American International Journal of Biology

June 2014, Vol. 2, No. 2, pp. 01-09

ISSN: 2334-2323 (Print), 2334-2331 (Online)
Copyright © The Author(s). 2014. All Rights Reserved.
Published by American Research Institute for Policy Development

Floral Biology and Pollination Ecology of Okra (Abelmoschus

Esculentus L. Moench)

Professor Olugbenga E. Ige1 and Eludire M. O.2

Abstract

The floral biology and pollination ecology of three varieties of okra (Abelmoschus
esculentus, L. Moench) was carried out. They are: long okra (lady’s finger), short okra
(spine okra), and L.S 45. The investigation revealed that L.S 45 was the first variety
to germinate on the 4th day followed by the two remaining varieties that germinated
on the 5th day. From germination to flowering, the varieties showed morphological
differences and similarities in growth rate, height, and flowering period. The flower
structure combines hermaphrodism and self compatibility and the anthesis took
place at night. The flower opened at dawn; remain opened all morning and closed in
the evening. It is wilted in the evening and the petals fall the next day. There were
significant similarities in the pollen morphological structures and in terms of pollen
productivity per flower, var. long okra produced the highest.

Keywords: Floral Biology, Ecology, Pollination, Pollen

Introduction

Okra, Abelmoschus esculentus L. (Moench), is an economically important

vegetable crop grown in tropical and sub-tropical parts of the world. This crop is
suitable for cultivation as a garden crop as well as on large commercial farms.

It is grown commercially in several parts of the world but India ranks first in
the world with 3.5 million tonnes (70% of the total world production) of okra
produced from over 0.35 million ha land (FAOSTAT 2008).

1 Department of Pant Science and Biotechnology, Adekunle Ajasin University, Adekunle Ajasin
University, Akungba Akoko, Nigeria. Email: [email protected],
Phone Number: +2348067108282.
2 Department of Plant Science and Biotechnology, Adekunle Ajasin University, Akungba Akoko.
2 American International Journal of Biology, Vol. 2(2), June 2014

Okra is cultivated for its fibrous fruits or pods containing round, white seeds
and is mainly propagated by seeds and has duration of 90-100 days. Okra plants are
characterized by indeterminate growth. Flowering is continuous but highly dependent
upon biotic and abiotic stress. The okra pods are harvested when immature and high
in mucilage, but before becoming highly fibrous. Generally the fibre production in the
fruit starts from 6th day onwards of fruit formation and a sudden increase in fibre
content from 9th day is observed (Nath, 1976). Okra plants continue to flower and to
fruit for an indefinite time, depending upon the variety, the season and soil moisture
and fertility. Infact the regular harvesting stimulates continued fruiting, so much so
that it may be necessary to harvest every day in climates where growth is especially
vigorous.

The fruits are eaten as a vegetable. Okra fruit can be cooked in a variety of
ways. The roots and stems of okra are used for cleaning the cane juice from which gur
or brown sugar is prepared (Chauhan, 1972). Its ripe seeds are roasted, ground and
used as a substitute for coffee in some countries. Mature fruits and stems containing
crude fiber are used in the paper industry. Extracts from the seeds of the okra is
viewed as alternative source for edible oil. The greenish yellow edible oil has a
pleasant taste and odor, and is high in unsaturated fats such as oleic acid and linoleic
acid. The oil content of the seed is quite high at about 40%. Okra provides an
important source of vitamins, calcium, potassium and other mineral matters which are
often lacking in the diet of developing countries (IBPGR, 1990). The composition of
edible portion of okra is given below (Gopalan et al., 2007).

This study is aimed at determining the growth rate of each variety at regular
basis, the time of flowering and the floral characteristic as well as the pollen
productivity.

Materials and Methods

The materials used were plastic buckets, okra seed, loamy soil, that was filled
into the bucket as a growth medium. The seeds collected from three different varieties
of okra (A. esculentus) were sourced from the Agricultural Development Program,
Ministry of Agriculture, Ikare-Akoko, Ondo State,Nigeria.

