Survey Report

on
Status of Chemical and Microbiological
Laboratories of Nepal

Standards, Metrology, Testing and Quality Forum Nepal

(SMTQ Forum Nepal)
Kathmandu, Nepal

November 20, 2010

Standards, Metrology, Testing and Quality

Forum,Nepal
Address:
c/o Lalitpur Valley College,
Jawalakhel, Lalitpur, Nepal
Telephone: 977 1 4222218
Mobile: 9841351742
E-mail: [email protected]
Website: http://www.smtqnepal.org

Acknowledgement

The SMTQ Forum Nepal, as a start of its program activities, had in the past, organised a
workshop on Quality Requirements in Honey Value Chain in Kathmandu in February
2008, and follow-up activities were pursued. The Forum this year realised the need for
encouraging food and other testing laboratories for enhancing their capacities to provide
internationally recognised conformity testing services. In this context, it decided to conduct a
quick survey of the prominent laboratories of Nepal as a stock taking exercise to access the
situation and existing service facilities in terms of physical infrastructures, laboratory
equipments/instruments and human resources available for providing test services.
The SMTQ Forum Nepal, with the technical and financial support from PTB/Germany,
assigned Mr. Pramod Koirala , as an expert to undertake the survey and submit a report . The
key findings of the survey were later presented in an interaction program held on September
21, 2010, to validate the results report. Incorporating further inputs received during the
interaction program, the report of Mr. Koirala has been finalised for publication.
The SMTQ Forum Nepal extends its gratitude to PTB/Germany and GTZ/Include
Programme in Nepal for their financial and technical backstops for conducting the survey and
also for organising a half day interaction program. The forum is also grateful to Mr. Pramod
Koirala for his valuable contribution in conducting the survey and report writing. The Forum
also expresses its appreciation to Dr. Deva Bhakta Shakya for his guidance and technical
support extended to finalise the report.
Last but not the least, the Forum also extends its deep sense of gratitude to the management
and the laboratory heads of all the 18 laboratories under survey for their cooperation in
providing information as sought. The forum is confident that the participating laboratories
under survey and other laboratories, which participated in the interaction program, will
definitely take this as useful information and will establish a very good working relationship
with the Forum.
Poorna Prasad Manandhar
Chairperson
SMTQ Forum Nepal
November, 2010

List of Acronyms
AAS: Atomic Absorption Spectrophotometer
AOAC: Association of Official Analytical Chemists
APHA: American Public Health Association
APMP:
BOD: Biological Oxygen Demand
BSI: British Standards Institute
CFL/DFTQC: Central Food Laboratory, Dept.of Food Technology and Quality Control
COD: Chemical Oxygen Demand
CAC: Codex Alementarius Commission
CEMAT: CEMAT Pvt. Ltd.
CRM: Certified Reference Material
CL/DMG: Chemical Laboratory, Dept. of Mining and Geology
DO: Dissolved Oxygen
ENPHO: Environment and Public Health Organisation
GC: Gas Chromatography
GCMS: Gas Chromatography Mass Spectroscopy
GLP: General Laboratory Practices
HPLC: High Pressure Liquid Chromatography
ISO: International Organisation for Standardisation
MRA: Mutual Recognition Agreement
NABL: National Accreditation Boards for Testing and Calibration Laboratories, India
NBSM: Nepal Bureau of Standards and Metrology
NEPLAS: Nepal Laboratory Accreditation Scheme
NESS: Nepal Environmental and Scientific Services ( P) Ltd.
NFL: National Forensic Laboratory
NGO: Non-Governmental Organisation
NARC/FRU: Nepal Agriculture Research Council, Food Research Unit
NML/DDA: National Medicine Laboratory, Dept.of Drug Administration, previously DRL
NPRL/DPR: Natural Products Research Laboratory, Dept. of Plant Resources
NPRL/NAST: Nepal Academy of Sci.& Technology, Natural Products Research Lab.
NRF: Nepal Research Foundation P.Ltd., Birgunj
NS: Nepal Standard
QA: Quality Assurance

QAP: Quality Assurance Program

QM: Quality Manual
PPP: Public-Private Partnership
PT: Proficiency Testing
RM: Reference Materials
SMTQ: Standard, Metrology, Testing and Quality Assessment
SOP: Standard Operating Practices
SPS: Sanitary and Phyto sanitary Measures
STL: Soil Test Pvt. Laboratory
TP: Technical Procedures
VRLS: Vishwa Laboratory and Research Services
VSDA: Veterinary Standards and Drug Administration, Dept. of Livestock Services
WAS: Water Analysis Services
WETC: Water Engineering and Training Centre
WHO: World Health Organization
WTO: World Trade Organization

Table of Contents

Status of Chemical and Microbiological Laboratories in Nepal

1.
2.
3.

Introduction.............................................................................................................1
Methodology of the survey:....................................................................................1
Major findings.........................................................................................................3
a. Common commodities for laboratory analysis.......................................................3
b. Commonly analyzed parameters.............................................................................4
c. Human resources and trainings...............................................................................4
d. Available Analytical Instruments/Equipments........................................................6
e. Maintenance status of equipments and agencies involved......................................7
f. Quality Manuals and Management System.............................................................8
g. Status of Laboratory Accreditation..........................................................................8
h. Availability of Reference Materials (RM) and Certified Reference Materials
(CRM)............................................................................................................................9
i. Use of Control Chart and Statistical Quality Control..............................................9
j. Method Validation....................................................................................................9
k. Reference Method Used for Analysis......................................................................9
l. Measurement of Uncertainty...................................................................................9
m. Participation in Proficiency Testing (PT) and Status of PT result.........................10
n. Practice for Internal Audit, Complaint Handling, Corrective and Preventive
Actions and Management Review...............................................................................10
o. Accommodation and Environment........................................................................11
4.
Conclusions...........................................................................................................12
5.
Recommendations.................................................................................................14
Annexes........................................................................................................................1-48

Status of Chemical and

Microbiological Laboratories in
Nepal

Abstract
In order to know the existing status of chemical and microbiological laboratories in Nepal, eighteen
different public and private laboratories were surveyed. Among the surveyed laboratories, nine were
public, seven were private, and one was NGO and one established on a public- private partnership
basis. Their status of technical manpower, laboratory test facilities, equipment status, availability of
reference and certified reference materials, practice of method validation, proficiency testing,
environment and accommodation were assessed. The result showed that all the public and private
laboratories have yet to implement/adopt international standard ISO 17025(General requirements for
the competence of testing and calibration laboratories) for establishment and operation of their
laboratories. However some of the laboratories are in a process of adopting ISO 17025. They have
joined NEPLAS scheme, introduced by Nepal Bureau of Standards and Metrology. These laboratories
are also participating in the proficiency testing and following the guidelines of NEPLAS scheme. This
study strongly recommends that all the laboratories must be encouraged to adopt ISO 17025 by
introducing promotional policy by the Government through schemes or public private partnership
programs. An apex body for laboratory accreditation should be established and strengthened for
international recognitions. However, unless such accreditation body is established in Nepal,
laboratories of Nepal should make attempts to get accreditation from any of the internationally
recognized accreditation boards in the neighboring countries. Similarly, at the implementation level,
sharing of equipments, methodologies and experience with different public and private laboratories
should be made. Adequate training and awareness programmes are also essential.

1. Introduction
Data generation by the laboratory are very crucial and indispensable tool so as to support the fact of
investigation and to take decision for necessary action in various fields such as food safety and
quality assurance programme, monitoring environmental issues, drug, assuring the status of mineral
composition, as well as bioactive components. The success of any kind of investigation and decision
is directly associated with the success of the laboratory in producing data very near to the true value.
At present, the importance of laboratory is ever increasing due to the present situation of
environmental threat, technological development, consumer censuses on health and safety, crime
investigation, international trade and regulatory requirements.
In case of Nepal, the operation of laboratory services had begun around 4 decades back in the public
sector. Gradually within past two decades, private sector also started getting involved in establishing
test laboratories and providing related services. By now there are more than 25 service laboratories in
operation. However, comprehensive information about the situation and status of the laboratories
available within the country are still lacking in a systematic and documented form. So the present
study on the Status of Chemical and Microbiological Laboratories in Nepal is conducted with the
following objectives.
1.
2.
3.
4.
5.

To develop an inventory of chemical and microbiological testing laboratories at public

and private laboratories located in Kathmandu valley and in outside valley..
To disseminate information about the testing facilities among all stakeholders or
organizations in the respective fields.
To collect information on testing facility, equipments, expertise status of coordination and
cooperation.
To publish so collected information in the SMTQ forum Nepals' website.
To assess the laboratories in the area of required training for analysis and also the
proficiency testing activities

2. Methodology of the survey:

Eighteen laboratories (9 public, 7 private, 1 NGO and 1 PPP) were selected for survey, on the basis of
the TOR (Annex 1) assigned by SMTQ Forum, Nepal to the consultant (Table 1).
Information about testing facilities, expertise, status of coordination and cooperation among
laboratories were gathered using the questionnaire given in Annex 2. The name and address of the
laboratories, along with the name of the contact person is given in Annex 3. The steps followed were:

Survey questionnaire was developed and pre-tested.

