C196-E061G

Prominence

Shimadzu High Per for mance Liquid Chromatograph

Do you know Prominence ?

Prominence
Shimadzu High Perfor mance Liquid Chromatograph A Networ k-Compatible HPLC Designed for a Rapidly Advancing Infor mation Society
With fierce competition in many industries, stronger legal restrictions, and the rapid spread of IT, business is changing daily at a blinding pace. In this climate, the problems and needs of customers are diversifying. This applies to high-performance liquid chromatographs, which are used for a variety of purposes, such as R&D, quality control, and environmental measurement. In order to adapt quickly to these diversifying user needs, Shimadzu Corporation has developed the "Prominence" high-performance liquid chromatograph. In addition to bolstering basic performance, this product can be used as part of a comprehensive control system that can interface with advanced networks. It enables the centralized management of devices and data, thereby ensuring efficient analysis. Advanced Networ ks with Web Browser Control Capability
Centralized Management of Analytical Instruments

Optimization as Front-End Component for MS

Outstanding Performance

Full Automation of Analysis Wor kflow

Freedom from the Laboratory

Contents

P 04 - Features of Series

P 16 - Auto-Sampler

P 24 - Prominence Compatible Units

P 08 - System Variations

P 18 - Column Oven

P 34 - Optional Accessories

P 10 - List of Units

P 20 - Absorbance Detector

P 38 - MS Detector

P 12 - System Controller

P 22 - Specifications

P 14 - Solvent Delivery Unit

P 24 - LCsolution

Netw or k

Advanced Networks with Web Browser

[ Centralized Management of Analytical Instruments ]

Ensuring the efficiency of analysis work and the security of data by centralizing the management of analytical instruments and data will become an integral par t of laborator y practice in the future. The Prominence system achieves these crucial requirements now by

C o n n e c t i o n to L C Wo rk s tations i n t h e N e two rk
The Prominence system can connect to LC workstations in the network via Ethernet. In addition to increasing the degree of freedom of the installation and fostering more efficient use of laboratory space, it enables access to the HPLC system from multiple client PCs and provides a flexible analytical environment.

Lab

Office

Ethernet LAN

Example of network environment using Prominence

To t a l S u pp o r t C ov e rin g A n alysis, D a t a M a nag eme n t, an d D ev ice M a n ag e m e n t

Analysis and data processing are performed with LCsolution or CLASS-VP (on an LC workstation), data management is performed with CLASS-Agent, and the remote monitoring of devices, maintenance management, and scheduling are performed from a Web browser, thereby providing total support.

Device Management from a Web Browser (IE)

Data Analysis with LCsolution or CLASS-VP

Data Management with CLASS-Agent

Control Capability
providing efficient and comprehensive suppor t for data management and instrument control via the network, where users can monitor analysis, maintenance management, and analysis scheduling in workgroup units.

S y s t e m Co n tro l U s in g a We b B row se r
Use any computer with Microsoft Internet Explorer 6.0 to control and monitor the Prominence HPLC system. Connection to the CBM-20A with a static IP address requires no special software to control and monitor HPLC systems on the network from the client PC. Easily check the status of networked HPLC systems, or monitor consumable parts for increased work efficiency.
Up to 10 LC units managed as one workgroup. One of the CBM-20A's Web-server functions is a memo function. This function can be used for scheduling the use of devices by sending memos such as "Use next week" or "Perform maintenance".

Column information is managed automatically via the CMD (Column Management Device).

Each device can be controlled and its operating status can be monitored.

The maintenance status of devices can be checked at a glance.

Innovative Network Compatibility

M u l t i - v e nd o r C o mp atib ility t h ro u g h We b B row se r
Operation is performed from a PC using a Web browser; therefore, even users utilizing another vendor's data processing system can perform monitoring, control, and maintenance management for the Prominence system with ease. It is also possible to use Prominence as the front-end component in an LC-MS/LC-MSMS system while controlling it from an MS workstation.

Web Browser

Other vendor's Data Processing Software

Other vendor's LC-MS/LC-MSMS System

Per for m ance

Optimization as a Front-End Component for MS

[ Outstanding Performance ]

At a time when the impor tance and widespread use of LC-MS is increasing, an HPLC system must be able to attain a high level of performance as an MS front-end component. In addition to a high level of basic performance, including micro-range solvent deliver y, high throughput, and the suppression of carr yover, the Prominence system is a truly effective HPLC system that enables high-level LC-MS analysis. Su p e ri o r M i c ro -R an g e S o lve n t Deliver y Performance
In addition to enhancing the micro-volume plunger, which already has an established reputation, by refining the control firmware, the accuracy of the flow rate and gradient concentration at lower flow rates have been significantly improved.
P14 Solvent Delivery Unit

Ul tr a - h i g h T h ro u g h p u t
Our fastest sample injection speed (10 sec. for an injection of 10 L) ensures greater MS throughput. Also, mounting a rack changer makes it possible to perform continuous processing for up to 12 microplates.
P16 Auto-Sampler

Sa m p l e C a rr yove r R ed u c e d to an Absolut e M inimum

Special coating needle

A special coating on the needle and a low-adsorption valve ensure hardly any carryover, even with basic or hydrophobic compounds. Also, using the optional multi-rinse mode ensures that the optimum rinsing method is selected, even for sample constituents that are difficult to rinse, such as proteins.

Contact area minimized

P16 Auto-Sampler

Sy st e m Ex p a nsio n s to S u it a Va riet y of Purposes

This system offers superior expandability and can be configured into a variety of compact systems for MS, such as dual high-pressure gradient systems and 2D HPLC systems.

P8 System Variations
6

Aut om ati on

Full Automation of Analysis Work

[ Freedom from the Laboratory ]

The basic requirements of an HPLC system remain constant: it must be easy for anyone to use and it must yield highly reliable data. With the Prominence system, all the units operate in harmony, achieving a level of automation as yet unseen with modular systems, and significantly reducing the effor t and cost required to perform analysis.

Au to -S tar t up and Aut o- Shut down

Combining the Harmonize functions for each unit makes it possible to fully automate all the processes involved in analysis workflow, from startup to shutdown. Simply set the mobile phase and sample and start the system. The system will recognize the operating status and execute analysis. This significantly reduces the time the user must devote to analysis at startup. Main Types of Automated Analysis Procedures
Starting and stopping of system operation at specified times Replacement of mobile phase (purge) Rinsing and cooling of column Automatic judgment of baseline stabilization Sample injection

E x p er t Funct ion
The system is equipped with a function that continuously monitors the operating status and, if there is a problem, provides notification and tries to resolve it. For example, if the pressure falls below a certain value during serial analysis, the function detects the problem, purges the mobile phase, and then resumes analysis after restoring normal status. The reliability of operating performance is also increased with a variety of diagnostic functions. Main Types of Automated Check Items
Remaining amount of mobile phase Noise/drift check Vial detection

Au to -Validat ion
The Prominence system also incorporates consideration of validation. IQ/OQ at the time of installation, operational qualification for each unit in periodic inspections, and management of maintenance information can all be executed with ease. This means that inspections and modifications can be performed quickly and simply, significantly reducing the validation cost. Also, using an optional CMD (Column Management Device) makes it possible to recognize the column name and serial number, and to leave a record of information, such as the amount of mobile phase that has pumped and the number of sample injections. Automated Operational-Qualification (OQ) Items
Accuracy of solvent-delivery flow rate Solvent-delivery pulsation Lamp energy Wavelength accuracy (built-in, low-pressure, mercury lamp)

System V ar i a t i o n s

Flexible System Variations

By combining the modules appropriately, the Prominence system can be configured in a variety of ways to suit different analytical objectives and methods. Hi g h - Pre s s u re Gra d ien t S y s tem [ Prominence HGE-UV]
Configuration: High-performance system that uses the 2-solvent high-pressure mixing gradient elution method. Applications: In addition to general analysis, it can be used as an LC-MS front-end component for micro and semi-micro HPLC. Incorporating an SPD-M20A high-sensitivity photodiode array detector makes it easy to examine specificity-related items, such as identifying impurities and checking peak purity.
P/N
228-45011-38 228-45002-XX 228-45018-32 228-45093-93 228-45007-XX 228-45010-XX 228-45004-XX

Description
System Controller Solvent Delivery Unit Online Degasser Mixer 0.5-2.6mL HP Auto-Sampler Column Oven UV-VIS Detector LC Workstation

Model Name
CBM-20Alite LC-20AB DGU-20A3 SIL-20AC CTO-20AC SPD-20AV LCsolution Single

Q'ty
1 1 1 1 1 1 1 1

Low - Pre s s u re Gra d ien t S y s tem [ Prominence LGE-UV]

Configuration: Flexible system that uses the 4-solvent low-pressure mixing gradient elution method. Applications: This system can be used in a variety of ways, ranging from method development to routine analysis. This system is equipped with a comprehensive set of functions, including a validation function, for supporting conformance to GxP/ISO regulations.
P/N
228-45011-38 228-45001-XX 228-45040-31 228-45019-32 228-45093-93 228-45007-XX 228-45010-XX 228-45004-XX

Description
System Controller Solvent Delivery Unit Low-Pressure Gradient Unit Online Degasser Mixer 0.5-2.6mL HP Auto-Sampler Column Oven UV-VIS Detector LC Workstation

Model Name
CBM-20Alite LC-20AT DGU-20A5 SIL-20AC CTO-20AC SPD-20AV LCsolution Single

Q'ty
1 1 1 1 1 1 1 1 1

I s oc ra t i c S y stem [ Prominence ISO-UV]

Configuration: Basic system that uses the isocratic elution method. Applications: Ideal for applications such as teaching practice. Despite the simple configuration, there is no loss in performance capability and reliable data can be obtained. The system can be upgraded by adding units as required.

P/N
228-45011-38 228-45000-XX 228-45018-32 228-32210-91 228-45009-XX 228-45003-XX

Description
System Controller Solvent Delivery Unit Online Degasser Manual Injecter Column Oven UV-VIS Detector LC Workstation

Model Name
CBM-20Alite LC-20AD DGU-20A3 Rheodyne 7725 CTO-20A SPD-20AV LCsolution Single

Q'ty
1 1 1 1 1 1 1

H i gh - T h ro u g h p u t S y s tem [ Prominence HTLC]

Configuration: Connecting a rack changer to the SIL-20A, which offers high-speed injection and low carryover, makes it possible for this system to perform multi-sample processing. Applications: Ideal for the serial analysis of multiple samples in R&D.

