C hapter 1

Introduction to Biochemistry

Next time you’re at the gym, think about the fact that all the cells in your body
are working together to achieve your goals. Your muscles would rapidly fail without
your liver sending out the sugar they need to contract. You could only last for a
few minutes without your heart pumping oxygen-containing blood throughout your
body. And without your brain sending signals to mastermind it all, you wouldn’t even
make it out of the locker room.
One of the goals of this course is to understand how all the body’s organs work
together during exercise. This is pretty complicated, as you can imagine, so before
we can understand this cooperation between organs, we have to first discuss the
building blocks of the organs, cells. We can reduce cells even further down to their
components, the basic biological building blocks called lipids, carbohydrates,
proteins, and nucleic acids. That’s what biochemistry is all about, trying to
understand the whole from its parts.

Chemistry Review
If you were to take biochemistry, you would first have to take a semester of
introductory biology and four semesters of chemistry. However, our goal in this
course is to learn just enough about biochemistry to understand what’s going on at
the cellular level when we exercise. Let’s first highlight some of the most
important concepts from all those chemistry courses that you (probably) haven’t
taken so that you can follow the rest of this chapter.

A. What is chemistry anyway?

Chemistry is the study of material substances and the changes they undergo.
All materials are comprised of matter, which can be defined as anything that
occupies space and has mass. Matter is composed of extremely small particles
called atoms. There are about a hundred different kinds of atoms in nature. Each
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Figure 1.

different variety of atom is called an element, and chemists arrange these in the
periodic table of the elements (Figure 1). Not all the elements are represented
equally, either on Earth itself or inside cells. All life forms are based on the
element carbon (C), and they also contain relatively large amounts of hydrogen (H),
nitrogen (N), oxygen (O), calcium (Ca), phosphorus (P), and sulfur (S).
Atoms are made up of even smaller particles: positively charged protons,
negatively charged electrons, and neutral neutrons. The arrangement of these
subatomic particles puts the protons and neutrons together in a massive nucleus
with the much smaller electrons buzzing around in a cloud. Atoms have equal
numbers of protons and electrons, which means that the charges cancel and they
are electrically neutral.
Atoms can link together in different ways to form molecules, with the “glue”
that holds them together called chemical bonds. Chemical bonds are often shared
pairs of electrons. The arrangement of atoms in a molecule is called the structure,
and chemists have developed some shorthand ways of representing structures.
As an example, let’s look at the molecule ethanol, which contains two atoms of
carbon, six atoms of hydrogen, and one atom of oxygen. We can write a chemical

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formula for ethanol as C2H6O, where the subscripts reveal how many of each type
of atom is in a molecule. Usually, chemists would draw this molecule using a simple
depiction with symbols for the atoms and lines for bonds (Figure 2C). This type of
drawing can be further simplified by leaving out the explicit bonds between carbon
and hydrogen, since there are so many carbon-hydrogen bonds in biological
molecules (Figure 2D).

B. Oxidation-Reduction Reactions Release Energy

Chemical reactions occur when the atoms in molecules link up in new ways to
produce new substances with different properties than the original materials,
often releasing or consuming energy. Energy produced during chemical reactions
can sometimes be harnessed to do useful work, such as lifting a big weight or
throwing a ball.
An important type of chemical reaction in metabolism is the oxidation-
reduction (or redox for short) reaction. Redox processes enable you to use the
energy within fuel molecules and also provide the energy needed to power your
Ipod and drive your car. Redox reactions involve the transfer of electrons from
one substance to another. The substance that loses electrons is oxidized, while the
substance that gains electrons is reduced. In reactions involving electron
transfer there cannot be an oxidation without a reduction. Note that when an
atom or molecule undergoes oxidation or reduction, it may end up with an unequal
number of protons and electrons, thereby becoming a charged species called an ion.

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Figure 2.5. Salt is made up of oppositely charged particles (ions) that attract each
other. When salt is put into water, the ions separate and interact with water, becoming
dissolved. This happens because each oxygen atom carries a slight negative charge and
each hydrogen atom carries a slight positive charge- meaning that water is a polar
molecule because of oxygen’s greater ability to hold onto the shared pair of electrons.

