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    Histologic Techniques PDF

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    Eya
    This document outlines the 12 basic steps of histologic techniques: 1) Numbering, 2) Fixation, 3) Dehydration, 4) Clearing, 5) Infiltration, 6) Embedding, 7) Blocking, 8) Trimming, 9) Sectioning, 10) Staining, 11) Mounting, and 12) Labeling. It provides a brief description of each step, including fixing tissues to prevent decomposition, removing water through dehydration with alcohol, clearing with xylene to remove alcohol, infiltrating with paraffin wax, sectioning very thin tissues, staining typically with hematoxylin and eosin, and mounting stained slides with coverslips. The overall goal is to properly

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    Histologic Techniques PDF

    Uploaded by

    Eya
    364 views18 pages
    This document outlines the 12 basic steps of histologic techniques: 1) Numbering, 2) Fixation, 3) Dehydration, 4) Clearing, 5) Infiltration, 6) Embedding, 7) Blocking, 8) Trimming, 9) Sectioning, 10) Staining, 11) Mounting, and 12) Labeling. It provides a brief description of each step, including fixing tissues to prevent decomposition, removing water through dehydration with alcohol, clearing with xylene to remove alcohol, infiltrating with paraffin wax, sectioning very thin tissues, staining typically with hematoxylin and eosin, and mounting stained slides with coverslips. The overall goal is to properly

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    This document outlines the 12 basic steps of histologic techniques: 1) Numbering, 2) Fixation, 3) Dehydration, 4) Clearing, 5) Infiltration, 6) Embedding, 7) Blocking, 8) Trimming, 9) Sectioning, 10) Staining, 11) Mounting, and 12) Labeling. It provides a brief description of each step, including fixing tissues to prevent decomposition, removing water through dehydration with alcohol, clearing with xylene to remove alcohol, infiltrating with paraffin wax, sectioning very thin tissues, staining typically with hematoxylin and eosin, and mounting stained slides with coverslips. The overall goal is to properly

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    364 views18 pages

    Histologic Techniques PDF

    Uploaded by

    Eya
    This document outlines the 12 basic steps of histologic techniques: 1) Numbering, 2) Fixation, 3) Dehydration, 4) Clearing, 5) Infiltration, 6) Embedding, 7) Blocking, 8) Trimming, 9) Sectioning, 10) Staining, 11) Mounting, and 12) Labeling. It provides a brief description of each step, including fixing tissues to prevent decomposition, removing water through dehydration with alcohol, clearing with xylene to remove alcohol, infiltrating with paraffin wax, sectioning very thin tissues, staining typically with hematoxylin and eosin, and mounting stained slides with coverslips. The overall goal is to properly

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 18

    Basic

    Histologic
    Techniques
    Alvin Rey Flores, RMT, MT(ASCPi),MPH

    12 Steps of Histologic
    Techniques
    12 Steps
    1. Numbering
    2. FixaGon


    3. DehydraGon
    4. Clearing
    5. InltraGon
    6. Embedding

    7. Blocking
    8. Trimming
    9. SecGoning
    10. Staining
    11. MounGng
    12. Labeling

    Numbering
    First and Most Important Step
    Logging in a Log book the details of the Gssue

    Receiving of Specimens
    Tissue Specimens should be properly labeled and with a
    corresponding request
    Tissues will be described by the pathologist (Gross
    DescripGon)
    Tissues will be cut by the pathologist

    Fixation
    Tissues are immersed in a xaGve to prevent decomposiGon,
    and to preserve the structure

    Dehydration
    Removal of Excess Water Using Ascending Grades of Alcohol

    Clearing (Dealcoholization)
    Removal of excess alcohol; makes Gssues clear or transparent;
    prepares Gssue for paran impregnaGon

    InGiltration
    Tissues caviGes are saturated with paran wax

    Embedding
    Impregnated Tissue is Oriented and Embedded in Paran
    Block to form a Tissue Block

    Blocking and Trimming


    SeparaGon of embedded Gssues into Blocks
    Removal of Excess Paran Wax using Knives

    Sectioning
    Very Thin SecGons of Tissue are Cut using a Microtome

    Staining
    Using H and E (RouGne Stain)

    Mounting
    Stained Tissue SecGons on a slide are added with mounGng
    medium and are Covered with a Coverslip.

    Labelling

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