P/N
228-45011-38 228-45002-XX 228-45018-32 228-45093-93 228-45007-XX 228-45030-XX 228-45010-XX 228-45004-XX

Description
System Controller Solvent Delivery Unit Online Degasser Mixer 0.5-2.6mL HP Auto-Sampler Rack Changer C Column Oven UV-VIS Detector LC Workstation

Model Name
CBM-20Alite LC-20AB DGU-20A3 SIL-20AC CTO-20AC SPD-20AV LCsolution Single

Q'ty
1 1 1 1 1 1 1 1 1

B i ol o g i c a l - Sa mp le A n a ly s is S y s t em [ Prominence Co-Sense for BA]

Configuration: Automatically performs complicated sample-pretreatment procedures, such as dilution and concentration. Applications: Incorporates optimum functions for life-science research.

P/N
228-45012-38 228-45000-XX 228-45002-XX 228-45019-32 228-45093-93 228-45007-XX 228-45010-XX 228-45013-91 228-45005-XX

Description
System Controller Solvent Delivery Unit Solvent Delivery Unit Online Degasser Mixer 0.5-2.6mL HP Auto-Sampler Column Oven High-Pressure Flow-Line Selection Valve PDA Detector LC Workstation

Model Name
CBM-20A LC-20AD LC-20AB DGU-20A5 SIL-20AC CTO-20AC FCV-12AH SPD-M20A LCsolution Multi-PDA

Q'ty
1 2 1 1 1 1 1 1 1 1

D ua l H i g h - P re s s u re Gra d ien t S yst em [ Prominence Dual-HGE]

Configuration: This system uses trap injection with two high-pressure gradient flow lines. Applications: Ideal for rapid analysis when used as a front-end HPLC in LC-MS.

P/N
228-45011-38 228-45000-XX 228-45002-XX 228-45018-32 228-45007-XX 228-45010-XX 228-45013-91 228-45015-XX 228-45003-XX

Description
System Controller Solvent Delivery Unit Solvent Delivery Unit Online Degasser Auto-Sampler Column Oven High-Pressure Flow-Line Selection Valve High-Pressure Flow-Line Selection Valve UV-VIS Detector LC Workstation

Model Name
CBM-20Alite LC-20AD LC-20AB DGU-20A3 SIL-20AC CTO-20AC FCV-12AH FCV-20AH2 SPD-20AV LCsolution Multi

Q'ty
2 1 2 2 1 1 2 1 1 1

System Confi g u r a t i o n

List of Units
N e t wo rk s
PC
(Internet Explorer)

S y s tem Cont rollers

CBM-20Alite
(228-45011-38)

P12

CBM-20A

(228-45012-XX)

SCL-10A VP

(228-45051-XX)

Peripheral Devices
(228-45040-91)

P15

Solvent Delivery Units

LC-20AD LC-20AD SP

P14

Aut o- Samplers
Rack Changer C

P16

Low-Pressure Gradient Unit

(228-45000-XX) (228-45091-XX)

(228-45030-XX)

SIL-20A (228-45006-XX) SIL-20AC (228-45007-XX)

Online Degassers DGU-20A 3 DGU-20A 5

(228-45018-32) (228-45019-32)

LC-20AT

(228-45001-XX)

Reservoir Tray
(228-45041-91)

LC-20AB

(228-45002-XX)

SIL-HT A SIL-HT C

(228-45061-XX) (228-45062-XX)

SIL-10AF (228-45056-XX) SIL-10AP (228-45057-XX) SIL-10Ai (228-45075-XX)

Use the CBM-20A or the SCL-10AVP with the SIL-10AF/10AP/10Ai.

Mobile-Phase Selection Valves FCV-7AL (228-45077-91) FCV-11AL (228-45048-91) FCV-11ALS (228-45049-91) FCV-13AL (228-45016-91) FCV-130AL (228-45078-91)

LC-10Ai LC-6AD LC-8A

(228-45089-XX) (228-45068-XX) (228-45069-XX)

Low-pressure gradient units can be incorporated in the LC-20AD and LC-20AT.

10

LC Wo rk s t a t i o n

P24

Dat a Processors
C-R7A plus
(223-04220-XX)

P25

L C s o lu tio n

C-R8A

(223-04500-XX)

Column Ovens
CTO-20A CTO-20AC

P18

D etec to rs
SPD-20A SPD-20AV

P20

Fraction Collector
FRC-10A SPD-M20A (228-45005-XX)
P29 (228-45070-XX)

(228-45009-XX) (228-45010-XX)

(228-45003-XX) (228-45004-XX)

RID-10A RF-10A XL

(228-45095-XX) (228-45096-XX)

CDD-10AVP (228-45054-XX) ELSD-LT@ (228-45115-XX)

Use the CBM-20A or the SCL-10A VP with the FRC-10A.

CTO-10ASVP (228-45059-XX)

The CDD-10AVP can be controlled only with the SCL-10AVP . The ELSD-LT2 and L-ECD-6A output only analog signals. A CBM-20A or a SCL-10AVP and a PC-55N A/D board (223-04202-91) are required for analog signal acquisition.

M S Det ect or Flow-Line Selection Valves

FCV-20AH 2 FCV-20AH 6
P19

P38

LCMS-2020

(225-13300-34)

(228-45015-XX) (228-45017-XX)

FCV-12AH (228-45013-91) FCV-14AH (228-45014-91)

Use the CBM-20A or the SCL-10AVP to control the FCV-20AH6. Two FCV-12AH or FCV-14AH units can be incorporated in the CTO-20A or CTO-20AC. An option box VP (optional: 228-45060-91) is required to connect more units.

Ethernet RS-232C Optical-fiber cable Special cable

The Prominence system is configured with component units. Refer to the relevant page for details on each unit.

11

System Contr o l l e r

CBM-20A
The CBM-20A/CBM-20Alite is a system controller equipped with a data buffering function and acts as an interface for connecting LC workstations, network-client computers, and analytical instruments via Ethernet. Supporting a Variety of Systems

CBM-20A
Up to eight units can be connected to the box-type CBM-20A. Also, adding an optional A/D conversion board makes it possible to accept chromatogram signals from other companies' detectors at LC workstations.

Space-Saving Type

CBM-20Alite
The card-type CBM-20Alite is used by incorporating it into an LC-20Aseries solvent delivery pump or auto-sampler. It can control up to five units, including the unit in which it is incorporated.

Providing a Flexible Installation Environment

CBM-20A/CBM-20Alite units and computers are connected via Ethernet. LAN cables can be used for connection; therefore, analytical instruments and computers can be installed in remote locations. Also, the data buffering function protects important analytical data from unexpected communication errors.
*The data buffering function is available only when using LCsolution.

Achieving Auto-Purge with a Modular HPLC System

The Prominence system is equipped with auto-purge, a function that is normally available only with all-inone HPLC systems. This makes it unnecessary to open or close the drain valve when, for example, replacing the mobile phase. Combining this function with the startup and baseline-check functions enables fully automatic analysis with a modular HPLC system.

12

Equipped with Web-Server Functions

The CBM-20A/CBM-20Alite not only enables connection to computers via Ethernet, but is also equipped with Web-server functions. These allow system control, monitoring, and maintenance management of devices to be performed from Internet Explorer without installing special software.

Checking Device Status at a Glance

It is possible to browse information about the operating status, including the status of devices and the existence of errors, as well as information about device maintenance, including the results of the latest system checks and whether or not consumable parts have been used beyond recommended limits. Also, messages such as "Use next week" or "Perform maintenance" can be communicated between users with the memo function.

The operating status of devices in the network can be checked at a glance.

Control Possible via Network

Devices can be controlled from Internet Explorer without using a special workstation. Execution and monitoring of device conditioning before analysis can be carried out from another room or some other remote location. This makes it unnecessary to keep visiting the laboratory to check on the devices.

Centralized Management of Maintenance Information

Maintenance information, such as the usage frequency of consumable parts in analytical instruments, can be obtained quickly via Internet Explorer. This function is very useful for ensuring the stability and effective use of analytical instruments.

CBM-20A (228-45012-XX) Number of connectable units: 8 Data buffering: Approx. 24 hours (for a sampling rate of 500 ms) Event I/O: 4 inputs, 4 outputs Connectable units: 4 solvent delivery units, 1 auto-sampler, 1 column oven, 2 detectors, 1 fraction collector, 2 sub-controllers CBM-20Alite (228-45011-38) Number of connectable units: 5 (including the unit in which it is incorporated) Data buffering: Approx. 24 hours (for a sampling rate of 500 ms) Event I/O: 2 inputs, 2 outputs Connectable units: 4 solvent delivery units, 1 auto-sampler, 1 column oven, 2 detectors, 2 sub-controllers

13

Solv ent Del i v e r y U n i t

LC-20A
Thanks to drive-system modifications, the LC-20AD/20AT/20AB offers a level of solvent deliver y performance previously unseen. It provides the ideal system for a range of applications from conventional LC to micro LC. Also, incor porating a system controller Superior Solvent Deliver y Perfo r m an ce

LC-20AD
The LC-20AD is a solvent delivery unit that offers the fastest solvent delivery performance in the world. With an automatic pulsationcorrection mechanism and high-speed micro plunger driving, it achieves pulse-free solvent delivery. Thanks to improvements in solvent-delivery control firmware, solvent delivery performance in the micro-flow-rate range below 50 L/min has been significantly improved.

Superior M aint ainabilit y

LC-20AT
The LC-20AT is a solvent delivery unit that possesses a high level of maintainability while delivering high performance. The ability to remove bubbles has been improved by modifying the pump-head structure and the flow line.

Binar y Solvent Deliver y Unit

LC-20AB
The LC-20AB is a binary, high-pressure gradient solvent delivery unit that incorporates two sets of LC-20AD solvent delivery systems. Its space-saving design can be used to create a two-solvent highpressure gradient.

Low-Pressure Gradient Unit

The optional low-pressure gradient unit can be incorporated in the LC-20AD/20AT, enabling gradient elution in a compact space with a small void volume. Automatic matching adjustment of the solenoid valve and pump gives concentration accuracies of 0.5% (LC-20AD) and 1.0% (LC-20AT).