C. Like Dissolves Like

Cells are pretty much tiny bags of salty water containing a lot of dissolved
molecules and ions. A mixture of water with dissolved substances is called an
aqueous solution. The dissolved materials are called the solutes and the water is
the solvent.
Some materials clearly don’t dissolve well in water. You’ve undoubtedly noticed
that your salad dressing made up of oil-and-vinegar (which is mostly water)
contains two distinct layers until you shake it up. The type of particles within a
mixture dictates whether they will comingle on a molecular level or form distinct
layers. When the particles interact with significant attractive forces, the result is
a homogeneous mixture, with all particles comingled randomly on a molecular level
to form a solution.
Water is composed of an oxygen atom and two hydrogen atoms. Oxygen has a
stronger attraction for the shared pair of electrons than hydrogen, meaning that
there is a partial negative charge on the oxygen and a partial positive charge on

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the hydrogen. Water is therefore a polar compound, meaning that it has a

separation of electric charge. The types of materials that dissolve well in water
are other molecules that have charge separation. Chemists summarize this
phenomenon with the rule of thumb that “like dissolves like.” That is, because
water is a very polar molecule, it dissolves other polar molecules and materials
composed of ions, like salt (Figure 2.5). Substances that dissolve well in water are
called hydrophilic. On the other hand, molecules such as oil, which do not dissolve
well in water, are called hydrophobic. These materials do not dissolve well because
there is no separation of charge between the atoms that comprise them.

D. Acids, Bases, and pH

An important type of molecule in biological systems is an acid. Acids produce H+
in aqueous solution (chemically identical to a proton). An example is hydrochloric
acid, HCl, which splits into its components, H+ and Cl-, in aqueous solution:
HCl  H+ + Cl-
Acids often react with their chemical opposites, bases, which produce OH- in
aqueous solution. An example is sodium hydroxide, NaOH, which splits into its
components, Na+ and OH-, in aqueous solution:
NaOH  Na+ + OH-
Reaction of equal amounts of an acid and a base results in neutralization, or
production of water and a salt:
HCl + NaOH  H2O + NaCl
The pH scale is a way of measuring the amount of acid present in solution. Values for
pH typically fall between 0 and 14.0, with low values meaning an acidic solution, high values
meaning a basic solution, and 7.0 meaning a neutral solution. The pH is related to the
amount of H+ in solution as follows:

pH = – log [H+] Brackets mean that the amount of H+ is

expressed as the concentration in moles
per liter of solution (M).

We can describe a solution as acidic or basic based on the pH as follows:

• Acidic solutions have pH < 7.0.  Basic solutions have pH > 7.0
Bodily fluids such as blood have pH values very close to neutral. Because maintaining
the correct pH is essential for many of the chemical processes in living organisms, acid-
base balance is tightly controlled. Blood pH is normally maintained at 7.4. If the pH rises

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or falls by more than about a half-pH unit, serious illness or death may occur. Excessive
acidity of the blood results in a condition called metabolic acidosis, which can occur during
kidney failure, uncontrolled diabetes, poisoning, and shock. The opposite of acidosis is
alkalosis, which results from excess base (alkali) in the body fluids.
Regulation of acid-base balance occurs in the lungs and kidneys by using a buffer
system that serves to minimize the effects of added acid or base. Blood buffering is
critical, particularly during periods of intense physical activity when lactic acid is
produced.

The Cell
Organisms are composed of one or more cells, the basic units of life. One of the
basic principals of biology is that cells only arise from other cells. That is why our
bodies contain reserves of stem cells, flexible precursor cells that can irreversibly
change into many different types of specialized cells. For example, all the
different blood cell types (red cells and the various types of white cells) are
replaced regularly, arising from a single type of stem cell in the bone marrow.
Scientists are actively investigating the potential of stem cells to grow into new
tissues to replace those damaged by injury or diseases like Alzheimer’s.
There are about 200 different kinds of cells in the human body, and each adult
human contains about 1013 cells all working together. Different types of cells
display a wide variety of shapes and sizes. Let’s focus on the key features found in
a “generic” animal cell (Figure 3). The cell is enclosed by the plasma membrane,
which forms the boundary that separates the cell from its environment. Only cells
of higher organisms, the eukaryotes, contain interior compartments called
organelles that are also enclosed by a membrane.
Key parts of a eukaryotic cell include the following:
• the nucleus, where the genetic material containing the information necessary
for the synthesis of all cellular molecules is found.
• the mitochondria, where fuels are oxidized for energy. On average each cell
contains hundreds of mitochondria.
• the cytosol, the watery solution of proteins, nutrients, and other molecules
in which the organelles are suspended.

