mAU

FT E
3000
2500
2000
Thermo Scientific
A280

1500
1000
500
Pierce FPLC Purification
0
0.0 10.0 20.0 30.0 40.0
Table of Contents

Introduction
FPLC Cartridge Overview 1
Introduction
Product 1
Highlights 1
Thermo Scientific Products 2-3
Fusion Protein FPLC Purification 2-3
Introduction to Product
His-tagged Protein FPLC Purification 4-7
2
Product Protocol
GST-tagged Summary
Protein FPLC Purification 4
3
More Product 4
Antibody
Even More FPLC Purification
Product 4-6
5
Again, Even
Protein A More Product 6-7
4
Protein
ProductG 2 4
8
Protein A/G 5
Product Protocol Summary 8
Protein L 5
More Product 8
Melon Gel 6
Product 3 9-10
Phosphoprotein FPLC Purification 6
Product Protocol Summary 9
More Product 10
Biotinylated Protein FPLC Purification 7

Protein Desalting using FPLC 8

 FPLC Cartridge Overview

Chromatography Cartridge Highlights:

• Two sizes – 1mL and 5mL, convenient for typical research scales
• Compatible – fittings allow connection with popular LC systems
or a standard syringe
• Versatile – use singly or connected in series to service different
capacity requirements
• Validated – available cartridges have been tested to ensure
performance in the format
• Reusable – accessory pack includes caps for convenient
storage between uses
• Economical – comparable performance at lower cost than other
commercially available cartridges

Thermo Scientific Pierce Chromatography Cartridge properties.

  1mL Cartridge 5mL Cartridge

Dimensions 0.7 x 2.7cm 1.3 x 3.8cm
Thermo Scientific Pierce Chromatography Cartridges are Recommended Flow Rate 1-4mL/min 1-7mL/min
convenient, reliable, ready-to-use, pre-packed 1mL and 5mL Maximum Pressure 0.3mPa (43 psi or 3 bar) 0.3mPa (43 psi or 3 bar)
columns of sample-prep and affinity purification resins for
Cartridge Material Polypropylene Polypropylene
manual or automated liquid chromatography (LC).
Frit Material Polyethylene Polyethylene
The cartridge fittings are compatible with the popular automated
liquid-chromatography systems or for manual syringe processing.
The cartridges attach directly to ÄKTA® or FPLC Systems without Applications for Pierce Chromatography Cartridges:
additional connectors. Cartridges can be used individually or con- • His-tagged protein purification
nected in a series to obtain even higher column capacity. Each • GST-tagged protein purification
product supplied in the Pierce® Chromatography Cartridge format • Antibody purification
includes an accessory pack that readily adapts cartridges for use • Biotin binding
using Luer-Lok® Syringe Fittings or tubing. The cartridges provide
• Phosphoprotein enrichment
fast, easy and reproducible chromatographic separations and can
be regenerated for multiple uses. • Protein desalting

Port Diameter: 4.2mm

10-32 threaded coned port
17.5mm

Port Diameter: 4.2mm

10-32 threaded coned port
20mm
12.5mm

13.5mm
89mm

75mm

14mm

8.5mm

4mm 4mm

Thermo Scientific Pierce FPLC Cartridges schematic.

To order, call 800-874-3723 or 815-968-0747. Outside the United States, contact your local branch office or distributor.
1
 FPLC Cartridges

His-tagged Protein FPLC Purification Thermo Scientific HisPur Cobalt Cartridges

Highly selective binding for the highest purity His-tagged
Thermo Scientific HisPur Nickel-NTA Cartridges protein purification.
Delivers the highest yield of His-tagged protein with a
reuseable resin. Highlights:
• Obtain more than 10mg of pure His-tagged protein per milliliter
Highlights: of resin without optimizing imidazole washing conditions
• Bind up to 60mg of 6xHis-tagged protein per milliliter of resin • Cobalt-chelate coordination core binds fewer host protein con-
• Purify proteins using native or denaturing conditions taminants, resulting in lower background than nickel resins
• Use with Pierce Cell Lysis Reagents and a variety of buffer additives • No metal contamination in eluted histidine-tagged protein sample
• Reuse cartridges several times • Purify proteins under native or denaturing conditions; compatible
with Pierce Cell Lysis Reagents and a variety of buffer additives
4000
M L FT W E
• Reuse cartridges several times
3500
3000 FT W E 6000
2500 Flow-through Wash Elution
5000
2000
A280

1500 4000
1000

A280
500 3000

0
2000
0 20 40 60 80 100 120 140mL

1000
Purification of 6xHis-GFP from E. coli lysate using a Thermo Scientific HisPur
Nickel-NTA Cartridge. Bacterial lysate (130mg total protein) containing over- 0
expressed 6xHis-GFP (green fluorescent protein) was diluted 1:1 with equilibra- 0 5 10 15 20 25mL
tion buffer and applied to a HisPur® Ni-NTA Chromatography Cartridge at a flow
rate of 1mL/min. The cartridge was washed with PBS, 68mM imidazole until
the baseline absorbance was reached. The 6xHis-GFP was eluted with PBS, M S Flow-through (FT) Wash Elution
300mM imidazole. Left panel: 6xHis-GFP elution was monitored at 280nm (black
line) and 485nm (red line; GFP-specific). Right panel: Selected fractions were
analyzed by SDS-PAGE. Gel lanes were normalized to equivalent volume.
M = MW marker, L = lysate load, FT = flow-through and E = elution.

