This SHEL LAB Product is Manufactured by

Sheldon Manufacturing Inc.

PO Box 627 Cornelius, OR 97113
Toll Free: (800)322-4897 Phone: (503)640-3000 Fax: (503)640-1366
email: [email protected]
web site: www.shellab.com

The SHEL LAB

High Heat Decontamination
CO2 Incubator

Installation & Operation Manual

This Manual Applies to

the Following Models:

3552
3552-2
High Heat
Decontamination
CO2 Incubators

Page 1

Table of Contents
Table of Contents

Section

Subject

1
2
3
4
5
6
7
8
9
10
11
12
13
14
15

Important Manufacturer Recommendations

CO2 Incubator Overview
Receiving and Inspecting Your CO2 Incubator
Graphic Symbols Glossary
Installation of your CO2 Incubator
Control Panel Overview
Operating Your SHEL LAB CO2 Incubator
CO2 Supply and Control System
Monitoring of CO2 Levels
Decontamination Cycle Operation
CO2 Incubator Maintenance Instructions
Trouble Shooting
Replacement Parts
Unit Specifications
Wiring Diagram

SHELDON MFG INC.

300 N. 26th Ave. Cornelius, OR 97113

1-800-322-4897 503-640-3000 Fax 503-640-1366

Visit our web site at www.shellab.com

CO2 Incubators Vacuum Ovens Water Baths Incubators Ovens

Low Temperature Incubators Humidity Test Chambers Shaking Incubators
Bactron Anaerobic Chambers Hybridization Ovens Shaking Water Baths

Manual part number: 4861611

Revised: 04/2010
Page 2

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SECTION

Important Safety Information

THANK YOU for choosing a SHEL LAB CO2 Incubator. This product is intended for professional, industrial or educational use
where the preparation or testing of materials is done at approximately atmospheric pressure.
Ensure all operators are trained before using this product and keep this manual where all operators can refer to it.
WARNING: For continued safe operation, read this manual before using the product and follow the basic safety
precautions below. Failure to do so could result in serious injury or death.
Follow any city, county, or other ordinances regarding the use of this product.
Use the unit only as described in this manual.
Use only approved accessories.
Plug the units power cord into a grounded electrical outlet that conforms to national and local electrical
codes.
Do not modify system components. Any alterations or modifications to the product may be hazardous and
will void your warranty.
Do not connect the unit to a power source of any other voltage or frequency beyond the range stated on the
power rating overlay on the dataplate inside the door of the unit.
Do no damage the power cord. A damaged cord can become a shock or fire hazard. Never use a power cord
after it has become damaged.
Do not position the equipment in a manner that makes disconnecting the power cord or coupler difficult.
Do not attempt to move the unit while in operation or before the unit has been allowed to cool.
Do not use with flammable, volatile, or combustible materials.
Do not use in hazardous or household locations.

Page 3

SECTION

SHEL LAB CO2 Incubator

Overview
Return to Table of Contents

Quick Links
Receiving & Inspection

Installation Details

Complete Control
Panel Overview

Welcome! Thank You for Purchasing a SHEL LAB CO2 Incubator

This Page is Meant to Give an Overview of the Features Built Into Your New CO2 Incubator.

Over Temperature Protection

Safety Control
Over Temperature Activated
Light

Access Port Stopper

Temperature Control
& Display

Alarm Contacts Relay

CO2 Sample Port
USB Port
Power Switch
Decontamination Cycle Control
& Display
Stainless Steel Shelving
Humidity Pan

Sheldon Manufacturing Inc.

P.O. Box 627
Cornelius, Oregon 97113
EMAIL: [email protected] WEB SITE: http://www.Shellab.com
PHONE: 1-800-322-4897 (503) 640-3000 FAX: (503) 640-1366

Page 4

CO2 Control & Display

(comes with unit)

Access Port to
Chamber
(through side of
unit)

Data Plate
Containing
Serial #

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Receiving & Inspecting

Your SHEL LAB CO2 Incubator

Receiving Your SHEL LAB Unit

All units are packaged with high-quality shipping materials to provide protection from transportation related damage.
However, once a unit leaves the factory safe delivery is the responsibility of the carrier who is liable for loss or damage to
your unit. Damage sustained during transit is not covered under your unit warranty.
Carefully inspect the shipping carton for damage. If the carton is damaged, report the damage to the carrier service that
delivered the unit.
If the carton is not damaged, open the carton and remove its contents.

Inspection Guidelines
When you receive your unit, inspect it for concealed loss or damage to its interior and exterior. Should you find any
damage to the unit, follow the carriers procedure for claiming damage or loss.
Verify that all of the following equipment is included in the crate using the: See Ship Kit Details Next Page (Page 6)
Save the shipping carton until you are sure everything is in order.

Returning Shipment
If you must return the unit for any reason, first contact your service representative for authorization. You will be asked
to provide the data plate information.

Recording Data Plate Information

Once you have determined the unit is free from damage, locate the data plate inside the door of the unit. The data plate
indicates your units model number and serial number. Record this information for future reference.

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Receiving & Inspecting

Continued

Ship Kit & Accessories Detail

Verify all items listed on this page are received while unpacking your unit.