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Thousands of chemical reactions occur throughout the cell, and careful

regulation of all these processes must exist for the good health of the organism.
When regulation runs amuck, diseases such as cancer are often the result. In the
next sections, we look in detail at the molecules within the cell that are responsible
for the tightly coordinated processes that occur there.

Lipids
Overview of Lipids Lipids are defined by their
solubility. They are not
Waxes, fats such as lard, and oils belong to the
soluble in water.
general class of biological molecules called lipids.
Although biological lipids are structurally quite diverse, none of them dissolve well
in water because they hydrophobic. Lipids play a number of key roles in the cell,
representing stored chemical energy, comprising the basic structural units of
membranes, and carrying signals between cells.

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Figure 4

Energy Storage Lipids: Triglycerides

Most of the energy reserves in humans and other animals are stored in the form
of triglycerides, lipids that contain a core molecule called glycerol bonded to three
fatty acids (Figure 4). Triglycerides are efficient energy storage molecules for
two major reasons. First, they are highly reduced, thereby releasing a lot of
energy when they are burned. Second, because they are hydrophobic, the
molecules pack closely together without carrying water molecules, reducing the net
weight. Carbohydrates, the other principal form of stored chemical energy in living
organisms, are highly hydrated, meaning that they are stored with associated
water molecules that add to the weight but not to the energy content.
Triglycerides form oily droplets in the
watery environment of the cell. Humans
and some other animals contain
specialized fat cells (adipocytes) that are
almost entirely filled with triglycerides
(Figure 5).
Triglycerides also serve other useful
purposes in animals. Polar bears, seals,
walruses, and other warm-blooded animals
living in extreme climates have a thick
layer of fat under the skin to insulate
them against the cold. Camels contain a
large reserve of triglycerides in their triglycerides
humps. Like all hydrocarbons, combustion
of triglycerides produces carbon dioxide Figure 5. Fat cells (adipocytes)
and water. Thus, the “ship of the desert” contain a large droplet of insoluble
triglycerides.
metabolizes its lipid stores to obtain both

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water and energy. We will examine how triglycerides are oxidized to release energy
when we consider energy production during metabolism.

Figure 6

Membrane Lipids: Phospholipids

The boundary between the inside and the outside of a cell (or an organelle) is
composed of a double layer of lipids called the membrane (see Figure 3). Biological
membranes are comprised largely of phospholipids, similar in structure to the
triglycerides except that one of the fatty acids is replaced by a “head group”
containing a charged phosphate group (Figure 6).
When placed in water, phospholipids spontaneously assemble into the lipid
bilayer that forms the foundation of membranes (Figure 7). Because “like dissolves
like”, the charged head groups interact with water on the outside of the structure
and the greasy fatty acid tails interact with each other on the inside of the
structure. This structure provides a barrier that is relatively impermeable to
hydrophilic molecules because of the hydrophobic interior. On the other hand,
hydrophobic molecules can effectively “dissolve” in the interior, allowing them
penetrate the membrane and get inside the cell.

Rigid Lipids: Steroids

Animal membranes also contain cholesterol, a member of the steroid family. All
steroids are based on a common structure consisting of a fused-ring system

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Figure 7

(Figure 8). This structure is very different from the phospholipids shown above
and provides rigidity to the membrane. Up to 25% of the lipids of some membranes
can be cholesterol. The single polar group of cholesterol is the –OH, which lines up
with the polar head groups of the phospholipids in a membrane. The rest of the
molecule inserts itself into the hydrophobic interior of the membrane. Other
steroids also play important biological roles, particularly in signaling. Familiar
examples include the sex hormones, estradiol and testosterone, which we will talk
about more later in the course.

Figure 8 Steroids are a family of compounds that share the fused-ring

structure on the left. One example is cholesterol, shown on the right.

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Carbohydrates Carbohydrates are often

Overview of Carbohydrates called sugars.
Carbohydrates, commonly known as sugars, are
quite polar and water-soluble. Carbohydrates are the most abundant biological
molecules on Earth and have a variety of cellular functions, including in energy
storage and structure.

Monosaccharides
The building blocks of carbohydrates are the monosaccharides. Common
monosaccharides include glucose, blood sugar, and fructose, fruit sugar.
Monosaccharides can react with other monosaccharides to form disaccharides
(containing 2 monosaccharide units; Figure 9), oligosaccharides (3-19 units), and
polysaccharides (>20 units).