Ordering Information

Product # Description Pkg. Size

90098 HisPur Ni-NTA 5 cartridges
Chromatography Cartridges, 1mL
Formulation: 1mL resin cartridges with Purification of 6xHis-GFP from E. coli lysate using a Thermo Scientific HisPur
LC and Luer-Lok fittings.
Sufficient for: Binding up to 60mg of His-tagged
Cobalt Cartridge. His-tagged green fluorescent protein (GFP) was extracted
protein per cartridge. from E. coli using Thermo Scientific B-PER Bacterial Protein Extraction
Reagent in Phosphate Buffer (Product # 78266) containing Thermo Scientific
90099 HisPur Ni-NTA 2 cartridges Halt Protease Inhibitor Cocktail, EDTA-Free (Product # 78415). The lysate
Chromatography Cartridges, 5mL
Formulation: 5mL resin cartridges with
was diluted 1:1 with equilibration/wash buffer (50mM sodium phosphate,
LC and Luer-Lok fittings. 300mM sodium chloride, 10mM imidazole, pH 7.4) and applied to a HisPur
Sufficient for: Binding up to 300mg of His-tagged Cobalt Chromatography Cartridge at a flow rate of 0.3mL/min. The cartridge
protein per cartridge. was washed with equilibration/wash buffer until the baseline absorbance at
280nm was reached. His-tagged GFP was eluted (50mM sodium phosphate,
300mM sodium chloride, 150mM imidazole; pH 7.4) and selected fractions
were analyzed by SDS-PAGE and Thermo Scientific GelCode Blue Stain
Reagent (Product # 24592). M = MW Marker; S = non-fractionated lysate;
FT = flow-through.

For more information, or to download product instructions, visit www.thermoscientific.com/pierce

2
 mAU
Ordering Information
Sample Wash Elution
4000 Application of
Product # Description Pkg. Size 11.8mg pure
GST-fusion protein
90093 HisPur Cobalt 5 cartridges
Chromatography Cartridges, 1mL 3000
Formulation: 1mL resin cartridges with

A 280
LC and Luer-Lok fittings.
Sufficient for: Binding >10mg of His-tagged 2000
protein per cartridge.
90094 HisPur Cobalt 2 cartridges
Chromatography Cartridges, 5mL 1000
Formulation: 5mL resin cartridges with
LC and Luer-Lok fittings.
Sufficient for: Binding >50mg of His-tagged 0
protein per cartridge.
0.0 10.0 20.0 30.0 40.0 50.0 60.0mL

Purification of Pak 1-GST fusion protein on Thermo Scientific

Pierce Glutathione Chromatography Cartridge, 1mL.
Sample: 50mg (10mL) clarified E. coli lysate containing expressed GST-Pak 1,
M 34,000
Binding Buffer: 5mM Tris, 150mM sodium chloride, pH 8.0
GST-tagged Protein FPLC Purification Elution Buffer: 50mM Tris, 150mM sodium chloride, 10mM Glutathione, pH 8.0
Flow Rate: Load at 0.5mL/min, Wash, Elute 1mL/min
Thermo Scientific Pierce Glutathione Agarose Cartridges Instrument: ÄKTApurifier™
Cost-effective and high-performance GSH resins for purification
of recombinant GST fusion proteins. M Load FT Wash Elution

Highlights: 210

• Binds at least 25mg of recombinant GST protein per milliliter

of resin 110
80
• Consistently purifies at least 10mg of GST-tagged protein per
47
milliliter of resin with greater than 90% purity
• Economically priced and can be reused several times without 32
25
reduction in binding capacity and purification performance
• Works well to purify GST-fusion proteins from bacterial lysates 16.5

or use with pre-purified GST-tagged proteins to pull down protein

interactions Purification of Pak 1-GST fusion protein using the Pierce GST Spin Purification
• Validated and effective for use with Pierce Cell Lysis Reagents to Kit (Product # 16106). Pak 1-GST lysate (2.4mg total protein) was applied in
extract and purify from bacterial or mammalian cell cultures Glutathione Binding Buffer to a 0.2mL Pierce Glutathione Spin Column and
eluted with 10mM Glutathione Elution Buffer, pH 8.0. Fractions were resolved
by SDS-PAGE using a 4-20% Tris-Glycine gel. Gel was stained with GelCode®
Blue Stain Reagent (Product #24590). Pierce 3-Color Protein Molecular Weight
Marker Mix (Product # 26691) was used.

Ordering Information

Product # Description Pkg. Size

16109 Pierce Glutathione 5 cartridges
Chromatography Cartridges, 1mL
Formulation: 1mL resin cartridges with
LC and Luer-Lok fittings.
Sufficient for: Binding approx. 10mg of GST-tagged
protein per cartridge.
16110 Pierce Glutathione 2 cartridges
Chromatography Cartridges, 5mL
Formulation: 5mL resin cartridges with
LC and Luer-Lok fittings.
Sufficient for: Binding approx. 50mg of GST-tagged
protein per cartridge.

To order, call 800-874-3723 or 815-968-0747. Outside the United States, contact your local branch office or distributor.
3
 FPLC Cartridges

Antibody FPLC Purification Thermo Scientific Pierce Protein G Agarose Cartridges

Especially suited for purification of monoclonal antibodies from
Thermo Scientific Pierce Protein A Agarose Cartridges mouse and the broadest spectrum of species and IgG subclasses
Ideal for purification of IgG from serum and other fluids. from human, goat and sheep samples.
Highlights: Highlights:
• Binds to a wide range of antibodies – especially good for • Albumin and cell surface binding site have been removed from
purification of rabbit IgG Protein G to provide higher antibody purity
• Less expensive than Protein G agarose • Binds to a wider range of antibodies than Protein A beads