5121109

5170646

7930514

1800510

5121028

2700506

9710500

7750506

Part Number

Description

Quantity

5121109
5170646
5121028
7750506

Stainless Steel Shelving

Stainless Steel Shelf Standards
Stainless Steel Shelf Slides
Access Port Stopper

3
4
6
1

2700506
7930514
9710500

Leveling Feet
Stainless Steel Humidity Pan
CO2 Tubing Kit: Includes Black Tubing, Clear Tubing, &
CO2 Disc HEPA Filters (Shown Above 100199)
Power Cord Set

4
1
1

1800510 (110V)
1800500 (220V)

To be sure that your accessories kit is complete, check inside packaging materials for loose items
and always keep them with the unit.
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Graphic Symbols Glossary

Graphic Symbols Glossary

Indicates Safety Alert. Potential personal injury hazard.

Indicates Temperature
Indicates Over-temperature safety
Indicates Degrees Celsius
Indicates Carbon Dioxide
Indicates Gas (Carbon Dioxide for the unit)
Indicates AC Power
Indicates ON / I and OFF / O

%RH

Indicates Percentage Relative Humidity

Indicates Relative Humidity

Indicates Protective Earth to Ground

Indicates Potential Shock Hazard behind Partition

Indicates Unit Should Be Recycled Do Not Throw In Land Fill

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Installation of Your CO2 Incubator

NOTE: The recommended operating environment for this equipment is 5 - 35C with a Relative Humidity of less than
80%. Supply voltage should not vary more than 10% from the data plate rating. Contact customer service for operating
conditions outside of these limits.

Power Source
The unit power requirements are listed on the data plate. Check the data plate for voltage, cycle, and ampere
requirements. Plug the power cord into a PROPERLY GROUNDED (earthed) Power source OF THE CORRECT SIZE AND STYLE
INDICATED ON THE UNIT NAMEPLATE RATING. A separate circuit is recommended for this unit to prevent loss of product
due to overloading or circuit failures caused by other equipment. The supply voltage must match the nameplate voltage
within +/- 10%. The US market units are designed for 120 VAC @ 60 Hz, 12.0 Amps. The EU market units are designed for
230 VAC @ 50/60Hz, 6.0 Amps. If supplied with a detachable cord set, plug the female end into the inlet on the unit and
the male plug into the supply. Assure that units requiring fuses have them installed. The fuse is a T type, 12.5 Amp for
110 volt units, 10 Amp for 220 volt units. This fuse may be at the inlet or a part of the cord set male plug.

Location
In selecting a location, consider all conditions that might affect performance, such as heat from radiators, ovens,
autoclaves, etc. Avoid direct sun, fast-moving air currents, heating and cooling ducts, and high traffic areas. Allow a
minimum of 10 cm between the unit and any walls or partitions that might obstruct free airflow and to promote easy
access to the power cord and source. Under normal circumstances this unit is intended for use indoors.

Lifting and Handling

CAUTION: Use appropriate lifting devices that are sufficiently rated for these loads. Failure to do so could result in
minor or moderate injury.
Units should only be lifted from their bottom surfaces. Handles and knobs are not adequate for lifting or stabilization.
The unit should be completely restrained from tipping during lifting or transport. All moving parts such as trays or covers
should be removed during transfer to prevent shifting and damage.

Leveling
The unit must sit level and solidly. Leveling feet are supplied and must be installed in the four holes in the bottom
corners of the unit. With the feet installed and the unit standing upright, each foot can be raised by turning it in a
counterclockwise direction. Adjust the foot at each corner until the unit stands level and solid without rocking. If the
unit must be moved, turn the leveling feet in (clockwise) all the way to prevent damage while moving.

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Installation Continued

Cleaning
Appropriate lab practice includes cleaning and disinfecting the unit prior to use. Your operating conditions and appropriate
protocol will determine the correct procedure for cleaning. A typical cleaning procedure is described below and will help
reduce the likelihood of contamination and the necessity of decontamination. Clean the unit regularly. Depending on usage
and protocol, this may be monthly or quarterly.
Cleaning the unit does not qualify the unit as decontaminated. Decontamination Cycle Operation is detailed in Section 10.
WARNING: Follow these safety precautions regardless of the cleaning procedure. Failure to do so may cause serious
injury or death.
Always disconnect the unit from the electrical service when cleaning.
Assure all volatile or flammable cleaners are evaporated and dry before reconnecting the unit to the power
supply.
NOTICE: Follow these precautions regardless of cleaning procedure. Failure to do so may cause property damage and
void your warranty.
Special care should be taken when cleaning around sensing heads to prevent damage.
Do not use chlorine-based bleaches or abrasive cleaners. These will modify the stainless steel interior finish.
Do not use hard tools such as metal wire brushes or steel wool. Use non-abrasive cleaners and soft tools such
as plastic brushes.

Typical CO2 Incubator Cleaning Procedure

1. Remove the humidity pan every week, wash with soap and water, and then disinfect with 70% alcohol solution. Replace
in the incubator with fresh, DISTILLED water.
2. Flush the sample port tubing with 70% alcohol solution. Replace any lines that have a green color.
3. Remove all shelves, shelf supports, shelf standards and shields. Wash and disinfect as described in item 1. For initial
set up, clean and disinfect shelving parts prior to installation. Refer to page 10 for detailed shelving installation.
4. Wash and disinfect all interior surfaces.
5. Give special attention to cleaning and disinfecting all access ports, air bleeds, shaft holes, electrical pass-through and
any other passages into the chamber.
6. Replace all air and CO2 filters every six months or when noticeably dirty on the upstream side. CO2 filters are located
in the shadow box just behind the GAS IN fitting and in line with the CO2 tubing kit and inside the pump box in the
shadow box.