Polysaccharides
Polysaccharides that contain repeating units of glucose (glucose polymers) are
important energy storage and structural molecules in both plants and animals
(Table 1). Two important
glucose polymers are cellulose
and starch, which chemically
differ only in how the glucose
subunits are linked together
(Figure 10). Cellulose has the
bonds pointing up, and starch
has the bond pointing down.
Although this may seem like a
minor difference, the two are Figure 9. Joining the two monosaccharides
dramatically distinct. Cellulose, glucose and fructose leads to the
the principal component of disaccharide sucrose , common table sugar.

wood, is water-insoluble and indigestible (to animals). About a third of all plant
matter is cellulose: it provides the scaffolding that holds up most plants, giving
rigidity to their cells. On the other hand, starch, which is made by plants to store
energy for future use, is an easily digestible compound widely enjoyed in potatoes,
pasta, and bread. Humans make a similar molecule for energy storage called
glycogen. Cellulose and starch illustrate the principle that structure affects
function in biological systems.

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repeating unit of starch repeating unit of cellulose

Figure 10. Starch and cellulose are both polymers of glucose. They differ only in
whether the connecting oxygen atom points down as in starch or up as in cellulose.

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Proteins Proteins are chains of

Overview of Proteins amino acids linked
together.
Proteins have a diverse range of functions in the
cell, including nutrient storage, catalysis, immunity, transport, signaling, and
structural roles (Table 2). In this section, we first examine the general structures
of proteins and then look at some examples in more detail.

Amino Acids and Peptides

Proteins are polymers constructed from
twenty different building blocks called amino
acids, much as words are constructed from
letters. Amino acids all contain an amine and a
Figure 11. Proteins are polymers
carboxylic acid group but vary in the identity composed of amino acid subunits.
of the “side chain” (Figure 11). Side chains An amino acid contains an amine
have different chemical properties (Figure group (blue), a carboxylic acid
12), ranging from polar to hydrophobic groups. group (red), and a variable side
Amino acids react with other amino acids to chain R (purple). In the cell, the
form peptides. amine group exists as NH3+ and
the carboxylic acid as COO-.

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Figure 12. The structures of the twenty common amino acids.

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An example of a peptide is aspartame, also known as the sugar substitute

Nutrasweet (Figure 13). Other small peptides also have biological activity,
including some signaling compounds in the body. The enkephalins, naturally
occurring pain relievers made in the brain (endorphins), are pentapeptides,
containing five amino acids. Some peptides, such as oxytocin, act as hormones.
Oxytocin has powerful biological activity, inducing labor in pregnant women and
stimulating the release of milk during nursing.

Figure 13. Some biologically relevant peptides. Aspartame is approximately 200

times sweeter than sugar. Leucine enkephalin is a naturally-occurring pain reliever
in the brain. Oxytocin controls contraction of uterine muscle.

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Sequence of amino acids determines

the structure of the protein.

Fig 14 A polypeptide chain spontaneously folds into the correct three-

dimensional shape with most of the polar and charged amino acids (green) on
the surface interacting with water and most of the hydrophobic amino acids
(yellow) in the nonpolar interior.

Polypeptides
When many amino acids link together, a polypeptide is formed. Proteins consist
of one or more polypeptide chains. After a polypeptide chain is synthesized in the
cell, it folds into a unique three-dimensional shape (Figure 14). Folding is guided by
such forces as “like dissolves like” with hydrophobic amino acids typically
interacting with other hydrophobic amino acids in the interior and polar amino acids
interacting with water on the exterior.

Structure Determines Function

One of the fundamental principles of biochemistry is that the order of amino
acids in the polypeptide chain (the sequence) determines the three-dimensional
shape, or structure, of a protein. That is, a particular protein always has the same
sequence of amino acids in the same organism and thus the same structure. The
key to the action of many proteins in the cell is specific binding to another
molecule- this binding is often the result of the two molecules “fitting together”
appropriately. Certain amino acids are then poised in exactly the right position to
carry out crucial chemical reactions (Figure 15).

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Fig 15 A protein (purple) binds its target molecule (orange) at a

specific site to position a reactive amino acid side chain (blue) appropriately
for chemistry to occur.