Accurate Gradient Solvent Delivery

By harmonizing two solvent delivery systems, the LC-20AB delivers solvent with an accurate concentration across a wide range from the microflow-rate region to the conventional flow-rate region.

mAU 500 400 300 200 100 0

Set value(%) Measured value(%) 10 20 30 40 50 60 70 80 90 9.98 20.05 30.09 40.03 50.03 60.02 70.01 80.00 90.05

Error -0.157% 0.262% 0.287% 0.069% 0.066% 0.032% 0.011% 0.001% 0.054%

14

(CBM-20Alite) in this unit makes it possible to control and monitor the operating status from PCs in the network, and perform device management for the whole system. Continued Improvements to Solvent Delivery Specifications
The flow-rate accuracy and precision in the microflow-rate region have been improved thanks to the adoption of a new type of check valve and modifications to the solvent-delivery control method. Nonpolar organic solvents, such as hexane, can be delivered stably.
Flow-Rate Accuracy
Set value (mL/min) 0.010 0.050 0.200 1.000 Measured value, n=6 (mL/min) 0.010 0.050 0.201 1.000 Error (%) -1.20% -0.06% 0.43% -0.10% Plunger supported by floating mechanism

Improved Durability
A new type of plunger made with a technique that reflects consideration of the material structure and a precise plunger-holding mechanism help to increase the service life of plunger seals and enable stable solvent delivery over long periods.

Flow-Rate Precision
Set value (mL/min) 0.010 0.050 0.200 1.000 Flow-rate reproducibility, n=6 (RSD%) 0.49 0.08 Plunger seal and center in alignment 0.08 0.01

LC-20AD Solvent delivery method: Parallel-type double plunger, Plunger capacity: 10 L, Maximum discharge pressure: 40 MPa, Flow-rate setting range: 0.0001 to 10.0000 mL/min LC-20AT Solvent delivery method: Series-type double plunger, Plunger capacity: 47L on primary side, 23 L on secondary side, Maximum discharge pressure: 40 Mpa, Flow-rate setting range: 0.001 to 10.000 mL/min LC-20AB Solvent delivery method: Parallel-type double plunger (2 sets), Plunger capacity: 10 L, Maximum discharge pressure: 40 MPa, Flow-rate setting range: 0.0001 to 10.0000 mL/min Gradient LC-20AB Gradient type: High-pressure mixing, Number of mixed solvents: 2, LC-20AB high-pressure GE system LC-20AD/20AT Gradient type: High-pressure mixing, Number of mixed solvents: 2 or 3, LC-20AD/20AT high-pressure GE system LC-20AD/20AT Gradient type: Low-pressure mixing, Number of mixed solvents: 4 max, LC-20AD/20AT low-pressure GE system

DG U - 2 0 A 3 / 2 0 A 5 D eg as s e r
The DGU-20A 3 / 20A 5 is an on-line degasser that uses fluoroethylene membrane. The internal capacity is small at 0.4 mL, only 1/25th of that for existing Shimadzu models, and the waiting time at mobilephase replacement or stabilization can be significantly reduced. The degassing efficiency has also been improved, ensuring thorough degassing even at high flow rates.
Number of degassed solvents for DGU-20A 3 : 3 Number of degassed solvents for DGU-20A 5 : 5

15

Aut o-S am pl e r

SIL-20A
The SIL-20A/20AC offers greater analysis efficiency, achieved through improvements in sample injection speed and processing capacity. Also, comprehensive sample Suppor t ing High- Throughput Analysis

S IL-20A
The SIL-20A is a total-volume injection-type auto-sampler that enables high-speed injection and multi-sample processing. It was designed to ensure greater stability, with improved durability attained through modifications in valves and sample loops.

Equipped wit h Cooling Funct ion

S IL-20AC
The SIL-20AC is equipped with a sample cooler that incorporates a dehumidifying function. Samples can be maintained at a fixed temperature in the range of 4 C to 40 C. The high cooling speed makes it possible to keep easily decomposed sample constituents in a stable condition.

Carryover Reduced to an Absolute Minimum

Adsorption of sample constituents has been reduced to an absolute minimum by using a special processing technology for the sampling needle (patent pending) and rethinking the structure of the needle seal and the materials used in flow-line parts. As a result, there is hardly any sample carryover. Also, the adoption of a PEEK rotor seal allows use over a wide pH range, from strongly acidic conditions to strongly basic conditions. Using the optional rinse kit (228-43042-91) makes it possible to rinse the sampling needle with two different solvents, selected in accordance with the purpose.

Easily adsorbed compounds can be handled with the SIL-20A.

0.0008 0.0007 0.0006 0.0005 0.0004 0.0003 0.0002 0.0001 0.0000 -0.0001 -0.0002 0 1 2 3 4 0.0008 0.0007 0.0006 0.0005 0.0004 0.0003 0.0002 0.0001 0.0000 -0.0001 -0.0002 7 8 9 10 0 1

They are hardly detected at all with a rinsing pump.

0.0007%

Not detected

5 min

5 min

10

Sample Carryover Test for Chlorhexidine

16

carryover countermeasures make it possible to perform analysis without carr yover, even in high-sensitivity LC-MSMS. High Throughput
Only 10 seconds* are required for sample injection. High-speed vertical motion of the needle enables ultra-high speed sample processing, which was considered impossible with conventional auto-samplers. Using in combination with a high-speed-separation column makes an analysis cycle of less than one minute a reality.
0 0.5 1 1.5 2 2.5 3 min

*10 L injection

Precise Sample Injection

Injection-Volume Accuracy
Set value (L) 1 2 5 10 20 50 100 Measured value (L) 0.99 1.99 5.01 10.00 19.92 49.90 99.70 Error (%) -0.90 -0.70 0.20 0.00 -0.40 -0.20 -0.30

Injection-Volume Precision
Injection volume (L) 1 2 5 10 20 50 100 Area reproducibility (%RSD) 0.43 0.25 0.06 0.04 0.03 0.10 0.11

Greater accuracy has been attained by incorporating a high-performance sampling device that can measure out the samples with high precision. The design reflects an emphasis on basic performance as well as functionality. Also, using direct injection means valuable samples are not wasted.

SIL-20A

Injection method: Total-volume sample injection, variable injection volume Injection-volume setting range: 0.1 to 100 L (standard), 1 to 2,000 L (option) Number of processed samples: 175 (1 mL vials), 105 (1.5 mL vials), 50 (4 mL vials), two 96-well MTPs, two 384-well MTPs, and ten 1.5 mL vials in addition to these. SIL-20AC Injection method: Total-volume sample injection, variable injection volume Injection-volume setting range: 0.1 to 100 L (standard), 1 to 2,000 L (option) Number of processed samples: 175 (1 mL vials), 70 (1.5 mL vials), 105 (1.5 mL vials), 50 (4 mL vials), two 96-well MTPs, two 384-well MTPs, and ten 1.5 mL vials in addition to these. *A sample rack for 1.5mL vials and a control vial rack are included in the SIL-20A/20AC as a standard accessory.

Rack Changer: Increasing the Number of Processed Samples

A rack changer is an optional product that can be used to change the microplates in the auto-sampler's racks and thereby facilitate serial analysis. Up to 12 plates can be mounted in the rack changer. This model incorporates a cooling function. When the samples have been prepared, simply set them in the rack changer to perform continuous sample processing.
Rack changer C (228-45030-XX) Compatible plates: 96-well MTPs, 96-well DWPs 384-well MTPs, 384-well DWPs 1.5mL vial plate (54 vials) Number of processed plates: 12 Sample cooler: Block cooling/heating, used together with dehumidifying function, 4C to 40C

17

Colu m n O ven

CTO-20A
The CTO-20A/20AC precisely regulates the temperature around the column and suppor ts stable analysis that is not influenced by the ambient temperature. In addition to the column, various other par ts and units can be accommodated, including Accommodat ing M ult iple Colum n s

CTO-20A
The CTO-20A is a forced air-circulation-type column oven. It can regulate the temperature in a range going from 10 C above room temperature to 85 C. It also allows the setting of complex temperature programs by incorporating, for example, linear or step-wise increases and decreases in temperature.

Cooling Also Suppor t ed

CTO-20AC
The CTO-20AC model is equipped with a cooling function. Using an electronic cooler, it can regulate the temperature in a large range going from 10 C below room temperature to 85 C.

Precise Temperature Regulation

The interior of the oven is precisely regulated with a high-performance thermistor. Also, the temperature is calibrated at two different temperatures to ensure a high level of temperature accuracy.
Changes in Room Temperature and Internal Oven Temperature in One Day 30
Room temperature Internal oven temperature

Changes in Room Temperature and Stability of Retention Time for Vitamin A Acetate in One Day 50
Retention time

Retention time (min)

Temperature (C)

48 46 44 42

25

RSD 0.052% (n=24)

20 2004/6/28 12:00

2004/6/28 18:00

2004/6/29 0:00

2004/6/29 6:00

2004/6/29 12:00

40 2004/6/28 12:00

Analysis time (hours, minutes)

2004/6/28 2004/6/29 2004/6/29 18:00 0:00 6:00 Analysis time (hours, minutes)

2004/6/29 12:00

18

a manual injector, gradient mixer, high-pressure flow-line selection valves (2-position/6por t valves or 6-position/7-por t valves, two in total), a conductivity-detection cell block, and a reaction coil. Installation of CMD (Option)
This product supports installation of the CMD (Column Management Device), which can be used to record information about the way the column is used, such as the number of injections, the amount of mobile phase that flows, and the composition of the last mobile phase used. This information can be managed at an LC workstation (LCsolution / CLASSVP) or a PC in the network using the Web-control function.

Incorporation of Flow-Line Selection Valve

The FCV-12AH/14AH high-pressure flow-line selection valve can be incorporated and controlled. Position display is also possible.