Required laboratory information were collected, as per the questionnaire, visiting laboratory
premises and head offices and concerned laboratory chief and respective personnel were also
interviewed for getting management related information.
Collected information was compiled and final report prepared.

Table 1: Laboratories, under different sectors, selected for survey

Laboratories
Government

Central Food Laboratory, Department of Food Technology and Quality Control ,

Kathmandu ( CFL/DFTQC)

Nepal Bureau of Standard and Meteorology, Kathmandu, (NBSM)

Chemical Laboratory, Department of Mines and Geology, Kathmandu(CL/DMG)

Natural Products Research Laboratory, Nepal Academy of Science and

Technology, Lalitpur (NPRL/NAST)

Food Research Unit, Nepal Agriculture Research Council, Lalitpur ( FRU/NARC)

Nepal Environmental and Scientific Services (P.) Limited, Kathmandu, ( NESS)

Environment and Public Health Organization, Kathmandu (ENPHO)

SEAM/N Laboratory, Biratnagar ( SEAM/N)

Veterinary Standards and Drug Administration, Kathmandu (VSDA)

Natural Products Research Laboratory, Department of Plant Resources,
Kathmandu (NPRL/DPR)
Drug Research Laboratory, Department of Drug Administration,
Kathmandu(DRL/DDA) , Now, National Medicine Laboratory ( NML)

National Forensic Laboratory, Lalitpur ( NFL)

Private sector
Soil Test (P) Limited, Kathmandu (SLT)
Cemat Pvt. Limited, Kathmandu ( CEMAT)
Water Engineering and Training Center, Kathmandu (WETC)
Vishwa Laboratory and Research Service, Kathmandu( VRLS)
Water Analysis Services , Kathmandu (WAS)
Nepal Research Foundation P Ltd, Birgunj (NRFL)

NGO
PPP

3. Major findings
a. Common commodities for laboratory analysis
Most common types of products testing services provided by laboratories under survey are as given in
Table 2.
Table 2: Types of Products Testing Services Provided by Laboratories under Survey

Government laboratories perform the regulatory services, therefore, the commodities normally
received are the samples collected under the regulatory objectives. Water is the major test material for
private laboratories. The major food products analyzed by the Government laboratories are water,
noodles, edible vegetable oils, processed confectionery items (biscuits, chocolates) instant noodles,
alcoholic beverages. The common commodity for NPRL/DPR is herbs and essential oil. The other
public laboratory viz., NML, is getting drug samples of capsule, liquid, ointment, injection for
analysis. Rocks, ore, minerals, coal and other geochemical samples were the common commodities
for CL/DMG.

The major products analyzed in private laboratories are water, waste water, soil, construction
materials (such as sand, cement, limestone). Some private laboratories have their expertise in analysis
of environmental parameters such as laboratory analysis of air quality and waste water. NRF in
Birgunj has their expertise in analysis of pharmaceutical products along with food and environment
monitoring parameters and analysis of packaging materials. Information on analytical capability of
different surveyed laboratories for common commodities and is given in Annex 4.

b.

Commonly analyzed parameters

All private laboratories are found customer-oriented and therefore, most of these laboratories are well
equipped with the advanced test facilities. The common parameters used for analysis in the private
laboratories include physical, chemical and microbiological parameters. Certain minerals in water,
such as iron and arsenic are the most common chemical test parameters and pH, hardness are very
common among physical test parameters.
In the Government laboratories (DFTQC, NBSM), general focus is on the analysis of safety
parameters in processed food products. In the NPRL/DPR, the parameters analysed are related to the
identification of herbs and purity analysis of essential oil. In NML, identification and quantitative
analysis of allopathic drugs are made. The physical tests for disintegration, dissolution are the major
physical parameters under consideration for capsules. Currently, vaccine analysis facility was not
available in NML.
The mineral matters, moisture and acid insoluble ash are the major test parameters in rocks and ore at
CL/DMG. In the case of coal, fixed carbon, sulfur, calorific value was analyzed.
The test parameters under analysis in the laboratories of DFTQC and NBSM are those common
parameters mentioned in the mandatory food standard and voluntary NS standards, respectively. For
example, in fruit beverages, preservatives were analyzed. Among other safety parameters,
microbiological analysis is also quite common parameters for most of the food products. The
proximate analysis is also common to several food products. Minerals such as Calcium and
Magnesium are also analyzed frequently in the case of construction materials such as soil, cement,
rods and sands. Information on common parameters is summarized in Annex 3.

c.

Human resources and trainings

Analysts and supervisors should have relevant education, training and experience to perform the
assigned tasks, which are key requirement for a successful laboratory.
The survey revealed that there are around 112 technical personnel who are directly involved in the
laboratory services in 18 laboratories, with almost half of them are chemists. (Table 3) . Almost 64
percent of the technical personnel are concentrated in Govt. laboratories.

Table 3: Human Resource in the Laboratories of Nepal Surveyed

Public laboratory analysts regularly receive training/orientation courses on different aspects of

laboratory management, both in-country and abroad. Presently, private laboratory personnel are also
getting exposure gradually to ISO 17025. During the study it is found that more experienced persons
are found working in private laboratories than in public laboratories. In most of the private
laboratories retired government analysts found working, which possess good skills and experiences.
They were found trained in analytical work; however, they were poorly trained in ISO 17025. The
number of analysts in private sector ranges 3 to 4 in private laboratory; however, in public laboratory
the number is higher-depending on Government system. Among the surveyed private laboratories,
NRF was with the highest number of staff (twelve staff) working in the laboratory and with the
experience of 3 to 10 years. Availability of human resource and training in different laboratories is
given in Annex 4.
There are well qualified personnel currently working in the private as well as public laboratories.
They have academic qualification in their respective field. However, they have never gone to the
refresher course in their respective laboratory analysis work. In different laboratory analysis areas
refresher course is felt much necessary to upgrade their current skill. This will help laboratories to
acquire ISO 17025:2005 accreditation in future. Table 4, given below, shows the number of technical
personnel available in Government and private laboratories, who are well trained to operate some of
the new generation instruments.

Table 4: Number of Operators handling Testing Equipments /Instruments in different

Laboratories

d.

Available Analytical Instruments/Equipments

Equipments should be adequately inspected, cleaned, and maintained and should be properly tested,
calibrated and/or standardized. Written SOP requires with detail test methods, schedules for routine
inspection cleaning, maintenance, testing, calibration and or standardization of equipment. Methods
and procedures of checks and maintenance on a routine basis for calibrations required. Method of
checking and maintenance after use of instrument (washing, drying, sterilization, disposal etc) is also
necessary. Plan of action for maintenance of equipments on a periodical basis is also necessary.
UV and Visible spectrophotometer is the most common equipment in all laboratories. These
spectrophotometers are used for the analysis of minerals in water and in food products. AAS is the
other commonly used equipment in both public and private laboratories. The major use of this
equipment is for the analysis of minerals and heavy metals in water.
Some laboratories have their expertise in specific parameters related to environmental analysis. They
are analyzing air quality and waste water. In order to analyze waste water, BOD and COD
measurement equipments, such as BOD incubators and DO meters are available in the some of the
laboratories like ENPHO, STL and WETC.
Some private laboratories (WETC and NRF) acquire modern analytical equipment such as GCMS
(with ECD and FID detector) and LCMS. This is a good initiative taken by the private sector
laboratories. The details of equipments owned by different laboratories are given in annex- 5.
However, a summary list indicating numbers of major laboratory instruments/equipments available in
public and private laboratories are shown in Table 5. The status of utilization of some of the major
laboratory instruments / equipments in the private sector laboratories are reflected in Table 6 ( also in
Annex 11)
Table 5: Availability and Use of Some important Instruments

Table 6: Utilization of some of the major laboratory instruments / equipments in the private
sector laboratory

In many laboratories, calibration of equipments has not been made so far. In some cases in private
laboratories, in-house calibrations of equipments are reported to have been made.

e.

Maintenance status of equipments and agencies involved

The maintenance of equipment is guaranteed by the supplier, usually for the period of one year, at the
time of purchase of equipments. The suppliers deal with maintenance of machine and equipments and
usually for one year which is the warranty period for most of the equipments. Bandana Trade
Concern, Kathmandu is one of the agencies helping laboratories for maintenance of equipments.
Expect NRF in Birgunj, the other laboratories have not recruited any maintenance agencies on a
contractual basis. NRF has the annual maintenance contract with Tosvin Analytical, New Delhi, India.
In many laboratories, some instruments and equipments are either lying idle or not in operational
stage. Numbers of some key instruments/equipments which are non-operational are given in Table 7.
Table 7: Numbers of Important testing instruments/equipments, which are non-operational

The laboratories reported that they werent satisfied with the maintenance service providers. The
number of persons trained in this area was also less in this field of equipment maintenance. The SOP
and OMs for equipments were available in all laboratories .The numbers of staff who can run the
equipment vary from two to four in most of the laboratories, both at the public and private sector.

Maintenance and calibration status of instruments/ equipments in all laboratories are given in some
details in Annex 6 and summarized in Table 8.
Table 8: Status of Maintenance and Calibration Practices in different Laboratories

f.

