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    08 Su Hinh Thanh Mot Dap Ung Mien Dich

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    Van Kim Thanh
    1. The document discusses the formation of an immune response, comparing the primary and secondary responses. The primary response is slower, with IgM production, while the secondary response is faster and produces IgG. 2. It describes the pathways of antigen processing and presentation via MHC class I and II, where intracellular pathogens activate CD8 T cells and extracellular pathogens activate CD4 T cells. 3. Cellular events in the primary and secondary antibody responses are compared, showing faster responses, class switching, and memory cell formation in secondary responses.

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    08 Su Hinh Thanh Mot Dap Ung Mien Dich

    Uploaded by

    Van Kim Thanh
    28 views20 pages
    1. The document discusses the formation of an immune response, comparing the primary and secondary responses. The primary response is slower, with IgM production, while the secondary response is faster and produces IgG. 2. It describes the pathways of antigen processing and presentation via MHC class I and II, where intracellular pathogens activate CD8 T cells and extracellular pathogens activate CD4 T cells. 3. Cellular events in the primary and secondary antibody responses are compared, showing faster responses, class switching, and memory cell formation in secondary responses.

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    1. The document discusses the formation of an immune response, comparing the primary and secondary responses. The primary response is slower, with IgM production, while the secondary response is faster and produces IgG. 2. It describes the pathways of antigen processing and presentation via MHC class I and II, where intracellular pathogens activate CD8 T cells and extracellular pathogens activate CD4 T cells. 3. Cellular events in the primary and secondary antibody responses are compared, showing faster responses, class switching, and memory cell formation in secondary responses.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    28 views20 pages

    08 Su Hinh Thanh Mot Dap Ung Mien Dich

    Uploaded by

    Van Kim Thanh
    1. The document discusses the formation of an immune response, comparing the primary and secondary responses. The primary response is slower, with IgM production, while the secondary response is faster and produces IgG. 2. It describes the pathways of antigen processing and presentation via MHC class I and II, where intracellular pathogens activate CD8 T cells and extracellular pathogens activate CD4 T cells. 3. Cellular events in the primary and secondary antibody responses are compared, showing faster responses, class switching, and memory cell formation in secondary responses.

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    © All Rights Reserved
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    1

    S HNH THANH MOT


    AP NG MIEN DCH
    GS Pham Hoang Phiet
    BS Huynh Thanh Bnh
    2
    .
    .
    ong hoc mot ap ng mien dch
    3
    So sanh ap ng th 1 va th 2
    MD
    Tnh chat
    Th 1 Th 2
    Dien
    tien
    Tiem an 1-2 tuan 3-5 ngay
    Tang SLKT Tang nhanh Tang rat nhanh
    Bnh on 2-3 tuan Nhieu thang- nam
    Giam sut Nhanh T t
    So
    lng
    Cao hn th 1 nhieu
    Chat
    lng
    Lp KT IgM IgG
    Ai lc vi
    KN
    + +++
    4
    Te bao trnh dien
    khang nguyen
    HLA lp II TCD4
    (peptid 15-34 aa)
    HLA lp I TCD8
    (peptid 9 aa)
    5
    Class I MHC Pathway
    Viral protein is made
    on cytoplasmic
    ribosomes
    Plasma membrane
    Proteasome
    degrades
    protein to
    peptides
    Peptide transporter
    protein moves
    peptide into ER
    MHC class I alpha
    and beta proteins
    are made on the rER
    Peptide associates
    with MHC-I complex
    Peptide with MHC
    goes to Golgi body
    Peptide passes
    with MHC from Golgi
    body to surface
    Peptide is presented
    by MHC-I to CD8
    cytotoxic T cell
    Golgi body
    rER
    Globular viral
    protein - intact
    6
    Class II MHC Pathway
    Globular
    protein
    Endocytosis
    Protein is processed to
    peptides in endosome
    or lysosome
    Endosome
    Lysosome
    Fusion of endosome
    and exocytic vesicle
    Endoplasmic reticulum
    Class II MHC
    Synthesis
    3 chains: , and Ii
    Golgi
    body
    Exocytic vesicle fuses
    with endosome
    releasing Ii from dimer