These seeds are long okra (lady’s finger), short okra (spinned okra), and L.S
45. The seeds have the same size and shape, with their color ranging from ash-brown
to black.
 Ige & Eludire 3

The study was conducted at the green house of the Plant Science and
Biotechnology Department, Adekunle Ajasin University, Akungba Akoko, between
October and December 2010.

The method used for planting was the pot evaluation method. Plastic pots or
buckets (with diameter and depth of about 10cm to 20cm) were used. This method
was found to be reliable (Singh et al, 1990). The plastic pots were filled with top soil
and arranged at the green house of the department.

The planting of seed were done at 3-5 seeds in the bucket, these three varieties
of okra were planted into different buckets with 5 replicates each. The buckets were
arranged in rows, each row consisting of three buckets and its replicates.

Spacing between each row was about 20-30cm. The okra seeds were planted
with about 3-5 seeds in each pot in a depth of 2-4cm and were watered moderately
and regularly.

Flower Morphology and Anthesis

The flower morphology was described using the inflorescence of each variety.
The morphology of the floral parts, the different colours of the flower and anthesis of
the varieties were monitored. The time of anthesis initiation and termination in flower
of each variety, the time of flower opening and closing daily were also observed and
recorded.

Collection of Fresh Samples (Flower)

Mature flower heads of each variety of okra were collected. The flowers were
stored in plastics containing glycerine to prevent desiccation and growth on samples.

Pollen Slide Preparation

Acetolysis method (Erdtman’s, 1969) was used in preparing the pollen

analysis. The anthers collected were transferred from the vials to calibrated centrifuge
tubes.
4 American International Journal of Biology, Vol. 2(2), June 2014

This method involved the introduction of acetolysed mixture comprising of

acetic anhydride mixed with concentrated Sulphuric acid (H2SO4) to the centrifuge
materials in the plastic test tubes. The anthers were crushed using glass rods, different
glass rods were used; one glass for each sample to avoid contamination of one sample
by another.

Volume of the samples were determined and read, after knowing the volume
of the sample, drops of glycerine was added and the samples were then transferred
into storage vials from where they were mounted on each slides.

Mounting and Counting

10µl of the sample was placed on a scale using micropipette and a cover slip
over it. Three slides were prepared for each variety. The slides were observed using
X40 objective lens, and counting was done on X40 objective lens because the grains
were large in size. The pollen in each of the three slides were counted and an average
count determined.

The photomicrographs of the selected pollen grains of the three varieties were
taken at different view with the aid of the Leitz Wetzlar camera attached to the
Microscope. Furthermore, pollen measurement for each variety was noted and a total
of thirteen grains were measured for each variety.

Results

Flower Morphology

The flowers are borne vertically, and its axillary and solitary, on a peduncle 2.0
– 2.5 cm long. The flowers are about 2 inches in diameter, with five white to yellow
petals with a red or purple spot at the base of each petal. The flowers were almost
actinomorphic. The perianth consisted of 5 sepals and 5 distinct petals. The
androecium consisted of very many monadelphous stamens which bears filaments.
The gynoecium was a single pistil consisting of several carpels, and a superior ovary.

The calyx, corolla and stamens are fused together at the base and fell off as
one piece after anthesis.
 Ige & Eludire 5

Pollination Ecology

Venkatramini (1952), observed that flower bud initiation, flowering, anthesis

and stigma receptivity are influenced by genotype and climatic factors like
temperature and humidity. From studies made on three okra varieties, flower buds
are initiated at 22-26 days and the first flower opened 41-48 days after sowing. Once,
initiated flowering continues for 40-60 days. Anthesis was observed between 6 a.m.
and 10 a.m. Anthers dehisce before flower opening, and hence self pollination may
occur at anthesis. The dehiscence of anthers is transverse and complete dehiscence
occurs in 5-10 minutes (Purewal and Randhawa, 1947).

Stigma receptivity was also observed the day before flowering (50-70%) and
the day after (1-15%). Flowers opened only once in the morning and closed after
pollination on the same day and in the following morning, the corolla withered.