Quality Manuals and Management System

Quality manual is a document stating and describing the quality system of a laboratory. Quality
Manual (QM) (Policy on quality, quality management structure, procedures) is a working document
made available to all staffs for day-to-day use. It is a comprehensive document and provided with
subsidiary documents for laboratories and should have Technical Procedures (TP) along with Quality
manuals. Private laboratories claim that quality management system exists according to NEPLAS
system based on ISO 17025:2005. Amongst public laboratories, quality manual is only available in
DFTQC and NBSM. Status of quality manuals and management system of different laboratories is
given in Annex 6.

g.

Status of Laboratory Accreditation

Laboratory accreditation is a general requirement for the competence of testing and

calibration laboratories. It is the international standard used by laboratories in developing
their quality system and technical system that govern their operations. ISO 17025:2005 is the
standard specification of general requirements to be met by testing and calibration
laboratories according to their organization and management of quality. The benefits of
laboratory accreditation are that:
It provides formal recognition to competent laboratories and ensures that they perform their work
in accordance with international criteria.
It minimizes the risk of unreliable results which, in turn, reduces the risk for manufacturers or
suppliers to produce or supply a faulty product.
It minimizes the chances of retesting and hence reduces chances of additional financial burden
and time delays
It enhances customer confidence and satisfaction.
The test results are internationally accepted provided the laboratory is accredited by any
internationally recognized accreditation body. Based on mutual evaluation and acceptance of
other countrys laboratory accreditation systems, international agreements called Mutual
Recognition Arrangements (MRA) have been established for realizing the ideal of having
products tested once and accepted everywhere. Such agreements are crucial in enabling test
data by an accredited laboratory to be accepted in overseas markets and facilitate trade.
So far, none of the laboratories of Nepal has been accredited by any internationally recognized
accreditation body.

All the surveyed private sector laboratories and one public and private partnership lab (SEAM/N)
have been found to be accredited only with NEPLAS scheme of NBSM. Therefore, all the
laboratories will have to adopt international standard ISO: 17025 while providing testing services, so
as to be able to get international recognition through Laboratory accreditation. Public sector
laboratory doesn't have accreditation even with NEPLAS scheme. DFTQC is in the process of
accreditation with NABL, India for some test parameters. Two projects under UNIDO/EC-NepalWTO and PTB/Germany are supporting DFTQC and NBSM for acquiring Laboratory accreditations.
Name of the accredited laboratories under the NEPLAS scheme is given in Annex 7.

h.

Availability of Reference Materials (RM) and Certified Reference Materials

(CRM)

Generally, CRMs are available to both private and public laboratories. Minerals such as (iron, zinc,
copper, mercury, arsenic and ammonia and phosphates) and some other mixed heavy metals are the
most common CRMs available in these laboratories. Similarly, DFTQC is also having CRM of
pesticides, myco-toxins and microbiological tests. WHO and laboratories of Denmark and Singapore
are also supporting NML/DDA for CRMs for drug testing. In case of NPRL/DPR, CRMs for essential
oil are available. Availability of reference materials and certified reference materials is given in
Annex 6.

i.

Use of Control Chart and Statistical Quality Control

Amongst public laboratories, mean control chart is being used only in NBSM. Only two private
laboratories (CEMAT and WETC) are found using confidence limit chart. Status of use of control
chart and statistical quality control is given in Annex 6.

j.

Method Validation

The current standard methods (like APHA, BSI, AOAC, ISO, Pharmacopeia, CAC), should be
applied and validate if published or in-house against standard method. Quality control samples should
also be tested to validate test conditions (reference, certified materials, standards etc). Practice of
method validation among the surveyed laboratories is given in Annex 7. Excepting ENPHO
Laboratory, no other laboratory seems to have initiated method validation. ENPHO has claimed such
initiatives for selective parameters like BOD & COD.

k.

Reference Method Used for Analysis

Internationally approved analytical test method (such as AOAC, ISO, IS, APHA) are used in both
public and private laboratories. Public laboratories have developed their own manuals for analysis
using internationally accepted test methods such as APHA and AOAC. Private laboratories follow
NBSM test method supplied under NEPLAS certification scheme. At CL/DMG, Vogel reference
method is commonly used for chemical analysis. Reference methods used for analysis in the different
laboratories are given in Annex 6.

l.

Measurement of Uncertainty

None of the laboratories are found involved in uncertainty measurement. However, some laboratories
reported that they are making attempts to start practicing the measurement. In WETC, it is observed
that uncertainty measurement for pH was complete and similarly for metal conductivity was found in

progress. Similarly, ENPHO is also found involved in progressing measurement of uncertainty in

nitrogen percentage determination. Status of measurement of uncertainty is given in Annex 6.

m.

Participation in Proficiency Testing (PT) and Status of PT result

All the private laboratories accredited under NEPLAS scheme are participating in the proficiency
testing with NBSM. Those laboratories are getting proficiency test results regularly. Public
laboratories like DFTQC, NBSM and NFL are participating in international PT programs. DFTQC is
participating in PT program run by PTB and results are satisfactory. NBSM is reported to have been
participating in some PT programs of Asia Pacific Metrology Program (APMP) for cadmium in rice
products, cadmium and lead in herbal products, pesticides in Tea. NFL is also participating in PT
programs with GTAD, Spain. NML has not participated in any PT program yet, but has planned to
join with PTB for proficiency testing. NPRL/DPR has participated in PT for pH and TDS whereas
CL/DMG has participated PT in potassium in water. VLRS has also participated in PTs conducted by
NBSM. Laboratories of DFTQC and NBSM are participating in PT program with PTB. Participation
in PT by different laboratories and their status is given in Annex 8.
Table 9: Laboratories with their Quality Management Systems

n.

Practice for Internal Audit, Complaint Handling, Corrective and Preventive

Actions and Management Review

There should be a provision for planning, conduction and reporting of internal audit. Corrective
actions and management review meeting is also necessary. More than half of the private laboratories
under survey have reported that they have regular internal auditing (Table 10). Audit report is also
mandatory requirement for continuation of NEPLAS accreditation. In public laboratories, internal
audit and management review aren't taken as priority programs. Only one out of nine government
laboratories is practicing the internal auditing whereas six have management review and laboratory
safety measures introduced. Only one third of the laboratories have followed the practices of
complaint handling, corrective and preventive actions. The status of practice of internal audit,
complaint handling, corrective and preventive actions and management review for different
laboratories is given in Annex 9.

Table 10: Number of Laboratories Adopting Additional Laboratory Practices

o.

Accommodation and Environment

Facilities and resources conducive to perform valid testing procedures and workflow should be
sufficient to prevent cross-contamination. Facilities should comply with relevant guidelines and
regulations relating to health and safety. It requires good housekeeping and monitoring of
environment conditions. The working environment and cleanliness of all the laboratories are yet to be
in good conditions. Laboratory disposal system wasnt managed properly in both public and private
laboratories. The control of temperature and dust and humidity is limited to some specified areas
according to need of the equipments and is found not the priorities of the laboratories, both public and
private (Table 11). Only a third of the laboratories do have temperature controlled rooms. Control of
humidity and dust is virtually non-existing. Some detailed status of laboratory accommodation and
environment is given in Annex 10.
Table 11: Environment Control Facilities of the Laboratories

4. Conclusions
Laboratories need to produce quality data in a cost effective manner. The serious deficiencies occur
when attention to quality is neglected or ignored. Growing poor laboratory practices have led to the
proliferation of Laboratory Accreditation, Good Laboratory Practice and Quality Management
System.
Quality Assurance Program varies from one establishment to another. It depends on nature and
purpose for which the organization exists. For successful implementation of QAP commitment,
support, understanding and cooperation of top management and staff are crucial.
Since there exist many different schemes like GLP, ISO 17205, ISO 9000 etc., the top management of
laboratories seems to be in confusion as to which scheme to be adopted. It is felt that there is a
growing pressure to harmonize them or at least establish effective Mutual Recognition Agreements
(MRA). Similarly, some laboratories have difficulties with prescriptive interpretation of ISO 17205
and ISO 9000, justified by the auditors in the interests of customer confidence. Auditors are sometime
seen as inconsistent or over concerned with secondary issues. On occasions, there are lacking in the
technical depth. Most of the laboratories are poorly informed about these standards and process of
third party registration. Some laboratories are found discouraged to any new scheme on the ground of
high investments and running costs required to be borne, consequently.
Laboratories supporting the government regulations must pay greater attention to generate quality
data, particularly that of DFTQC, NBSM and NML. The cost of achieving and maintaining regulatory
compliance (SPS, medical products) may rise as legislations are introduced.
All public laboratories have well trained manpower and usually follow international test methods or
standards, equipped with fairly advanced instruments/equipments. In public laboratories, there is a
satisfactory environmental facility. But for precision testing or calibration activities, the facilities are
yet to be satisfactory. All laboratories appeared to have insufficient documented quality system.
Most of the laboratories either do not have QC manuals or not following GLP. They do have wellqualified manpower but are not motivated enough therefore, they are taking longer time cycle while
providing testing/analytical services. There is a lack of optimal utilization of very important
instruments/equipments like GC, AAS etc. Lack of proper maintenance and calibration of instruments
and even glasswares are clearly visible. Similarly, performance evaluation is hardly being made. The
participation in international proficiency is lacking and internal quality control activities are ignored.
Furthermore, management review is not carried out and hardly internal audits are made. Practice of
referring to the measurement of uncertainties is yet to begin.
Therefore, though public laboratories have infrastructural set up and adequate manpower but due to
lack of proper management and unable to implement GLP and ISO 17025, their performance in
service delivery are yet to be satisfactory. The survey has indicated that private laboratory service
delivery is better to the customer and comparatively faster than public laboratories.
There are seven private laboratories in Kathmandu, which are accredited under NEPLAS scheme
(given in annex-7). These private laboratories are participating in the proficiency testing coordinated
by NEPLAS. There is a limited scope of operations and documented QA programs (manual, SOPs).
Laboratory personnel are motivated in quality works in those laboratories where internal audits and
management reviews are regularly conducted. It is also found in private sector laboratories that
suitable preventive approaches have been taken for fixing the breakdown and for the maintenance of
equipments/instruments.