Enzymes
Thousands of chemical reactions must occur in Enzymes act as catalysts,
a typical cell in order to maintain life. Many of increasing the rate of a
reaction of a substrate without
these reactions would occur much too slowly
being consumed themselves.
without the aid of protein catalysts called
enzymes, which can lead to rate enhancements of up to 1017. Note that a rate
enhancement of 1017 means that a reaction that takes one second in the presence
of an enzyme would take over 3 billion years without the enzyme.
An enzyme is specific for a particular target molecule, called the substrate,
much as a key fits only a certain lock. Again, the three-dimensional structure of
the protein is crucial for its function. Through a variety of chemical strategies,

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enzymes induce their substrate to undergo a particular chemical reaction, much as

a key opens a lock. Like the key, the enzyme remains unaltered by the reaction.
Many poisons and pharmaceuticals act by inhibiting enzymes. One example is the
nonsteroidal anti-inflammatory drugs (NSAIDS), which include many compounds in
the athlete’s medicine cabinet (such as ibuprofen, acetominophen, and aspirin).
These compounds inhibit a specific enzyme involved in the production of
prostaglandins (Figure 16). Prostaglandins are hormones that act locally to produce
diverse effects in the body, including transmission of pain information to the brain,
modulation of the thermostat in the hypothalamus, and inflammation. Blocking the
production of these messengers means that the NSAIDs have many beneficial
effects in the body, including reduction of pain, fever, and inflammation.

Fig 16

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Figure 17 General structure of an antibody, which binds specifically to its

target antigen. Using this binding mechanism, an antibody tags a foreign
invader, like a microbe, for attack by other parts of the immune system.
Antibodies are produced by white blood cells.

Antibodies
The action of the immune system is mediated by another class of proteins called
antibodies. Antibodies are composed of four polypeptide chains, two “heavy” chains
and two “light” chains, which interact to form a Υ-shaped structure (Figure 17).
Antibodies bind to specific molecules called antigens, which are often part of
disease-causing microorganisms. Specificity arises from the amino acids at the tips
of the Υ, which differ between antibodies and are therefore called the “variable”
regions. Rather than inducing their antigens to undergo chemical reactions like
enzymes, antibody binding triggers a massive immune response that results in
annihilation of the invader.

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Fig 18 Addition of antibodies specific to the carbohydrate antigens on

a red blood cell results in antibody-antigen binding and agglutination, visible
as an insoluble precipitate in the bottom of the tube.

Antibody responses are vital to help us fight infectious disease but can have
disastrous consequences, as in the case of the rejection of blood of the wrong
type. Specific carbohydrates on the surface of red cells determine blood type.
These carbohydrates also act as antigens for specific antibodies found in people
with other blood types. For example, people with type A blood have the A antigen
on the surface of their red cells and antibodies in their bloodstream against the
type B antigen. If type B blood is injected into a person with type A blood, these
antibodies will perceive the type B blood cells as invaders and target them for
destruction by the immune system. Additionally, antibody binding to the B-antigens
triggers clumping of the foreign cells into an insoluble mass, a process called
agglutination (Figure 18). Serious consequences may result for the patient,
including shock, kidney failure, and death.
Before blood types were discovered in 1900, doctors experimented with blood
transfusions in patients dying from severe blood loss, often transferring blood
directly from a healthy donor. Sometimes these treatments saved lives and other
times they hastened death when the blood was of the wrong type. Discovery of

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ABO blood types was a key step in the ability to use transfusions routinely in
medical procedures.
The agglutination reaction is now used to determine blood type. Specific
antibodies are added to a sample of blood. The antibody that clumps the cells
together to form a solid that falls out of solution (a precipitate) reveals the blood
type. Before receiving a transfusion today, a patient’s blood is routinely typed to
avoid problems with blood incompatibility.

Structural Proteins
Proteins also play a variety of structural roles. Muscle, hair, skin, and connective
tissue are largely comprised of proteins with three-dimensional structures well
suited for their functions. Amazingly, the same protein, keratin, comprises not only
hair and skin but also wool, nails, claws, quills, horns, and hoofs. The degree of
hardness of the resulting material is dependent on the number of covalent linkages
involving the amino acid cysteine. Two cysteines form an S-S covalent bond (a
disulfide linkage) that links keratin strands together to form multi-stranded
fibers. In the toughest keratin filaments, such as those comprising rhinoceros
horn, almost one in five amino acids is a cysteine involved in a disulfide linkage.

