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Pathogen Testing

1-2 TestThe First Widely Accepted Single-Use Salmonella Test


The 1-2 Test is a patented, single-use test for Salmonella used by analysts
worldwide. It combines proprietary science with practical application, resulting
in proven performance and ease of use. Each kit is self-contained and includes
all reagents necessary to run the test. Test results are read visually and are easy
to interpret without the use of equipment.

Accurate, Reliable and Validated Results


Extensive multi-laboratory and field validation studies have shown that the
1-2 Test is highly accurate and specific. 1-6 The proprietary 1-2 Test antibody
captures a wide range of Salmonella, without reacting with most common
cross-reactors. The 1-2 Test is suitable for testing all food products, food
ingredients and environmental samples.

Simple to use.
Advanced Science.
Rapid, Reliable Results
Results are available as soon as 14 hours after enrichment.
Simple to Use
The 1-2 Test requires only two to three minutes of hands-on time to run. Each simple step
of the preparation sequence can be performed on an individual unit or simultaneously on
multiple units.
Flexible
The single-use format of the 1-2 Test provides the flexibility to run any number of tests
depending on your testing needs.
Actual Size
Visual Results
Results are interpreted visually by observing the development of an Immunoband, a
characteristic immobilization pattern of cells. No equipment is required to read results.
Extensively Validated
AOAC Official Method 989.13 and other international validations.
1-2 Test Kit Contents
Each test kit is self-contained and comes with disposable 1-2 Test units, activation reagent and
proprietary antibody preparation solution. A 1-2 Test with an empty sideport (ESP) is also
available.

Simple to Use. Advanced Science.

The 1-2 Test is highly sensitive and specific. It uses a unique combination of a built-in selective enrichment, the
immunodiffusion principle, and a proprietary preparation of antibodies to detect Salmonella.

Diffusion
of 1-2 Test
Inoculation
antibody Immunoband
Chamber
Motility
chamber

Movement of
Salmonella

Analyst adds enriched sample Salmonella, if present, begins As the Salmonella encounters
to the inoculation chamber to move through the selective the diffusing antibodies they are
and a drop of the 1-2 Test medium into the motility immobilized. A characteristic
antibody to the void in the chamber. At the same time, three-dimensional Immunoband
peptone-based gel in the the 1-2 Test antibody starts will be formed if the test is
motility chamber. The 1-2 Test diffusing into the motility positive. Results are read visually.
is incubated. chamber in a radial pattern.

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References
1. J. AOAC Int. 72, 303(1989)
2. J. Food Prot. 53, 656(1990)
3. J. Food Microbiology. 11, 253(1994)
4. J. AOAC Int. 78, 59(1995)
5. J. AOAC Int. 78, 987(1995)
6. J. AOAC Int. 78, 993(1995)

Worldwide Headquarters Bellevue, WA USA tel 425.603.1123 fax 425.603.0070 www.biocontrolsys.com


European Office Montesson, France tél +33 (0)1 30 15 78 08 fax +33 (0)1 30 15 78 09 [email protected]
Latin America Office Campinas, Brasil tel/fax +55 19 3231 2502 +55 19 3233 7988 [email protected]
United Kingdom Office London, UK tel +44 (0)1 449 721420 fax +44 (0)1 449 723120 [email protected]

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