Temperature-control method: Forced air-circulation Cooling method: None Temperature setting range: 4C to 85C Temperature-control precision: 0.1C max. (typically 0.04C max.) Temperature-control range: 10C above room temperature to 85C CTO-20AC Temperature-control method: Forced air-circulation Cooling method: Electronic cooling Temperature setting range: 4C to 85C Temperature-control precision: 0.1C max. (typically 0.04C max.) Temperature-control range: 10C below room temperature to 85C CTO-20A

FCV-20AH 2 /20AH 6 Flow-Line Selection Valves

The FCV-20AH2/20AH6 is a stand-alone, high-pressure, flow-line selection valve. The valve position is controlled by event signal input. Direct control is also possible from the unit itself.
FCV-20AH2 Valve type: 2-position/6-port rotary valve Maximum operating pressure: 39.6 MPa (400 kg /cm2) Operating pH range: pH1 to pH10 Operating temperature range: 4C to 35C FCV-20AH6 Valve type: 6-position/7-port rotary valve Maximum operating pressure: 39.6 MPa (400 kg /cm2) Operating pH range: pH1 to pH10 Operating temperature range: 4C to 35C

19

Abs or bance D e t e c t o r

SPD-20A
The SPD-20A/20AV/M20A is an absorbance detector that offers a high level of sensitivity and stability. The lineup consists of the SPD-20A/20AV dual-wavelength absorbance Highest Level of Sensitivity in the World UV- VIS Det ect or

S P D-20A / 20AV
The SPD-20A/20AV is a UV-VIS detector that takes sensitivity to the limit. It has a noise level of 0.5 x 10 -5 AU max., making it one of the most sensitive models of its kind in the world. The SPD-20AV has a mode that allows the deuterium lamp and tungsten lamp to be lit simultaneously, enabling high-sensitivity wavelength-programming detection for ultraviolet light and the entire visible-light range.

Sensitivity Levels Approaching Those of UV-VIS Detectors PDA Det ect or

S P D-M20A
The SPD-M20A also has a high-sensitivity mode. Using light-source compensation, it offers a sensitivity level that, at 0.6 x 10 -5 AU, is comparable to that of UV-VIS detectors.

Superior Linearity
Using newly developed signal processing technology, the stray-light correction function has been enhanced, and the linearity has been improved to a point where it satisfies the following ASTM standards: SPD-20A/20AV : 2.5AU SPD-M20A : 2.0AU This product ensures greater analysis precision in, for example, purity tests.
AU 2.5 2.0 1.5 2 1.0 0.5 1.5 0.0 0 1 AU 0.004 0.5 0 0 50 100 150 0.003 0.002 0.001 0.000 2.0 2.5 3.0 3.5 4.0 4.5 min 0.012% 0.127% 0.117% 1 2 3 4 5 6 min

Concentration Linearity for Drug Substance A and Purity Test

20

detector and the SPD-M20A photodiode array detector. They are equipped with temperature-controlled flow cells to increase the peak-response and baseline stability. Browser Control Supported
The SPD-M20A is equipped with an Ethernet interface and Web-server functions, allowing devices to be monitored and controlled from PCs in the network. Also, as a stand-alone, high-sensitivity, multi-wavelength detector, it can be incorporated into other companies' HPLC systems.

Consideration Given to Validation

The incorporation of a low-pressure mercury lamp for wavelength calibration ensures simple calibration in the ultraviolet region.

Greater Stability Achieved with a Temperature-Controlled Flow Cell

The SPD-20A/20AV and SPD-M20A are equipped with a temperature-controlled flow cell as a standard feature. This helps increase the baseline stability and the analysis reliability.
Without Cell-Temperature Control mAU C 40
Cell temperature

Cell Temperature Controlled at 40C mAU C 40

Cell temperature

35 30 25 15 10 5

35 30 25 20

15 10

Room temperature

Room temperature

Baseline

20
Baseline

5 0 0 30 60 90 120 min

0 0 30 60 90 120 min

Effect of Temperature-Controlled Flow Cell: Behavior of Baseline When Room Temperature is Changed from 20C to 30C

SPD-20A Light source: Deuterium (D2) lamp Wavelength range: 190 to 700 nm Bandwidth: 8 nm Wavelength accuracy: 1 nm max. SPD-20AV Light source: Deuterium (D2) lamp, tungsten (W) lamp Wavelength range: 190 to 900 nm Bandwidth: 8 nm Wavelength accuracy: 1 nm max. SPD-M20A Light source: Deuterium (D2) lamp, tungsten (W) lamp Number of diode elements: 512 Wavelength range: 190 to 800 nm Slit width: 1.2 nm (high-resolution mode), 8 nm (high-sensitivity mode) Wavelength accuracy: 1 nm max. Optional cells for SPD-20A/20AV: Semi-micro temperature-controlled flow cell (228-45605-93), micro-cell (228-25293-92), inert cell (228-33338-91), and other types of preparative cells. Optional cell for SPD-M20A: Semi-micro temperature-controlled flow cell (228-45605-94), micro-cell (228-25293-93), inert cell (228-34187-91), and other types of preparative cells.

21

Spe ci fi cati on s

Specifications for Prominence Syste

CBM-20A / 20Alite
S ys tem C ont roller
Connectable units Number of connectable units Data buffering Event I/O Analog boards Operating temperature range Dimensions, weight Power requirements 260 (W) x 140 (H) x 420 (D) mm, 5.5 kg AC 110V, 230V, 100VA, 50/60 Hz CBM-20A (228-45012-XX) Solvent delivery units: 4 max.; Auto-samplers: 1, Column ovens: 1; Detectors: 2 max.; Fraction collectors: 1; Sub-controllers: 2 max. 8 (expansion possible up to 12) 4 inputs, 4 outputs Up to 2 boards can be mounted. 4C to 35C 120 (W) x 20 (H) x 100 (D) mm, 0.5 kg Supplied from unit CBM-20Alite (228-45011-38) Solvent delivery units: 4 max.; Auto-samplers (SIL-10AF/10AP/10Ai): 1; Column ovens: 1; Detectors: 2 max.; Sub-controllers: 2 max.

P12

5 (including the unit incorporating the system controller) Approx. 24 hours for one analysis (at 500-ms sampling rate; available only with LCsolution) 2 inputs, 2 outputs Mounting not supported.

LC-20AD / 20AT / 20AB

S o lv e n t D eliver y Unit
LC-20AD (228-45000-XX) Solvent delivery method Plunger capacity Maximum discharge pressure Flow-rate setting range Flow-rate accuracy Flow-rate precision Typical pulsation Constant-pressure solvent delivery Plunger rinsing mechanism Safety measures Operating temperature range Dimensions, weight Power requirements Gradient System LC-20AB Gradient type Number of mixed solvents Mixing-concentration accuracy Mixing-concentration precision High-pressure mixing 2 0.5% max. 260 (W) x 140 (H) x 420 (D) mm,10 kg 0.0001 mL/min to 10.0000 mL/min No more than 1% or 2L/min, whichever is greater (0.01 to 2 mL/min) Parallel-type double plunger 10L LC-20AT (228-45001-XX) Series-type double plunger Primary side: 47L,Secondary side:23L 40MPa 0.001 mL/min to 10.000 mL/min No more than 2% or 2L/min, whichever is greater (0.01 to 5 mL/min) 10L

P14
LC-20AB (228-45002-XX) Parallel-type double plunger (2 sets)

0.0001 mL/min to 10.0000 mL/min No more than 1% or 2L/min, whichever is greater (0.01 to 2 mL/min) 0.03 MPa (for water at 1.0 mL/min and 7 MPa)

No more than 0.06% RSD or 0.02 min SD, whichever is greater 0.03 MPa (for water at 1.0 mL/min and 7 MPa) 0.08 MPa (for water at 1.0 mL/min and 7 MPa) Supported Manual rinsing or automatic rinsing using optional product Liquid-leakage sensor, high-pressure/low-pressure limits 4C to 35C W 260 x H 140 x D 420 mm, 11 kg W 260 x H 140 x D 420 mm, 13 kg AC 110V, 230V, 180VA, 50/60Hz AC 110V, 230V, 150VA,50/60Hz

LC-20AD/20AT, high-pressure GE specifications LC-20AD/20AT, low-pressure GE specifications High-pressure mixing 2 or 3 0.1% RSD max. Low-pressure mixing 4 max.

0.5% max. (LC-20AD), 1.0% max. (LC-20AT)

DGU-20A 3 / 20A 5

O n lin e D egasser
DGU-20A3 (228-45018-32) Number of degassed solvents Degassed flow-line capacity Operating temperature range Dimensions, weight Power requirements 260 (W) x 70 (H) x 420 (D) mm, 5 kg 3 380L 4C to 35C 260 (W) x 70 (H) x 420 (D) mm, 5.2 kg DGU-20A5 (228-45019-32) 5

P15

Supplied from LC-20AD/20AT/20AB

SIL-20A / 20AC

Au to -S a mpler
Injection method Injection-volume setting range SIL-20A (228-45006-XX) SIL-20AC (228-45007-XX) Total-volume sample injection, variable injection volume 0.1L to 100L (standard),1L to 2,000L (option) 175 (1 mL vials) 105 (1.5 mL vials) Number of processed samples 50 (4 mLvials) 192 (two 96-well MTPs) 768 (two 384-well MTPs) Also, ten 1.5 mL vials in addition to each of the above. Injection-volume accuracy Injection-volume precision Cross-contamination Number of repeated injections Needle rinsing Sample cooler Operating pH range Operating temperature range Dimensions, weight Power requirements 260 (W) x 415 (H) x 500 (D) mm, 27 kg AC 110V, 230V, 100VA, 50/60 Hz None 1% max. RSD: 0.3% max. (typically 0.2% RSD max., for 10L injection, under specified conditions) 0.005% max. (naphthalene, chlorhexidine, typically 0.0025% max.) 30 max. per sample Set freely before and after sample injection.

P15

175 (1 mL vials) 70 (1.5 mL vials) 105 (1.5 mL vials) 50 (4 mLvials) 192 (two 96-well MTPs) 768 (two 384-well MTPs) Also, ten 1.5 mL vials in addition to each of the above.