Based on the observations made from the survey, and international practices and requirements, the
opportunities and constraints of existing laboratories in the country are as follows:
Opportunities
Experience, trained , sufficient manpower in some laboratories
Already accreditation scheme-NEPLAS scheme
Participation in proficiency testing by private laboratories
Laboratories are equipped with advance equipments
Prospects of expanded service providing role
Prospect of Government and Donors technical supports in capacity building
Constraints
Infrastructural limitation
Lack of management commitment-more in public laboratories
Resource constraint
Insufficient mutual cooperation and team work
Lack of sharing of knowledge
Lack of proper training
Insufficient maintenance of instruments/ equipments
Insufficient motivation
High health risk factors in work and inadequate safety measure
Poor carrier advancement opportunities
Poor recognition of work
Inconsistency in work
Some gaps have also been identified at the policy and implementation level.
a.

.
b.

Policy gaps
Policy and priority for Laboratory accreditation is lacking both in Govt. and private sector.
The public-private partnership among the laboratories is virtually non-existing
Internationally recognizable Laboratory Accreditation Board is yet to be formed.
Existing reference laboratories for proficiency testing and calibration of equipments have
facilities very limited and could cover only few measurements like mass, temperature and
force etc and some analytical test parameters .
Inter laboratory proficiency testing facility is also not available adequately to address the
present needs.
Government laboratory hasn't accredited yet. Accreditation of Government laboratory will
create demand pull for private laboratories.
Gaps at implementation level
The working environment and cleanliness of the laboratory isn't encouraging.
Quality manuals arent updated timely.
Most of the available technical personnel are not trained to handle and use the specialized
instruments and are not motivated.
Reference materials aren't available in many laboratories.
Waste disposal system isnt managed properly.
Instruments/Equipments are not maintained. Many are either not in operation or out of order.
Lab glassware are not of the standard brands

Awareness program/refresher courses offered are insignificant to cater for up-gradation of

knowledge Similarly , there is no opportunities for laboratory personnel for exposure visits,
observation tour
Calibration of equipments and glasswares (In-house /Accreditation) is very rare.
Intra laboratories coordination is very poor and there is no proper documentation system in
the laboratory.

5. Recommendations
At policy level
Government should encourage laboratory accreditation at policy or organization for lab
control, establishment, monitoring and management and dissemination of authority.
Government apex body for laboratory accreditation should be strengthened
- promotion of laboratory accreditation
- invitation of key stakeholders to a round table discussion on national lab capacity building /
national quality policy
- initiation of cooperation with international technical cooperation agencies (like PTB) for
capacity building
Currently, SMTQ related projects have been supporting different public laboratories in order
to develop their laboratory capabilities. Such supports have been for the development of
human resources; technical advises and for acquiring laboratory instruments/equipments so
as to be able to acquire internationally recognized laboratory accreditation for some
important test parameters. As an encouragement to the private sector laboratories for their
readiness for accreditation, strong lobby is required for the Government to agree for
outsourcing of services to competent ones. The donor agencies should also consider
extending their technical supports to the
private sector laboratories for their capacity
building, particularly to those which shows willingness to upgrade their laboratory for
meeting the requirements of laboratory accreditation
At service provider level
Central referral laboratories are essential which can work as center for excellence for all the
laboratories in the country, which will act as the national PT providers. The capacities of the
laboratories identified for the purpose should be further strengthened to be able to perform its
tasks in accordance with international good practices. These laboratories should help other
service laboratories for carrying out calibration and proficiency testing, method validation,
management of reference materials and laboratory safety. There is a need for calibration of
equipments and glassware. This laboratory could also be utilized for continual improvement
of methodology and manpower in the different laboratories.
There should be one common forum of public and private laboratories. SMTQ Forum Nepal
could take a lead to facilitate sharing of documents (eg, journals, test methods/procedures,
PT information, test results, standards), experience/ problems sharing amongst public and
private laboratories and laboratory professionals. This forum could also take leadership in the

promotion of GLP, laboratory accreditation, PT participation and other relevant trainings

required in the changed context of WTO.
Short term refresher trainings are essential to upgrade skills of laboratory personnel. The
laboratory personnel and the top managements of all the laboratories should be given
orientation on the GLP and provisions of ISO 17025 and also on how to participate in the
PTs. SMTQ Forum Nepal should take a lead to organize orientation/training programs.
Arrangements for periodic maintenance
and repair services for the key
instruments/equipments are also considered highly essential in collective basis , as getting
such services individually is very difficult, time consuming and highly costly. Prospect of
coordination of such arrangements by SMTQ forum or any other agency will have to be
explored.
At laboratory level,
There are many some recommendations that the laboratories themselves could implement,
these include:
- Improving good housekeeping, hygienic /environmental practices,
- Working on quality management documentation like control chart, quality manual, standard
operating procedures, record keeping etc.

Annexes
Annex- 1: TOR for Consultant to conduct a survey of Testing Laboratories
(SMTQ Forum Nepal)

Survey of testing laboratories as per the designated format (will be provided by SMTQ Forum
Nepal) of laboratory located in Kathmandu or in any case Birgunj and Bhairahawa.

Review the existing status and facilities of the testing laboratory and recommendations update
their capacity.

Identification of training needs particularly to the laboratory personnel and need for Proficiency
Testing (PT)

Assessment of testing laboratory for its capability for analyzing the labs for confirming the ISO
17025 requirements.

Others areas as the expert feel essential or appropriate in his/her practical experience in the field
of testing laboratories.

Annex- 2: Survey on Status of Chemical and Microbiological Laboratories of Kathmandu

Valley,Surveyor: SMTQ Forum Nepal, email: [email protected],Contact Person:
Mr. Pramod Koirala ([email protected]),Mobile : 9841730789
Section 1: General information
(to be published at STMQ-Website (http://www.smtqnepal.org)
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
a)
b)
11.
12.

Name of Laboratory:
Address:
Phone:
Email:
Website (if applicable):
Contact Person:
Mother organization (if any):
Type of Organization: GovernmentPrivate.others.
Collaboration with other laboratories and other services (if any):
Test service offers:
Common Commodities
Common Parameters
Major equipments:
Other services offered (e.g. trainings, consultancy, etc.):

Section 2: Information on capacities and training needs

(will be treated confidentially by the SMTQ-Forum)
13. Human resources
Name of Key
Designation Qualification
Experience
Major
Analysts/Managers
Training

Specialization if
any

Comments on human resources/training need if any:

12. Details on analytical Instruments
Name of
Numbe Make/Model
instruments
r of
Equip
ment
Gas Chromatography
High Performance
Liquid
Chromatography
.GCMS
LCMS
UV/Visible
Spectrophotometer

Maintenanc
e Status

Calibration
Status

Status of
instrume
nts

No of
staffs who
can run
the
equipmen
t

Availabili
ty of
operating
or other
manuals

AAS
IR
Others if any
Is there any maintenance agency defined for your laboratory equipment?
13. Quality Management System
a) Quality Management System in your laboratory is based upon ISO 17025:2005 or others
b) If your laboratory is accredited, from where it is accredited.
c) Availability of CRM
1.

2.

3.

4.