Abnormal Protein Structures

A recurring biochemical theme is that protein structure determines function.
Occasionally, there is an error, or a mutation, in a crucial amino acid of a protein.
Mutations can be a recipe for disaster. An example is hemoglobin S, in which the
nonpolar amino acid valine replaces the polar amino acid glutamate on the protein
surface, resulting in an unfavorable interaction with water (Figure 19). Instead, a
new interaction with another hemoglobin molecule results. The net result is an
aggregation of hemoglobin molecules to insoluble fibers that cause red blood cells
to “sickle” and then burst under certain conditions.
Individuals who inherit hemoglobin S from both parents can suffer greatly from
this disorder, called sickle cell disease, which can even result in death. Ironically,
possession of one copy of this type of hemoglobin is an advantage to those who live
in regions plagued by malaria, such as Africa and the Mediterranean, because the
malarial parasite cannot survive in red blood cells that contain hemoglobin S.
Many other genetic diseases are the result of a protein that contains a single
“wrong” amino acid, leading to the loss of functional protein. How does the
structure of a protein go wrong? Typically, it is an error in the gene that codes for
that protein, leading us to the last type of biological building block, the nucleic
acids.

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Figure 19. Left: The presence of a single hydrophobic amino acid on the surface of
hemoglobin (top) results in individual molecules clumping together (middle), eventually
forming long, insoluble fibers (bottom). Right: The result is “sickling” of red cells to an
elongated shape that can lead to painful “log jams” in blood vessels.

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Nucleic Acids
Overview of Nucleic Acids
The instructions to assemble each protein in DNA is the genetic material.
the cell can be found in the genetic material, We inherit 23 chromosomes
comprised of the nucleic acids, DNA and RNA. from each parent.
Deoxyribonucleic acid (DNA) is the carrier of
genetic information, while ribonucleic acid (RNA) plays a role in using the
information contained within the DNA to make new molecules in the cell. Some
viruses use RNA as their genetic material instead of DNA.
DNA and RNA are both polymers of nucleotide subunits (Figure 20) linked by
phosphate groups to form long chains. Inside the nucleus, DNA interacts with
proteins for tight packaging so that the 3-meter long chains can fit inside such a
small space (Figure 21). With few exceptions, each cell in the human body contains
3 billion nucleotide units assembled into nuclear DNA. Mitochondria also contain
their own DNA, which is present as a circular molecule of about 17,000 nucleotide
units.

Fig 20

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Fig 21

Structure of the Gene

Two chains of DNA form a double helix in which the chains run in opposite
directions with the sugar-phosphate “backbone” on the outside and the bases on
the inside. Relatively weak interactions, called hydrogen bonds, between adenine
and thymine and between guanine and cytosine act as “glue” to hold the structure
together. The charged phosphates on the outside of the structure interact
favorably with the polar water of the cell.
While people have long noticed that certain characteristics are passed on from
generation to generation, it was not until the early twentieth century that
scientists realized that certain diseases can also be hereditary. This observation
was made by Sir Archibald Garrod (1857-1936), an astute English physician
studying the disease alkaptonuria, one symptom of which is production of black
urine. Garrod demonstrated unequivocally that alkaptonuria is inherited and
proposed the novel idea that it results from a defective enzyme leading to
improper metabolism of the black compound. Normal individuals break down this
compound so that it does not appear in the urine.
Garrod’s work implied a link between DNA, the molecule of inheritance, and
enzymes, the cell’s catalysts. In the 1940’s, it was proposed that a single region of
DNA called a gene codes for a single enzyme (the “one gene-one enzyme”
hypothesis). This proposal has been modified only slightly in the last sixty years.
The next step was to determine how DNA codes for a biological product.

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Cracking the Code

The mechanism by which a DNA sequence is interpreted in the cell is extremely
complex, involving a number of biological molecules working cooperatively. There
are two major steps in the process: transcription, in which an intermediary
molecule called a messenger RNA (mRNA) is created from the DNA template, and
translation, in which the instructions carried by the mRNA are used to create the
corresponding protein product (Figure 22). We will not discuss the details of this
process, but it’s important to know that the sequence of the nucleotides
determines the sequence of the protein that’s made

Figure 22. In the process of transcription, the sequence of one of the DNA strands
(the gene) is “read” by the cellular machinery to create a new messenger RNA (mRNA)
molecule. The mRNA is “complementary” to the DNA in the same way that the two DNA
strands are complementary to each other. For example, if the coding strand has a G,
then the mRNA has a C. Because RNA uses U instead of T, an A in the DNA becomes a
U in the mRNA. In the process of translation, the sequence of the mRNA is interpreted
by the ribosome in three base units as a specific amino acid. The ribosome links each
successive amino acid to the growing chain.

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