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    Pectin Media

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    suresh_kumar16174475
    This document describes MP-5 Medium, which is used to detect pectinolytic microorganisms that produce polygalacturonase. It lists the ingredients in the medium and provides instructions for preparation and use. Two example organisms, Aspergillus brasiliensis and Fusarium moniliforme, are identified as positive for polygalacturonase production when tested with this medium.

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    Pectin Media

    Uploaded by

    suresh_kumar16174475
    479 views2 pages
    This document describes MP-5 Medium, which is used to detect pectinolytic microorganisms that produce polygalacturonase. It lists the ingredients in the medium and provides instructions for preparation and use. Two example organisms, Aspergillus brasiliensis and Fusarium moniliforme, are identified as positive for polygalacturonase production when tested with this medium.

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    This document describes MP-5 Medium, which is used to detect pectinolytic microorganisms that produce polygalacturonase. It lists the ingredients in the medium and provides instructions for preparation and use. Two example organisms, Aspergillus brasiliensis and Fusarium moniliforme, are identified as positive for polygalacturonase production when tested with this medium.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    479 views2 pages

    Pectin Media

    Uploaded by

    suresh_kumar16174475
    This document describes MP-5 Medium, which is used to detect pectinolytic microorganisms that produce polygalacturonase. It lists the ingredients in the medium and provides instructions for preparation and use. Two example organisms, Aspergillus brasiliensis and Fusarium moniliforme, are identified as positive for polygalacturonase production when tested with this medium.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    M596

    MP-5 Medium

    MP-5 Medium is used for the detection of pectinolytic microorganisms especially those producing polygalacturonase.

    Composition**
    Ingredients
    Pectin
    Monopotassium phosphate
    Disodium phosphate
    Ammonium sulphate
    Yeast extract
    Ferrous sulphate
    Magnesium sulphate
    Calcium chloride
    Boric acid
    Manganese sulphate
    Zinc sulphate
    Copper sulphate
    Molybdenum trioxide
    Agar
    Final pH ( at 25C)
    **Formula adjusted, standardized to suit performance parameters

    Gms / Litre
    5.000
    4.000
    6.000
    2.000
    1.000
    0.001
    0.200
    0.001
    0.00001
    0.00001
    0.00007
    0.00005
    0.00001
    15.000
    5.50.5

    Directions
    Suspend 33.2 grams in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving
    at 15 lbs pressure (121oC) for 15 minutes. Mix well and pour into sterile Petri plates.
    Note : Due to presence of various inorganic salts, slight precipitate may develop upon heating. Shake well before pouring into
    sterile Petri plates.
    Polysaccharride precipitant solution : Dissolve 1.0 gm of hexadecyltrimethyl ammonium bromide in 100 ml of water and the
    solution is sterilized by autoclaving if desired.

    Principle And Interpretation


    Pectin is an important cell wall component of higher plants that helps in cementing plant cells together. Most pectin-degrading
    organisms are associated with raw agricultural products and with soil. Detection of pectinolytic activity of an organism is
    carried out either by observing depression in the gel around the colony where the substrate has been degraded or by flooding
    the plate with a precipitant solution.
    MP-5 Medium is used for the detection of pectinolytic organisms especially those producing polygalactouronase. MP-5 medium
    is recommended by APHA for detecting pectinolytic organisms (1).
    Detection of polygalactouronase by plate assay is generally done by lowering the pH of the medium, designed for detection of
    pectate lyase, (i.e. MP-7 Medium) to 6 or below so that polygalactouronase will be active and pectate lyases will be inactive (2,
    3). Thus the acidic pH of MP-5 Medium is the main parameter used to distinguish polygalactouronase producers from pectate
    lyase producers. A 1.0% aqueous solution of hexadecyltrimethyl ammonium bromide (4) is used to detect pectinolytic activity.
    After incubating the plates for 2-3 days at 30-35C, the polysaccharride precipitant is poured over the surface of the plate taking
    care not to dislodge the colonies. Zones of pectin hydrolysis will be visible usually within few minutes and can be best viewed
    against dark background. The reagent precipitates the intact pectin in the medium whereas pectinolytic growth is surrounded
    by a clear halo in an opaque medium. High phosphate level in the medium is required to observe pectinolytic activity.

    Quality Control
    Please refer disclaimer Overleaf.

    HiMedia Laboratories

    Technical Data

    Appearance
    Cream to yellow homogeneous free flowing powder
    Gelling
    Firm, comparable with 1.5% Agar gel.
    Colour and Clarity of prepared medium
    Yellow coloured clear to slightly opalescent gel forms in Petri plates
    Reaction
    Reaction of 3.32% w/v aqueous solution at 25C. pH : 5.50.5
    pH
    5.00-6.00
    Cultural Response
    Cultural characteristics observed after an incubation at 35-37C for 2-3 days.
    Cultural Response
    Organism
    Cultural Response
    *Aspergillus brasiliensis
    ATCC 16404

    Fusarium moniliforme

    Polygalacturonase
    production
    positive, clear
    halo around
    the colony
    when flooded
    with 1%
    polysaccharride
    precipitant.
    positive, clear
    halo around
    the colony
    when flooded
    with 1%
    polysaccharride
    precipitant

    Storage and Shelf Life


    Store below 30C in tightly closed container and the prepared medium at 2-8C. Use before expiry date on the label.

    Reference
    1. Downes F. P. and Ito K. (Eds.), 2001, Compendium for the Microbiological Examination of Foods, 4th Ed. APHA,
    Washington, D.C.
    2. Hankin L. and Anagnostakis S. L., 1975, Mycologia 67:597.
    3. Vaughn R. H., Balatsouras G. D., York G. K. II and Nagel C. W., 1957, Food Res. 22:597.
    4. Jayasankar N. P. and Graham P. H., 1970, Can J. Microbiol., 16:1023.
    Revision : 1 / 2011

    Disclaimer :
    User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
    this and other related HiMedia publications. The information contained in this publication is based on our research and development
    work and is to the best of our knowledge true and accurate. HiMedia Laboratories Pvt Ltd reserves the right to make changes to
    specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
    for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
    be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.
    HiMedia Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147
    1919 Email: [email protected] Website: www.himedialabs.com

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