Block cooling/heating, used together with dehumidifying function, 4C to 40C pH1 to pH14 4C to 35C 260 (W) x 415 (H) x 500 (D) mm, 30 kg AC 110V, 230V, 300VA, 50/60 Hz

22

em
Rack Changer
R ack C h a ng er
Rack changer C (228-45030-XX) Compatible plates Number of processed plates Sample cooler Operating temperature range Dimensions, weight Power requirements 96-well MTP, 96-well DWP, 384-well MTP, 384-well DWP, 1.5mL vial plate (54 vials) 12 Block cooling/heating, used together with dehumidifying function, 4C to 40C 4C to 35C 425 (W) x 415 (H) x 500 (D) mm, 32 kg AC 110V, 230V, 350VA, 50/60 Hz

P17

CTO-20A / 20AC

C o lu mn Oven
CTO-20A (228-45009-XX) Temperature-control method Cooling method Temperature setting range Temperature-control precision Temperature-control range Storage capacity Storable devices Time program Safety measures Operating temperature range Dimensions, weight Power requirements 260 (W) x 415 (H) x 420 (D) mm, 20 kg 10C above room temperature to 85C None 4C to 85C 0.1C max. (typically 0.04C max.) 10C below room temperature to 85C 220 (W) x 365 (H) x 95 (D) mm 2 manual injectors, gradient mixer, 2 high-pressure flow-line selection valves, etc. Linear temperature programs supported. Solvent sensor, temperature fuse, temperature upper limit 4C to 35C 260 (W) x 415 (H) x 420 (D) mm, 23 kg AC 110V, 230V, 600VA, 50/60 Hz Forced air-circulation Electronic cooling CTO-20AC (228-45010-XX)

P18

FCV-20AH 2 /20AH 6

H ig h -P re s sure Flow- Line Select ion Valve

FCV-20AH2 (228-45015-XX) Valve type Maximum operating pressure Operating pH range Operating temperature range Dimensions, weight Power requirements 110 (W) x 140 (H) x 250 (D) mm, 4 kg 2-position/6-port rotary valve 39.6 MPa (400 kg/cm2) pH1 to pH10 4C to 35C 110 (W) x 140 (H) x 250 (D) mm, 4 kg AC 110V, 230V, 100VA, 50/60 Hz FCV-20AH6 (228-45017-XX) 6-position/7-port rotary valve

P19

S PD -2 0 A / 2 0A V S PD -M 2 0 A

UV-VIS Detectors PDA Detector

Light source Number of diode elements Wavelength range Bandwidth, slit width Wavelength accuracy Wavelength precision Noise Drift Linearity

Absorbance Det ect or

SPD-20A (228-45003-XX) Deuterium (D2) lamp None 190 nm to 700 nm 8 nm 1 nm max. 0.1 nm max. 0.5 x 10-5 AU (under specified conditions) 1 x 10-4 AU/h (under specified conditions) 2.5 AU (ASTM standard) Dual-wavelength detection in the range 190 to 370 nm and upwards of 371nm, ratio-chromatogram output, wavelength scanning 190 nm to 900 nm SPD-20AV (228-45004-XX) SPD-M20A (228-45005-XX) Deuterium (D2) lamp, tungsten (W) lamp 512 190 nm to 800 nm 1.2 nm (high-resolution mode), 8 nm (high-sensitivity mode)

P20

0.6 x 10-5 AU (under specified conditions) 5 x 10-4 AU/h (under specified conditions) 2.0 AU (ASTM standard) Contour output, spectrum library, MAX plotting Optical wavelength: 10 mm, Capacity: 10L, Pressure: 12 MPa Parameter setting, log management, management of consumable parts,etc.

Functions

Cell Cell temperature-control range Web control

Optical wavelength: 10 mm, Capacity: 12L, Pressure: 12 MPa

5C above room temperature to 50C

Buffer memory Operating temperature range Dimensions, weight Power requirements

Refer to the information on the CBM-20A/Alite 4C to 35C 260 (W) x 140 (H) x 420 (D) mm, 13 kg AC 110V, 230V, 160VA, 50/60 Hz

Approx. 20 minutes of data in the entire wavelength region (only when using LCsolution)

260 (W) x 140 (H) x 420 (D) mm,12 kg AC 110V, 230V, 150VA, 50/60 Hz 23

Sof tw ar e

LCsolution
[ LC Workstation ]

Advanced LC Workstation

LCsolution
The LCsolution software package provides total support for analysis work, including hardware control of the Prominence series, the LC-VP series and the LC-8A/6AD pumps. Data acquisition, report generation, and data management are standard features. The control software helps reduce the workload involved in analysis by automating all procedures from instrument conditioning to shutdown. These workstations also offer the functions required for ensuring the reliability of analysis data with respect to issues such as security and audit trails, which are demanded in analysis performed under regulations such as GLP/GMP or FDA 21 CFR Part 11.

Increasing the Efficiency of Analysis Work

Analysis workflow consists of a series of procedures starting from instrument conditioning and judgment of instrument stability, evaluation of sample results, and shutdown after analysis. The automation functions offered by the combination of Prominence systems with LCsolution cover all of these procedures, thereby reducing the workload required to perform analyses. They also make it possible for anyone, even inexperienced operators, to perform analysis under stable conditions.

Starting Instruments

Shutdown

Instrument Condition Check

Startup
Instrument operation is started at a specified time.

Cooldown
After analysis is completed, appropriate cooldown is performed to protect the column.

Intelligent Instrument Control

automation
System Check
Ensures the hardware system is working properly prior to injecting the sample.

Action
Generation of calibration curves, system suitability tests, and judgments of the samples analysis results are automatically performed. The instrument is controlled intelligently with the action functions.

Purge of Mobile Phase

Instrument Stability Check

Autopurge
The mobile phase and the autosampler's rinse solvent are purged automatically.

Baseline Check
Judgments about column equilibrium and the stability of the instrument are made from noise and drift measurements after the start of solvent delivery.

24

LCsolution Licensing Information

LCsolution Single LCsolution Multi LCsolution Multi-PDA Data acquisition and instrument control of a single Prominence/LC-VP system via CBM-20A, CBM-20Alite, SCL-10AVP or SIL-HT, or a single LC-2010/HT system. Data acquisition and instrument control of up to four Prominence/LC-VP systems via CBM-20A, CBM-20Alite, SCL-10A VP or SIL-HT, or up to four LC-2010/HT systems. Data acquisition and instrument control of up to four Prominence/LC-VP systems via CBM-20A, CBM-20Alite, SCL-10A VP or SIL-HT, or up to four LC-2010/HT systems. Capable of control of up to two SPD-M20A detectors.

Optional Software
LCsolution Postrun Kit Using the LCsolution Postrun Kit makes it possible to re-analyze the data accumulated by LCsolution at another PC.

Increasing the Efficiency of Data Management

CLASS-Agent
Database management provides an effective way of managing important analysis data safely over long periods of time and accessing it quickly when needed. CLASS-Agent makes this possible by allowing analysis data and information about the conditions at the time of analysis to be managed securely in a database. The CLASS-Agent Network System can be connected to various types of analytical instruments, as well as to LCsolution and CLASS-VP, and helps achieve total data management for laboratories that effectively utilize network resources.

C h ro m a t o p a c : Chromatography Data Processors

The Chromatopac, which was developed specifically as a device for processing chromatography data, is an integrator that also offers the basic functions of a data processor in an easy-to-use format.

C-R7A plus

(223-04220-XX)

C-R8A

(223-04500-XX)

Compact, high-speed parallel printer Chromatopac BASIC well-suited to system automation Highly reliable optical link control Supports GLP, GMP, and ISO-9000 Sliding display screen saves space

Inherits data processing capability with established reputation and simple operability Equipped with automatic validation functions Handles high-speed RS-232C as a standard feature Simple operation optimized for factory use

25

Pr omi nence C o m p a t i b l e U n i t s

SCL-10AVP
[ System Controller ]

Supports a Wide Variety of System Configurations

The SCL-10A VP performs centralized control of all Prominence* and LC-VP series modules, and the LC-8A/6AD solvent delivery units. It also operates as an interface with LC workstations.
*Prominence modules are operated in VP compatible mode.

Customization Functions for Improving Operability

This controller is equipped with customization functions that allow parameters that are often used in time programs, for example, to be selected and displayed in a single screen. Clearly defined characters and a graphical function-key menu contribute to intuitive operation. With isocratic systems, it is possible to select "simple mode", in which only minimal operation parameters are displayed. For sample preparation such as dilution or reagent addition using autosampler pretreatment, it is possible to select either simple mode (Quick-pret) or a detailed setting mode (Pret-prog, Advanced).

Validation Support
From the maintenance screen, it is possible to load, display, and output operation logs and maintenance information for connected modules.

Specifications
Display

(SCL-10A VP )
SCL-10AVP (228-45051-xx) Backlit LCD (320 x 240 dots) Solvent delivery unit: 3 max.; Autosampler: 1; Column oven: 1; Detector: 2 max.; Fraction collector: 1; 2- or 6-position valve: 2 max.

Connectable units

(via Option Box VP or Sub-controller VP); Solenoid valve unit: 1(via Sub-controller VP or solvent delivery unit); Helium degasser: 1 (via Sub-controller VP or solvent delivery unit)

Solvent delivery unit control Autosampler control Column oven control Detector control Fraction collector control Input/Output terminals Analog boards Ambient temperature range Dimensions, weight Power requirements
26

Solvent delivery control modes: Isocratic, High-pressure gradient, Low-pressure gradient, Constant-pressure delivery Programmable parameters: Flow rate, Pressure, Concentration, Max. pressure, Min. pressure, Linear, Step, Exponential function (all multi-level) Sample injection volume, Number of repetitions of injections, Analysis time or analysis file number, Pretreatment file number,Fraction collector file number Oven temperature, Max. temperature Detection wavelength, Range, Time constant, Lamp switching, Wavelength scanning conditions, etc. 15 types of fractionation conditions, Time programs (peak-detection fractionation, high-purity fractionation based on 2-signal processing, etc.) External start input: 1; Error input: 3 General-purpose output: 4; External power switch control: 1; Optical link for Chromatopac: 1; Optical link for unit control: 8; RS-232C (for PC): 1 2 max. (Option) 4C to 35C 260(W) x 140(H) x 420(D)mm, 6kg AC 110V, 230V, 320VA, 50/60Hz

P r omi nence C o m p a t i b l e U n i t s

LC-10Ai / 6AD / 8A
[ Solvent Delivery Units ]

Solvent Delivery Unit for Bio-inert HPLC System

LC-10Ai
This bio-inert solvent delivery unit incorporates a serial dual plunger and offers lowpulsation performance from an optimized cylinder volume. It can be used together with the SIL-10Ai and SPD-20A (with bio-inert cell) to construct a high-performance bio-inert LC system. The LC-10Ai uses PEEK resin in liquid contact parts and is ideal for the analysis of physiologically active substances and metal ions. Resistance to acids, bases, and highconcentration aqueous NaCl solutions is even higher than with stainless steel.

Solvent Delivery Unit for Analytical to Semi-Preparative Scale

LC-6AD
This multi-purpose pump delivers highly accurate solvent flow in a range from the low flow rate region (< 1 mL / min) to semi-preparative flow rates (up to 20 mL / min). When used with the 6AD recycle kit, it achieves a very high level of recycling efficiency for semi-preparative columns. Depending on conditions, more than one million theoretical plates can be obtained.