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Installation Continued

Tips for Minimizing CO2 Incubator Contamination

Keep the outside of the incubator, including the air in the laboratory, as clean as possible.
Do not place incubators near doors, air vents or other areas of high air movement or traffic.
The floor around the CO2 Incubator should be kept as clean as possible.
Units that are placed on the floor should be on a caster platform to make moving the unit easier during cleaning
and when accessing the back of the unit.
Minimize the number of times access is made to the chamber during normal operation.
Do not depend on the use of antibiotics to maintain uncontaminated conditions, as this is an inadequate technique
for decontamination.
Use aseptic techniques as described above for maintaining decontaminated conditions in the incubator

Installing Shelving, Humidity Pan and Stopper Assembly

Completely remove protective vinyl coating from shelving apparatus and disinfect prior to installation.
1

SHELVING

Install shelf slider by aligning with shelf standards at a

slight upward angle and set into a level position.

Page 10

Slide shelves into position

with bent ends facing up.

STOPPER

HUMIDITY

Install shelf standards

by aligning the keyhole with
the peg on the chamber wall
and sliding down.

Install humidity pan by

placing directly on the
chamber floor.

Install Acess Port Stopper immediately into access port to

chamber. The Stopper should remain there unless access to
chamber through that port is necessary.

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Control Panel Overview

CONTROL PANEL OVERVIEW

Set Over Temp Control

Over Temperature Protection

Safety Control
Over Temperature
Activated Light
Over
Temp

Alarm Output Jack

CO2 Sample Port

Alarm Contacts

CO2 Sample Port

USB

USB Port

Heating Activated Light

Set Temperature C
Heating Activated
High

Temperature &
Alarm Display

Mute
Low

CO2 Injecting Light

Current Operation
Indicator Lights

Set CO2%

Decontamination Cycle Switch

CO2 Injecting

Mute

Alarm Controls

Decon Acticated

CO2 & CO2

Alarm Display

Normal Operation

Operation Selecor Switch

180C Hight Heat Decontamination

Main Temperature
Control

Decon Cycle

High

Low

Power

Decontamination
Cycle Switch

CO2 S

ERIES

She don Ma nufac turing Inc.

Main CO2 Control

Operation Selector Switch

Power Switch I = on, O = off

See Next Page for Detailed Descriptions of Each Feature

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Control Panel Continued

65

CONTROL PANEL FEATURE DESCRIPTIONS (See Illustration on Page 11)

All controls are located on the front panel. Units with a detachable cord have a fused inlet located at the top
rear of the incubator. This inlet has a recessed male plug, fuse and an EMI filtering system designed to filter
out electrical interference. This inlet also prevents any internally generated interference from feeding out to the
power grid.
Power Switch: The I/O (ON/OFF) switch controls all of the power for the incubator and must be in the I/ON
position before any systems are operational. Both temperature and CO2 displays will illuminate when the power
switch is in the ON position.
Over Temperature Protection Safety Control (OTP): This is a hydraulic thermostat that is wired between the Main
Temperature Controller and the heating element and functions as an override control. During normal operation, if
at any time the Temperature Control fails in the ON position, and the temperature in the incubator rises above its
set point, the OTP is activated and maintains temperature at its own set point. Note that the HEATING indicator
will continue to function under the control of the Over Temperature Safety.
Over Temperature Activated Light: This pilot lamp is on whenever the Over Temperature Safety thermostat has
been activated and taken control of the heating element. During normal operating conditions this indicator
should never be on.
Alarm Output Jack: This is located on the left control panel. It allows a remote alarm to be connected to the
unit.
CO2 Sample Port: This is located on the front left side of control panel. A sample can be drawn to measure the
CO2 content in the chamber at this port.
USB Port: At the Technical support level, this is typically used as a computer connection port for
analysis and trouble shooting. Click here to download the data logging software available for this unit.
Main Temperature Control: Heating activated light will be lit whenever the element is on. This controller is
marked Set Temperature C and indicates the actual temperature within the chamber to 0.1C. The UP/
DOWN buttons are used for inputting the set point, calibrating the display, and muting or unmuting the audible
alarm. The HIGH and LOW alarm indicators will light whenever there is an alarm condition associated with the
temperature within the chamber. The MUTE indicator will light whenever the audible alarm has been deactivated.
CO2 Control: This controller is marked Set CO2 % and indicates the percentage of CO2 content within the
chamber to 0.1%. The UP/DOWN buttons are used to input the set point, calibrating the display, and muting or
unmuting the audible alarm. The HIGH and LOW alarm indicators will light whenever there is an alarm condition
associated with the CO2% within the chamber. The MUTE indicator will light whenever the audible alarm has been
deactivated.
CO2 Injecting Light: This pilot lamp is on when the CO2 controller is injecting CO2 into the chamber.

