Bioassay Procedures: Dod 51 Oo.52-M

DoD 51 OO.
52-M
CHAPTER 8
BIOASSAY PROCEDURES
8-1 BIOASSAY
(2) Urine Sampling. Urine sampling is a less

sensitive indicator of plutonium exposure; only a tiny
fraction of the amount inhaled is excreted through urine.
This fraction also depends on volubility of the plutonium
in the original aerosol. Samples taken during the first
five days after the exposure will not reflect the quantity
of plutonium inhaled due to the time requir:d for
movement through the body. Urine samples taken up
to 200 days after the exposure can be used for analysis.
Urine samples must be processed in a chemistry
laboratory before quantification is possible, but
screening for very high levels can be done in the field.
Samples should be submitted in plastic or glass bottles
with well-sealed tops.
(3) Lung Counting. Lung counting is the direct
measurement of emitted x-rays and gamma radiation
from the body with a sensitive low energy photon
detector. Probably, lung counting is the most accurate
method of determining internal exposure. Lung counters
are used at National Laboratories, commercially, and
at some hospitals and universities. Most lung counters
are immobile systems using large shielded rooms (special
trailer mounted systems can be obtained through the
Department of Energy (DoE) in a few days), and the
patient must be sent to the facility. Plutonium is retained
in the lung for a very long time.
a. Bioassays are procedures which estimate the

amount of radioactive material deposited in the body,
either by direct measurement, using sensitive x-ray
detectors placed over the chest (lung counting) and/ or
other organs, or by detection of radioactivity in the
excreta (feces and urine). Therefore, a number of factors
must be known in addition to the quantity and isotopic
distribution of the material to make an accurate estimate
of the dose.
Chemical form
Route of intake
Elapsed time from intake
Organ(s) containing the material
Distribution pattern
Organ(s) mass(es)
Biological half-life
Particle size of the original material
Decay scheme of the radioisotope
Complex mathematical models have been developed that
take each of these into account.
b. Three methods are used to determine the amount
of material present in the body. Each method has specific
advantages and disadvantages and the specific methods
in any given situation will be determined by the health
physicists.
8-2
BIOASSAY PROCEDURES
(1) Fecal Sampling. Fecal sampling is an effective

bioassay method which has the advantage that samples
a. Administration of a bioassay program for effected
can be evaluated in the field. Samples should not be
civilians may be the responsibility of the state or Federal
agency or effected country. The guidelines in Table 8taken until at least 48 hours after exposure to permit
passage of the contamination through the intestinal tract
1 are provided to assist the response force or civiiian
and should be submitted in well-sealed plastic bags. Low
authorities conducting initial screening in advising
energy gamma radiation sensors such as the FIDLER
individuals contaminated when requested to provide
can be used to estimate the plutonium content. For more
urine or fecal samples for analysis. Advisors explain that
definitive results, chemical separation and low level
sample analysis will determine if the individual received
counting techniques must be used. Fecal sampling is
a detectable radiation dose when contaminated. The
especially effective during the first few days, but can
bioassay procedures used will be established by health
be done at any time during the first year after exposure.
physicists responding to the accident. When bioassay
Figure 8-1 may be used to estimate the frst year dose
samples are collected, every effort should be made to
.commitment to the lungs based on contamination
keep samples and their containers free of contamination
detected in feces.
from the environment, clothing, or skin. Since tritium
8-1
FIRST YEAR DOSE COMMITMENT TO LUNG
r--- 1
FCCAL .wmrs, s
INTAKE ASSUMPTIONS
L.JEAPONS GRAOE P L U T O N I U M
Am-at IWJRITY 1200 pm.
PARTICLE SIZE 3.0 W-l AMAO
25X U VOLUBILITY CLASS
75X Y VOLUBILITY CLASS
OTHER ASSUMPTIONS
ICRP-30 METABOLIC MOOEL
DOSE CALCULATED FOR MINIMUM
DETECTABLE LEVEL (MDLI fN BIOASSAY
MOL FOR URINE ANALYS[S = O 095 PC1 /SAMPLE
MOL FOR FECAL ANALYSIS = O 95 PCI/SAMPLE
MOL FOR IN-VIVO LUNG COUNT = 500 PC I AM-2+1
OUAL 1 TY FACTOR FOR ALPHA = ?0
15
10
T
ME
OF
20
25
30
35
40
95
50
S A M P L E (DAYS A F T E R I N T A K E )
Use ofChart.
1. Enter chart with the time ofsample.
2. Draw vertical line tocumefor sample type.
3. Draw horizontal iineto Dose Equivalent scale.
4. Divide thevalueobtained from theDose Equivalent scalebytheMDL for the sample type and multipiy this vahreby the
contamination level of thesrsmple inpicocuries to get theestimatedfust year dose to the lung.
Example: Measurement ofafecd nmpletaken 5daystiter theaccident read lOOO~i.

1. Steps 1-3 give a Dose Equivalent Scale value of 4 x 10 .
-4
2.
3.
TheMDLforfecal samplesis.45pCi.
4 x 10-4
x 1000= .889 rem (The estimated fust year dose to the lung.)
.
4
5
Andy@ ofawmple taken from themmeperson (mmefirst yeadow) 10daysafter theaccident would beexpected to read only
20 ~i, assuming the individual inhafed contamination only on the day of the accident.
.-
Figure 8-1. Estimated First-Year Dose Commitment to the Lungs.
TABLE 8-1. Guidelines for Bioassay Sampling.