Solvent Delivery Unit for Large-Scale Preparative Work

LC-8A
This solvent delivery unit is specifically designed for preparative separations and can perform solvent delivery at high flow rates (up to 150mL / min). With high flow rate accuracy in the analytical region (1-2 mL / min), it is easy and convenient to use the same pump to perform large-scale separation with a preparative column ( inner diameter: 20 to 50mm) after studying analytical conditions with a standard-size analytical column.

Specifications
Solvent-delivery method Plunger capacity Maximum discharge pressure Flow-rate setting range Flow-rate accuracy Flow-rate precision Constant-pressure delivery Plunger rinse mechanism Ambient temperature range Dimensions, weight 260 Power requirements

(LC-10Ai / 6AD / 8A)

LC-10Ai (228-45089-xx) Serial dual plunger Primary side: 47L; Secondary side: 23L 27.4MPa 0.001 to 9.999mL/min Within 2% or 2L/min., whichever is larger (0.1 to 5.0mL/min.) 0.3% max.(RSD: 0.1% max.) Possible Syringe or rinsing pump (228-39625-91) used 4C to 35C 260(W) x 140 (H) x 420 (D)mm, 10kg AC 110V, 230V, 100VA, 50/60Hz LC-6AD (228-45068-xx) Parallel dual plunger 47L 49.0MPa 0.01 to 20.00mL/min 0.001 to 9.999mL/min (with switch selection) Within 1% or 10L/min., whichever is larger (0.01 to 5.0mL/min.) 0.3% max.(RSD: 0.1% max.) Possible Syringe or FCV-7AL (228-45077-91) used 10C to 40C 260(W) x 160(H) x 500(D)mm, 20kg AC 110V, 230V, 200VA, 50/60Hz LC-8A (228-45069-xx) Parallel dual plunger 280L 29.4MPa 0.1 to 150.0mL/min Within 2% (0.5 to 150.0mL/min.) 0.5% max. Possible Syringe or FCV-130AL (228-45078-91) used 10C to 35C 350(W) x 210(H) x 450(D)mm, 32kg AC 110V, 230V, 750VA, 50/60Hz

* A PC-31L interface (228-31103-91) must be installed in the LC-6AD or the LC-8A to enable connection of the solvent delivery unit to the CBM-20A/Alite or SCL-10AVP system controller. * System-check software cannot be used with the LC-6AD or the LC-8A. * Use the 8A preparative mixer (228-20600-91) if the flow rate is greater than or equal to 10mL/min.
27

Pr omi nence C o m p a t i b l e U n i t s

SIL-10AF / 10AP / 10Ai / HT

[ Autosamplers ]

Versatile Autosamplers

SIL-10AF / 10AP
The SIL-10AF and SIL-10AP injectors use the fixed-loop injection method. They can also perform sample pretreatment, including dilution and mixing, at a high speed. The SIL-10AP is a preparative autosampler that can inject up to 5mL while offering the same level of performance and functionality as the SIL-10AF.

Bio-inert Autosampler

SIL-10Ai
This bio-inert autosampler uses PEEK resin in parts that contact liquids. It can be used for the analysis of physiologically active substances and metal ions.

Sample Racks for SIL-10AF / 10AP / 10Ai

Sample rack S (228-21046-91) for 1.5mL vials Sample rack MTP2* (228-40460-91) for 96-well standard/deep-well microtiter plates Reagent rack (228-20905) for 15mL reagent vials Sample rack L (228-21046-92) for 4.0mL vials Sample rack LL* (228-39384-91) for 13mL vials * Cannot be used with SIL-10Ai.

High-Throughput Autosamplers

SIL-HTA / HTc
The SIL-HT autosampler enables ultra high-speed sample injection and multi-sample processing. It achieves excellent repeatability and near-zero sample carryover, and is ideal for the front-end of an LC-MS system. A Peltier sample cooler and built-in dehumidifier maintain sample temperatures from 4-40 C in the SIL-HTc.

Specifications (SIL-10AF/10Ai /10AP/ SIL-HT A/ HT C)

SIL-10AF (228-45056-xx) Injection method 1L to 50L (standard) 1L to 400L (option) 1L to 2,000L (option) 1L to 5,000L (option) SIL-10Ai (228-45075-xx) SIL-10AP (228-45057-xx) SIL-HTA (228-45061-xx) SIL-HTC (228-45062-xx) Loop injection, variable injection volume 1L to 50L (standard) 1L to 250L (option) 1L to 5,000L (standard) 1L to 400L (option) 1L to 2,000L (option) Total-volume injection, variable injection volume 0.1L to 100L (standard) 1L to 2,000L (option)

Injection volume setting range

100 with 1.5mL vials (60 with optional cooler) Number of samples processed 80 with 4mL vials (50 with optional cooler) 25 with 13mL vials (not applicable to SIL-10Ai) 192 with two 96-well microtiter plates Injection volume accuracy Injection-volume precision Sample Carryover Number of repeated injections Needle rinsing Sample cooler Operating pH range Operating temperature range Dimensions, weight Power requirements
28

350 with 1mL vials 210 with 1.5mL vials (140 with SIL-HTC) 100 with 4mL vials 384 with four 96-well microtiter plates 1,536 with four 384-well microtiter plates 1% max. (for 50L injection) RSD: 0.3% max. (10L injection, under specified conditions) 0.01% max. (caffeine) 99 max. per sample Block-heating /cooling method Used together with dehumidifying function 4C to 40C pH 1 to pH 14 4C to 35C

Not specified RSD: 0.5% max. (10L injection, standard mode) Not specified 30 max. per sample Set freely before and after sample injection Optional Sample Cooler S (228-45063-xx) or L (228-45064-xx) Block-heating /cooling method 4C to 70C pH 1 to pH10 Main unit : 260(W) x 280(H) x 420(D)mm, 19kg Syringe unit : 100(W) x 280(H) x 150(D)mm, 4kg AC 110V, 230V, 100VA, 50/60Hz RSD: 1% max. (10L injection)

None

540(W) x 415(H) x 500(D)mm, 40kg(SIL-HTA), 45kg(SIL-HTC) AC 110V, 230V, 150VA, 50/60Hz AC 110V, 230V, 300VA, 50/60Hz

P r omi nence C o m p a t i b l e U n i t s

FRC-10A
[ Fraction Collector ]

A Fraction Collector for a Wide Variety of Fractionation Modes

The FRC-10A can be used over a wide range of flow rates, covering small and large-scale preparative work. It flexibly adapts to various applications, such as simple, manual collection performed while viewing chromatograms, and advanced, continuous and automated preparative separation and collection performed in combination with an autosampler and detector.

Convenient Fraction Simulation

Fraction simulation can be performed using LCsolution, so the optimization of fractionation conditions is very simple.

Minimal Influence of Variations in Elution Time

Even if the elution time changes due to the influence of fluctuations in room temperature or the composition of the mobile phase, it is still possible to accurately perform fractionation by catching the target component with special parameters. This function is indispensable for continuous automatic preparative separation.

Options
Fraction collector heads, racks, and collection tubes and vials are available as options. Select according to fraction size required.
Collector heads Large-scale fractions Fraction collector head with valve (228-24105-91) Racks Large- volume kit (228-25324-91) Rack No. 3: 16 fractions (228-25313-91) Collection containers Commercial reagent bottles (500 to 1,000mL) can be used.

Semi-large fractions

50mL vials (glass, 20 pcs / set) (228-25318-91)

50mL vials (PP (polypropylene), 20 pcs / set) (228-25321-91) 25mL test tube (PP, 100 pcs / set) (228-25320-91)

Rack No. 2A: 64 fractions 20mL test tube (glass, 100 pcs / set) (228-25311-91) (228-25316-91) Sample cooler L: 50 fractions (228-24975-93)

32mL test tube (glass, 100 pcs / set) (228-25317-91)

5L vials (glass, 100 pcs / set) (228-21287-91) 3.5mL test tube (glass, 250 pcs / set) (228-25315-91) 3.5mL test tube (glass, 250 pcs / set) (228-25315-91)

5mL vials (PP, 100 pcs / set) (228-21322-91) 4.5mL test tube (PP, 250 pcs / set) (228-25319-91) 4.5mL test tube (PP, 250 pcs / set) (228-25319-91)

Small fractions

Rack No. 1: 144 fractions (228-25310-91) Fraction- collector head Rack No. 5: 120 fractions (228-25314-91) (228-25169-91)

* A "fraction-collector head with valve" allows the eluate to be switched between the fraction side and the drain side using a 3-way solenoid valve. Use this model with standard fractionation in order to fully attain the FRC-10A's functionality. * A "fraction-collector head" (i.e. without a valve) continuously directs the eluate to the fraction side without using a solenoid valve. It is used for micro-volume fractionation.

Specifications
Drive system

(FRC-10A)
FRC-10A (228-45070-xx) Arm-movement X-Y system 16 to 144 (depending on the type of rack used) Solenoid valve (fraction collector head with valve) or direct through nozzle (fraction collector head) 150 mL / min Basic mode (using initial parameters), and Time-Program mode (14 different functions available) Possible with sample cooler L (228-45064-xx) 4 C to 35 C 260(W) x 280(H) x 420(D)mm, 15kg AC110 V, 230V, 100 VA, 50 / 60 Hz
29

Maximum number of fractions Collection method Maximum flow rate Fraction modes Cooling function Ambient temperature range Dimensions, weight Power requirements

Pr omi nence C o m p a t i b l e U n i t s

RF-10AXL / 10AXL Super

[ Spectrofluorometric Detector ]

S u p e rior Sensit ivit y and Reliabilit y

The RF-10A XL offers excellent sensitivity, wavelength accuracy and reproducibility as well as comprehensive GLP/GMP support functions to further increase the reliability of analysis data. The wavelength scanning function determines the optimum detection wavelengths for target constituents, and highly sensitive, efficient, and simultaneous analysis of multiple constituents is performed using the wavelength programming function.
Riboflavin 10fmol Ex=450nm Em=520nm

A Step Ahead with High Sensitivity and Reliability

Using an optical system based on an improved light-source / mirror light-source compensation mechanism makes it possible to achieve a very high water-Raman signal-tonoise ratio (300 or higher). This greatly improves the precision attained in the quantitative analysis of trace constituents. More precise wavelength correction helps achieve high wavelength accuracy ( 2nm) and reproducibility ( 0.2nm). These features further increase the reliability of analysis data.

min 10

100 (%) Standard spectrofluorometric detector Change in area when room 50 temperature changes from 20C to 25C: -13.5% 0 0 100 (%) 1 2 3

At 20C At 20C

Significant Reduction of Changes in Fluorescence Intensity Affected by Room-Temperature Fluctuation (RF-10AXL Super)
The RF-10A XL Super employs a temperature-controlled cell unit to maintain a constant temperature of the liquid in the flow cell even if the room temperature changes. This greatly reduces the size of changes in the fluorescence intensity caused by fluctuations in room temperature due, for example, to temperature quenching, and makes it possible to obtain stable analysis data.