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Control Panel Continued

CONTROL PANEL FEATURE DESCRIPTIONS (continued)

Operation Selector Switch: This switch is located on the front panel at the right side. It is used to select
between normal incubation operation and decontamination cycle operation.
Normal Operation Indicator Light: This green colored light is located on the front panel beside the operation selector
switch. It indicates the unit is operating in the normal incubator mode.
Decon Cycle Switch: This is located on the right side of the control panel. It starts the high
temperature decontamination cycle. The cycle will not start until the operation selector switch is pushed placing the
unit in decontamination operation mode.
Decontamination Cycle Light: This amber colored light is located on the front panel beside the
decontamination cycle switch. It indicates when the unit is in the decontamination mode.
Decon Activated Light: This red colored light is between the normal operation indicator light and the decon cycle light.
It indicates the unit is actively running a decontamination cycle.
Alarm Controls and Display: The temperature control and the CO2 control are both equipped with visual and audible
alarms.
Temperature Alarms: The temperature control has alarm indicators for high and low conditions that are
activated whenever the actual temperature is 1C above or below the set point.
CO2 Alarms: The CO2 controller has alarm indicators for high and low alarm conditions that are activated whenever
the actual CO2% is 1% above or below the set point.
Audible Alarms: Both controls have audible alarms that are activated when either of the HIGH or LOW indicators is
activated.
Muting Audible Alarms: The audible alarms can be muted for a single alarm occurrence by pressing and holding down
EITHER the UP or DOWN button for several seconds until the alarm mutes. There is a built in delay of approximately 15
minutes on the occurrence of a LOW alarm. This time delay prevents the audible alarm from activating every time the
door is opened and the temperature and CO2 drops.

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SHEL LAB CO2 Incubator

Operation Instructions

75

NOTICE: To ensure the proper operation, regularly verify the accuracy of the temperature and CO2 controls
regularly. Temperature can be confirmed by using a thermometer inside of the chamber. CO2 levels can be
confirmed with the use of a Fyrite Gas Analyzer or other equipment such as a gas chromatograph. Failure to do
so may damage the unit and void your warranty. Contact a technical service representative for questions.
Operating the Controls: Refer to the Control Panel Overview on page 11 of this manual for a list of terms used.
Powering On the CO2 Incubator: Check power supply against unit plate and make sure they match. Be certain that the
fuse is installed in the power inlet of the unit. Start by attaching the cordset (supplied with the accessories) to the unit
inlet and the building power supply. Attach the female end of the cordset into the fused and recessed male plug on the
rear of the unit and the male plug end of the cordset into the building supply receptacle.
Turn the power switch to the I/ON position. Operating temperature must be set, achieved and calibrated before C02
connection and adjustments are made. Set the C02 control setpoint to OFF. To do this, press and release the down arrow
Button. The display will flash SP and then a number. While the number is flashing, press the down arrow button
and hold it down until the display is flashing OFF, then release the button. Then press the down arrow until the CO2
setpoint is OFF.
Muting Audible Alarms: Powering the unit on prior to calibration may activate the built in alarm system. To mute the
alarm hold EITHER the up or down button until the mute indicator activates.
Initial Control Settings: Each SHEL LAB CO2 Incubator comes preset from the factory at 37C and the CO2 set to off. You
should allow the incubator to heat up and stabilize for 24hrs. Turn the overtemperature control clockwise until it stops
so that it does not activate during the initial warm up.
Calibrating the Controls for Temperature or CO2:
1. Push and hold BOTH the UP and DOWN buttons simultaneously for five (5) seconds.

a. If you are calibrating temperature, the Temperature Display panel will read CO and then blink.

b. If you are calibrating CO2 Level, the CO2 will read CO and then blink.
2. While the display is blinking use the UP and DOWN buttons to adjust the display to match the actual condition in
the incubator chamber.
3. If no buttons are pressed within five (5) seconds the blinking will stop and the display will
revert to showing the process or actual parameter within the incubator chamber.

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Operation Continued

Setting the Temperature:

1. Push and release either the UP or DOWN button and the digital display will blink SP and then a number which is
the setpoint.
2. While the display is blinking the set point can be changed using the UP or DOWN buttons.
3. If no buttons are pressed within five (5) seconds the blinking will stop and the display will revert to showing the
process or actual parameter within the incubator chamber.
Checking Temperature Settings: Perform the calibration in the following order:
Main Temperature Calibration -> Setting the Temperature -> Setting Over Temperature Safety.
1. During main temperature calibration do not open the inner glass door for any reason. Open the outer heated door as
little as possible.
2. After the incubator has been running for 24 hours and the temperature display is stable at the set point, read
the temperature in the chamber using a reference thermometer. If necessary, recalibrate the display using the
instructions found on page 14.
3. After the main temperature display has stabilized again and maintained set point for several hours, check the actual
temperature again. If it does not match the display repeat the calibration.
4. After the Main Temperature is set and adjusted, the Overtemperature Safety Control needs
set.

to be

Setting the Over Temperature Safety Control Thermostat: This should be done AFTER the main temperature control is set
and the unit has stabilized. Raise the Set Point 1 degree above desired Set Point. After it has stabilized, turn the safety
knob counterclockwise until the OTP Light illuminates. Then, return the Set Point back to the desired Set Point. This sets
the Safety at 1C above Set Point.
Setting the CO2:
1. Push and release either the UP or DOWN button and the digital display will blink SP and then a number which is
the setpoint.
2. While the display is blinking the set point can be changed using the UP or DOWN buttons.
3. If no buttons are pressed within five (5) seconds the blinking will stop and the display will
revert to showing the process or actual parameter within the incubator chamber.