mAU mAU
FT E FT E
3000 3000
M L FT E ML FT Elution
2500 2500

2000
2000

A280
1500
A280

1500
1000
1000 500
500 0

0 0.0 5.0 10.0 15.0 20.0 25.0 30.0 35.0mL

0.0 10.0 20.0 30.0 40.0 50.0mL

mAU
FT E
3000
ML FT Elution
mAU 2500
FT E
3000 M L FT E
2000
A280

2500 1500

2000 1000
A280

1500 500
0
1000
0.0 5.0 10.0 15.0 20.0 25.0 30.0 35.0mL
500
0
0.0 10.0 20.0 30.0 40.0 50.0mL Comparable antibody yield and purity achieved with Thermo Scientific
Pierce Protein G Chromatography Cartridge. Normal human serum (60mg)
Comparable antibody yield and purity achieved with Thermo Scientific was applied in PBS to a 1mL Thermo Scientific Pierce Protein G Cartridge
Pierce Protein A Chromatography Cartridge. Normal human serum (60mg) (top) and a 1mL HiTrap Column (bottom) and eluted with 0.1M glycine, pH 2.8,
was applied in PBS to a 1mL Thermo Scientific Pierce Protein A Cartridge (top) using a flow rate of 1mL/minute. The arrow denotes the start of the low pH
and a HiTrap™ Column (bottom) and eluted with 0.1M glycine, pH 2.8, using a elution. Fractions were separated by SDS-PAGE and the gels were stained
flow rate of 1mL/minute. The arrow denotes the start of the low-pH elution. with Imperial® Protein Stain (Product # 24615). M = MW marker, L = sample
The yield of human IgG was 6.85mg and 6.88mg, respectively. Fractions were load, FT = flow-through and Elution = elution fractions.
separated by SDS-PAGE and the gels were stained with Thermo Scientific
Imperial Protein Stain (Product # 24615). M = MW marker, L = sample load,
FT = flow-through and E = elution. Ordering Information

Product # Description Pkg. Size

Ordering Information 89926 Pierce Protein G 2 cartridges
Chromatography Cartridges, 1mL
Product # Description Pkg. Size Formulation: 1mL resin cartridges with
LC and Luer-Lok Fittings.
89924 Pierce Protein A 2 cartridges Sufficient for: Binding 11 to 15mg human IgG per cartridge.
Chromatography Cartridges, 1mL
Formulation: 1mL resin cartridges with 89927 Pierce Protein G 1 cartridge
LC and Luer-Lok fittings. Chromatography Cartridge, 5mL
Sufficient for: Binding 35mg human IgG per cartridge. Formulation: 5mL resin cartridge with
LC and Luer-Lok Fittings.
89925 Pierce Protein A 1 cartridge Sufficient for: Binding 55 to 75mg human IgG per cartridge.
Chromatography Cartridge, 5mL
Formulation: 5mL resin cartridge with
LC and Luer-Lok fittings.
Sufficient for: Binding 175mg human IgG per cartridge.

For more information, or to download product instructions, visit www.thermoscientific.com/pierce

4
Thermo Scientific Pierce Protein A/G Agarose Cartridges Thermo Scientific Pierce Protein L Agarose Cartridges
Genetically-engineered protein that combines the IgG binding Great for purifying ScFv or Fab fragments and monoclonal
domains of both Protein A and Protein G. antibodies containing kappa light chains.

Highlights: Highlights:
• Single support provides all benefits of Protein A and Protein G • Binds kappa light chains from a wide range of species without
• Binds a wider range of antibodies than Protein A and interfering with antigen-binding sites†
Protein G beads • Binds to all classes of Ig (e.g., IgG, IgM, IgA, IgE and IgD)†
• Binds single-chain variable fragments (ScFv)†
A) B) • Does not bind bovine, goat or sheep Igs
mAU
4000
M S FT E
• Does not bind to bovine antibodies, making it ideal for purification
3500
of mouse IgG† from cell culture supplemented with bovine serum
3000 kDa †Note: Lambda light chains and some kappa light chains will not bind. Binding will only
210
2500
A280

occur if the appropriate kappa light chains are present.

2000 Pure 110
Rabbit
1500 IgG 80
47 Rabbit
1000 IgG A) Sample
B)
500 32 Application
mAU M L Flow-through Wash Eluate
0 A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A12 B11 B9 B8 B7 B6 B5 B4 B3 B2 B1 C1 C2 C3 C4
25 350
kDa
0.0 10.0 20.0 30.0 40.0mL 300 210
250 IgA IgA
110
Thermo Scientific Pierce Protein A/G Chromatography Cartridges are effec- A280200
80
heavy
150
chain
tive for affinity purification of immunoglobins from serum. A) 2mL of rabbit 100
47
kappa light
serum was applied to the cartridge and the resulting chromatogram recorded. 50
32
25
chain
16.5
B) Fractions were analyzed by SDS-PAGE on a 4-20% Tris-Glycine gel stained 0 A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A12 B11 B9 B8 B7 B6 B5 B4 B3 B2 B1 C1 C2 C3 C4

with Imperial Protein Stain (Product # 24615). M = Marker proteins, S = Sample 0.0 10.0 20.0 30.0mL

applied, FT = Flow-through during sample load and wash, E = Eluted rabbit IgG.
The arrow indicates the location of the isolated IgG. Thermo Scientific Pierce Protein L Chromatography Cartridges isolate and purify
immunoglobulin classes IgG, IgM, IgA, IgE and IgD via their kappa light chains.
A) Human IgA Serum (2mg) was applied in 100mM sodium phosphate, 150mM
sodium chloride, pH 7.2 to a 1mL Pierce Protein L Chromatography Cartridge
Ordering Information and purified at a flow rate of 1mL/minute. Target was eluted in 0.1M glycine,
pH 2.8. B) Fractions were analyzed by SDS-PAGE on a 4-20% Tris-Glycine gel
Product # Description Pkg. Size stained with GelCode® Blue Stain Reagent (Product # 24590).
89930 Pierce Protein A/G Chromatography 2 cartridges
Cartridges, 1mL Ordering Information
Formulation: 1mL resin cartridges with
LC and Luer-Lok Fittings.
Sufficient for: Binding 7mg human IgG per cartridge. Product # Description Pkg. Size
89931 Pierce Protein A/G Chromatography 1 cartridge 89928 Pierce Protein L 2 cartridges
Cartridge, 5mL Chromatography Cartridges, 1mL
Formulation: 5mL resin cartridge with Formulation: 1mL resin cartridges with
LC and Luer-Lok Fittings. LC and Luer-Lok fittings.
Sufficient for: Binding 35mg human IgG per cartridge. Sufficient for: Binding 5 to 10mg human IgG
per cartridge.
89929 Pierce Protein L 1 cartridge
Chromatography Cartridge, 5mL
Formulation: 5mL resin cartridge with
LC and Luer-Lok Fittings.
Sufficient for: Binding 25 to 50mg human IgG
per cartridge.

