Scribd Logo
Upload

Welcome to Scribd!

  • 93 views

    Benzene in Air by GC

    Uploaded by

    Gopal Mallick
    This document provides the standard operating procedure for sampling and analyzing benzene in ambient air using solvent extraction and gas chromatography. Benzene is sampled from ambient air using an activated charcoal tube and low flow sampler for 8 hours. The benzene is then desorbed from the charcoal using carbon disulfide and analyzed using gas chromatography with a flame ionization detector. The concentration of benzene in ambient air is calculated based on the amount found, extraction volume, injection volume, and air sampling rate and time.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Benzene in Air by GC

    Uploaded by

    Gopal Mallick
    93 views2 pages
    This document provides the standard operating procedure for sampling and analyzing benzene in ambient air using solvent extraction and gas chromatography. Benzene is sampled from ambient air using an activated charcoal tube and low flow sampler for 8 hours. The benzene is then desorbed from the charcoal using carbon disulfide and analyzed using gas chromatography with a flame ionization detector. The concentration of benzene in ambient air is calculated based on the amount found, extraction volume, injection volume, and air sampling rate and time.

    Original Description:

    Benzene by air by gc

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document provides the standard operating procedure for sampling and analyzing benzene in ambient air using solvent extraction and gas chromatography. Benzene is sampled from ambient air using an activated charcoal tube and low flow sampler for 8 hours. The benzene is then desorbed from the charcoal using carbon disulfide and analyzed using gas chromatography with a flame ionization detector. The concentration of benzene in ambient air is calculated based on the amount found, extraction volume, injection volume, and air sampling rate and time.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    93 views2 pages

    Benzene in Air by GC

    Uploaded by

    Gopal Mallick
    This document provides the standard operating procedure for sampling and analyzing benzene in ambient air using solvent extraction and gas chromatography. Benzene is sampled from ambient air using an activated charcoal tube and low flow sampler for 8 hours. The benzene is then desorbed from the charcoal using carbon disulfide and analyzed using gas chromatography with a flame ionization detector. The concentration of benzene in ambient air is calculated based on the amount found, extraction volume, injection volume, and air sampling rate and time.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    SOP for the sampling and analysis of Benzene in Ambient Air (Solvent

    extraction and analysis on gas chromatograph)


    1. Principle Benzene is sampled in ambient air sucked through the tube used for
    collection of BTX Sample in activated charcoal using low flow sampler. Desorption of
    the adsorbed benzene is done using carbon sulphide (CS2). The substances desorbed
    in the carbon di sulphide (CS2) are analyzed by capillary gas chromatography using
    flame ionization detector (FID) for the analysis while qualification is performed
    using the internal / external standard.
    2. Apparatus/ Instrument Required:
    a) Organic Vapor sampler APM 850.
    b) Activated charcoal tube.
    c) Gas chromatograph with flame ionization detector (FID)
    3. Reagents and Chemicals:
    a.
    b.
    c.
    d.

    Standard benzene (Sigma)


    Carbon di Sulphide (CS2)
    Fused silica capillary columns (length: 30M x. 0.25mm)
    Ultrasonicator

    4. Procedure for Sampling: Ambient Air sampling performs for benzene using
    activated charcoal/tube and low flow sampler commonly per 8 hrs. Samples are
    collected in glass tube filled with a activated charcoal tube at two ends, connecting it
    to a sample pump and pulling air through the tube with the pump. Air borne
    chemicals are trapped onto the surface of the sorbent.
    a) Two tubes are used in a series and sampling is carried out using low flow
    sampler.
    b) Keep the tube in a vertical position during sampling to prevent the possibility of
    channeling that can lead to under sampling.
    c) Sampling flow rate in the range of 20-100 ml/ min is required (2%) for Ambient
    Air.
    d) Sealed the tube with push on caps for the analysis.

    5. Analytical Procedure: Sample collected through active sampling and extracted or


    desorbed by conventional solvent (generally 1-5ml of carbon di sulphide) using
    ultrasonication for 15min to remove analyte from the sorbent material. Desorbed
    sample are analyzed using gas chromatograph filled with capillary column and
    flame ionization detector. (FID)
    GC Condition:Column- 5% phenyl 95% di- methylpalysiloxane 30M x 0.25mm
    Temperature programming
    Injection part: 250C
    Detector: 300C

    Column/oven: 50c (Hold 3 min)


    Ramp 1 @10C /Min to 140C (1min)
    Ramp 2 @20C /min to 240C (1min)
    Injection: Volume 1 l, 5l
    Benzene Concentration (g/m3) at ambient condition-

    C x V1 X 103
    V2 X V3
    C= Amount of Compound found Injection sample
    V1= Total volume of the sample extracted on ml
    V2= Volume of sample extract injection into GC
    V3= Volume of air sucked through the tube in m3, V3=(St)/106
    Where S=Sampling rate, in ml /min. & t= Sampling time, in min.

    Calculation:
    Reference (1) methods for measurement for Air pollution for sampling and analysis of
    Benzene Toluene, and xylene (BTX) as per IS: 5182 (P-11) - 2006.

    You might also like