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    Dichloran Rose Bengal Chloramphenicol Agar (DRBC Agar) : Composition

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    This document provides information about Dichloran Rose Bengal Chloramphenicol Agar (DRBC Agar), including its composition, directions for preparation and use, principle and interpretation, quality control tests and results, storage instructions, and a reference. DRBC Agar is a selective and differential medium used for isolation of fungi and yeasts from food samples.

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    Dichloran Rose Bengal Chloramphenicol Agar (DRBC Agar) : Composition

    Uploaded by

    sandhy krisna
    156 views2 pages
    This document provides information about Dichloran Rose Bengal Chloramphenicol Agar (DRBC Agar), including its composition, directions for preparation and use, principle and interpretation, quality control tests and results, storage instructions, and a reference. DRBC Agar is a selective and differential medium used for isolation of fungi and yeasts from food samples.

    Original Description:

    media Agar DRBC

    Original Title

    M1881

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    This document provides information about Dichloran Rose Bengal Chloramphenicol Agar (DRBC Agar), including its composition, directions for preparation and use, principle and interpretation, quality control tests and results, storage instructions, and a reference. DRBC Agar is a selective and differential medium used for isolation of fungi and yeasts from food samples.

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    156 views2 pages

    Dichloran Rose Bengal Chloramphenicol Agar (DRBC Agar) : Composition

    Uploaded by

    sandhy krisna
    This document provides information about Dichloran Rose Bengal Chloramphenicol Agar (DRBC Agar), including its composition, directions for preparation and use, principle and interpretation, quality control tests and results, storage instructions, and a reference. DRBC Agar is a selective and differential medium used for isolation of fungi and yeasts from food samples.

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    © All Rights Reserved
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    of 2

    Dichloran Rose Bengal Chloramphenicol Agar (DRBC Agar) M1881

    Dichloran Rose Bengal Chloramphenicol Agar (DRBC Agar) is used for selective isolation of fungi-yeasts and moulds of
    significance in food spoilage
    Composition**
    Ingredients Gms / Litre
    Peptic digest of animal tissue 5.000
    Dextrose 10.000
    Monopotassium phosphate 1.000
    Magnesium sulphate 0.500
    Rose Bengal 0.025
    Chloramphenicol 0.100
    Dichloran 0.002
    Agar 15.000
    Final pH ( at 25°C) 5.6±0.2
    **Formula adjusted, standardized to suit performance parameters

    Directions
    Suspend 31.6 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving
    at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates

    Principle And Interpretation


    Dichloran Rose Bengal Chloramphenicol Agar (DRBC Agar) is formulated by as described by King et.al (1) and is
    recommended for selective isolation of yeasts and moulds especially in food samples.
    This medium is a modification of Rose Bengal Chloramphenicol Agar which additionally contains dichloran.
    Peptic digest of animal tissue provides nitrogen, vitamins and minerals. Dextrose is a carbohydrate source. Phosphate buffers
    the medium. Magnesium sulfate provides divalent cations and sulfate. Dichloran is an antifungal agent, added to the medium
    to reduce colony diameters of spreading fungi. Rose Bengal exhibits an improved inhibitory activity at pH 5.6 and hence the
    final pH of the medium is maintained at 5.6 for the inhibition of spreading fungi (1) The presence of rose bengal in the medium
    suppresses the growth of bacteria and restricts the size and colonies of the more rapidly growing moulds. Chloramphenicol
    is included to inhibit the growth of bacteria present in environmental and food samples. Inhibition of growth of bacteria
    and restriction of spreading of more-rapidly growing moulds aids in the isolation of slow-growing fungi by preventing their
    overgrowth by more-rapidly growing species. Additionally Rose Bengal is taken by yeast and moulds colonies , which allows
    these colonies to be easily recognized and enumerated.
    This medium should not be exposed to direct light as rose bengal undergoes photo-degradation leading to formation of toxic
    chemicals for fungi (2,3).

    Quality Control
    Appearance
    Light yellow to pink homogeneous free flowing powder
    Gelling
    Firm,comparable with 1.5% Agar gel
    Colour and Clarity of prepared medium
    Pink coloured, clear to slightly opalescent gel forms in Petri plates
    Reaction
    Reaction of 3.16% w/v aqueous solution at 25°C. pH : 5.6±0.2
    pH
    5.40-5.80

    Please refer disclaimer Overleaf.


    HiMedia Laboratories Technical Data

    Cultural Response
    Cultural characteristics observed after an incubation at 25-30°C for upto 6 days.
    Cultural Response
    Organism Inoculum Growth Recovery
    (CFU)
    Cultural Response
    Bacillus subtilis ATCC 6633 >=10³ inhibited 0%
    Candida albicans ATCC 50-100 good-luxuriant >=50%
    10231
    Escherichia coli ATCC >=10³ inhibited 0%
    25922
    Mucor racemosus ATCC good-luxuriant
    42647
    Saccharomyces cerevisiae 50-100 good-luxuriant >=50%
    ATCC 9763

    Storage and Shelf Life


    Store between 15-25°C in tightly closed container and the prepared medium at 2-8°C . Use before expiry period on the label.

    Reference
    1.King D.A. Jr., Hocking A.D. and Pitt J.I., 1979, J. Appl. Environ. Microbiol., 37:959.
    2.Sharp A.N. and Jackson A.K., 1972, J. Appl. Bact., 24:175.
    3.U.S. Food and Drug Administration, 1995, Bacteriological Analytical Manual, 8th Ed., AOAC International, Gaithersburg,
    Md.

    Revision : 2/ 2013

    Disclaimer :
    User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in this
    and other related HiMedia™ publications. The information contained in this publication is based on our research and development work
    and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to specifications
    and information related to the products at any time. Products are not intended for human or animal diagnostic or therapeutic use but for
    laboratory, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not be considered
    as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.

    HiMedia Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147
    1919 Email: [email protected]

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