To order, call 800-874-3723 or 815-968-0747. Outside the United States, contact your local branch office or distributor.
5
 FPLC Cartridges

Thermo Scientific Melon Gel IgG Purification Resins Phosphoprotein FPLC Purification
Easily purify IgG from serum in 15 minutes.
Thermo Scientific Pierce Phosphoprotein Enrichment Kit
Highlights: Purify and isolate phosphoproteins for analysis by Western blotting
• No tedious binding, washing and multiple elution steps or mass spectrometry.
• Purifies antibodies from serum four to six times faster than
Highlights:
Protein A or G methods
• Low nonspecific protein contamination from complex biological
• Recover antibodies from many species with >80% purity
samples, such as cell culture lysate and mouse tissue extract
• No harsh elution conditions means antibodies retain more activity
• Easy spin format enables enrichment of phosphorylated proteins
• Reusable support in less than 2 hours
mAU • Achieves higher yields than other commercially available kits
R
4000
FT R A)
M L 1 2 3 4 1 2 mAU
3000 Non-phosphorylated Concentrated
1200
A280

Proteins Phosphorylated
Flow-Through Proteins In Eluate
2000 1000
FT
800

A280
1000
600

0 400

0.0 10.0 20.0mL 200

Effective reverse-affinity purification of antibody with Thermo Scientific Melon 0

0.0 10.0 20.0 30.0 40.0 50.0 60.0 70.0mL
Gel Cartridges. Normal human serum (1mL) was applied to a 1mL Melon™ Gel
Cartridge at a flow rate of 1mL/minute. Protein contaminants bind to the resin B) Cytochrome-C (12KD)
while the antibodies flow through (FT). Volume of recovered antibody = 4 to Non-phosphorylated protein Phosho-p42/44 MAPK
5mL. Regeneration (R) solution strips the bound protein contaminants so that
the cartridge can be reused multiple times. The purity of the isolated antibody
was evaluated by SDS-PAGE (right panel) and stained with Thermo Scientific
GelCode Blue Stain Reagent (Product # 24590). M = MW Marker, L = sample
loaded, FT = flow-through and R = regeneration fractions. 25µg 10µg FT El 25µg 10µg FT El
TCL TCL

Panel A. Enrichment of phosphorylated proteins from K562 cell lysate,

Ordering Information 4mg (10mL), processed using a Thermo Scientific Pierce Phosphoprotein
Enrichment Chromatography Cartridge, 1mL. Binding/Wash Buffer: 50mM
Product # Description Pkg. Size MES 2-(N-morpholino) ethanesulfonic acid, monohydrate), 250mM sodium
89932 Melon Gel Chromatography Cartridges, 1mL 2 cartridges chloride, 25mM adipic acid, 0.25% CHAPS; pH 5.0; Elution Buffer: 100mM
Formulation: 1mL resin cartridges with sodium phosphate, 500mM sodium chloride, 0.25% CHAPS; pH 7.5; Flow rate
LC and Luer-Lok Fittings. during sample application 0.3mL/min; 1mL/min during wash and elution.
Sufficient for: IgG purification from 1 to 2mL
Panel B. Thermo Scientific Pierce Phosphoprotein Enrichment
serum per cartridge.
Chromatography Cartridge, 1mL, provides high specificity for the enrichment
89933 Melon Gel Chromatography Cartridge, 5mL 1 cartridge of phosphoproteins from complex biological samples. Concentrated Flow-
Formulation: 5mL resin cartridge with through and Elution fractions were resolved on SDS-PAGE. Gel lanes were
LC and Luer-Lok Fittings.
normalized by protein concentration, 10µg/lane. Western blot analysis was
Sufficient for: IgG purification from 5 to 10mL
serum per cartridge. performed using phospho-specific antibodies. Cytochrome C is a negative
control for nonspecific binding of non-phosphorylated proteins. High specific-
ity of binding affinity for the phosphorylated target was demonstrated by the
presence of Phospho-p42/44 MAPK in the Eluate and absence, thereof, in the
Flow-through.

Ordering Information

Product # Description Pkg. Size

87743 Pierce Phosphoprotein Enrichment 2 cartridges
Chromatography Cartridges, 1mL
Formulation: 1mL resin cartridges with
LC and Luer-Lok fittings.
Sufficient for: Purifying samples containing 4mg total
protein (400µg phosphoprotein) per cartridge.
87744 Pierce Phosphoprotein Enrichment 1 cartridge
Chromatography Cartridge, 5mL
Formulation: 5mL resin cartridge with
LC and Luer-Lok fittings.
Sufficient for: Purifying samples containing 20mg total
protein (2mg phosphoprotein) per cartridge.

