Ezp System
Ezp System
Ezp System
Zeta Plus ™
Encapsulated Filter System
Innovative Filtration.
Fast, easy and clean.
The system of choice for single-use depth filtration
The Zeta Plus™ Encapsulated System is a single-use depth filtration system designed
for the bioprocessing industry where upstream cell culture clarification and impurity
removal or downstream impurity reduction is required. The Zeta Plus Encapsulated
System consists of small and medium systems (model 16EZA) that are ideal for lab
scale production or scale up studies and a large system that is designed for production
scale biomanufacturing. Each system is comprised of a filter holder, top and bottom
Zeta Plus™ Encapsulated standard capsule
manifolds and the required number of capsules.
offering with translucent polycarbonate shells
The Zeta Plus Encapsulated Systems for production scale biomanufacturing feature
an ergonomically designed large holder (model 16EZB) that can be pivoted between
horizontal and vertical positions, allowing for convenient loading and unloading,
minimal footprint during filtration, minimal fluid spills during unloading and full
utilisation of the filter media.
The capsule design features a translucent plastic shell (standard capsules) for easy
liquid level detection. A self guiding locking mechanism enables fast and robust
capsule-to-capsule connection and the industry leading 3M Purification Zeta Plus
Zeta Plus™ Encapsulated capsule offering with depth filter media provides superior filtration performance. If sanitation using NaOH
alkaline resistant* polyphenylene/polystyrene solution is required, capsules with alkaline-resistant shells are available.
shells
* Based on testing with 1M NaOH and 5% NaClO (Bleach). Ask for Chemical Compatibility Guide for more information.
2
Zeta Plus™
Encapsulated System
Applications
• Clarification of mammalian cell culture harvest
• Endotoxin reduction
The primary application for the Zeta Plus Encapsulated System is the cell culture
clarification after fermentation. The System can be employed alone or in combination
with centrifugation or Tangential Flow Filtration (TFF). An alternative application
for the Zeta Plus Encapsulated System is downstream impurity reduction. Taking
advantage of its positive charge capacity, Zeta Plus filter media has been shown to be
effective in removing contaminants such as host cell proteins (HCP), viruses, DNA,
protein aggregates and endotoxins.
In monoclonal antibody production, Zeta Plus filter media is increasingly being used Zeta Plus™
as a polishing step after Protein A column capture step, replacing traditional anion Multi-Round
bespoke
exchange chromatography.
customised
system
3
Figure 1: Zeta Plus™ Encapsulated large Ergonomically designed filter holders
holder loading process Traditional depth filtration systems utilise lenticular style cartridge filters and a vertical
filtration flow path to allow easy access to process liquids and efficient utilisation of
filter media. However, stacking cartridges from bottom to top can be cumbersome and
dismantling used cartridges is often labour intensive. Furthermore, fluid spills during
the unloading process could be problematic and, in some instances, might expose the
operators to the process fluids.
Recognising the need for a depth filtration system that is fast, easy and clean, 3M
Purification designed filter holders (Model 16EZB) that can be pivoted between
the horizontal position for loading and unloading the capsules and manifolds and
the vertical position for filtration. Allowing loading and unloading at waist height
eliminates the need for operators to lift capsules above their heads and reduces the
risk of fluid spills when handling used capsules. The use of the vertical flow path
allows for full media utilisation and a small system footprint during filtration.
05SP01A
10SP02A
30SP02A
60SP02A
60SP05A
90SP05A
60ZA05A
90ZA05A
90ZA08A
120ZA08A
10 6 4 2 1 0.5 0.1
Micron
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Zeta Plus™
Encapsulated System
Top Bottom
Manifold Manifold
5
Figure 5: Single-use manifold and capsule Scalability
filter schematics The Zeta Plus Encapsulated System retains the lenticular filter design and vertical
Upstream vent flow path that are characteristics of traditional depth filtration systems, helping ensure
Downstream vent consistent performance from bench-scale to production scale and from traditional
non-disposable to disposable systems.
Manifold stop
Four different surface area disposable scale-up devices are available for media grade
selection and filter sizing. The BC25 capsule with 25 cm2 filter is ideal for media grade
screening. The scale-up capsule filters with 170 cm², 340 cm² and 1020 cm² are designed
for intermediate scale-up studies and lab-scale protein production. Combined with
O-rings
these small-scale devices, the Zeta Plus Encapsulated System offers a completely
Male connector single-use solution to process from 0.5 litre to over 5000 litres of liquid.