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Operation Continued

75

Humidity Levels in the Incubator: Humidification of the incubator chamber is achieved by evaporation of water from the
chamber humidity pan placed in the bottom of the incubator. By filling this stainless steel reservoir pan with DISTILLED
WATER ONLY and allowing this water supply to heat and evaporate, near saturation humidity is obtained.
NOTICE: Follow the precautions below. Failure to do so may damage the unit and void your warranty.
Do not use plastic, glass or other metals as substitute humidity pans. Only 300 series stainless steel is
acceptable for this reservoir pan.
Do not use corrosive chemicals including copper sulfate or chlorine in the pan or chamber as damage may
occur.
Use Distilled Water Only. DO NOT USE DEIONIZED WATER.
Be aware that the use of disinfecting chemicals in the pan can change the surface tension of the reservoir water thus
preventing evaporation and proper humidification of the chamber. Water in the pan should be changed and the pan
cleaned at least once a week to help control contamination and to maintain proper surface tension.
Temperature Monitoring: To insure that the incubator is operating at the desired temperature, an accurate temperature
indicator such as a certified reference thermometer should be placed in the incubator chamber.
Try to place the thermometer in the center of the chamber and spaced off the shelf (if the thermometer is touching the
shelf it may give an inaccurate reading).
Digital thermometers with a remote display are ideal because they can be read without opening the doors. Be sure it is
rated for operation in a 95% Relative Humidity Atmosphere.
TIP: If you do not have a digital thermometer with remote display, taping the thermometer down to a petri dish cover is
a method that raises it off the shelf and prevents it from rolling and hiding the scale from view.

Page 16

SECTION

CO2 Supply & Control System

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CO2 Supply System and Control System: The CO2 system is rated for pressures between 10 to 20 PSI which should never
be exceeded at any time.
CO2 Inlet (B)

Liner Inlet
Tube (C)

Filter

Regulator

Solenoid
Valve

Liner Outlet
Tube (D)

Restrictor
IR Sensor
OUT

Sample
Port Tube

Filter

Air Pump
IN

A = The CO2 Sample Port is located on the control panel on the front left of the incubator.
B = The CO2 Inlet is located on the back left corner of the incubator.
C = Liner Inlet Tube is located in the chabmer top in the front left corner.
D = Liner Outlet Tube is located in the chamber back in the lower right corner.

CO2
Sample
Port (A)

Connecting CO2 to the Incubator: The CO2 inlet fitting is located at the back of the unit near the top (see above
image). It is marked CO2 TO CHAMBER.
A supply hose with in-line CO2 filter (supplied with your accessories) connects from the fitting to a CO2 tank and
regulator.

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CO2 Supply & Control System

Continued

85

CO2 Regulator Considerations: Ensure that you are using a dual stage regulator.
It is highly recommended that a good quality 0-60 PSI output range DUAL STAGE pressure regulator be used on the
CO2 tank. The dual stage regulator will have two pressure gauges. The high pressure gauge (0-4000 PSI) will indicate
the pressure within the tank. The low pressure gauge (0-60 PSI) will indicate the output pressure on the supply hose
to the incubator. Single stage regulators do not provide as stable performance as dual stage.
Reading & Understanding Your CO2 Tank Levels: It is normal for the high pressure gauge on your regulator to start
out reading 800 to 1000 PSI with a full tank. The reading will drop from 800 to 500 PSI quickly and will stay there
for most of the duration of the tank. At the end of use, the pressure will drop quickly to zero to indicate that the
tank is completely empty. Pure CO2 is in a liquid state in the tank, and a constant vapor pressure is generated in
the tank above the liquid level. The CO2 is drawn off of the top as a gas. The same vapor pressure is maintained as
long as any liquid is left in the tank. When the last of the liquid has evaporated into gas then the pressure will drop
rapidly as the gas is drawn off.
NOTE: Pressure from the regulator to the incubator should be set at 15 PSI. Models with additional internal regulators
have pressure reduction to 5 PSI to prevent overshoot.
NOTICE: Only medical grade CO2 should be used in your incubator. Failure to do so may damage the unit and
void your warranty.
Manufacturers Recommendation for Monitoring CO2 Levels: CO2 sensors are factory calibrated and, under normal
circumstances, need no calibration. It is recommended that the accuracy of your CO2 control system be monitored by
measuring the actual CO2 concentration on a weekly basis with a Fyrite or other
measuring device. This should be done when the chamber has not been opened for an extended period of time, i.e.
after the weekend, and should only take 1-2 hours.
Setting the CO2 Control: Attach the supply hose from the CO2 tank to the incubator CO2 inlet fitting and turn on the
CO2 supply. Set the CO2 control to the desired set point using the up and down arrows (section 7 page 15). The CO2
level comes from the factory preset to OFF.
Adjusting CO2 Display: After the incubator has had several hours to stabilize at CO2 set point, measure the actual
CO2% with a Fyrite. If there is any difference between the Fyrite and the display, use the
procedure described in section 7 page 14 for calibrating CO2. See Section 9 Page 19 (next page) for Fyrite use and
instruction.
NOTE: When using the Fyrite, insure that gas is not being injected while the reading is being taken. Always change
the CO2 set point to 0.0 prior to taking the sample and change the set point back to the desired value after the use
of the Fyrite is finished.