4 min

At 20C and 25C RF-10AXL Super Change in area when room temperature changes from 20C to 25C: -2.2%

50

4 min

Specifications (RF-10A XL / 10A XL Super)

RF-10AXL (228-45096-xx) Light source Spectrometer Wavelength range Bandwidth Wavelength accuracy Wavelength reproducibility Detection sensitivity Cell (volume, pressure, material) Wavelength scanning Programming Operating temperature range Dimensions, weight Power requirements 150-W xenon lamp Blazed, holographic, concave diffraction grating, F/2.4 200 to 650nm (option: 200 to 750nm *1 or 200 to 900nm *2) 15nm (on both excitation and emission sides) 2nm 0.2nm 300 or greater, signal-to-noise ratio for Raman line of distilled water (Excitation wavelength: 350nm; Time constant: 1.5sec.) 12L; 2MPa (approx. 20kgf/cm2) ; SUS316, silica, PTFE RF-10AXL Super (228-45097-xx)*

Specifications (Temperature Control Section)

Temperature setting range Recommended temperature setting Temperature accuracy 4C to 40C (on cooling side: down to 15C below room temperature) 25C (in order to prevent cell condensation) Within 2C Within 0.1C (constant room temperature) Within 0.3C (at room-temperature fluctuation of 3C / hour) Operating flow-rate range Safety features 0.2 to 2mL/min Overheating-prevention function, built-in temperature fuse

Temperature precision

Scanning of excitation and emission wavelengths; differential spectra possible Time programs for wavelength conditions of up to 32 steps can be created. 4C to 35C 260(W) x 205(H) x 520(D)mm, 17kg 4C to 40C (on cooling side: down to 15C below room temperature) 335(W) x 205(H) x 520(D)mm, 18kg

AC110V, 230V, 350VA, 50/60Hz

* 1 With optional R3788 photomultiplier (200-75031) * 2 With optional R928-08 photomultiplier (200-75021)

* RF-10AXL Super is not CE marked.

Application For Nonmetal (insert) For Semi-micro Cell volume 12L 2L Part number

Optional Cells

(Nonmetal Cell, Semi-micro Cell, Square-Cell Holders )

228-34197-92

228-34197-93

For Square cells

(10mm square cell)

206-64999

The nonmetal cell is made of PEEK and is effective for bio-inert systems used in life science or protein analysis applications. The semi-micro cell is also made of PEEK and minimizes the amount of dead volume in the tubing and cells in order to reduce band broadening. It is ideal for low flow rate (< 1 mL/min) LC systems. The square cell holder accommodates 10 mm square cuvettes to allow the detector to function as a stand-alone fluorescence spectrometer.

30

P r omi nence C o m p a t i b l e U n i t s

RID-10A
[ Refractive Index Detector ]

Improving Analysis Productivity in Refractive Index Detection

The RID-10A features dramatic improvements in the productivity of refractive index detection. Dual-temperature control of the optical system helps reduce the stabilization time and baseline drift after power-ON, and the adoption of three measurement modes allows all applications from high-sensitivity analysis to preparative work to be handled with a single unit.

Optics

Aluminum block

Short Warm-up Time and Improved Baseline Stability

The optical system is housed inside a dual-temperature-controlled block. The temperature of the incoming mobile phase is controlled in two stages; consequently, the stabilization time is significantly shorter than with conventional systems. This dual-temperature control also helps significantly reduce baseline drift, thus increasing the reliability of analysis data.

Heat insulators Heaters

Lens Light source Reference cell Sample cell Slit image

Slit

Broad Application Range - from Analytical to Preparative-Scale HPLC

The adoption of an original 4-partition detector element (U.S. Patent No. 5398110; Japanese Patent No. 2504356) makes it possible to handle all applications from high-sensitivity to preparative analyses with a single unit using the following three measurement modes.
A (Analytical) mode P (Preparative) mode L (Large-scale prep.) mode High sensitivity to general purpose analyses High concentration analysis, semi-preparative analysis (up to 20mL/min) Large volume preparative analysis with optional flow selection block (228-34102-91) for flow rates (up to 150mL/min)

4-partition detector element Mirror

A B

Even if concentration high

Balanced state

Measurable state

100

RI / 10 -4 RIU

10 1 0.1

4-Partitioned Detector Element

The detector element of the RID-10A is partitioned into four parts, and the parts used can be changed electrically. In A mode (for high-sensitivity analysis), the partitions are grouped left-right (combinations of A+C and B+D), and in P and L modes (for preparative analysis), the partitions are grouped top-bottom (combinations of A+B and C+D). In P and L modes, because measurement is possible regardless of the position of the element's center line, large refractive indexes for high-concentration samples can be handled.

0.01 0.001 0.001

0.01

0.1

10

100

Glycerin concentration unit/0.872gL - 1

Specifications (RID -10A)

RID-10A (228-45095-xx) Refractive index range Noise level Drift Range Response Polarity switching Zero adjustment 1 to 1.75RIU 2.5 x 10 -9 RIU max 1 x 10 -7 RIU/hour max. A mode: 0.01 x 10-6 to 500 x 10-6 RIU P and L modes: 1 x 10-6 to 5,000 x 10-6 RIU 0.05 to 10 sec, 10 steps With a switch Auto zero, auto-optical zero, fine zero Maximum operating flow rate Temperature control of cell unit Cell volume Cell pressure Operating temperature range Dimensions, weight Power requirements RID-10A (228-45095-xx) 20mL/min (150mL/min with option) 30 C to 60 C (1 C steps) 9L 2MPa (approx. 20kgf/cm2 ; cell unit) 4 C to 35 C 260 (W) x 140 (H) x 420 (D)mm, 12kg AC110V, 230V, 150VA, 50/60Hz

* Hexafluoroisopropanol (HFIP) cannot be used as the mobile phase.

Option: Pressure Relief Valve (228-33615-91)

The RID-10A incorporates various safety features. Its maximum pressure is five times that of former Shimadzu products and, as a standard feature, it incorporates a sensor that detects leakage from the cell unit. For extra safety, a pressure relief valve that prevents problems related to back-pressure irregularities is also available as an option.
31

Pr omi nence C o m p a t i b l e U n i t s

CDD-10AVP
[ Conductivity Detector ]

H a n d le s a W ide Variet y of Analysis Opt ions

The CDD-10AVP conductivity detector achieves an even higher level of sensitivity and makes it possible to perform a wide variety of analysis scenarios with a single unit. An option card enables the simultaneous 2-channel measurement of anions and cations, and a suppressor option allows expansion to a suppressor system for ultra-high sensitivity work. Organic acids can be analyzed using Shimadzu's unique post-column pH-buffered electroconductivity method.
Ions F Cl NO2 Br NO3 PO4 SO3 Concentration (g/L) 50 200 15 100 80 500 200 RSD (%) 0.46 0.23 5.41 0.71 0.54 0.63 2.30

Perform Analysis with Highest Sensitivity

The sensitivity of detectors that monitor weak electrical signals from analytes is affected by the inherent electrical noise of the detector itself. With the CDD-10A VP , electronic parts with low electrical noise are used, and the layout of the electronic components has been optimized in order to reduce noise levels, thereby attaining an extremely high level of sensitivity. Combining the CDD-10A VP with a suppressor unit makes it possible to perform ultra-high sensitivity ion analysis on the order of 0.25 g / L (detection limit: S / N=3) for Cl - .

Reproducibility in Anion Analysis

(Lower concentration limits of quantitative analysis range for 2001 edition of Water Supply Testing Methods, Japan)

Applicable to Both Suppressor and Non-Suppressor Systems (available in limited regions)

When used with a CTO-20AC, expansion to a full suppressor system can be realized by adding the suppressor option. Suppressor functions can be disabled when necessary, making it possible to switch between anion analysis using a suppressor system and cation analysis using a non-suppressed system. In addition to a single flow-line system, expansion to a dual flow-line system is also possible, allowing the creation of a variety of system configurations. For example, simultaneous analysis of anions and cations using a combination of suppressed and non-suppressed detection is possible.
Peaks
1. Phosphoric acid 2. Citric acid 4 3. Pyruvic acid 4. Malic acid 2 5. Succinic acid 6. Lactic acid 1 3 7. Formic acid 8. Acetic acid 9. Levulinic acid 10. Pyroglutamic acid 11. Iso-butyric acid 12. n-butyric acid 7

High-Sensitivity Analysis of Organic Acids

Shimadzu's post-column pH-buffered electroconductivity method (Patent No. 2017498) enables selective, high-sensitivity analysis of organic acids. Even samples that traditionally require time-consuming pretreatment to handle unwanted constituents can be analyzed after simple pretreatment procedures such as dilution and filtration. The level of reliability attained in quantitative analysis is much higher than that attained conventionally with a low-wavelength UV method or a simple conductivity method. Superior linearity enables batch analysis in cases where constituent concentrations differ greatly and, consequently, helps reduce analysis time.