Although insects are unnecessary for pollination and fertilization, the flowers
are very attractive to bees and the plants are cross-pollinated. The cross pollination up
to the extent of 4-19% (Choudhury et al.,1994; Shalaby, 1998) with maximum of
42.2% (Mitidieri and Vencovsky, 1998) has been reported. The extent of cross
pollination in a particular place will depend upon the cultivar, competitive flora, insect
population and season.

Germination and Growth Rate

The planting date of the varieties were recorded as they began to germinate,
the growth rate of each variety in each row were observed, measured and also
recorded.
6 American International Journal of Biology, Vol. 2(2), June 2014

The data on the plant height were taken each day and later at weekly interval
then on monthly basis. The germination and growth rate are represented in the tables
1 and 2.

Table 1: Showing the Germination Rate of the Varieties

Days Long Okra Short Okra L.S 45

Replicates Replicates Replicates
1ST 2ND 3RD 1ST 2ND 3RD 1ST 2ND 3RD
4TH - - - - - - 1 - -
|5TH 1 - 1 - - 1 2 1 1
6TH 2 1 1 - 1 1 2 2 1
7TH 2 2 3 2 1 2 2 3 3
8TH 2 2 3 2 1 2 2 3 3

Table 2: Showing the Average Growth Rate in Height

Days Long Okra Short Okra L.S 45

Replicates Replicates Replicate
1ST 2ND 3RD 1ST 2ND 3RD 1ST 2ND 3RD
(cm) (cm) (cm) (cm) (cm) (cm) (cm) (cm) (cm)

4TH - - - - - - 1.0cm - -
5TH 2.00 - 1.80 - - 1.90 1.50 1.40 1.60
6TH 2.60 2.00 2.50 1.50 2.00 2.50 2.10 2.00 2.30
7TH 3.50 3.20 3.60 2.60 2.2. 3.10 3.00 3.10 3.10
8TH 3.80 3.20 3.60 2.80 2.60 3.20 3.00 3.20 3.30

WEEKS
1ST 4.00 3.50 3.80 3.00 2.80 3.50 3.00 3.20 3.30
2ND 11.00 10.00 9.00 8.00 9.00 7.50 7.00 8.00 6.80
3RD 17.00 16.00 16.50 15.00 17.00 16.50 16.00 15.00 17.00
4TH 22.00 20.50 21.00 19.00 24.00 20.00 20.00 22.00 21.50

MONTHS
1ST 22.00 20.50 21.00 19.00 24.00 20.00 20.00 22.00 21.50
ND
2 26.00 24.60 25.00 24.00 26.00 25.00 23.00 25.00 24.00
3RD 32.00 30.00 29.00 31.00 28.00 29.50 28.00 33.00 29.00
Ige & Eludire 7

Table 3: Number of Days of Flowering of Each Variety

Long Okra Short Okra L.S 45

Replicate Replicates Replicates
1ST 2ND 3RD 1ST 2ND 3RD 1ST 2ND 3RD
55 60 59 62 58 62 41 38 46
DAYS DAYS DAYS DAYS DAYS DAYS DAYS DAYS DAYS

Table 4: Number of Pollen Grains Per Anther/Flower

Varieties Average Volume Amount of No of No No of

Pollen/10µl of Pollen in A Grains ofanther Pollen
Glycerol = Amount Produced on Produ
in Which Produced/ by 1 Flower ced /
Pollen 5 Anthers Anthers Flowe
Was r
Kept=A
LONG 10 O.8ml 800 160 5 800
OKRA
SHORT 1 0.8ml 80 16 5 80
OKRA
L.S 45 0 0.4m 0 0 5 0

Table 5: Pollen Morphology

Varieties Exine Pattern Apertural Characteristic

LONG OKRA spinate with pila evenly Polyporate
distributed around pores
and spine
SHORT OKRA Spinate, same as long Polyporate
okra
L.S 45 - -

Discussion

From planting to germination, the varieties showed some morphological

differences and similarities as well.
8 American International Journal of Biology, Vol. 2(2), June 2014

Table I showed the growth rate of the okra varieties which differ from each
other; var. L.S 45 were the first to germinate on the fourth day of planting, on the
fifth day of planting, growth of var. Short Okra and Long Okra was observed. Variety
L.S 45 recorded high number of germinated seedlings followed by variety Long okra
and short okra.