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SECTION

Monitoring of CO2 Levels

95

Using a FYRITE to Measure CO2 Levels

A Bacharach FYRITE CO2 Gas Analyzer is recommended to measure CO2 concentrations in the incubator chamber. This
test instrument is not supplied with the incubator but is readily available from your dealer. Follow the instructions provided with each Fyrite instrument carefully to ensure correct and accurate readings.

Fyrite Quick Overview

1. Press button on top of Fyrite canister to release CO2 concentration. RELEASE BUTTON and tip canister to the side
to ensure all fluid is released from the top of the canister. Return canister to the upright position.
2. Loosen screw on slide scale and align top of fluid with zero on the scale. Tighten screw.
3. Connect hose and aspirator bulb to unit being tested. The sample port for connection is located on the control
panel.
4. Place the hose sampling cap directly over the plunger valve on top of the canister and depress firmly.
5. With button depressed, squeeze bulb 20 times. On the last squeeze and with bulb still deflated, release hose from
button.
6. Turn Fyrite canister upside down 3 times, each time allowing all fluid to flow to the opposite end of the canister.
7. Tilt canister to the 45 degree position to ensure all fluid has been released from top of canister. Return canister to
the upright position.
8. Read CO2 concentration in %.
WARNING: The fluid used inside this fyrite instrument is poisonous and corrosive. If taken internally could result
in serious injury or death. The fyrite indicator will come with a complete set of detailed instructions which
should be followed carefully. In the event of a spill or accidental body contact with the fyrite fluid, follow the
instructions on the refill bottle carefully.

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SECTION

Decontamination Cycle
Operation

5
10

Although this unit was run through a Decontamination Cycle after manufacturing, it is highly recommended that a
Decontamination Cycle be run prior to using the unit. After following the cleaning procedure detailed on page 9, and all
accessories have been cleaned and installed into the unit, begin the Decontamination Cycle using the following instructions:
1. To Activate Decontamination Cycle: Press and hold down the lower Operation Selector Switch until the green Normal
Operation Light goes out and the amber Decontamination Cycle Light starts to flash.
NOTE: If neither Decontamination Cycle Switch nor Operation Selector Switch is depressed within one (1) minute, the
amber Decon Cycle Light will go out and the green Normal Operation Light will come on.
2. To start the Decontamination Cycle, press and hold the Decontamination Cycle Button when the amber
Decontamination Cycle Light is flashing. You will hear a thump for the door locking. The Door will remain locked
until the unit has cooled down to 50C at the END of the Cool down phase noted below.
3. During the warm up to decontamination temperature the amber Decontamination Cycle Light and the red
Decontamination Activated Light will flash alternately.
4. At all times the Temperature Display will indicate the actual temperature within the incubator chamber. During
the Decontamination Cycle warm up, the CO2 display will read dEC for Decontamination Cycle until the Chamber
Temperature reaches 180C. Under normal operating conditions, the Decon Cycle warm up to 180C will take less
than 1 hours.
5. After the Chamber Temperature reaches 180C, it will be controlled there for two (2) hours during which the CO2
display will count down from 120 to 0 indicating the number of minutes remaining. Also, during the timed portion
of the cycle, the red Decon Activated light and the amber Decontamination cycle light will be continuously lit.
6. At the end of the two (2) hour 180C Decontamination Period, the cool down portion of the Decontamination Cycle
will start.
7. During cool down the following will occur: The CO2 Display will read CdN. The amber Decontamination
Cycle light and the green Normal Operation Light will flash alternately indicating that the Decon Cycle has been
successfully maintained at 180C for two (2) hours and the unit is cooling down to normal incubation temperature.
8. To go back into Normal Operation Mode, depress the lower Operation Selector Button and hold it down until the
amber Decontamination activated light stops flashing and the green Normal Operation Light stays on solid.
9. If at any time during the Decon Cycle you wish to abort the Decon Cycle, press and hold down simultaneously both
Operation Selector Buttons until the amber Decontamination Cycle Light stays on and the red Decontamination
Activated Light stays off. If temperature is above set point, alarm will sound. See section 5 page 13 for alarm
controls.
NOTE: DO NOT attempt to open door until deactivation is completed. Green flashing lights will indicate the door is
unlocked and you will hear a distinct thump.

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SECTION

Decontamination Cycle
Operation Continued
Return to Table of Contents

5
10

The following chart covers the various meanings of the light displays during the units current operating condition.

Unit Condition and Relative Light Display

CONDITION

CO2 DISPLAY

AMBER

RED

GREEN

Normal Operation

% CO2

OFF

OFF

ON

Decon Operation Cycle

Not Started

% CO2

FLASHING

OFF

OFF

Warming up to 180C

dEC

Alternately Flashing

Alternately Flashing

OFF

Decon Cycle Running at

180 C for 2 Hours

Countdown From
120 to 0 Min.

