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    E-Cigs SF Posterboard 2

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    E-Cigs SF Posterboard 2

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    e-cigs sf posterboard 2

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    E-Cigs SF Posterboard 2

    Uploaded by

    api-240966173

    Copyright:

    © All Rights Reserved
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    of 1

    DETECTION AND QUANTIFICATION OF NICOTINE IN ELECTRONIC CIGARETTES BY HPLC

    Electronic cigarettes are a new and widely popular, but fairly


    untested and unregulated technology. This project aims to
    put electronic cigarette liquid manufacturers to the test by
    determining the amount of nicotine in their products. Using
    reverse-phased high performance liquid chromatography,
    standard solutions of nicotine at concentrations of 50 ppm,
    100 ppm, 200 ppm, 400 ppm, and 600 ppm were run in order
    to determine a standard calibration curve. By comparing
    these standards to five different electronic cigarette liquids,
    nicotine was detected and quantified.
    ABSTRACT
    INTRODUCTION
    Materials
    Preparation of Nicotine Standards
    Standards were prepared using a serial dilution method, with 50:50 MeOH H2O acting as
    the solvent. Astock solution of 600 ppm nicotine was made from a Sigma-Aldrich 1mg/
    ml nicotine solution. From this stock, samples of 400 ppm, 200 ppm, 100 ppm, and 50
    ppm were created. Solutions were made in eppendorf tubes and filtered through 0.45m
    filters using syringes upon transfer to HPLC vials.
    Preparation of Electronic Cigarette Liquids:
    Electronic cigarette liquids of two different flavors and brands were purchased. From
    Savor Vapor, menthol refill liquid bottles of 0mg, 6mg, and 12mg were purchased, and
    from iFill Vape, Bahama Mama liquids of 0mg and 6mg were purchased. Approximately
    1ml of each concentration and flavor was filtered through a 0.45m syringe filter and
    into HPLC vials.
    Preparation of Mobile Phase:
    An isocratic method was used, initially with a 25:75 MeOH H2O mobile phase (with
    0.05% formic acid to adjust pH). Later runs, however, were done using a 20:80 MeOH
    H2O mobile phase which was filtered prior to running.
    MATERIALS AND METHODS
    Given these results, we can conclude that there is, in fact, more nicotine per bottle of refill
    liquid than these companies are claiming. Every liquid tested showed more nicotine than
    advertised, including those labeled as nicotine free. We can therefore further conclude that
    our hypothesis was correct.
    DATA
    CONCLUSION
    REFERENCES
    Setting for HPLC Reverse-phase
    Column Zorbax Eclipse XDB-C8 (4.6 x 150mm) 5-micron
    Mobile Phase 20:80 MeOH H2O (with 0.05% formic acid to adjust pH)
    Injection Volume 5 l
    Retention time 2 minutes
    Wavelength 254 nm
    Flow Rate 0.7 ml/min
    OBJECTIVE/HYPOTHESIS
    Objective:
    To determine amount of nicotine in electronic cigarette refill
    liquids.
    Hypothesis:
    We hypothesize that the electronic cigarette refill liquids are
    improperly labeled and contain more nicotine than advertised.
    ACKNOWLEDGEMENTS
    RESULTS/DISCUSSION
    Wed like to thank Dr. Malhotra for her constant counsel and support, Dr. Tannaci for
    providing us with the chemicals we needed, Karen Kearsley of Agilent Technologies for
    generously donating a column, and Dr. Cauchon for his HPLC expertise. Without the
    guidance of these great mentors and professionals, our project would not have been the
    great learning experience it was.
    y = 9.5448x - 9.7338
    R! = 0.9981
    -1000
    0
    1000
    2000
    3000
    4000
    5000
    6000
    7000
    0 100 200 300 400 500 600 700
    P
    e
    a
    k
    A
    r
    e
    a

    Concentration (ppm)
    Nicotine Standard Calibration Curve
    Concentration
    (ppm)
    Nicotine (g) per
    5 l Injection
    Nicotine (mg) per
    15 ml Bottle
    Menthol 0mg 44.54 0.2227 0.681
    Menthol 6mg 1330.172 6.651 19.953
    Menthol 12mg 3987.691 19.938 59.814
    BM 0mg 90.96 .453 1.359
    BM 6mg 1469.906 7.35 22.05
    0
    10
    20
    30
    40
    50
    60
    70
    Bahama
    Mama
    0mg
    Bahama
    Mama
    6mg
    Menthol
    12mg
    Menthol
    6mg
    Menthol
    0mg
    N
    ic
    o
    tin
    e
    (m
    g
    ) p
    e
    r
    B
    o
    ttle

    Electronic Cigarette
    Comparison of Nicotine Levels
    Labeled Amount
    Amount We Detected
    y = 0.005x
    0
    0.5
    1
    1.5
    2
    2.5
    3
    3.5
    0 100 200 300 400 500 600 700
    M
    a
    s
    s
    (u
    g
    ) p
    e
    r
    I
    n
    je
    c
    tio
    n

    Concentration (ppm)
    Nicotine Standard Injection Curve
    FURTHER WORK

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