Suspected
Radioactive
Material
Plutonium
Uranium
Tritium
Feces
Optimum Sampling
Time After
Exposure
Urine
Optimum Sampling
Time After
Exposure
2 days
2-3 weeks
24 hours
4-8 hours
2 days
N/A
contamination cannot be detected by CCS monitoring,

anyone suspected of having been exposed to tritium
should follow the guidelines in Table 8-1. A bioassay
program is recommended for all individuals without
respiratory protection and found to be contaminated.
This program will determine if any dose was received
and provides assurance to those who did not receive
a dose that their health was not effected. To provide
similar assurance to all people in the contaminated area,
bioassays may be appropriate even for people who
werent found to be contaminated; moreover, some
people never in contaminated areas will request tests
to ensure they were not effected by the accident.
Sample
@.?!@l!
24 hours total
24 hours total
1 voiding .
results of both alpha meter screening and bioassays. Use

of the Radiation Health History and Bioassay Screening
Forms contained in Appendix 5-E should be considered.
(2) Nasal Smears. If initial alpha meter screening
indicates probable plutonium inhalation, a nasal smear

shall be collected for analysis by specialized teams when
they arrive on-site. Contamination on a wipe (Q-Tip)
from inside the nasal passage is a possible indicator of
plutonium inhalation. Due to the biological half-life of
nasal mucus, a nasal smear is a reliable indicator only
if collected during the first hour after t he exposure. When
medical personnel collect nasal smears, the Q-tip must
be free from any gels or other material that will prohibit
alpha particle counting.
(1) Bioassay Priorities. If a nuclear weapon accident

occurs near a populated area, obtaining bioassay samples
from large numbers of people may be necessary.
b. Personnel Exposure and Bioassay Records.

Documentation should be maintained on all personnel
who enter the radiological control area, or who may
have been contaminated prior to establishment of a
radiological control area. Examples of forms used for
recording data on personnel working in the radiological
control area, or who may have been exposed to
contamination downwind from the accident, are
contained in Appendix 5-E. To ensure appropriate
follow-up actions are completed on all exposed, or
potentially exposed people, a copy of all CCS logs, other
processing station records, bioassay data, and other
documentation identifying people who were or were not
contaminated should be provided to the Joint Hazard
Evaluation Center for consolidation into a single data
file. This data file is subject to Privacy Act regulations,
and must be retained as part of the permanent accident
records. Therefore, procedures for handling data
obtained on non-DoD personnel should be coordinated
with the OSCS legal officer. Data obtained on DoD
personnel will be needed to satisfy Service-specific
requirements contained in AR 40-14, Control and
Recording Procedures for Occupational Exposure to
Ionizing Radiation; NAVMED P-5055, Radiation
Health Protection Manual; AFR 161-8, Control and
Recording Procedures Occupational Exposure to
Ionizing Radiation; AFR 161-28, Personnel Dosimetry
Program and the USAF Master Radiation Exposure
Registry, references (q), (r), (s), and (t). These records
- shall be retained and become part of the individuals
permanent medical record.
NOTE: Since it is virtually impossible for a significant

amount of plutonium to be incorporated into the body
without gross contamination of skin or clothing also
occurring, initial alpha monitoring which identifies
contaminated personnel also can provide a method for
assuring that those with the greatest possibility of
radiation exposures which may affect their health are
given priority treatment.
Table 8-2, applicable only to people not wearing
respiratory protection, provides recommended guidelines for the assignment of priorities for bioassay
analysis. Response force personnel will normally be
equipped with protective clothing and respirators, when
required. Bioassays for response force personnel will be
performed in accordance with Service regulations and
as directed by the On-Scene Commander.
Personnel falling in the HI priority category in Table
8-2 may have had a substantial plutonium intake.
Conversely, exposure to airborne contamination which
produces a surface contamination level in the LO
category will be less likely to result in a significant
deposition in the lungs, To ensure alpha meter readings
provide a valid guide for assignment of priorities,
individuals should be asked, during screening, if they
have bathed or changed clothes since the time of possible
contamination. A record must be made and retained
for future reference of all personnel screened and the
8-3
TABLE 8-2. Guidelines for Assignment of Priorities for Collection and Processing of Bioassays.
Alpha Contamination Level on Clothing or Skin
E!@.@
60 cmz probe
17 cmz probe
HI
Above 300,000 Cpm

50,000-300,000 cpm
Above 75,000 Cpm

12,500-75,000 cpm
Below 12,500 cpm
MED
LO
Below 50,000 cpm
8-4

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DoD 51 OO.

(2) Urine Sampling. Urine sampling is a less

a. Bioassays are procedures which estimate the

(1) Fecal Sampling. Fecal sampling is an effective

FIRST YEAR DOSE COMMITMENT TO LUNG

Example: Measurement ofafecd nmpletaken 5daystiter theaccident read lOOO~i.

Figure 8-1. Estimated First-Year Dose Commitment to the Lungs.

TABLE 8-1. Guidelines for Bioassay Sampling.

contamination cannot be detected by CCS monitoring,

results of both alpha meter screening and bioassays. Use

indicates probable plutonium inhalation, a nasal smear

(1) Bioassay Priorities. If a nuclear weapon accident

b. Personnel Exposure and Bioassay Records.

NOTE: Since it is virtually impossible for a significant

Above 300,000 Cpm

Above 75,000 Cpm

Below 50,000 cpm

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