ON

ON

OFF

Cool Down to 50 C

Cdn

OFF

OFF

FLASHING

Decon Cycle
Successful

PAS

OFF

OFF

FLASHING

Decon Cycle
Manually Aborted

Ab

ON

OFF

OFF

Decon Cycle Failed

FL

ON

OFF

OFF

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SECTION

SHEL LAB CO2 Incubator

Maintenance Instructions

5
11

WARNING: Prior to any maintenance or service on this unit, disconnect the service cord from the power supply.
failure to do so could result in serious injury or death.
Chamber Cleaning: Cleaning and decontamination are recommended on a regular basis. To prepare the incubator for
cleaning remove all interior parts such as the shelf standards and false top. Please review the Cleaning procedures
described in the Installation section for detailed instructions.
Monitor CO2 Levels: Check CO2 supply periodically; dont let it run out. Automatic tank switches and empty tank alarms
are available from your dealer.
Keeping the CO2 flow system free of impurities: Inconsistent levels of CO2 control are usually traceable to the CO2
pressure regulator on the tank, impurities in the tank, or impurities in the solenoid valve. Replace the CO2 in-line
filters every six (6) months or when the filters have become noticeably dirty on the upstream side. There is a CO2 filter
attached to the tubing kit, and one inside the shadow box connected to the GAS-IN line.
Microprocessor Controls: There is no maintenance required on any controls. If the controls fail to operate as specified,
we recommend powering the unit down, waiting a 10 seconds and turning it back on. Contact customer service if this
doesnt resolve the issue.
Proper Storage of Your SHEL LAB CO2 Incubator: If the incubator is to be turned off for any length of time, dry the
chamber and humidity pan thoroughly and leave at room temperature. Failure to do this may cause the interior to
become contaminated. No adjustment to controls should be required when restarting the unit. If the unit is to be shut
down for transport, remove humidity pan and shelves, dry the chamber and disconnect the CO2 and power supplies. See
section 5 pages 8-9 for lifting, handling, and transport procedures.

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SECTION

Troubleshooting

5
12

Temperature
Temperature too high
1. Controller set too high - see section 7, Setting the Temperature
2. Controller failed on call Customer Service.

Temperature too low

1. Over temperature too low see section 7, Setting the Over Temperature Safety
2. Controller set too low - see section 7, Setting the Temperature
3. Unit has not recovered from power failure or being turned off - incubator needs to warm up and
stabilize for 8 hours
4. Unit has not recovered from door opening - wait for the display to stop changing
5. Element Failure - see if the heating light is on and compare current draw to the data plate information
6. Controller failure - confirm with front panel lights that controller is calling for heat
7. Thermostat failure - confirm with front panel lights that Over Temperature is operating correctly

Unit will not heat over a temperature that is below set point
1. Confirm that the set point is set high enough - turn the Over Temperature Safety Control
counterclockwise and see if HEATING or OVERTEMP lights are illuminated
2. Check the calibration - this can be done by using an independent, certified reference thermometer and
by following the instructions in section 7, Calibrating The Temperature

Unit does not heat up at all

1. Verify that controller is asking for heat by looking for HEATING light display - if pilot light is not
on during the entire initial start up, there is a problem with the controller
2. Check amperage
3. Do all of the controller functions work? - if not contact Customer Service
4. Is the Over Temperature Thermostat set high enough? - For diagnostics, the Over Temperature Safety
Control should be turned clockwise until it stops with the pilot light never on
5. Check to see if the fuse or circuit has blown

Chamber temperature unstable

1. +/- 0.1 is normal variance
2. Is the ambient room temperature radically changing? This could be a result of a number of things:
Door opening too often, room airflow from heaters or air conditioning - stabilize ambient conditions
3. Calibration sensitivity - contact Customer Service
4. Over Temperature Thermostat is set too low - check if pilot light is on continuously. Turn controller knob
completely clockwise to see if problem is solved, then follow instructions in section 7 for setting
Over Temperature

Unit will not maintain set point

1. Assure that set point is at least 8 degrees over ambient room temperature
2. See if ambient temperature is fluctuating. If so stabilize

Cannot adjust set points or calibration

1. Reset entire unit by turning off and then on. If happens repeatedly, contact Customer Service

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SECTION

Troubleshooting Continued

5
12

CO2 Level
Overshoots set point but stabilizes - display and fyrite match
1.
2.
3.
4.

Tank pressure is too high, see section 8

CO2 sensor is partially plugged with dirt or condensation - contact Customer Service
Regulator is set wrong, see section 8
Pump filter partially plugged - contact Customer Service

Overshoots set point and continues to rise - display and fyrite match
1.
2.
3.
4.
5.

Debris in solenoid causing it to leak continuously - contact Customer Service

Solenoid failed while open - contact Customer Service
Controller output failed or shorted - contact Customer Service
CO2 sensor or interface failure - contact Customer Service
CO2 sensor plugged by debris or condensation - contact Customer Service

Rises very slowly

1.
2.
3.
4.
5.
6.

Filter is too dirty or partially plugged - replace filter

Hose is kinked or leaking - repair or replace hoses
CO2 tank regulator set too low - see section 8
Poor or faulty door seal - call customer service
Restrictor partially plugged - clean restrictor
CO2 tank regulator turned off - turn on regulator

Never Rises
1.
2.
3.
4.
5.
6.
7.