For more information, or to download product instructions, visit www.thermoscientific.com/pierce

6
Biotinylated Protein FPLC Purification Cartridge Biotinylated
Supplier Size BSA Bound
Thermo Scientific Streptavidin Agarose and 1mL 12.9mg
Pierce High Capacity Streptavidin
Neutravidin Agarose Cartridges Chromatography Cartridge 5mL 75.9mg
Resins with high biotin affinity and low nonspecific binding.
1mL 10.7mg
Highlights: GE HiTrap Streptavidin HP (Not offered
5mL
• Exhibit high binding affinity towards the vitamin Biotin (vitamin H) in 5mL size)
• Captures biotinylated proteins 1mL 12.8mg
Pierce High Capacity NeutrAvidin
• Derivatives of the protein avidin from chicken egg whites that Chromatography Cartridge 5mL 70mg
differ by their source and glycosylation level
Binding capacity of Thermo Scientific High Capacity Streptavidin
Pierce High Capacity Streptavidin Chromatography Cartridge Chromatography Cartridges is comparable to that of HiTrap. Columns were
mAU overloaded with Biotinylated BSA and purified per manufacturer's instructions.
3500 Binding capacity was determined using the Thermo Scientific Pierce BCA
Yield
3000 655µg
Protein Assay Kit (Product # 23225).
2500
Target Note: Capacity for the avidin resins was determined indirectly by subtracting the
unbound biotinylated BSA present in the flow-through fractions from the total amount
2000 applied to the column.
1500
Comparison of biotin-binding proteins.
1000

500 Biotin-Binding Protein

 
0 Avidin Streptavidin NeutrAvidin
0.0 20.0 40.0 60.0 80.0mL
Molecular Weight 67K 53K 60K
GE HiTrap Streptavidin HP Biotin-binding Sites 4 4 4
mAU
Isoelectric Point (pI) 10 6.8-7.5 6.3
3000 Yield
469µg Specificity Low High Highest
2500 Target
Affinity for Biotin (Kd) 10 M
-15
10 M
-15
10-15M
2000

1500 Nonspecific Binding High Low Lowest

1000

500
Ordering Information
0
Product # Description Pkg. Size
0.0 20.0 40.0 60.0 80.0mL
87739 High Capacity Streptavidin Chromatography 2 cartridges
Cartridges, 1mL
Pierce High Capacity NeutrAvidin Chromatography Cartridge
mAU
Formulation: 1mL crosslinked 6% beaded agarose
cartridges with LC and Luer-Lok fittings.
3000 Yield Sufficient for: Binding >100µg biotin per cartridge
388µg (>10mg biotinylated BSA per cartridge).
2500 Target
87740 High Capacity Streptavidin Chromatography 1 cartridge
2000 Cartridge, 5mL
Formulation: 5mL crosslinked 6% beaded agarose
1500
cartridge with LC and Luer-Lok fittings.
1000 Sufficient for: Binding >500µg biotin per cartridge
(>50mg biotinylated BSA per cartridge).
500
87741 High Capacity NeutrAvidin Chromatography 2 cartridges
0 Cartridges, 1mL
0.0 20.0 40.0 60.0 80.0mL Formulation: 1mL crosslinked 6% beaded agarose
cartridges with LC and Luer-Lok fittings.
Thermo Scientific High Capacity Streptavidin Chromatography Cartridge Sufficient for: Binding >75µg biotin per cartridge
achieves comparable yield and performance in binding of small biotinylated (>8mg biotinylated BSA per cartridge).
molecule, Biotin 4 Nitrophenyl ester (BpNPE), as compared to HiTrap. 87742 High Capacity NeutrAvidin Chromatography 1 cartridge
Sample: 4.8mg BpNPE (in 24mL) Cartridge, 5mL
Formulation: 5mL crosslinked 6% beaded agarose
Column Size: 1mL cartridge with LC and Luer-Lok fittings.
Binding Buffer: 0.5M Sodium Acetate, pH 5.0 Sufficient for: Binding >375µg biotin per cartridge
Elution Buffer: 0.5M NaOH Streptavidin cartridges; 0.1M NaOH NeutrAvidin (>40mg biotinylated BSA per cartridge).
cartridge
Flow Rate: Sample Application 0.3mL/minute; Wash and Elution 1mL/minute
Monitored 2 wavelengths: Flow through/ Wash in Na Acetate A 270nm (red);
Elution in NaOH A 410nm (pink)
Instrument: ÄKTApurifier™
Yield determined by optical density at A 410nm (Extinction Coefficient for
BpNPE 18.3 A410nm)

To order, call 800-874-3723 or 815-968-0747. Outside the United States, contact your local branch office or distributor.
7
 FPLC Cartridges

Protein Desalting Ordering Information

Thermo Scientific Zeba Desalting Chromatography Cartridges Product # Description Pkg. Size
The best protein desalting resin in cartridge format. 89934 Zeba Desalting Chromatography Cartridges, 5 cartridges
7K MWCO, 1mL
Highlights: Formulation: 1mL resin cartridges with
• Exceptional desalting and protein-recovery characteristics LC and Luer-Lok fittings.
Sufficient for: Desalting 50 to 250µL samples per use.
compared to other commercially available resins
89935 Zeba Desalting Chromatography Cartridges, 5 cartridges
• Successful with very dilute (25µg/mL) protein samples 7K MWCO, 5mL
• Greater than 95% retention (removal) of salts and other Formulation: 5mL resin cartridges with
LC and Luer-Lok fittings.
small molecules and good recovery of proteins and other Sufficient for: Desalting 100 to 1500µL samples per use.
macromolecules
Thermo Scientific Pierce Chromatography Cartridge
mAU

600
Conductivity (brown)

500
A280 (blue)

400

300

200

100

0
0.0 2.0 4.0 6.0 8.0 10.0mL

Supplier G Chromatography Cartridge

mAU
700

600
Conductivity (brown)

500
A280 (blue)

400

300

200

100

0.0 2.0 4.0 6.0 8.0 10.0mL

Supplier B Chromatography Cartridge

mAU

600
Conductivity (brown)

500

400
A280 (blue)

300

200

100

0.0 2.0 4.0 6.0 8.0 10.0mL

Efficient salt removal and protein recovery with desalting chromatography

cartridge. Bovine serum albumin (1mg) in 1M NaCl was applied to 5mL Thermo
Scientific Zeba Desalting Cartridge (top) at a flow rate of 5mL/minute. Cartridge
profile shows isocratic elution of BSA (blue) and NaCl detected by conductivity
(brown). Greater than 95% of the BSA was recovered and more than 95% of the
salt was removed. Results were comparable to those obtained with desalting
cartridges from other suppliers (results for the Zeba Cartridge were essentially
identical to that obtained with more expensive GE Healthcare and Bio-Rad
Products (suppliers G and B, respectively).