5.

d) Availability of RM
1.
2.
3.
4.
5
e) Type of Control Charts used if any
f) Method Validation carried out if any (Please mentioned even if you are in process)
g) Reference Method used for analysis
h) Uncertainty measurement carried out if any (Please mention the status even if you are in process)
i) Participation in Proficiency Testing
j) Status of the PT results
k) Is there procedure and practice for
i)
internal audit
ii)
complaint handling
iii)
corrective action
iv)
preventive action
v)
management review
vi)
Laboratory Safety system
14. Accommodation and Environment
a) Control of temperature
b) Control of dust
c) Control of Humidity:
d) Others
15. Problems or comments if any:
To the best of my knowledge the information given above is correct and valid.
Signature
Name:
Designation:

Annex 3: List of Surveyed laboratories

SN
1.
1

Name and address

Central Food
Laboratory, Department
of Food Technology and
Quality Control ,
Babar Mahal,
Kathmandu

Tel.No; email address and

website
Tel: (01) 4262369
Email:
[email protected]
website:
www.dftqc.gov.np

Contact
person
Mr Ramesh Sthapit

Commodities tested
Food and Feed

Parameters Under Tested

Biscuit, wheat flour, bakery products and confectionaries:
Moisture, free fatty acid, Total ash, acid insoluble ash, acidity of
alcoholic extract, sulphated ash, total sugar, reducing sugar, total solids,
crude fibre, gluten
Sugar and honey: Moisture, water insoluble matter , total sucrose,
total reducing sugar, total ash, sulphated ash, dextrose, fructose
dextrose ratio, starch
Noodles : Moisture, acid insoluble ash, total ash, protein, gruel content,
acidity of extracted fat, peroxide value of extracted fat, acidity of oil,
peroxide value of oil
Cheese: Moisture, fat, sodium chloride
Tea and coffee: Caffeine content, water extract, crude fibre, moisture,
total ash, water soluble ash, alkalinity of soluble ash, acid insoluble ash
Fruit beverages: Total Plate count and coliform count, total soluble
solids, acidity, benzoic acid, saccharine, drained weight, brix reading,
pH, carbon dioxide content (by pressure measurement), alcohol
content, heavy metals
Oil and ghee: moisture, free fatty, acid (acid value), specific gravity,
argemone test, peroxide value , iodine, value, saponification number,
unsaponifiable matter, refractive index, melting point, colour,
hexabromide test (linseed oil test), sesame oil test, allyl isothiocynate
test (volatile oils), rapeseed oil test, beller turbidity temperature
test(btt), mineral oil
Milk and milk products: determination of fat, SNF, Total plate count,
Coliform
Ketchup and sauce: Total solids, specific gravity, acidity as acetic
acid, benzoic acid, Yeast and mold count
Spices: Moisture, acid insoluble ash, steam volatile oil, non volatile
ether extract, alcohol extract, curcumin, lead chromate test
Feed and feed products: crude protein, free fatty acid(extracted), acid
insoluble ash and total ash, moisture, crude fat, crude fibre, calcium,
phosphorus, sodium chloride
Table salt: iodine content, sodium chloride, moisture, water insoluble,
Rice and grains: moisture, Grading parameters ( broken grains,
foreign matter, damaged grain, decolorized grain, chalky grains, red
grains)
Water: colour turbidity, total dissolved solids, total suspended solids,
pH, total chloride, total sulphate, total sulphide, acidity/alkalinity, total

phosphorus, water soluble phosphorus, total nitrogen, ammoniacal

nitrogen, total hardness, calcium hardness, turbidity
Nutrients analysis in food products : Carbohydrate, protein, fat,
vitamin C, Minerals (Ca, Fe, Mg)
Cereals and grains: Mycotoxin including aflatoxin, Pesticide: DDT,
BHC, Malathion, Parathion, Methyl Parathione
Microbiological analysis in Food and Feed: Total plate count,
Coliform count, Yeast and mold count, E. Coli, Salmonella, Shigella,
Vibrio parahemolyticus, Bacillus cereus.
2.
2

Nepal Bureau of
Standard and
Meteorology,
Balaju, Kathmandu,

Tel: (01) 4350818

Email:
[email protected]
website:
www.nepalstandards.gov.np

Mr Ramesh Khadka

Food, Feed, Water and

Effluent, Spirit, Chemicals
including detergents, cement,
paints, leather, coal,
fertilizers, textile, plastic,
petroleum products, wood,
electrical wire and cable,
pipe, steel, bag, shoes

Microbiological test : Total count, coliform count, faecal coliform,

salmonella (confirmatory), thermoduric coliform, E.coli, Salmonella,
shigella
Biscuit, wheat flour, bakery products and confectionaries: pH, net
weight, moisture, free fatty acid, Total ash, acid insoluble ash, acidity
of alcoholic extract, sulphated ash, total sugar, reducing sugar, total
solids, crude fibre, gluten
Sugar molasses and honey: Moisture, brix value water insoluble
matter, total sucrose, total reducing sugar content, total ash,
sulphated ash, calcium oxide, specific gravity, free acid and pH,
dextrose, fructose dextrose ratio, total nitrogen, starch
Noodles : Moisture, acid insoluble ash including total ash, protein,
gruel content, acidity of extracted fat, peroxide value of extracted fat,
acidity of oil, peroxide value of oil
Cheese: Moisture, fat, sodium chloride
Tea and coffee: Caffeine content, water extract, crude fibre, moisture,
total water soluble ash and alkalinity of soluble ash, acid insoluble ash
including total ash
Drinks (beverages): Total plate count and coliform count, total
soluble solids, acidity, benzoic acid, saccharine, drained weight, brix
reading, pH, carbon dioxide content (by pressure measurement),
alcohol content
Oil and ghee: moisture, free fatty, acid (acid value), specific gravity,
argemone test, peroxide value , iodine, value, saponification no,
unsaponifiable matter, refractive index (or BR reading), melting point,
colour, hexabromide test (linseed oil test), sesame oil test, allyl
isothiocynate test (volatile oils), rapeseed oil test, bellyer turbidity
temperature test (btt), mineral oil
Milk and milk products:
Determination of fat, SNF, Total plate
count, Coliform count
Ketchup and sauce: Total solids, specific gravity, acidity as acetic
acid, yeast and mold count
Spices: moisture, acid insoluble ash, steam volatile oil, non volatile

ether extract, alcohol extract, curcumin, lead chromate test

Feed and feed products: Crude protein, free fatty acid (extracted),
acid insoluble ash including total ash, moisture, crude fat, crude fibre,
calcium, phosphorus, sodium chloride, sodium bicarbonate, net weight,
energy value
Table salt: Iodine content, sodium chloride, moisture, water
insolubles, magnesium, potassium chloride
Rock salt (Bire nun): Ferrocyanide test(qualitative and quantitative)
Rice and grains: moisture, grading parameters (broken grains, foreign
matter, damaged grain, decolorized grain, chalky grains, red grains,
paddy) bulk density
Gypsum: Purity, zinc oxide
Sodium bicarbonate, caustic soda : Purity
Detergent and soaps: Active ingredient, phosphate content (as tripoly
phosphate), water insoluble matter, alcohol insoluble matter, pH,
volatile matter, moisture, total fatty matter, matter insoluble in alcohol,
unsaponofiable matter, free caustic alkali, chloride
Leather: Moisture, water soluble, oils and fats, hide substance, total
ash, total chromium, hexavalent chromium
Waters and effluents: Total dissolved solids, total suspended solids,
pH, total chloride, total sulphate, total sulphide, oil and grease, COD,
BOD, total chromium, acidity/alkalinity, total phosphorus, water
soluble phosphorus, total nitrogn, ammoniacal nitrogen, total hardness,
calcium hardness, methyl orange alkalinity, colour, turbidity
Paint: Gloss (qualitative), drying time, surface dry, hard dry,
consistency, viscosity, color, finish, scratch hardness, net
weight/volume, flexibility, stripping test, flash point, density (wt/10
litre), net volume/weight
Cement paint: Chloride, sulphate, water solubles
Cement and mortars (cement total analysis): Sand cement ratio,
calcium oxide, magnesium oxide, sulphur trioxide, iron oxide, R2O3,
loss on ignition, silica, insoluble residue
Coal: moisture, total ash, volatile matter, calorific value, caustic soda,
zinc sulphate, monohydride (ZnSO4.H2O)
Fertilizer: moisture, particle size, total nitrogen, ammoniacal nitrogen,
total phosphorus, water soluble phosphorus, biureate, potassium,
calcium,
g.i.wire, pipe and sheets: Uniformity, mass of zinc coating,
dimensional measurement, uniformity of plate, single spot , triple spot,
zinc coating, adhesive test
Textile material: Extraction of dye and sizing material, single
digestion, cotton , viscous percentage, wool percent, polyester, protein
fibre, nylon fibre, plastic lamination (percentage) , aluminium acetate

coating, polypropylene, jute, colour fastness to rubbing

Plastic container: Total migration
Petrolium product: Flash point, distillation, initial boiling point,
final boiling point, recovery at 70oC, recovery at 125 oC , recovery at
180 oC, recovery at 200 oC, total recovery, residue, moisture, viscosity,
total sediments, density, inorganic acidity, lead content
Bleaching powder/sodium hypochloride: Available chlorine, sodium
bicarbonate
Spirit: specific gravity (by distillation method), alcohol content
(distillation method), alcohol content(hydrometer method), methanol
content, miscibility with water, alkalinity, residue on evaporation,
aldehyde content, fusel oil, furfural content, ester content, total acidity,
volatile acidity, fixed acidity, permanganate reduction time
Steel: Chromium, carbon, manganese , phosphorus, sulphur, nickel,
silicon
Tooth paste: Net weight, residue on 150 micron sieve, residue on 75
micron sieve, pH, abrasion, foaming power
Purity tests of Sodium hydroxide, caustic soda, copper Magnesite:
Bulk density
Acetic acid: Freezing point, formic acid and acetic acid content
Metals identification (except aluminium)
Wood: Moisture (oven dry method, dean and stark method
Coal: Moisture, total ash, volatile matter, calorific value
Helmet: Shock absorption test, penetration resistance test, chain stap
test, electric resistance test, flammability resistance test, melted metal,
projection test, heat resistance test
Multimeter calibration:
ACSR conductors: Dimensional measurement, mass of ACSR,
electrical resistance test, tensile test, each aluminium conductor, each
steel conductor
Drop wire: Clamp (per piece)
PVC cable: Dimensional measurement, mass of PVC, conductor per
Km, electrical resistance test, insulation resistance , fire resistance test,
tensile test (PVC, conductors), test of dumbell, tensile test of copper
conductor, insulation thickness, high voltage, solder bath test
Supporting cable or self supporting drop wire (telephone ):
Diameter of conductor, resistance of conductor, insulation resistance,
insulation thickness, tensile strength of steel (any no. of wire,
elongation of copper, mutual capacitance, diameter of suspension wire,
sheath thickness (suspension), sheath thickness (wire), supporting cable
for 2 to 5 pairs, 1 ", 10 " supporting cable for 11 to 25 pairs, 26 to
50 ", 51 to above, house wire, jumper wire
Test for battery heavy duty: ro3, r6 , r14, r20

3.
3

4.

