI spray chamber release button
Corona needle
Manual injection valve

(optional)
Spray chamber latch LC inlet
APCI calibrant bottle
On/off switch
— 25 —
HP 1100 Series LC/MSD — Electrospray (with covers removed)
Electronics cabinet
MS selection valve
Vacuum manifold
Electrospray nebulizer
Nitrogen inlet
Electrospray
spray
chamber
Pressure
sensor
Manual injection
valve
Main circuit breaker

Turbo pump
LC inlet
Calibrant delivery system
— 26 —
LC/MSD Cabling — Electrospray (side view, door open)
Calibrant delivery
system gas (N2)
Drying gas (N2) LC/MSD sample line
Gas supply in Nebulizing gas

Drying gas heater
LC tubing
Remote start
Network cable
Spray chamber
high voltage
HP-IB cable
Calibrant delivery
system control
Drain tubes
Foreline pump
power cord Foreline hose
LC/MSD
power cord
— 27 —
HP 1100 Series LC/MSD — APCI (with covers removed)
Electronics cabinet
MS selection valve
APCI nebulizer Vacuum manifold
Nitrogen inlet
APCI spray
chamber
Pressure
sensor
Manual injection
valve
(optional)
Main circuit breaker

Turbo pump
LC inlet
Calibrant delivery system
— 28 —
LC/MSD Cabling — APCI (side view, door open)
Calibrant delivery
system gas (N2) Nebulizing gas
LC/MSD sample line

Drying gas (N2)
Gas supply in
Drying gas heater APCI heater
APCI high voltage
LC tubing
Remote start
Network cable
Spray chamber
high voltage
HP-IB cable
Calibrant delivery
system control
Drain tubes
Foreline pump
power cord Foreline hose
LC/MSD
power cord
— 29 —
Commonly Used Parts — LC/MSD
Description HP Part Number
Chemical Consumables
Calibrant, Electrospray G2421A
Calibrant, APCI G2422A
Evaluation Sample, APCI Negative Mode G2425A
Evaluation Sample, ES Negative Mode G2424A
Evaluation Sample, Positive Mode G2423A
Myoglobin Standard (Multiply-charged) G2426A
OQ/PV Chemical Kit (Caffeine) 8500-6917
Instrument Consumables
Capillary G1946-80009
Corona Needle, APCI G2429A
Electron Multiplier Replacement Horn 05971-801
Ferrule, Nebulizer Needle G1946-20213
Fitting, F120 (Fingertight PEEK Fitting) 0100-1920
Front Capillary Bal Seal, 1/4” ID 0100-1516
Manual Sample Injector 0905-1475
Nebulizer Needle, Electrospray 0101-0941
Nebulizer Needle, APCI G2427A
Nitrogen Gas Conditioner G1946-80047
Tubing (PEEK) 0.005” ID, 1/16” OD 5182-3441
Vacuum Pump Fluid 0890-1915
6040-0834
General Supplies
Abrasive Mesh (micro-grit paper) 8660-0852
Cloth, Clean 05980-60051
Nitrile Rubber Glove, Medium 9300-1751
— 30 —
Safety Warnings
Intended Purpose Safety Symbols
Use of the HP 1100 Series LC/MSD in a manner
not intended by the manufacturer is prohibited.
; Refer to operating instructions.
Safety Class = Indicates hazardous voltages.
+
The HP 1100 Series LC/MSD is a Safety Class 1
instrument and has been designed and tested in Indicates hot surfaces.
accordance with IEC Publication 1010-1, Safety
Requirements for Electrical Equipment for
Measurement, Control, and Laboratory Use. 3 Indicates earth (ground) terminal.
Stack Configuration WA RN IN G
Connecting the LC/MSD to a power source that is not
WA RN IN G equipped with a protective earth contact creates a
While it is possible to stack the HP 1100 Series LC shock hazard for the operator and can damage the
modules on top of the LC/MSD without damaging it, instrument. Likewise, interrupting the protective
this arrangement is potentially unstable and dangerous conductor inside or outside the LC/MSD or
and is not recommended. disconnecting the protective earth terminal creates a
shock hazard for the operator and can damage the
instrument.
Placing the LC modules on top of the LC/MSD is
also inconvenient. It puts the solvent bottles
and some LC controls out of easy reach and
requires the LC stack to be disassembled and
WA RN IN G
removed for LC/MSD maintenance.
Any adjustment, maintenance or repair of the opened
instrument while it is connected to a power source
should be avoided and, if required, should be performed
WA RN IN G only by trained persons who are aware of the hazards
Nitrogen is the only acceptable drying and nebulizer gas. involved.
Use of air, oxygen, or other gases, when combined with
solvents and high voltages in the spray chamber, could
result in an explosion.
WA RN IN G
The power switch on the front of the LC/MSD does not
completely cut power to the instrument. You must also
turn off the main circuit breaker and disconnect the
power cord before performing maintenance on the
electronics or turbo controllers.
— 31 —
H

Document History
First Edition, 5/98

HP G2710 LC/MSD ChemStation Software,
Rev. A.06.01 and later until superseded
*G1946-90035*
*G1946-90035*
Copyright © 1998
Manual Part Number Hewlett-Packard Company
G1946-90035 Printed in U.S.A. 5/98

Agilent 1100MSD GettingStarted

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G sbook 4pdf.

fm Pa g e 1 Thu rsday, M a y 14, 1998 1:19 PM

Using Online Help

Data Analysis View

Report Layout View

Tool Set Selection

Method Pump Column Detector Calibration

Click on an icon if you want to

Injector Solvent Detector Integration Reporting

Single Sample Tool Set Method Sequence

If you click the Single Sample

Sequence Tool Set Method Sequence

If you click the Sequence icon,

To enable FIA from the system diagram:

Data Analysis View

View Selection Title Bar Cursor Tools Graphics Tools

Tool Set Selection

Signal View Selection

Integration Tool Set

Click the Integration Move the cursor over individual icons or

Calibration Tool Set

Click the Calibration Move the cursor over individual icons or

Signal Tool Set

Click the Signals Move the cursor over individual icons or

Spectral Tool Set

Click the Spectral Move the cursor over individual icons or

Data Analysis Tools

Print a report The following cursor tools are for manual

Delete peak from integration results

Signal Tool Set Spectral Tool Set

Align the x-axis of multiple signals Set spectral options

Align the y-axis of multiple signals Open a spectral library

Reset the alignment of your signals Save a spectral library

Create a 3D overlay of signals

Set a time reference point Select 1st reference spectrum

Move a time reference point Select 2nd reference spectrum

Delete a time reference point Zoom in on MS window

Delete object from chromatogram display Extract ion chromatogram

Display signals overlaid

Display signals separated

Display the signals in full scale

Display all signals in the same scale

Report Layout View

New Section Menu

Report Layout Tips

Graphics Tool o Edit or create a report layout as described in the online

Table Tool o To test your report layout, select a calculation technique

Sample Report Layouts (in \hpchem\repstyle)

Verification (OQ/PV) View

Method Pump Column Detector Calibration

Click on an icon if you want to

Injector Solvent Detector Integration Reporting

View Selection Title Bar Symptoms Possible Causes Cause

MSD Tune View

View Selection Title Bar MSD Tune Toolbar

Load an LC/MSD tune file. Calibrate the mass axis.

Save the current tune file. Change spray chamber

Generate a profile and scan Edit LC/MSD parameters

Autotune the LC/MSD and Acquire multiple scans and

To Start Up and Shut Down the System