    Ii
    Immunodominant
    peptide binds
    to class II MHC
    Endosome fuses with
    plasma membrane
    Peptide MHC-II
    complex is presented
    to CD4 helper T cell
    CD4 helper T cell
    7
    IL2 (IL4)
    TB,Tc, (B)
    Hoat hoa Lympho T
    1
    2
    3
    4
    8
    Points Concerning Antigen
    Processing and Presentation
    1. Location of pathogen
    viruses in cytosol, MHC class I
    pathway, Tc response
    extracellular bacteria, MHC class II
    pathway, Th2 response, Ab formation
    intracellular bacteria, MHC class II
    pathway, Th1 response
    9
    Points Concerning Antigen
    Processing and Presentation
    2. Peptides derived from both self and
    non-self proteins can associate with
    MHC class I and class II molecules.
    3. Chemical nature of MHC groove
    determines which peptides it will
    bind.
    10
    Hoat hoa Lympho bao
    T B
    Tn hieu
    KN
    (1)Ti (TCR) KN
    (2) CD4 (T
    H
    )
    HLA
    II
    (APC)
    sIg (BCR) KN
    Tn hieu
    th 2
    (1) IFN- (T
    H
    )
    (2) IL1 (APC)
    IL2,IL4, IL5
    11
    Hoat hoa
    Lympho B
    12
    S hp tac gia
    ai thc bao,
    lympho T va
    lympho B
    13
    Cellular Events in 1
    o
    Response to
    T-dependent Ags
    Lag
    Clonal selection
    Log
    IgM
    Class switching
    Stationary
    Decline
    Memory Cell Pool
    IgM
    Memory Cells
    IgG
    1
    o
    Ag
    14
    Cellular Events in 2
    o
    Response to
    T-dependent Ags
    Lag phase
    Virgin cells
    Memory cells
    Log phase
    Pool size
    IgG, IgA or IgE
    Stationary
    Decline
    Sustained
    production
    IgM
    Memory
    Cells
    IgG
    IgG
    Memory
    Cells
    Memory
    Pool
    Virgin B cell
    15
    Hieu ng Hapten-tai
    Hapten khong co tnh gay MD
    Hapten phai gan vi 1 protein tai =>
    phc hapten tai (hapten-carrier
    complex)
    Th nhan dien tai, B nhan dien hapten
    Tai th 1 va th 2 phai giong nhau.
    16
    S hp tac han che trong nhom phu hp mo
    (hien tng Zinkernagel va Doherty)
    Mouse
    Strain A or B
    Immunize
    with antigen
    Period of 7 days
    T cells isolated
    from lymph nodes
    T cell primed with
    antigen
    Mouse
    Strain A or B
    Peritoneal macrophage
    Antigen
    Macrophage presents antigen
    Macrophages and
    T cells are co-cultivated
    Anti X-induced
    Macrophage T-cell proliferation
    Strain A A yes
    Strain A B no
    Strain B B yes
    Strain B A no
    Assay DNA
    synthesis
    after 48 hr
    17
    Tc Cell Self MHC Restriction
    Mouse
    Strain A or B
    Immunize
    with virus
    Period of 7 days
    Virus-specific
    cytotoxic T cells (CTLs)
    isolated from spleen
    Virus-specific
    CTLs
    Mouse
    Strain A or B
    Purify
    Fibroblasts
    CTLs and fibroblasts
    are co-cultivated
    Spleen Fibroblast 51Cr release
    CTLs (target cells) from fibroblasts
    immunized infected (lysis)
    with with
    Strain A A yes
    Strain A B no
    Strain B B yes
    Strain B A no
    Assay 51Cr
    release
    Isolate spleen
    Infect fibroblasts
    with virus and
    radiolabel with 51Cr
    51Cr labeled fibroblast
    presents antigen
    18
    Self MHC Restriction in the Thymus
    4 -
    8 low
    4 low
    8 low
    4 + 8 +
    TCR
    4 + 8 +
    TCR
    macrophage
    Productive TCR
    rearrangement
    Non-productive TCR
    rearrangement
    Recognise self MHC
    Not recognise self MHC
    TCR does not
    recognise self antigens
    Negative selection
    TCR recognises
    self antigens
    APOPTOSIS
    4 + 8 +
    TCR
    4 - 8 -
    Sub-capsular region
    Cortex
    Cortico-medullary region
    4 + 8 -
    TCR
    4 - 8 +
    TCR
    vessel
    Medulla
    19
    KN khong le thuoc thymus
    Cau truc: ai phan t do trung phan cac n
    v nho (lipopolysaccharide, dextran)
    Cac epitop giong nhau tren ai phan t lien
    ket vi nhieu sIg tao thanh am (hien tng
    oi mu) => hoat hoa Lympho B thanh tng
    bao => SX KT
    KT thuoc lp IgM, khong tao ra tr nh MD
    20
    Hien tng oi mu

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