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    T4 DNA Ligase Buffer For Ion Torrent

    Uploaded by

    Nabilah
    This document is a material safety data sheet (MSDS) for T4 DNA Ligase Buffer. The MSDS provides information on the composition, properties, handling, storage, exposure, and disposal of the buffer. The buffer contains 1.5% DTT and 6% Tris buffered with HCl. It may cause eye irritation but has low flammability and reactivity. Proper handling and storage procedures are outlined to minimize risks when working with the buffer.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    T4 DNA Ligase Buffer For Ion Torrent

    Uploaded by

    Nabilah
    180 views3 pages
    This document is a material safety data sheet (MSDS) for T4 DNA Ligase Buffer. The MSDS provides information on the composition, properties, handling, storage, exposure, and disposal of the buffer. The buffer contains 1.5% DTT and 6% Tris buffered with HCl. It may cause eye irritation but has low flammability and reactivity. Proper handling and storage procedures are outlined to minimize risks when working with the buffer.

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    T4 DNA Ligase Buffer

    Original Title

    T4 DNA Ligase Buffer for Ion Torrent

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    This document is a material safety data sheet (MSDS) for T4 DNA Ligase Buffer. The MSDS provides information on the composition, properties, handling, storage, exposure, and disposal of the buffer. The buffer contains 1.5% DTT and 6% Tris buffered with HCl. It may cause eye irritation but has low flammability and reactivity. Proper handling and storage procedures are outlined to minimize risks when working with the buffer.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    180 views3 pages

    T4 DNA Ligase Buffer For Ion Torrent

    Uploaded by

    Nabilah
    This document is a material safety data sheet (MSDS) for T4 DNA Ligase Buffer. The MSDS provides information on the composition, properties, handling, storage, exposure, and disposal of the buffer. The buffer contains 1.5% DTT and 6% Tris buffered with HCl. It may cause eye irritation but has low flammability and reactivity. Proper handling and storage procedures are outlined to minimize risks when working with the buffer.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 3

    CLONING & MAPPING

    DNA AMPLIFICATION & PCR


    RNA ANALYSIS
    PROTEIN EXPRESSION & ANALYSIS
    GENE EXPRESSION & CELLULAR ANALYSIS

    MATERIAL SAFETY DATA SHEET


    Telephone: (978)927-5054
    Toll free: (800)632-5227
    Fax: (978)921-1350
    NEB# E6296
    New England Biolabs
    240 County Road Email: [email protected]
    Ipswich, MA 01938 Revision Date: 10/01/2013

    SECTION 1CHEMICAL INFORMATION


    Product Name: T4 DNA Ligase Buffer Cas.# None

    SECTION 2COMPOSITION/INFORMATION ON INGREDIENT


    1. DTT 1.5% Cas.# 3483-12-3
    2. Tris buffered with HCl 6% Cas.# 77-86-1
    Note: The CAS number listed above is for Tris. HCl ( < 1%) was added to the Tris to alter the pH level.
    The ingredients listed in this section include only those items that have more than 1% of a component classified as hazardous and 0.1% of a
    component classified as carcinogenic. If you have any questions, please contact [email protected].

    SECTION 3HAZARDOUS IDENTIFICATION


    Emergency Overview: May cause irritation to eyes.
    HMIS and NFPA Ratings: 0 Minimal or None, 1 Slight, 2 Moderate, 3 Serious, and 4 Severe

    Health: 1
    Flammability: 0
    Reactivity: 0

    SECTION 4FIRST AID MEASURES


    Eyes: Flush eyes with copious amounts of water for at least 15 minutes. Assure adequate flushing by separating eyelids. Call a physician.
    Skin: Wash skin with soap and copious amount of water.
    Ingestion: If the person is conscious, wash out mouth with water. Call a physician.
    Inhalation: Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician.

    SECTION 5FIRE FIGHTING MEASURES


    Extinguishing Media: Water spray. Carbon dioxide, dry chemical powder or appropriate foam.
    Special Fire Fighting Procedures: Wear self contained breathing apparatus and protective clothing to prevent contact with skin and eyes.
    Fire and Explosion Hazards: Combustible liquid. Emits toxic fumes under fire conditions.

