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    Homework 7 Bme260 s16 Key

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    xryceu
    This homework assignment involves solving several problems related to cell culture growth kinetics: 1) Determine the time for a cell culture to triple in size given an initial population of 100 cells that increases 1.5 times every 12 hours. Also determine the time for a culture of 1 million cells to triple. 2) Calculate the initial cell concentration given concentrations of 400 cells/mL after 3 hours and 2000 cells/mL after 10 hours. 3) Calculate the time needed for an E. coli culture to reach 400 million cells/L given an initial concentration of 10,000 cells/L and information about growth parameters. 4) Analyze data from a batch culture of mold over time to determine the specific growth

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    Homework 7 Bme260 s16 Key

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    xryceu
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    This homework assignment involves solving several problems related to cell culture growth kinetics: 1) Determine the time for a cell culture to triple in size given an initial population of 100 cells that increases 1.5 times every 12 hours. Also determine the time for a culture of 1 million cells to triple. 2) Calculate the initial cell concentration given concentrations of 400 cells/mL after 3 hours and 2000 cells/mL after 10 hours. 3) Calculate the time needed for an E. coli culture to reach 400 million cells/L given an initial concentration of 10,000 cells/L and information about growth parameters. 4) Analyze data from a batch culture of mold over time to determine the specific growth

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    This homework assignment involves solving several problems related to cell culture growth kinetics: 1) Determine the time for a cell culture to triple in size given an initial population of 100 cells that increases 1.5 times every 12 hours. Also determine the time for a culture of 1 million cells to triple. 2) Calculate the initial cell concentration given concentrations of 400 cells/mL after 3 hours and 2000 cells/mL after 10 hours. 3) Calculate the time needed for an E. coli culture to reach 400 million cells/L given an initial concentration of 10,000 cells/L and information about growth parameters. 4) Analyze data from a batch culture of mold over time to determine the specific growth

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    600 views4 pages

    Homework 7 Bme260 s16 Key

    Uploaded by

    xryceu
    This homework assignment involves solving several problems related to cell culture growth kinetics: 1) Determine the time for a cell culture to triple in size given an initial population of 100 cells that increases 1.5 times every 12 hours. Also determine the time for a culture of 1 million cells to triple. 2) Calculate the initial cell concentration given concentrations of 400 cells/mL after 3 hours and 2000 cells/mL after 10 hours. 3) Calculate the time needed for an E. coli culture to reach 400 million cells/L given an initial concentration of 10,000 cells/L and information about growth parameters. 4) Analyze data from a batch culture of mold over time to determine the specific growth

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    Homework 7 BME 260L

    DUE Tues. April 12th


    Name KEY

    1. A cell culture is initially composed of 100 cells. After 12 hours the number of cells is
    1.5 times the number in the initial population.

    a. If the rate of growth is proportional to the number of cells present, determine the
    time necessary for the number of cells to triple.
    b. What is the time required for a culture with 1 x 106 of the same cells to triple?
    Explain your results.
    c. Under what conditions would the answers obtained in part b) be invalid?
    2. For a specific type of cell after 3 hours, the concentration of cells per milliliter of solution
    is about 400/mL. After 10 hours the concentration has gone up to 2000/mL. Determine the
    initial concentration of cells.

    3. Researchers found that in order to produce a recombinant protein from an E. Coli


    population they needed a cell density of 400 million cells/L. How long would it take to
    reach this cellular density if the initial inoculation density was 10,000 cells/L the glucose
    concentration was 100g/L and a small concentration of Apoptosis Inducing Factor was
    seen in the growth medium of 5g/L? Use the following information.
    kp,max=1.3hr-1
    =0.02hr-1
    Ks =2.2x10-5 g/L

    1 cell= 1ng
    4. A strain of mold was grown in a batch culture on glucose and the following data were
    obtained:
    Time (hr) Cell Concentration (g/L) Glucose Conc. (g/L)
    0 1.25 100
    9 2.45 97
    16 5.1 90.4
    23 10.5 76.9
    30 22 48.1
    34 33 20.6
    36 37.5 9.38
    40 41 0.6

    a. Find the specific growth rate ().


    b. Find the maximum cell concentration that may be obtained if 150 g/l of glucose
    were used with the same initial inoculum (at t=0)

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