This document provides information on DRBC Agar Base, a selective medium for enumerating yeasts and molds according to ISO 21527. It lists the typical formula, describes the components and their roles, and provides preparation, testing, storage and safety instructions.
This document provides information on DRBC Agar Base, a selective medium for enumerating yeasts and molds according to ISO 21527. It lists the typical formula, describes the components and their roles, and provides preparation, testing, storage and safety instructions.
This document provides information on DRBC Agar Base, a selective medium for enumerating yeasts and molds according to ISO 21527. It lists the typical formula, describes the components and their roles, and provides preparation, testing, storage and safety instructions.
This document provides information on DRBC Agar Base, a selective medium for enumerating yeasts and molds according to ISO 21527. It lists the typical formula, describes the components and their roles, and provides preparation, testing, storage and safety instructions.
Basal medium for the enumeration of yeasts and moulds, according to ISO 21527.
TYPICAL FORMULA (g/l)
Enzymatic Digest of Animal and Plant Tissues 5.0 Glucose 10.0 Potassium Dihydrogenphosphate 1.0 Magnesium Sulfate 0.5 Dichloran (2,6-dichloro-4-nitroanile) 0.002 Rose Bengal 0.025 Agar 15.0 Final pH 5.6 0.2 at 25C
DESCRIPTION DRBC Agar Base (Dichloran Rose Bengal Chloramphenicol) is a selective medium used with supplements for the enumeration of yeasts and moulds, according to ISO 21527.
PRINCIPLE Enzymatic digest of plant and animal tissues provides carbon, nitrogen, vitamins and minerals required for organism growth. Glucose is an energy source. Phosphate is a buffering agent. Magnesium sulfate is a source of divalent cations and sulfate. The antifungal agent, dichloran, is included to reduce colony diameters of spreading fungi. Rose bengal suppresses the growth of bacteria and restricts the size and height of colonies of the more rapidly growing moulds. Inhibition of bacterial growth and restriction of spreading of more-rapidly growing moulds aids in the isolation of slow-growing fungi. Also the low pH serves to prevent the growth of most bacteria. Agar is the solidifying agent. Chloramphenicol is added to the medium to inhibit the growth of bacteria present in environmental and food samples.
PREPARATION Suspend 31.5 g of the powder in 1 liter of purified water. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Add rehydrated content of 2 vials (6 ml) of Chloramphenicol Supplement (ref. 81017). Autoclave at 121C for 15 minutes. Cool to 45-50C. Pour into Petri dishes.
TECHNIQUE Inoculate 0.1 ml of sample and/or appropriate decimal dilutions of sample by streaking over the entire surface of the agar. Incubate plates aerobically at 25C for up to 5 days. Examine for growth of yeasts and moulds daily after 2 days incubation.
INTERPRETATION OF RESULTS Colonies of yeast appear pink due to the uptake of rose bengal. Where identification is necessary, extend incubation until morphological characteristics are more evident and/or perform microscopic examination. Report the results as colony-forming units per gram or milliliter of sample.
STORAGE The powder is very hygroscopic, store the powder at 10-30C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until sings of deterioration or contamination are evident. Store prepared plates at 2-8C away from light.
WARNING AND PRECAUTIONS
The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is not classified as dangerous. it is nevertheless recommended to consult the safety data sheet for its correct use. The product must be used only by properly trained operators.
DISPOSAL OF WASTE Disposal of waste must be carried out according to national and local regulations in force.
REFERENCES 1. ISO 21527: 2008 Microbiology of food and animal feeding stuffs -- Horizontal method for the enumeration of yeasts and moulds 2. King, Hocking and Pitt. 1979. Appl. Environ. Microbiol. 37:959. 3. Beuchat and Cousin. 2001. In Downes and Ito (ed.). Compendium of methods for the microbiological examination of foods, 4th ed. American Public Health Association. Washington, D.C. 4. U.S. Food and Drug Administration. 1995. Bacteriological analytical manual, 8th ed. AOAC International, Gaithersburg, Md. 5. Banks, Board and Paton. 1985. Lett. Appl. Microbiol. 1:7.
LIOFILCHEM S.r.l. Via Scozia, Zona Ind.le - 64026, Roseto degli Abruzzi (TE) - ITALY Tel +39 0858930745 Fax +39 0858930330 Website: www.liofilchem.net E-mail: [email protected] TECHNICAL SHEET TS 610237 Rev.3 / 27.06.2014 Page 2 of 2
PRODUCT SPECIFICATIONS NAME DRBC Agar Base
PRESENTATION Dehydrated medium
STORAGE 10-30C
PACKAGE Ref. Content Packaging 610237 500 g 500 g of powder in plastic bottle 610237 500 g 500 g of powder in plastic bottle
pH OF THE MEDIUM 5.6 0.2
USE DRBC Agar Base (Dichloran Rose Bengal Chloramphenicol) is a selective medium used with supplements for the enumeration of yeasts and moulds, according to ISO 21527
TECHNIQUE Refer to technical sheet of the product
APPEARANCE OF THE MEDIUM
Dehydrated medium Appearance: free-flowing, homogeneous Colour: pink Prepared medium Appearance: very slightly to slightly opalescent Colour: bright pink
SHELFLIFE 4 years
QUALITY CONTROL 1. Control of general characteristics, label and print 2. Sterility control 7 days at 22 1C, in aerobiosis 7 days at 36 1C, in aerobiosis
3. Microbiological control Inoculum for productivity: 10-100 CFU/ml Inoculum for selectivity: 104-105 CFU/ml Incubation conditions: 2-5 days at 25-30C, in aerobiosis
Microorganism Growth Aspergillus niger ATCC 16404 Good Candida albicans ATCC 10231 Good Escherichia coli ATCC 25922 Inhibited
TABLE OF SYMBOLS
Batch Keep away from Fragile, handle
Manufacturer Use by code heat sources with care
Catalogue Temperature Contains sufficient Consult instructions
number limitation for <n> tests for use
LIOFILCHEM S.r.l. Via Scozia, Zona Ind.le - 64026, Roseto degli Abruzzi (TE) - ITALY Tel +39 0858930745 Fax +39 0858930330 Website: www.liofilchem.net E-mail: [email protected]
ISO 6888-1 1999 Microbiology of Food and Animal Feeding Stuffs - Enumeration of Coag-Positive Staphylococci Part 1 Technique Using Baird-Parker Agar Medium