    Page 1
    SECTION 6ACCIDENTAL RELEASE MEASURES
    Personal Precautions: Avoid breathing or contact with vapors, mist of gas.
    Environmental Precautions: Do not let product enter drains.
    Methods For Cleaning Up: Cover with dry lime, sand, or soda ash. Sweep up and shovel. Place in covered container for disposal.

    SECTION 7HANDLING AND STORAGE


    Handling: Provide appropriate exhaust ventilation.
    Storage: Keep tightly closed in a dry and well ventilated place. Store at 20C .

    SECTION 8EXPOSURE CONTROLS/PPE


    Engineering Controls: Safety shower and eye wash. Mechanical exhaust.
    Personal Protective Equipment:
    Eye Protection: Safety goggles.
    Hand Protection: Compatible resistant gloves.
    Respiratory Protection: None required.
    Hygiene Measure: General practice, wash (hands and skin) thoroughly after handling. Remove and wash contaminated clothing.

    SECTION 9PHYSICAL AND CHEMICAL PROPERTIES


    Physical State: Form: Liquid Color: Clear or colorless Odor: None
    Property Value Temperature or Pressure
    Boiling Point Range: No data available
    Melting Point Range: No data available
    Flash Point: No data available
    Auto Ignition Temp: No data available
    Vapor Pressure: No data available
    Vapor Density: No data available
    Specific Gravity: No data available
    Solubility in Water: Soluble

    SECTION 10STABILITY AND REACTIVITY


    Stability: Stable under recommended storage conditions.
    Materials to Avoid: Strong oxidizing agents.
    Hazardous Decomposition Products: Carbon monoxide, carbon dioxide, nitrogen oxides, hydrogen chlonde gas, carbon oxides and
    sodium oxides.
    Hazardous Polymerization: Will not occur.

    SECTION 11TOXICOLOGICAL INFORMATION


    Acute and Chronic Affects Based On Routes Of Exposure
    Eye Contact: May cause irritation.
    Skin Contact: May cause irritation.
    Ingestion: May cause vomiting, diarrhea, dehydration and congestion in the internal organs.
    Inhalation: Mist may cause irritation of respiratory tract and gastrointestinal tract.
    Chronic Exposure
    Target Organ(s): No data available.
    Aggravation of Pre-existing Conditions: Persons with pre-existing skin disorder or eye problems may be more susceptible to the effects of the
    material.
    NTP: No component of this product present at levels greater than or equal to 0.1% is identified as a known or anticipated carcinogen.
    IARC: No component of this product present at levels greater than or equal to 0.1 % is identified as probable, possible or confirmed human
    carcinogen.
    ACGIH: No component of this product present at levels greater than or equal to 0.1 % is identified as a known or suspected human carcinogen
    or confirmed animal with unknown relevance humans. Page 2
    SECTION 12ECOLOGICAL INFORMATION
    No data available.

    SECTION 13DISPOSAL CONSIDERATIONS


    Dispose of container, unused contents and contaminated packaging in accordance with federal, state and local requirement. Contract with a
    licensed Chemical Waste Disposal Service.

    SECTION 14TRANSPORT INFORMATION


    This product is not dangerous and no special precautions are needed according to DOT, ADR/RID (cross border), IMDG and IATA/ICAO.

    SECTION 15REGULATORY INFORMATION


    OSHA Hazards: None known.
    US Classification and Label Test
    US Statements: Caution. Avoid contact and inhalation.
    United States Regulatory Information:
    Sara Listed: No
    TSCA Inventory Item: Yes
    Canada Regulatory Information
    WHMIS Classification: This product has been classified in accordance with the hazard criteria of the CPR and the MSDS contains all the
    information required by the CPR.
    DSL: Yes
    NDSL: No
    EU Additional Classification
    S: 23 24/25
    Safety Statements: Do not breath vapor. Avoid contact with skin and eyes.

    SECTION 16OTHER INFORMATION


    DISCLAIMER
    The information provided on the MSDS is furnished in good faith and based on our present knowledge. However, this MSDS shall not consti-
    tute a guarantee of any kind. Personnel handling this material must make independent determinations of the suitability and completeness of
    information from all sources to assure proper use and disposal of this material and the safety and health of employees and customers. NEB
    assumes no additional liability or responsibility resulting from the use of, or reliance on this information. This product is for R&D use only.
    Not for drug, household or other uses.
    Questions about the information found on this MSDS should be directed to [email protected].

    Page 3

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