For more information, or to download product instructions, visit www.thermoscientific.com/pierce

8
 Protein Purification Antibody Production and
Technical Handbook Purification Technical Handbook
This 80-page handbook provides This 78-page handbook helps you
protocols and technical and product choose the best methods to produce,
Thermo Scientific Pierce Protein
® information to help maximize results purify, fragment and label antibodies.
Purification Technical Handbook
O
for protein purification. It also Topics include basic immunology,
includes background and trouble carrier proteins, adjuvants, antibody
O
H H2N
Version 2
Agarose
Bead

O N N +
O H2N Bead
O O NH2
NH2

shooting advice for covalent purification methods, antibody frag-

coupling of affinity ligands to mentation with proteases, and label-
chromatography supports, ing antibodies with a variety of tags
avidin:biotin-binding, affinity (e.g., biotin, fluorophores, enzymes,
purification of antibodies, IP and iodine) for purification or detection.
co-IP, affinity procedures for
contaminant removal, and related
procedures.

Ordering Information Dialysis, Desalting and

2K MWCO Membrane Products
2K MWCO – Slide-A-Lyzer MINI Dialysis Units

Product # Description Capacity

U.S.
Pkg. Size Price
30 ml Capacity Slide-A-Lyzer Dialysis Cassettes

Product # Description
87725 Slide-A-Lyzer G2
Capacity
U.S.
Pkg. Size Price
$ 93
12 ml Capacity Slide-A-Lyzer Dialysis Cassettes

Product # Description
66810 Slide-A-Lyzer
Capacity
3-12 ml 8/pkg.
U.S.
Pkg. Size Price
$105
Detergent Removal
Technical Handbook
69580 Slide-A-Lyzer MINI 10-100 μl 50/pkg. $149 30 ml 6/pkg.
Dialysis Unit Kit Dialysis Cassette Dialysis Cassette
69553 Slide-A-Lyzer MINI 10-100 μl 250/pkg. $457 66130 Slide-A-Lyzer 12-30 ml 6/pkg. $ 84 66811 Slide-A-Lyzer 3-12 ml 5 x 8/pkg. $485
Dialysis Units Dialysis Cassette Dialysis Cassette
66807 Slide-A-Lyzer 3-12 ml Kit $130
0.5 ml Capacity Slide-A-Lyzer Dialysis Cassettes 70 ml Capacity Slide-A-Lyzer G2 Dialysis Cassettes Dialysis Cassette Kit
U.S. U.S.
Product # Description Capacity Pkg. Size Price Product # Description Capacity Pkg. Size Price 15 ml Capacity Slide-A-Lyzer G2 Dialysis Cassette

Mass Spec Sample

87717 Slide-A-Lyzer G2 0.1-0.5 ml 10/pkg. $ 91 87726 Slide-A-Lyzer G2 70 ml 6/pkg. $145 U.S.
Dialysis Cassette Dialysis Cassette Product # Description Capacity Pkg. Size Price
66205 Slide-A-Lyzer 0.2-0.5 ml 10/pkg. $ 81 87731 Slide-A-Lyzer G2 15 ml 8/pkg. $117
Dialysis Cassette Dialysis Cassette

3 ml Capacity Slide-A-Lyzer Dialysis Cassettes 7K MWCO Membrane Products

Dialysis is a separation technique

30 ml Capacity Slide-A-Lyzer Dialysis Cassettes
U.S. 7K MWCO – Slide-A-Lyzer MINI Dialysis Units
U.S.
Product # Description Capacity Pkg. Size Price U.S. Product # Description Capacity Pkg. Size Price
87718 Slide-A-Lyzer G2 0.5-3 ml 10/pkg. $ 91 Product # Description Capacity Pkg. Size Price
66830 Slide-A-Lyzer 12-30 ml 6/pkg. $ 84
Dialysis Cassette 69560 Slide-A-Lyzer MINI 10-100 μl 50/pkg. $149

Preparation Handbook
Dialysis Cassette
66203 Slide-A-Lyzer 0.5-3 ml 10/pkg. $ 81 Dialysis Unit
87732 Slide-A-Lyzer G2 30 ml 6/pkg. $ 93
Dialysis Cassette 69562 Slide-A-Lyzer MINI 10-100 μl 250/pkg $457 Dialysis Cassette
Dialysis Unit
12 ml Capacity Slide-A-Lyzer Dialysis Cassettes 70 ml Capacity Slide-A-Lyzer G2 Dialysis Cassette

that gained popularity in life

U.S. 0.5 ml Capacity Slide-A-Lyzer Dialysis Cassettes U.S.
Product # Description Capacity Pkg. Size Price Product # Description Capacity Pkg. Size Price
U.S.
66212 Slide-A-Lyzer 3-12 ml 8/pkg. $105 Product # Description Capacity Pkg. Size Price 87733 Slide-A-Lyzer G2 $145
70 ml 6/pkg.
Dialysis Cassette Dialysis Cassette
87727 Slide-A-Lyzer G2 0.1-0.5 ml 10/pkg. $ 91