Veterinary Standards and

Drug Administration,
Tripureswor, Kathmandu

Tel: (01) 4268243

Email:
[email protected]

Mr Purnima
Manadhar

Veterinary medicine, Vaccine

, Feed, Meat and Meat
products, eggs, milk and
milk products

Natural Products
Research Laboratory,

Tel: (01)4268247

Mr Umesh K Shrestha

Medicinal plants, Aromatic

plants

Multi purpose: r6, r14, r20,

Pipes: polyethylene pipes, reversion test, hydraulic test, dimensional
measurement, density (bulk), melt flow index, pipe jointed hammer test
(welding), with test
Poly vinyl chloride : reversion test, stress relief test, dimensional
measurement test, impact test, opacity, hydrostatic test, construction
material testing labor
Physical testing of cement: Fineness, soundness, le chat, autoclave,
setting time, specific gravity
Compressive strength, cubes (prepared by the client), concrete, mortar
Bricks: compressive strength, dimension, efflorence, water absorption,
hollow cement concrete block(per 2 pieces)
Calibration: Compressive strength testing machine
Wool: LAC(length after carding) (LBM), saple length (arithmetic
mean), diameter, modulation
Woolen fabrics: percentage of wool
Yarn: Tensile strength, wt. per 100 m, breaking load, elongation
Fabric: Tensile strength, warp and weft, linear density
Paper: Grammage (gm/m2 ), thickness (micron), tensile strength,
feather (swelling test), cob value, brightness, porosity, opacity, double
folding, gloss, impurition, breaking length, resistance to feathering
Thread: Count, twist, strength, single,
Chemical composition (bs..): wool percentage , cotton percentage ,
polyester percentage , visco percentage , colour fastness to rubbing,
water formaldehyde, sack-as ns (poly woven bag), microscopic
analysis
Pashmina: Warp& weft, pashmina, average fibre diameter , total
percentage, wool, total count
polywoven bags: Bag size, side valve, weight of each bag, mesh, wt.
of laper in valve, gussetted & stitching, linear density, cartppm,
bursting strength
Shoes: Toe test, heel test, flexible test, bursting strength
Mechanical testing laboratory: tensile strength, bend test, rebend test,
bond test, size
Veterinary medicine : Hardness, fiability test, test of content of drug
per ml (albendazol, amoxycyline, proxacyline, paracetamol)
Veterinary Vaccine : Veterinary vaccine(potency test, safety test,
vacuum test, titer test, proficiency test), test for new strain
Feed: Aflatoxin (B1 and B2)
Milk and Meat: Antibiotic residue (qualitative test)
Meat, milk and eggs and their products: TPC, Coliform, Salmonella
Medicinal plants: Identification, Physio-chemical screening
parameters, plants bioactive compound (Alkaloids, Flavonoids,

5.
5

6.
6

Department of Plant
Resources,
Thapathali, Kathmandu

Email:

Chemical Laboratory,
Department of Mines
and Geology,
Lainchaour, Kathmandu

Tel: (01) 4413540

Email:
[email protected]
website:
http://www.dmg.nepal.gov.np

Website:
http://www.dop.gov.np

Medical Research
Tel:(01) 4781269, 4780227,
Laboratory, Department
4780572, 4780432
of Drug Administration, Email: [email protected]
Bijuli Bazaar, New
Baneswor, Kathmandu

Ms Padma Prajapati

Rock, Ore, Minerals, Coal,

Water and Geochemical
Samples, Soil

Mr Tirtha Shakya

Drugs (Capsule, liquid,

Ointment, Vaccine and
injection)

Ms Kanti Shrestha

Medicinal Plants, Water and

Waste water, Industrial
Chemicals (Solvents, Pectin,
PVC, Acetic Acid), Food,
Agrochemicals (Fertilizers,
Pesticides), Miscellaneous

Website:
http://www.dda.gov.np

7.
1

Natural Products
Research Laboratory,
Nepal Academy of
Science and Technology,
Khumaltar, Lalitpur

Tel: (01)5547714
e-mail: [email protected]
website:
http://www.nast.org.nop

Sapponins, Polysachharides, Curamins), taxol identification and

content determination
Pharmacological parameters: toxicity level identification, tests of
anti-fertility, anti-helminthes property, isolated tissue tests
Aromatic plants : Essential oil (qualitative and quantitative tests),
Physico-chemical parameters of essential oil
Plant standardization : Standardization of Nepalese plants,
Product formulation : Drug formulation-trial tests
Rock, Ore, Minerals: Moisture, Acid insoluble ash, Silica, Total
oxide, Calcium, Magnesium, Aluminum oxide, Loss of ignition.
Coal: Moisture, Acid insoluble matter, Fixed Carbon, Sulfur, Calorific
Value, Loss of ignition, Total oxide (calcium, magnesium, aluminum
and magnesium oxide)
Water: Minerals (Lead, Cupper, Zinc, Magnesium, Cadmium, Iron,
Manganese), pH, Ammonia, Conductivity, Total Hardness, Acidity,
Alkalinity, Chlorine and sulphate ions, TDS, TSS.
Geo-chemical samples : Minerals (Lead, Cupper, Zinc, Magnesium,
Cadmium, iron, Manganese)
Soil: Moisture, pH, Sodium, Potassium, Lead, Arsenic, Calcium,
Phosphorous, Phosphate, Sulfate, Nitrate ions
Capsule, liquid, Ointment and injection ( in dosage form): Active
ingredients in medicine, identification, assay, Content uniformity,
Pyrognene, LAL test, tests of sterility
Tablet: Dissolution and Disintegration
Tests of vaccine
Chemical analysis : Qualitative and quantitative tests (according to
pharmacopeia), Metal (sodium, potassium, calcium and iron)
Microbiological tests: Total plate count, E. coli, Salmonella,
coliforms, streptococcus
Bioassay of medicine
Medicinal plants: Essential oil, Bioactive compounds in
plants( Alkaloids, Flavonoids, Sapponins, Polysachharides, Curamins)
identification and characterization , taxol, Antibacterial, Antifungal
tests of medicinal plants, Cytotoxicity tests,
Water and Waste water: Color, Turbidity, Electrical conductivity,
Total solids, Total Dissolved solid, Total Suspended solids, Chlorides,
Fluoride, Sulphate, Sulphide, Nitrate, Ammonia, Silica, Nitrogen,
phosphorous, Total Hardness, Acidity, Carbonate, Bicarbonate,
Hydroxide, Phenolic Compounds, Manganese, Silica, Iron, Sodium,
Potassium, Lithium, Calcium, Magnesium, Copper, Zinc, Nickel, Lead,
Total Chromium, Lead, Arsenic, Cobalt, Mercury, Cadmium,
Aluminium, Selenium, Silver, BOD, COD, TPC, Coliform, Fecal
Coliform, Streptococcus

8.
1

9.
1

Food Research Unit,

Nepal Agriculture
Research Council,
Lalitpur

Tel: (01) 5544459

National Forensic
Laboratory,
G.P.O.
Box:4540,Khumaltar,
Lalitpur, Nepal

Tel: (01) 5526927/5553049

10. Nepal Environmental

and Scientific Services
7
(P.) Limited,
GPO Box 7301
Thapathali, Kathmandu,

Mr Mahendra Jung
Thapa

Food

Ms Vinushova
Tuladhar

Biological Samples (Semen,

Wild life products, Weapons,
Hair fiber)
Alcohol, Crime, DNA
Profile

Mr Pitambar Sharma

Water and Waste water,

Air Pollutants Cement
and Fly Ash, Fertilizer,
Soil, Food Products,
Spirit and Alcoholic
drinks, Pesticides, Coal
and Briquettes, Rock and
Minerals, Molasses,
Petroleum Products,
Noises