CO2 filter plugged - replace CO2 filters

CO2 hose blocked - repair or replace hose
CO2 tank empty - change tank
CO2 controller ouput failed while open - contact Customer Service
Solenoid failed while closed - contact Customer Service
Set point is at 0.0 and has not been reset - see section 7
CO2 tank regulator is not on - turn on regulator

If unstable - display or actual reading varies around set point

1.
2.
3.
4.
5.
6.

Door not sealing properly - check door seal

Defective fyrite pump or tubing - Validate Fyrite calibration
Defective tubing - check tubing and replace if necessary
Top of unit is exposed to cold air drafts - check the units position and move the unit to appropriate spot
Unit being operated without shadow box cover
Incubator is too heavily loaded

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SECTION

Troubleshooting Continued

5
12

CO2 Level Continued

Display and Fyrite reading do not match

1. Calibration error - see section 7

2. CO2 sensor, interface, or controller failure - contact Customer Service

Set points or calibration is locked up and can not be adjusted

1. Turn unit off and on to reset processor in controller
2. If it happens repeatedly - contact Customer Service

Will not hold calibration - display stable but Fyrite reading varies
1.
2.
3.
4.
5.
6.

Top of unit exposed to cold air drafts

Unit is being operated without shadow box cover in place
Condensation collecting on CO2 sensor
CO2 sensor or interface failure
Unit is incorrectly calibrated - see section 7
Taking fyrite reading too soon after the door has been opened

Mechanical / Other
Door not sealing
1. Check physical condition of gasket
2. Confirm that door latch pulls door tightly
3. Assure gasket is in original location

Controller is on at all times and locked up

1. Turn unit off and on to reset. If condition on front panel can not be changed, call Customer Service

Front panel displays are all off

1. Make sure unit is plugged in, check fuse, power switch, and power supply

Unit or wall fuse/circuit breaker is blown

1. Check wall power source and see what other loads are on wall circuit

Unit will not turn on

1. Check power source - check fuse and circuit breaker on unit or in wall and check power switch

Contamination in chamber
1. See cleaning procedure in Section 11. Develop and follow standard operating procedure for
specific application: include cleaning technique and maintenance schedule

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SECTION

SHEL LAB CO2 Incubator

Replacement Part Numbers

11
13

Description (Listed Alphabetically)

CO2 Display Board
CO2 Filter
Door Heater (Internal)
Door Switch (Stops CO2 from injecting when door is open)
Element Assembly
Air Jacket Circulating Fan Blade
Fan Motor
Gasket, 8 Feet (seals against viewing door)
Glass Door Assembly
Humidity Pan

Part Number for 115V

1750819
100199
2350529
X1000022
9570926
2600502
4880527
3450644
9521195
7930514

Part Number for 220V

1750819
100199
2350528
X1000022
9570927
2600502
4880528
3450644
9521195
7930514

Digital IR CO2 Sensor

Leveling Feet
Main Control Board
Over Temperature Protection Thermostat
Air Jacket Circulating Motor
Outer Door Gasket
Power cord inlet fuse 12.5 amp 250v / 15amp 110v
Pilot Light, Red
Power Cord Set
Power Switch
Recorder Jack
Shelf
Solenoid Valve 12vdc
Access Port Stopper
Temperature Display Board
Decontamination Temperature Limit Thermostat
Temperature Probes
Motor Cooling Fan Blade
Decon Cycle Display Board
Usb Interconnect Board
12vdc Muffin Fan
Sampling Pump
12vdc Power Supply

8320511
2700506
1750821
10000J
4880527
3450534
3300520
200020
1800510
7850553
100163
5121109
8600560
7750506
1750818
1750759
103070
104073
1750820
1750817
1850515
6700558
6750502

8320511
2700506
1750821
10000J
4880528
3450534
3300516
200020
1800500
7850553
100163
5121109
8600560
7750506
1750818
1750759
103070
104073
1750820
1750817
1850515
6700559
6750502

Page 26

SECTION

SHEL LAB CO2 Incubator

Unit Specifications
Return to Table of Contents

Model Number
3552 & 3552-2

Interior Dimensions Exterior Dimensions

W x D x H (Inches)
W x D x H (Inches)
20.5 x 19.75 x 25.25 28.25 x 31x 39.25

Model Number
3552 & 3552-2

Shipping Weight
275 lbs (127kg)

Model Number
3552 & 3552-2

Temperature Range
Ambient +8C to 50C

14

Chamber Capacity
164 cubic liters

Net Weight
237 lbs (100 kg)

Control
+/- 0.1C

Temperature Uniformity CO2 Range

+/-0.35C @ 37C
0 - 20%

Decontamination Temperature is 180C for 120 minutes.

Model Number
3552
3552-2

Amperage
12.0 Amp.
6.0 Amp.

Voltage
120 VAC
230 VAC

Hertz
60 Hz
50/60 Hz

RECOMENDED INCUBATOR ACCESSORIES:

1. CO2 Tank Switch
2. Fyrite CO2 Gas Analyzer
3. In-Line Hepa Filter
4. Caster Platform (sku# 9000574)
5. Stacking Platform (sku# 9000575)

Page 27

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Page 28

SECTION

Wiring Diagram for Models

3552 & 3552-2

15