Thermo Scientific Pierce High-Performance

15 ml Capacity Slide-A-Lyzer G2 Dialysis Cassettes Dialysis Cassette
66373 Slide-A-Lyzer 0.1-0.5 ml 10/pkg. $ 81 l Irradiated 10K MWCO Membrane
U.S.
Product # Description Capacity Pkg. Size Price Dialysis Cassette U.S.
Product # Description Capacity Pkg. Size Price

Dialysis, Desalting and Detergent Removal

66375 Slide-A-Lyzer 0.1-0.5 ml Kit $ 99

The improved version of the

87719 Slide-A-Lyzer G2 15 ml 8/pkg. $117
Dialysis Cassette Kit 66454 Slide-A-Lyzer 0.1-0.5 ml 10/pkg. $109

science laboratories during the

Dialysis Cassette
Dialysis Cassette
30 ml Capacity Slide-A-Lyzer Dialysis Cassettes 66455 Slide-A-Lyzer 0.5-3 ml 10/pkg. $119

Technical Handbook
3 ml Capacity Slide-A-Lyzer Dialysis Cassettes Dialysis Cassette
U.S. U.S.
Product # Description Capacity Pkg. Size Price Product # Description Capacity Pkg. Size Price 66453 Slide-A-Lyzer 3-12 ml 8/pkg. $110
87720 Slide-A-Lyzer G2 $ 93 Dialysis Cassette
30 ml 6/pkg. 87728 Slide-A-Lyzer G2 0.5-3 ml 10/pkg. $ 91
Dialysis Cassette 66456 Slide-A-Lyzer 12-30 ml 6/pkg. $ 88
66230 Slide-A-Lyzer 12-30 ml 6/pkg. $ 84 66370
Dialysis Cassette
Slide-A-Lyzer 0.5-3 ml 10/pkg. $ 81
Dialysis Cassette Featuring Thermo Scientific Slide-A-Lyzer Dialysis Cassettes

Mass Spec Sample Prep provides

Dialysis Cassette Dialysis Cassette

1950s. Research papers of that era

66372 Slide-A-Lyzer 0.5-3 ml Kit $113
70 ml Capacity Slide-A-Lyzer G2 Dialysis Cassettes
Dialysis Cassette Kit 20K MWCO Membrane Products Contact Information
U.S. 0.5 ml Capacity Slide-A-Lyzer Dialysis Cassettes

Version 2
Product # Description Capacity Pkg. Size Price
U.S. Belgium and Europe,
87721 Slide-A-Lyzer G2 70 ml 6/pkg. $145 12 ml Capacity Slide-A-Lyzer Dialysis Cassettes Product # Description Capacity Pkg. Size Price
Dialysis Cassette U.S. the Middle East
87734 Slide-A-Lyzer G2 0.1-0.5 ml 10/pkg. $ 91 and Africa Distributors
Product # Description Capacity Pkg. Size Price
Dialysis Cassette
66710 Slide-A-Lyzer 3-12 ml 8/pkg. $105 Tel: +32 53 85 71 84
66005 Slide-A-Lyzer 0.1-0.5 ml 10/pkg. $ 81

background, helpful hints and

Dialysis Cassette
3.5K MWCO Membrane Products Dialysis Cassette

described dialysis as a new, cutting-

66707 Slide-A-Lyzer 3-12 ml Kit $130 France
3.5K MWCO – Slide-A-Lyzer MINI Dialysis Units Dialysis Cassette Kit Tel: 0 800 50 82 15
U.S. 3 ml Capacity Slide-A-Lyzer Dialysis Cassettes
Product # Description Capacity Pkg. Size Price U.S. The Netherlands
69558 Slide-A-Lyzer MINI 10-100 μl Kit $ 66 Product # Description Capacity Pkg. Size Price
10K MWCO Membrane Products Tel: 076 50 31 880

Mass Spec Sample Preparation Handbook

Dialysis Unit Kit 87735 Slide-A-Lyzer G2 0.5-3 ml 10/pkg. $ 91
69550 Slide-A-Lyzer MINI 10-100 μl 50/pkg. $149 10K MWCO – Slide-A-Lyzer MINI Dialysis Units Dialysis Cassette
Dialysis Units U.S. Germany
66003 Slide-A-Lyzer 0.5-3 ml 10/pkg. $ 81
Product # Description Capacity Pkg. Size Price Tel: 0228 9125650

troubleshooting advice for cell

69552 Slide-A-Lyzer MINI 10-100 μl 250/pkg. $457
Featuring Thermo Scientific Pierce Products to extract, digest, Dialysis Cassette

edge tool that scientists could use

Dialysis Units 69574 Slide-A-Lyzer MINI 10-100 μl 10/pkg. $ 59
Dialysis Unit United Kingdom
enrich, clean up and quantify proteins and peptides 0.5 ml Capacity Slide-A-Lyzer Dialysis Cassettes Plus Microtubes 12 ml Capacity Slide-A-Lyzer Dialysis Cassettes Tel: 0800 252 185
U.S. 69570 Slide-A-Lyzer MINI 10-100 μl 50/pkg. $149 U.S.
Product # Description Capacity Pkg. Size Price Dialysis Unit Product # Description Capacity Pkg. Size Price
Switzerland
87722 Slide-A-Lyzer G2 0.1-0.5 ml 10/pkg. $ 91 69572 Slide-A-Lyzer MINI 10-100 μl 250/pkg. $457 66012 Slide-A-Lyzer 3-12 ml 8/pkg. $105
Dialysis Cassette Tel: 0800 56 31 40
Dialysis Cassette Dialysis Unit
66333 Slide-A-Lyzer 0.1-0.5 ml 10/pkg. $ 81 69576 Slide-A-Lyzer MINI 10-100 μl Kit/10 units $ 66 Email: [email protected]

lysis, sample prep, detection, mass

Dialysis Cassette Dialysis Unit 15 ml Capacity Slide-A-Lyzer G2 Dialysis Cassette www.thermo.com/perbio