Top Manifold
Female connector Table 2a: Zeta Plus™ Encapsulated System capsule filter and manifold specifications
Configuration
Small capsule Large capsule
Filter capsules
Alkaline Alkaline
Standard Standard
resistant1 resistant1
Dimensions (height x diameter) 5.7 cm x 45.2 cm 20.3 cm x 45.2 cm
Weight
Outlet Manifold stop Dry 3.3 kg 3.4 kg 10.0 kg 10.7 kg
Inlet
Wet post blow-down 4.4 kg 4.8 kg 19.3 kg 19.7 kg
Bottom Manifold
Materials of construction
Filter media Filter aids, cellulose, binding resin Filter aids, cellulose, binding resin
Polyphenylene / Polyphenylene /
Outer shell Polycarbonate Polycarbonate
Plastic shell Polystyrene Polystyrene
O-rings Silicone Silicone
Separators, spacers and
Polypropylene Polypropylene
connectors
Female Edge seals Thermoplastic elastomer Thermoplastic elastomer
connector
Handles N/A Nylon
Hold-up volume
Capsule fill Double layer ~ 1.1 litre ~ 5.2 litre
E16 Single-Cell Capsule
0.23 m² capsule with double-layer or single-layer media volume2 Single layer ~ 2.6 litre ~ 4.2 litre
Post blow-down hold-up volume3 < 100 ml < 100 ml
Straight Maximum operating line pressure 3.4 bar 3.4 bar
Female handle
Maximum differential pressure
connector 2.4 bar 2.4 bar
forward
T-handle 1 cycle autoclave (30 min.) 1 cycle autoclave (30 min.)
Sterilisation
at 126 °C at 126 °C
Double layer: 1.6 m²
Effective filtration area 0.23 m²
Single layer: 2.5 m²
Top/Bottom manifold
Capsule
Plastic shell stop Dimensions (height x diameter) 5.2 cm x 45.2 cm 5.2 cm x 45.2 cm
Connector 1.5” sanitary style 1.5” sanitary style
1.6 m² Capsule
1.6 m² capsule with double-layer media Polyphenylene / Polyphenylene /
Material Polycarbonate Polycarbonate
2.5 m² capsule with single-layer media Polystyrene Polystyrene
Weight 4.5 kg 4.5 kg 4.5 kg 4.5 kg
Hold-up volume < 250 ml < 250 ml
1
Based on testing with 1M NaOH and 5% NaClO (Bleach). See Chemical Compatibility Guide for more information.
2
Capsule fill volume is defined as the volume of liquid that is required to fill the portion of the capsule outside the filter media.
3
Post blown-down hold-up volume is defined as the volume of the residual liquid after nitrogen gas blown-down.
6
Zeta Plus™
Encapsulated System
Table 2b: Zeta Plus™ Scale-Up Capsule specifications Figure 7: Scale-Up Capsule filter schematics
Configuration
Filter Capsules Inlet
170 cm² 340 cm² 1020 cm²
Dimensions
Height x diameter 10.3 cm x 21.6 cm 15.2 cm x 21.6 cm
Weight
Dry Single layer 1.0 kg 1.0 kg 1.4 kg
Double layer 1.0 kg 1.0 kg 1.6 kg
Wet post blow - Single layer 1.1 kg 1.1 kg 1.8 kg
Outlet
down Double layer 1.2 kg 1.3 kg 2.4 kg Vent
Figure
re 6: The Zeta Plus
Plus™ single
single-use
use depth filtration product portfolio
16EZB
(DL 11.2 m²)
(SL 17.5 m²)
or .3
x 2 1.7
act x
f
ling
a 2000 - 25000 L
Sc
16EZA
(DL 0.23 m² to 4.8 m²)
x3 (SL 0.23 m² to 7.5 m²)
x2 200 - 5000 L
E1020
(1020 cm²)
E0170 25 - 600 L
x7 (170 cm²)
E0340
(340 cm²) 5 - 25 L
BC25
(25 cm²)
1-5L
0.5 - 1 L Process Volume
7
Table 3: Zeta Plus Encapsulated System Table 4: Zeta Plus™ Encapsulated System holder specifications
holder capacity Holder model
Single stage Small and medium holders Large holder
Model E16 Single-cell E16 Multi-cell (Model 16EZA) (Model 16EZB)
capsule capsule Maximum operating pressure 3.4 bar 3.4 bar
16 EZA 4 1 Materials of construction
16 EZB N/A 7 Frame 304 stainless steel 304 stainless steel
Two-stage End plate 304 stainless steel 304 stainless steel
Model E16 Single-cell E16 Multi-cell Support rods 440 stainless steel 316 stainless steel
capsule capsule Stand 304 stainless steel 304 stainless steel
16 EZA 2 N/A Hand wheels 300 Series stainless steel 300 Series stainless steel
16 EZB N/A 6 Gear box N/A Epoxy-coated cast iron cover
Locking bar N/A 304 stainless steel
Casters Stainless steel Stainless steel
Wheels Phenolic Polyurethane
Surface finish
Standard 1.0 μm 1.6 μm
Minimum
94.3 cm (1 high) Minimum
122 cm (2 high) 120 cm (3 high)
Maximum Maximum
117 cm (1 high)
145 cm (2 high) 148.5 cm (3 high)
Depth
Width & Depth 49.2 cm
Width
66 cm 50 cm
Maximum
210.7 cm 205.9 cm
113.9 cm
86.4 cm
108.7 cm
Vertical position Horizontal position
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Zeta Plus™
Encapsulated System
NOTES:
1. All Zeta Plus™ EXT grades are available for order.
2. For further information regarding Zeta Plus™ EXT Series filter media, please contact your local 3M Purification representative.
3. DEL108A is a Zeta Plus™ EXT grade with two single layers of Delipid (DEL1) media.
4. Single layer 05SP media is offered in 0.23 m² and 1.6 m² Capsule format. One part number example is E16E07A05SP.
A-2a. Single-layer standard capsule filter ordering information A-2b. Alkaline resistant* single-layer capsule filter ordering
information
Single-cell capsules Multi-cell capsules Single-cell capsules Multi-cell capsules
Product description 3M ID number Product description 3M ID number Product description 3M ID number Product description 3M ID number
E16E01A30LA 70020288430 E16E11A30LA 70020288604 E16R01A30LA 70020288802 E16R11A30LA 70020289032
E16E01A30LP 70020288448 E16E11A30LP 70020288612 E16R01A30LP 70020288810 E16R11A30LP 70020289040
E16E01A50LA 70020288471 E16E11A50LA 70020288646 E16R01A50LA 70020288836 E16R11A50LA 70020289073
E16E01A50LP 70020288489 E16E11A50LP 70020288653 E16R01A50LP 70020288844 E16R11A50LP 70020289081
E16E01A60LP 70020288505 E16E11A60LP 70020288687 E16R01A60LP 70020288877 E16R11A60LP 70020289115
E16E01A90LP 70020288539 E16E11A90LP 70020288729 E16R01A90LP 70020288901 E16R11A90LP 70020289156
E16E01ADELI 70020256130 E16E11ADELI 70020288588 E16R01ADELI 70020288786 E16R11ADELI 70020289016
* Based on testing with 1M NaOH and 5% NaClO (Bleach). Ask for Chemical Compatibility Guide for more information.
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Zeta Plus™
Encapsulated System
A- 170 cm² Scale-up capsule filter ordering information A- 340 cm² Scale-up capsule filter ordering information
3a. Double-layer capsules 4a. Single-layer capsules 3b. Double-layer capsules 4b. Single-layer Capsules
Description ID number Description ID number Description ID number Description ID number
E0170FSA05SP01A 70020290170 E0170FSA30LA 70020290246 E0340FSA05SP01A 70020290543 E0340FSA30LA 70020290618
E0170FSA10SP01A 70020290196 E0170FSA50LA 70020290303 E0340FSA10SP01A 70020290568 E0340FSA50LA 70020290675
E0170FSA10SP02A 70020290204 E0170FSA30LP 70020290253 E0340FSA10SP02A 70020290576 E0340FSA30LP 70020290626
E0170FSA30SP02A 70020290279 E0170FSA60LP 70020290345 E0340FSA30SP02A 70020290642 E0340FSA60LP 70020290717
E0170FSA60SP02A 70020290378 E0170FSA90LP 70020290436 E0340FSA60SP02A 70020290741 E0340FSA90LP 70020290808
E0170FSA90SP05A 70020290451 E0170FSADELI 70020290139 E0340FSA90SP05A 70020290824 E0340FSADELI 70020290501
E0170FSA60ZA05A 70020290410 E0340FSA60ZA05A 70020290782
E0170FSA90ZA05A 70020290485 E0340FSA90ZA05A 70020290857
E0170FSA120ZA08A 70020290220 E0340FSA120ZA08A 70020290592
E0170FSADELI08A 70020290147 E0340FSADELI08A 70020290519
E0170FSADELP08A 70020290154 E0340FSADELP08A 70020290527
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HEREUNDER WITH RESPECT TO INFORMATION OR THE PRODUCT TO WHICH INFORMATION REFERS.
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the 3M Purification product is fit for a particular purpose and suitable in your specific application.
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OF ANY NATURE WHATSOEVER RESULTING FROM THE USE OF OR RELIANCE UPON INFORMATION.
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