website:
http://www.narc.gov.np

website:
http://www.forensic.gov.np

Tel: (01) 4244989/4341001

e-mail:
[email protected]
website:
http://www.ness.com.np

Fertilizers :Nitrogen, potassium, Moisture, Phosphorus

Pesticides in food : 97 pesticides by GC-MS
Honey: Sucrose, Reducing sugar, Fructose glucose ratio, HMF and
pollens and enzyme analysis.
Explosive Analysis, Metallurgical Analysis;Azo-dyes, Methyl alcohol
Identification of compounds by GC-MS
Food grains: Grading (Foreign matters, organic and inorganic matters,
insect and heat damaged grains) of cereals and Pulses, Proximate ,
minerals (calcium and iron)
Tea : water extract, Alkalinity of soluble ash, Caffeine.
Wheat flour : Alcoholic acidity, gluten, Acidity of extracted fat
Biological samples: 97 Pesticides by GC-MS ( Insecticides and
rodenticide poisons, drugs of Abuse, Metallic poisons, Ethyl alcohol in
blood and urine, Methanol in blood, Cyanide in blood, Carbon monooxide in blood, Gun powder identification, Blood or saliva or semen
test, ABO Grouping and Testing, Diatom test, Morphological
comparison of skeleton, hair, fiber, Rhino horn test, Muskcone test,
Toxic chemical test- Acid/Alkali/Nitrite
On the spot crime scene examination.
DNA Profile
Water and Waste Water: Temperature, Odor, Taste, TSS, pH,
Conductivity, Redox potential, colour, TDS, TS, Discharge, Hardness,
NH3, NO2, TKN, CO2, Chloride, Floride, P, Sulphide, DO, SO4,
BOD, COD, Oil and Grease, Phenol, As, B, Ca, Mg, Ag, Trace Metals,
Larvae, Worms, Ova, Cyst, TPC, Total Coliform, E. coli, Yeast and
Moulds, Salmonella and Sighella, Vibrio Colera, Nitro Bacter
Air Pollutants (Particulate matter, Gaseous pollutants: TSP,
Thoratic Particulates (PM10), Lead, Benzene, Gaseous Pollutants (CO,
NOx, SO2, NH3, CO2, O3, H2S,)
Cement and Fly Ash: Moisture, LoI, Insoluble residue, SiO2, SO3,
Na2O,K2O, Soluble Alkali, Sillica, Tricalcium silicate, Dicalcium
Silicate, Tricalcium Aluminate, Tetracalcium Aluminum Ferrate, TiO 2,
P2O5, CaO, MgO, Free CaO, Inherent Chloride, Mixed Oxide
Sand and Aggregates: Mineral identification-such as % of Mica and
quantity alkali reactive silica, reduction in alkalinity, chloride, organic
impurity
Fertilizer: Moisture, Total Nitrogen, Amonical Nitrogen, Total
Phosphorus, Water Soluble Phosphorous, Potassium, Organic Matter
Soil: Moisture, pH, Conductivity, Texture, Total Nitrogen, Organic
Matter, Available K, Available P, CEC, EC (K, Ca, Mg, Na) available
Micronutrients (Cu, Mn, Fe, Zn) B, Cl, SO4
Food Products (Flour, Milk, Biscuit, Salt, Instant Noodle,
Vegetable Ghee and Oil, Honey): Moisture, pH, Calorific Value, sp

11. Soil Test (P) Limited,

GPO 2967, Battisputali
8
Kathmandu

Tel: (01) 4470551/4489287

e-mail:
[email protected]
website:
http://www.soiltest.com.np

Ms Saraswati Vaidha

Water and Waste water,

Food, Cement, Sand, Soil,
Fertilizers, Waste water, Air

gravity, total solid volatile matter, acid, Insoluble Ash, Fat, Protein,
Fibre, Acidity, Starch, Sugar, Gluten, metals (Fe, Na, Ca, Mg, Pb, As,
Cu, Zn) Extracted fat, SNF, FFA, Acidic Value, NaCl, Iodine, Water
insoluble residue, Total Nitrogen, Staphylococcus Aureus, Peroxide
value, reducing sugar, carbohydrate, TPC, Total coliform, E. Coli,
Yeast & Moulds
Spirit and Alcoholic drinks: pH, TDS, Ethyl alcohol, specific gravity,
total acidity, higher alcohol, volatile acid, ester, methane, aldehydes,
Furfural, metals (Cu, Fe, Zn, Na, Pb, As), P, SO2, Sugar, fixed acid,
tartaric acidity
Pesticides: Fervelerate, Cypermethirin, DDT, 2, 4, D-methyl
Parathion, Phorate, Melathion, Endosulphan, Deltamethrin, Methyl Dimethate, Phenitrothion, Metaxyl, Carberdazim, Macozeb, Quinolphos,
Chlorpyrifos, Monocrotophos etc.
Coal and Briquits: Moisture, Ash, Fats and Oils, Crudefibre N,
Carbohydrates, Total Acidity, Organic Carbon, OM, P
Rock and Minerals: LoI, Mineral Identification, Acid Insoluble,
R2O3, Fe2O3, Al2O3, Ca, Mg, SiO2, SO4, Metals (Fe, Al, Pb, Zn)
Molasses: Ash, Total Sugar, Reducing Sugar (Infermentable) Coliform,
TPC, Yeast and Moulds
Petroleum Products: Density, Kinematic Viscosity, Distillation
Parameters, Calorific Value, Sulfur, Ried Vapour Pressure, Cloud Point,
Pour Point, Ramsbottom Carbon Residue, Smoke Point, Flash Point,
Copper Corrosion, Lead, Ash
Noises : Equivalent Sound Pressure Level, Sound Percentile Level,
Day- night Average Sound Pressure Level
Lime stone: Calcium, Magnesium, Loss of ignition, iron, Aluminum,
Total minerals
Food: Proximate analysis, Calcium, Iron, Vitamin C,
Oil content, Pesticides (DDT, BHC), Mycotoxin
Air: Air particulate, Sulphate and Nitrite ions
Fertilizers: Nutrients analysis(N, P, K), Micro-nutrients (Boron ,
Sulfur)
Sand: Mica, Deleterious matters
Cement: L.S.F., C.A., SO3
Aggregate: Alkali reactivity, Deleterious matter
Minerals: Lime stone, Magnesite, Hematide, Galena, Calcium,
Magnesium
Plant Materials: Total fat
Water and Waste water: pH, Hardness, TDS, Turbidity,
Conductivity.: pH, Total solid, BOD, COD, DO, Minerals (Calcium,
Magnesium, Iron, Chlorine, Cupper, Arsenic), TPC, Coliform, Fecal
Coliform, E. Coli.

12. Cemat Water Lab ( P)

Limited,
9
Bijuli Bazaar,
Kathmandu, Nepal

Tel: (01) 4780451

13. Water Engineering and

Training Center,
1
GPO 8975, EPC 5205
Dillibazaar, Kathmandu
Nepal

(01) 4418156
Email:
[email protected]
[email protected]

Ms Keshari
Bajracharya

Water and Waste water,

Alcohol, Food

Mr Bhola Nath Paudel

Meat and Meat Products,

Fruit Juice, Milk, Tea and
Coffee, Water and waste
water, Cement

Mr K B Sahi

Food, Water, Waste water,

Alcoholic Beverages,
Chemical Fertilizers, Coal,
Cement, Soil

e-mail:
[email protected]

website:
www.wetcpl.com

14. Vishwa Laboratory and

Research Service,
1
Indrayani,
Balkhu,Lalitpur

Tel: (01) 4433748

e-mail:
[email protected]

Water and Waste Water: Temperature, Odor, Taste, TSS, pH,

Conductivity, Redox potential, colour, TDS, TS, Discharge, Hardness,
NH3, NO2, TKN, CO2, Chloride, Floride, P, Sulphide, DO, SO4,
BOD, COD, Oil and Grease, Phenol, As, B, Ca, Mg, Ag, Trace Metals,
Larvae, Worms, Ova, Cyst, TPC, Total Coliform, E. coli, Yeast and
Moulds, Salmonella and Sighella, Vibrio Colera, Nitro Bacter
Alcohol: pH, TDS, Ethyl alcohol, specific gravity, total acidity, higher
alcohol, volatile acid, ester, methane, aldehydes, Furfural, metals (Cu,
Fe, Zn, Na, Pb, As), P, SO2, Sugar, fixed acid, tartaric acidity
Food: Proximate, TPC, Coliform, Salmonella, Yeast and mold counts.
Meat and meat products, fruit juice: TPC, Coliform, Salmonella,
Shigella, V. Cholera, Yeast and mold counts, Parasites.
Food: Proximate analysis, Pesticide residues (97 pesticides including
DDT, Aldrin, Dialdrin, Malathion, BHC)
Water and Waste water:: Odor, Taste, TSS, pH, Conductivity, Redox
potential, colour, TDS, TS, Discharge, Hardness, NH3, NO2, TKN,
CO2, Chloride, Floride, P, Sulphide, DO, SO4, BOD, COD, As, B, Ca,
Mg, Ag, Trace Metals, Larvae, Worms, Ova, Cyst, TPC, Total
Coliform, E. coli, Yeast and Moulds, Salmonella and Sighella, Vibrio
Colera, Nitro Bacter, Metal content ionic parameters (Chlorine, nitrate,
phosphate, hardness)
Microbiological tests in food and water: TPC, Coliform, Salmonella,
Shigella, V. Cholera, Yeast and mold counts, Parasites.
Tea and coffee: Crude fiber
Milk and milk products; Fat , SNF
Soil :N, P, K and Micronutrients (boron, Cu Zn)
Cement : Calcium
Food: Proximate analysis
Noodles : Proximate analysis including MSG
Fats/oil: Acid value, iodine value, peroxide value
Water and Waste water: Minerals such as Arsenic, Cadmium,
Mercury, Zinc, Lead, Copper, , Iron, Magnesium, TDS, pH, Hardness,
and alkalinity, Ions (Ammonium, Nitrate, Nitrite, Phosphate, Ferrous)
TPC, Coliform, Salmonella, Yeast and mold counts: pH, Conductivity,
Hardness, Turbidity, Calcium, Magnesium, Nitrate, Nitrite, Phosphate,
DO, BOD, COD, Nitrogen, Lead, Cadmium, Mercury, Chromium,
Arsenic.
Soil: N, P, K, Micronutrients(Boron, Zinc), texture, organic matter
Chemical fertilizer: N, P, K, water soluble anions
Milk and Milk products : Fat, Protein, SNF
Alcoholic beverages: Alcohol strength, solid content, methanol, higher
alcohols, ester, fusel oil, pH, acidity
Lime stone: CaO, MgO, Silica carbonate, iron oxide, Aluminum oxide,