Version 2
to unravel complex mixtures of
66335 Slide-A-Lyzer 0.1-0.5 ml Kit $ 99 Plus Float U.S.
Dialysis Cassette Kit Product # Description Capacity Pkg. Size Price
0.5 ml Capacity Slide-A-Lyzer Dialysis Cassettes United States
87736 Slide-A-Lyzer G2 15 ml 8/pkg. $117
3 ml Capacity Slide-A-Lyzer Dialysis Cassettes U.S. Dialysis Cassette
Tel: 815-968-0747 or 800-874-3723
U.S. Product # Description Capacity Pkg. Size Price Customer Assistance E-mail:
Product # Description Capacity Pkg. Size Price 87729 Slide-A-Lyzer G2 0.1-0.5 ml 10/pkg. $ 91 [email protected]
87723 Slide-A-Lyzer G2 0.5-3 ml 10/pkg. $ 91 30 ml Capacity Slide-A-Lyzer Dialysis Cassette
Dialysis Cassette www.thermo.com
1601822 12/09 Printed in U.S.

Dialysis Cassette 66383 Slide-A-Lyzer 0.1-0.5 ml 10/pkg. $ 81 U.S.

Product # Description Capacity Pkg. Size Price

spec sample prep and downstream

66330 Slide-A-Lyzer 0.5-3 ml 10/pkg. $ 81 Dialysis Cassette

biomacromolecules. Many of the

Dialysis Cassette 87737 Slide-A-Lyzer G2 30 ml 6/pkg. $ 93
66384 Slide-A-Lyzer 0.1-0.5 ml 5 x 10/pkg. $380
66332 Slide-A-Lyzer 0.5-3 ml Kit $113 Dialysis Cassette
Dialysis Cassette
Dialysis Cassette Kit 66030 Slide-A-Lyzer 12-30 ml 6/pkg. $ 84
66385 Slide-A-Lyzer 0.1-0.5 ml Kit $ 99 © 2009 Thermo Fisher Scientific Inc. All rights reserved.
Dialysis Cassette
Dialysis Cassette Kit These products are supplied for laboratory or manufacturing
12 ml Capacity Slide-A-Lyzer Dialysis Cassettes
U.S. applications only. Unless indicated otherwise on the inside
Product # Description Capacity Pkg. Size Price
3 ml Capacity Slide-A-Lyzer Dialysis Cassettes 70 ml Capacity Slide-A-Lyzer G2 Dialysis Cassette front cover, all trademarks are property of Thermo Fisher
U.S. U.S. Scientific Inc. and its subsidiaries.
66110 Slide-A-Lyzer 3-12 ml 8/pkg. $105 Product # Description Capacity Pkg. Size Price Product # Description Capacity Pkg. Size Price

applications. The handbook

Dialysis Cassette
87730 Slide-A-Lyzer G2 0.5-3 ml 10/pkg. $ 91

dialysis theories established at

66107 Slide-A-Lyzer 3-12 ml Kit $130 87738 Slide-A-Lyzer G2 70 ml 6/pkg. $145
Dialysis Cassette Dialysis Cassette
Dialysis Cassette Kit
66380 Slide-A-Lyzer 0.5-3 ml 10/pkg. $ 81
15 ml Capacity Slide-A-Lyzer G2 Dialysis Cassettes Dialysis Cassette
U.S. 66381 Slide-A-Lyzer 0.5-3 ml 5 x 10/pkg. $380
Product # Description Capacity Pkg. Size Price Dialysis Cassette
87724 Slide-A-Lyzer G2 15 ml 8/pkg. $117 66382 Slide-A-Lyzer 0.5-3 ml Kit $113
Dialysis Cassette Dialysis Cassette Kit

features new products for protein that time are the cornerstones for
concentration, purification and contemporary products featured in
enrichment, plus the latest labeling this brochure. There are, however,
techniques including SILAC, TMT, two major differences between the
cysTMT and HeavyPeptides dialysis tools of yesterday and today
reagents. The book also includes a – preparation time and the amount
section on Thermo Scientific Mass of sample loss due to leaks. Early
Spectrometry Instrumentation and laboratory dialysis methods involved
Software. Everything you need to dedicating a significant amount
extract, digest, enrich, clean up and of time to membrane preparation;
quantify proteins and peptides in Thermo Scientific Pierce Dialysis
one volume. Products are essentially ready to use
and resist sample leakage.

To order, call 800-874-3723 or 815-968-0747. Outside the United States, contact your local branch office or distributor.
9
 Life Science Research
Contact Information

Belgium and Europe,

the Middle East
and Africa Distributors
Tel: +32 53 85 71 84

France
Tel: 0 800 50 82 15

The Netherlands
Tel: 076 50 31 880

Germany
Tel: 0228 9125650

United Kingdom
Tel: 0800 252 185

Switzerland
Tel: 0800 56 31 40

Email: [email protected]
www.thermoscientific.com/perbio

United States
Tel: 815-968-0747 or 800-874-3723
Customer Assistance E-mail:
[email protected]
1602101 12/10 Printed in the U.S.

www.thermoscientific.com

© 2010 Thermo Fisher Scientific Inc. All rights reserved.

These products are supplied for laboratory or manufacturing
applications only. Luer-Lok® is a trademark of Becton,
Dickinson and Company. ÄKTA® and HiTrap™ are trademarks of
GE Healthcare. Unless indicated otherwise, all trademarks are
property of Thermo Fisher Scientific Inc. and its subsidiaries.