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    Beer's Law Lab Questions

    Uploaded by

    Annie Daley
    1. The experiment uses a spectrophotometer to measure the absorbance of solutions containing the complex ion [Fe(SCN)]2+ at a wavelength of 459.0 nm. This wavelength corresponds to the color of the most concentrated solution. 2. The relationship between concentration and absorbance follows Beer's Law, which can be expressed by the equation y = 3890x + 0.02475, relating absorbance (y) to concentration (x). 3. The iron concentration is kept higher than the potassium thiocyanate concentration to prevent the color from being overwhelmed, since the reddish color comes from the iron ion. Different amounts of KSCN are added to dilute the solution

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    Beer's Law Lab Questions

    Uploaded by

    Annie Daley
    169 views2 pages
    1. The experiment uses a spectrophotometer to measure the absorbance of solutions containing the complex ion [Fe(SCN)]2+ at a wavelength of 459.0 nm. This wavelength corresponds to the color of the most concentrated solution. 2. The relationship between concentration and absorbance follows Beer's Law, which can be expressed by the equation y = 3890x + 0.02475, relating absorbance (y) to concentration (x). 3. The iron concentration is kept higher than the potassium thiocyanate concentration to prevent the color from being overwhelmed, since the reddish color comes from the iron ion. Different amounts of KSCN are added to dilute the solution

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    1. The experiment uses a spectrophotometer to measure the absorbance of solutions containing the complex ion [Fe(SCN)]2+ at a wavelength of 459.0 nm. This wavelength corresponds to the color of the most concentrated solution. 2. The relationship between concentration and absorbance follows Beer's Law, which can be expressed by the equation y = 3890x + 0.02475, relating absorbance (y) to concentration (x). 3. The iron concentration is kept higher than the potassium thiocyanate concentration to prevent the color from being overwhelmed, since the reddish color comes from the iron ion. Different amounts of KSCN are added to dilute the solution

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    169 views2 pages

    Beer's Law Lab Questions

    Uploaded by

    Annie Daley
    1. The experiment uses a spectrophotometer to measure the absorbance of solutions containing the complex ion [Fe(SCN)]2+ at a wavelength of 459.0 nm. This wavelength corresponds to the color of the most concentrated solution. 2. The relationship between concentration and absorbance follows Beer's Law, which can be expressed by the equation y = 3890x + 0.02475, relating absorbance (y) to concentration (x). 3. The iron concentration is kept higher than the potassium thiocyanate concentration to prevent the color from being overwhelmed, since the reddish color comes from the iron ion. Different amounts of KSCN are added to dilute the solution

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 2

    Questions:

    1. Why is the experiment run at a wavelength near 445 nm?


    That wavelength is the color that the solution was, so we ran ours at 459.0 nm
    because that was the color of our most concentrated solution. The most
    concentrated solution absorbs the least amount of light, so the spectrometer can
    get an accurate reading of the wavelength reflected.
    2. What is the relationship between concentration and absorbance in Part I of the
    experiment?
    The relationship between concentration and absorbance in part I is Beer’s Law,
    which, when graphed, forms a straight line which can be described by the
    equation 𝑦 = 3890𝑥 + .02475, in which x represents concentration and y
    represents absorbance. So, if you multiply concentration by 3890 and add .02475,
    you’ll end up with the absorbance. Inversely, if you subtract .02475 from the
    absorbance and then divide it by 3890, you’ll end up with the concentration.
    3. Why is the Fe2+ concentration so much higher than the KSCN concentration in
    Part I of the experiment?
    The reddish/orangish color came from the Fe+, so it couldn’t be drowned out by
    too much KSCN to drown it out. The quantity of Fe+ is kept constant and
    different quantities of KSCN added to dilute the color and concentration of the
    solution.
    4. What is the difference between absorbance and % transmission, and what formula
    relates them?
    Transmission is quantity of light reflected, and absorption is the quantity of light
    that is absorbed. Colors that are not absorbed are bounced back and the substance
    appears that/those color/s. If nothing is absorbed, than transmission is 100%. If
    nothing is transmitted, absorption is infinite. Transmission and absorption are
    related by the Beer’s Law formula, A=ebc.
    Conclusion:
    In this experiment, the equilibrium constant for the reaction between potassium
    thiocyanate and iron (III) nitrate was determined by using a spectrophotometer to study a
    series of solutions of known concentrations of [Fe(SCN)]+2 to determine absorbance’s at
    a specific wavelength (459.0nm). Then, a number of trials combined different quantities
    of Fe(NO3)3 and KSCN to form [Fe(SCN0]+2. Using the graph generated from the first
    part of the experiment (concentrations of [Fe(SCN0]+2), the concentration of all species
    in the product [Fe(SCN)]+2 were determined. This lab taught the valuable lesson that light
    can measure concentration, and so give an equilibrium constant, and it taught how to
    select the correct wavelength on which to take the measurements and how to find the
    concentrations from the raw data generated by the spectrophotometer. My group got
    relatively good, but not splendid data, with 11.0% relative uncertainty. We had no outlier
    (as determined by performing a Q test on the lowest and highest values) and came up
    with an average Keq of 357, which is relatively close to both the lowest value (306) and
    the highest (404). It was not particularly precise data, but 11.0% relative uncertainty is
    certainly a promising figure.

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