15. Water Analysis Services

Dilli Bazaar, Kathmandu
1

Tel:(01) 4433368
e-mail:
[email protected]
m

Mr Upendra B
Shrestha

Water and Waste Water

16. Nepal Research

Foundation P Ltd,
1
Om Ashram
Sreepur, Birjung
Birgunj

Tel: (051) 521097/ 528689

e-mail:
[email protected]
[email protected]

Mr Mahendra B
Amatya

Pharmaceuticals (Allopathy,
Ayurvedic), Food, Water and
Waste water

17. Environment and Public

Health Organization
1
( ENPHO)
GPO Box 4102
110/25 Adarsha marg,
Naya Baneswor,
Kathmandu

Tel: 01-4468641/4493188
Email:
[email protected]

Mr Amresh P
Karmacharya

Water and waste water, Solid

waste, Soil, Compost and
Dust, Air Quality

website:
http://www.enpho.org

Loss of ignition, Insoluble

Cement: CaO, MgO, Sodium and iron oxide, insoluble, sulfate, Silica
Coal: Calorific value, ash, volatile matter, carbon, sulfur
Water and Waste Water: pH, Hardness, TDS, Turbidity,
Conductivity. BOD, COD, DO, Minerals ( Calcium, Magnesium, Iron,
Chlorine, Cupper, Arsenic)
Microbiological: TPC, Coliform, Fecal Coliform, E. Coli, worms,
Larva, Geardia.
Allopathy and Ayurvedic Medicine: Histopathological &
Biochemical parameters
Hematology: Complete Blood Count (CBC) with no Differential
(WBC, RBC, HCT, HGB, MCV, MCH, MCHC, MPV, RDW, CHCM),
Platelet (automated count), Reticulocytes, Differential Count,
Complete Blood Count (CBC) with Differential
Clinical Parameters: Alanine Aminotransferase, Albumin, Alkaline
Phosphatase, Aspartate Aminotrasferase, Calcium Chloride,
Cholesterol, Creatinine, Glucose, Prealbumin, A/G/ Ratio, Globulin,
Phosphorous, Potassium, Sodium, Total Bilirubin, Total Protein,
Triglycerides, Urea, Nitrogen, Osmolality, Anion Gap
Advanced Lipid biomarkers: HDL-C, LDL-C, VLDL, Cardiac risk
ratio, Apo A1, Apo B, FFA
Cardiac biomarkers: BNP, Troponin I, Myoglobin, CK, CK-MB,
Homocysteine, Hs-CRP, D-Dimer
Food, Water and Waste water: Proximate, Color, Turbidity, Electrical
conductivity, Total solids, Total Dissolved solid, Total Suspended
solids, Chlorides, Fluoride, sulphate, sulphide, Nitrate, Ammonia,
Silica, Nitrogen, phosphorous, Total Hardness, Acidity, Carbonate,
Bicarbonate, Hydroxide, Phenolic Compounds, Manganese, Silica,
Iron, Sodium, Potassium, Lithium, Calcium, Magnesium, Copper,
Zinc, Nickel, Lead, Total Chromium, Lead, Arsenic, Cobalt, Mercury,
Cadmium, Aluminium, Selenium, Silver, BOD, COD
Microbiological: TPC, Coliform, Fecal Coliform, Streptococcus
Water and waste water: Inorganic, Organic , Pesticides , Heavy
metals, Bacteriological , Ammonia test Kit, Arsenic field test kit,
Chloride test kit, Dissolved Oxygen test kit (DO), Coliform
Presence / Absence test (vial), Free Residual Chlorine test kit
(FRC), Iron test kit, Hardness Test Kit, Nitrate test kit, Phosphate
test kit, Water Test kit
Solid waste, Soil, Compost and Dust: Inorganic, Organic,
Pesticides ,Heavy metals
Air: Particulate Chemistry, Emission Chemistry
Noise: Ambient Environment , Working Environment

18. SEAM-N
Environmental
1
Laboratory,
Morang Merchant
Association
Goshwara Road,
Biratnagar, Nepal

Tel: 021-527173 / 523915

Email:
[email protected]
Website:
http://www.mma.org.np/mma/sea
m-n-lab

Mr Bikrant Bikram
Chand

Water and Waste water, Food

Water and Waste water : Color, Turbidity, Electrical conductivity,

Total solids, Total Dissolved solid, Total Suspended solids, Chlorides,
Fluoride, sulphate, sulphide, Nitrate, Ammonia, Silica, Nitrogen,
phosphorous, Total Hardness, Acidity, Carbonate, Bicarbonate,
Hydroxide, Phenolic Compounds, Manganese, Silica, Iron, Sodium,
Potassium, Lithium, Calcium, Magnesium, Copper, Zinc, Nickel, Lead,
Total Chromium, Lead, Arsenic, Cobalt, Mercury, Cadmium,
Aluminium, Selenium, Silver, BOD, COD, TPC, Coliform, Fecal
Coliform, Streptococcus
Food: Proximate, TPC, Coliform, Fecal Coliform, Streptococcus

Annex 4: Human Resource in Laboratories under survey

SN
1

Name and address

Central Food Laboratory, Department of Food
Technology and Quality Control , Kathmandu
Nepal Bureau of Standard and Meteorology,
Kathmandu,
Veterinary Standards and Drug Administration,
Kathmandu
Natural Products Research Laboratory,
Department of Plant Resources, Kathmandu
Chemical Laboratory, Department of Mines and
Geology, Kathmandu
Drug Research Laboratory, Department of Drug
Administration, Kathmandu
Natural Products Research Laboratory, Nepal

Academy of Science and Technology, Lalitpur

Food Research Unit, Nepal Agriculture

2
3
4
5
6

9
10
11
12
13
14
15
16
17
18

Research Council, Lalitpur

National Forensic Laboratory, Lalitpur
Nepal Environmental and Scientific Services
(P.) Limited, Kathmandu,
Soil Test (P) Limited, Kathmandu
Cemat Pvt. Limited, Kathmandu
Water Engineering and Training Center,
Kathmandu
Vishwa Laboratory and Research Service,
Kathmandu
Water Analysis Services , Kathmandu
Nepal Research Foundation P Ltd, Birgunj
Environment and Public Health Organization,
Kathmandu
SEAM/N Laboratory, Biratnagar

Human Resource (with capability in analysis work )

14 (4 chemists, 4 food technologists 2 botanists, 2
microbiologists, 2 others in biological sciences)
3 (2 food technologists, 1 chemist)
3 (1 virology, 1 molecular biology and 1 agriculture
research and development)
5( 2 chemists, 1 pharmacist and 2 botanist)
7 (5 chemists and 2 others in biological sciences)
14 (8 chemists and 6 pharmacists)
6(5 chemists and 1 microbiologist)
3 ( 3 food technologists)
15 ( 4 chemists and others with DNA specialization )
3(2 chemists and 1 microbiologists)
4 (3 chemist and 1 microbiologist)
2 (1 chemist and 1 microbiologist)
7 (5 chemist and 2 microbiologist)
3(2 chemists and 1 microbiologist)
4(2 chemist and 2 microbiologist)
12(8 chemists, 3 pharmacists and 1 microbiologist)
3 (1 chemist, 1 microbiologist and one environmental
expert)
4 (1 chemist, 1 microbiologist and 1 environmental
expert and 1 food technologist)

Annex-5: List of Equipments and its Status in Laboratories

Annex 6: Laboratories with their Quality Management System and Related Information
Annex 7: Accredited Laboratories under NEPLAS scheme
1.
2.
3.
4.
5.
6.
7.

Environment and Public Health Organization, Kathmandu Nepal

Nepal Environmental and Scientific Services (P.) Limited, Kathmandu, Nepal
Soil Test (P) Limited, Kathmandu
Cemat Pvt. Limited, Kathmandu
SEAM/N Laboratory, Biratnagar
Water Engineering Laboratory, Kathmandu
Multi Pharmaceutical Laboratory, Lalitpur

Annex 8: Participation in PT by different Laboratories

Annex 9: Practice of Internal Audit, Complaint handling, Preventive action and Management review and Laboratory
safety status
Annex 10: Accommodation and Environment of the Laboratories
Annex 11: Test facility in different laboratories with HPLC, GC, AAS : Conditions and Test Parameters