Height of okra varieties was showed in Table 2. Week 1 showed that there
was no significant difference between var. L.S 45 and var. Short Okra while there was
a significant difference between var. Long Okra and the two other varieties.

There was also variation in the flowering period of the three varieties.
Flowering begins 38 days after planting with var. L.S 45 emerging as the first to
flower, var. Long Okra started to flower 55 days after planting and variety Short Okra,
58 days after planting. Table 3 showed details of the flowering date of the varieties
and their replicates.

In all varieties, flower opening was initiated between the hours of 6:00am -
6:30am and closes between 11:30am -12:00pm . At anthesis, self-fertilization is far
from complete and only a few ovules are then fertilized. Ovules continue to be
fertilized during the morning and allogamy may be possible. The possibility of
allogamy decreases rapidly and a pollen grain deposited on the style after midday has
no impact on progeny. In okra, stigmas are exposed to allopollination at anthesis, and
only anthers in the upper ring come into contact with stigmas. The moment of
contact depends on the initial distance between these and the rate of elongation of the
stamen sheath. In a given variety, the number of ovules available depends on the
number of ovules per carpel and on the number of carpels per fruit. These are varietal
characteristics. All the Abelmoschus species bear fruits with five carpels.

Okra produce nectars which attract insects to them, various insect such as
Butterflies, Houseflies, Honeybees were seen around the okra flower. Ant was
observed to be the most prominent out of the various insects observed. Ant was
observed to start visiting the flower immediately after opening is initiated. Pollen grain
of okra are heavy and sticky and could not be readily transferred by wind, therefore
insect are responsible for the transfer of pollen grains and consequently cross
pollinated in okra plant.
 Ige & Eludire 9

Pollen productivity refers to the quantity of pollen produced by anther as per

flower or per inflorescence. The average sizes of pollen grain ranged between 135.0
and 162.5(polar view) and 125.0, 127.50 to 160.0(equatorial). Both long okra and
short okra belonged to the class of large pollen grain according to Erdtman(1969).

The pollen morphology showed some similarities in the exine structure (i.e.
pattern) and apertural characteristics.

Conclusion

This study showed the differences and similarities between the three varieties
of okra. Var. L S 45 was the first to germinate, produce flower and produce pods and
can therefore be recommended for commercial purpose due to the ease of production
and fast maturity rate.

However, the pollen morphology of the varieties showed slight differences.

Var. long okra has the highest number of pollen compared to other varieties in terms
of pollen productivity and can be therefore be recommended in conditions where
large seed production is required.

References

Chauhan, D.V.S. (1972). Vegetable Production in India, 3rd ed., Ram Prasad and Sons (Agra).
Choudhury.B and Choomsai M. L. A. (1994). Natural cross-pollination in some
vegetable crops. Indian J. Agric Sci. (40) (9): pp 805-812
Erdtman, G. (1969). Handbook on palynology. An introduction of the study of pollen grains
and spores
FAOSTAT., 2008. (http://www.fao.org)
Gopalan. C., Rama Sastri. B.V. and Balasubramanian .S., (2007). Nutritive Value of
Indian Foods, published by National Institute of Nutrition (NIN), ICMR
Mitidieri, S and Vencovsky, K. (1998). Rev. Agric (Piracicaba Braz) 49(1):3
Nath, V. O. (1976). Taxonomic relationships and breeding possibilities of species
related to okra (Abelmoschus esculentus). Bot. Gaz 113, pp 455-64
Purewal, S. S. and Randhawa, G.S. (1947), Studies in Hibiscus esculentus (Lady’s Finger).
Chromosome and pollination studies. Indian J. Agri. Sci, 17, 129-136.
Shalaby, G.J., 1998. Natural cross-pollination in okra. Journal on Agriculture Science 3(1):
381-386.
Venkitaramani, K. S., (1952). A preliminary study on some inter varietal crosses and hybrid
vigour in Hibiscus.167pp.