5 8 6 9 10 11 12

10

20

30

min

Specifications (CDD-10A VP)

CDD-10A VP ( 228-45054-xx) Temperature coefficient Cell volume Cell constant Material used in parts making contact with liquid Maximum operating pressure Response Zero adjustment Operating temperature range Dimensions, weight Power requirements
32

25nScm -1 / C (background: 285 Scm -1 ; cell temperature: 43 C) 0.25 L 25 Scm -1 PEEK, SUS316 2.9MPa (30kgf/cm 2 ) 0.05 to 10 sec., 10 steps Autozero, baseline shift 4 C to 35 C 260 (W) x 140 (H) x 420 (D)mm, 6.0kg AC110VA, 230V, 250VA, 50/60Hz

P r omi nence C o m p a t i b l e U n i t s

ELSD-LT@
[ Evaporative Light-Scattering Detector ]

H ig h ly Versat ile Det ect ion M et hod

Not all compounds have a chromophore or other such structural property that allows the use of an absorbance detector. Refractive Index Detection (RID) is one option but it suffers from the inability to run gradient analysis. Evaporative Light Scattering Detection (ELSD) is a perfect alternative to RID as it is more rugged, quicker to stabilize, and gradient compatible. ELSD is ideal for applications like testing the purity of compounds, measuring the molecular weight distribution of synthetic polymers, and analyzing natural substances. Detects Most Compounds
With the exception of some highly volatile compounds, the ELSD-LT2 is able to detect almost any compound. Unlike traditional absorbance detectors (UV-Vis, PDA, etc.), sensitivity is not dependent on the physical or structural properties of the compound, but rather the absolute quantity of the solute passing through the detector cell. Therefore, it is especially useful for detecting unknown or breakdown compounds and/or validating purity of a target compound. By this mechanism of detection the ELSD-LT2 is truly a universal detector. Note:Due to the evaporative nature of this detector, it must only be used in an area with proper exhaust ventilation.

Analytical Conditions Column: Shim-pack VP-ODS (4.6mm i.d. x 150mm) Mobile Phase: Water/Methanol Gradient (60/40-40/60, 2%/min) Flow Rate: 1mL/min Temperature: 40 deg. C Sample: PEG-1000

10

15min

Chromatogram of Non-Chromophoric Compound

Photomultiplier

Light Source

High Sensitivity by Low Temperature Evaporation

The ELSD-LT2 detector uses a unique nebulizer and drift tube design to achieve stable and low-temperature evaporation of mobile phases, making it possible to analyze semivolatile and / or thermally unstable compounds. High-sensitivity detection is achieved by focusing the sample at the detection point with assist gas flow. The ELSD-LT2 offers high sensitivity with this low-temperature evaporation technology and superb detection technology. A smaller volume nebulizer and drift tube further improve sensitivity.

Assist Gas

Assist Gas

Assist Gas Functions

mV 350

300

1.Methylparaben 100ng 2.Ethylparaben 100 ng 3.Propylparaben 100ng 4.Butylparaben 100ng

(S/N=101) (S/N=146) (S/N=276) (S/N=337)

250

200

Automated Functions
2

150

Auto-Powerdown functions for the LED light source and nebulizer gas reduce operating costs. The self-cleaning design makes maintenance of the drift tube easier.

100

50

-50 0.00

Example of analyzing 4 semi-volatile alkyl parabens, considered difficult to analyze with conventional ELSD detectors.
0.25 0.50 0.75 1.00 1.25 1.50 min

Specifications
Measurement Method Light Source Detection Temperature Setting Range Nebulizer Gas Gas Flow Rate Gas Pressure

(ELSD-LT @ )
ELSD-LT2 (228-45115-xx) Light Scattering LED Photomultiplier Tube Ambient to 80C Nitrogen (N2) or Air (see Note 1) Max. 3.0 L/min Max. 450 kPa Mobile Phase Flow Rate Analog Output Operating Temperature Range Operating Humidity Range Power Supply Size Weight ELSD-LT2 (228-45115-xx) 0.2mL/min - 2.5mL/min (see Note 2) 0V - 1V 5C - 40C <80% (5C - 31C), <50% (31C - 40C) AC 115V, 230V, 150VA, 50/60Hz W250 x D550 x H450mm 20kg

Note 1: Requires gas supply source, such as a gas line, nitrogen generator, or air compressor. Note 2: 0.04 mL/min to 1.2 mL/min range when using a low-flow nebulizer.
33

O pt i ons

Optional Accessories
FCV Series Flow-Line Selection Valves Reservoir Selection Valves

FCV-11AL FCV-11ALS

(228-45048-91) (228-45049-91)
1 2 3 To solvent delivery units 3-way solenoid valve FCV-11AL (The FCV-11ALS has only one solenoid valve.)

These solenoid valve units can automatically switch between two solvents (e.g., mobile phase and column rinse solvent) plumbed to one solvent delivery unit. The FCV-11AL can handle the automatic selection of solvents for up to three solvent delivery units whereas the FCV-11ALS is used for one unit.

High-Pressure Flow-Line Selection Valves

FCV-12AH (228-45013-91) FCV-12AHi (228-45013-94)

Injector

Column 1

Detector FCV-12AH

These flow-line selection valves incorporate 6-por t, 2-position, high-pressure valves. They can be used for automatic column selection and automatic pretreatment. * The liquid contact par ts of the FCV-12AHi have bio-iner t specifications.

Column 2

Reservoir Selection Valve

To solvent delivery unit

FCV-13AL (228-45016-91)
This unit performs automatic solvent selection and incorporates a 7-port, 6-position valve. It can perform the switching of up to six solvents for a stepwise gradient.

FCV-13AL

Column Switching Valves

FCV-14AH (228-45014-91) FCV-14AHi (228-45014-92)

This unit performs automatic column selection and incorporates a 7-port, 6-position, high-pressure valve. It can be used for automatic multi-column switching. (Two units used.) * The liquid contact parts of the FCV-14AHi have bio-inert specifications.
Injector
1 6 4 5 2 3

Column 1
2 3 4 5 4 5 3 2 1 6

Detector

FCV-14AH

FCV-14AH

34

Reservoir Selection Valve

FCV-7AL (228-45077-91)
This device can switch between two solvents using a solenoid valve. It incorporates a pump that can automatically rinse the rear side of the solvent delivery unit's plunger seals. It can be controlled from the LC-6AD or from a system controller or workstation connected via the LC-6AD.

Reservoir Selection Valve

FCV-130AL (228-45078-91)
This device can switch between two solvents using a solenoid valve. It incorporates a pump that can automatically rinse the rear side of the solvent delivery unit's plunger seals. It can be controlled from the LC-8A or from a system controller or workstation connected via the LC-8A.

FCV Series Specifications

FCV-11AL Valve type Solvent pH usage range Maximum pressure (kgf /cm2) Valve switching time Control Dimensions Weight

(FCV-11AL / 11ALS /12AH /13AL /14AH)

FCV-11ALS One 3-way solenoid valve FCV-12AH One 6-port, 2-position valve 1 to 10 350 1 to 14 5 SCL-10AVP *2 110(W) x 250(D) x 110(H)mm 2.0kg 4.0kg FCV-13AL FCV-14AH

Three 3-way solenoid valve

One 7-port, 6-position valve 1 to 10 350 0.3 sec.

1 to 14 0.5 0.02 sec LC-VP solvent delivery unit or SCL-10AVP *1

* 1 An Option Box VP or a Sub-controller VP is required for control of the FCV-12AH /13AL /14AH. This does not apply to the FCV-12AH /14AH when it is connected to the CTO-20A/20AC. Two FCV-12AH units and a total of two FCV-13AL or FCV-14AH units can be controlled from the SCL-10A VP . * 2 When using FCV-11AL /11ALS/15AL units for solvent selection, only one of these units can be controlled from the SCL-10A VP or a solvent delivery unit. The SCL-10A VP and Option Box VP or Sub-controller VP is required to use two of these units simultaneously.

Column Oven Compact and affordable

Specifications
Type Temperature setting range Temperature control precision Temperature control range

(CTO-10AS VP )
CTO-10ASVP Biock heating 4 to 80C sept 0.1C (Ambient temp,-15)C to Room Ambient temp +60C Pre-heating, 10Lx2 channel 25cm(2 columns max.) a) Temperature limit device using maximum temperature setting b) Thermal Fuse c) Solvent leak sensor Temperature setting changes Oven ON/OFF 320 steps, 0.1 - 999.9 min 130(W) x 420(D) x 415(H) mm 12kg 4 to 35C AC 110V, 230V, 120VA,50/60Hz
35

CTO-10AS VP (228-45059-xx)
The CTO-10AS VP is a space-saving, compact and affordable oven with a block heating mechanism. The CTO-10AS VP controls temperature from ambient -15 C, and accommodates two manual injectors.

Temperature control mechanism Columns accommodatable Safety features

Time program Dimensions Weight Ambient Temperature range Power requirements

O pt i ons

O pt i o n s fo r S o lv e n t D eliv e r y U nit s Automatic Rinsing Kit

20AD Automatic Rinsing Kit (228-45567-91) 20AT Automatic Rinsing Kit (228-45568-91) 20AB Automatic Rinsing Kit (228-18803-92) These optional kits are used to continuously, automatically rinse the backs of the plunger seals and plunger units. They wash away the salt that is deposited on the surfaces of the seals and plungers when buffer solution is used as the mobile phase, thereby helping to prolong the service life of these parts. There are kits for use with the LC-20AD, the LC-20AT, and the LC-20AB.

* The automatic rinse kit for the LC-20AB is shown in the photograph.

Mixer
Mixer 0.5-2.6mL HP (228-45093-93) Mixer 100 L HP (228-35830-93) 20A Bio-inert Mixer (228-45093-92) 8A Preparative Mixer (228-20600-91) 6AD Preparative Mixer (228-20738-92) These gradient mixers offer superior mixing performance. Mixing volumes of 0.5mL, 1.7mL, and 2.6mL can be selected for the Mixer 0.5-2.6mL HP. The mixing volume for the Mixer 100 L HP is 100 L. The 20A bio-inert mixer incorporates PEEK resin and ceramic for use with bio-inert systems, and two mixing volumes can be selected. There are also mixers for preparative applications. * The Mixer 0.5-2.6mL HP is shown in the photograph.

Helium Degasser

DGU-10B (228-45067-93)
This degasser purges dissolved air from the mobile phase and prevents phenomena such as bubble formation, baseline noise, and drift. The DGU-10B can degas up to four mobile phase solutions with helium gas. It is turned ON/OFF from the solvent delivery unit or system controller.

O pt i o n s fo r C h emic a l R ea c tio n Unit s Chemical Reaction Chamber

CRB-6A (228-45065-XX)
This air circulation-type reaction chamber is used for post-column derivatization. Temperature control range: Between 15 C above room temperature and 150 C Temperature control precision: 0.1 C (100V operation only)

Reagent Delivery Pump

PRR-2A (228-07849)
This peristaltic pump delivers reagent and is used for post-column derivatization. It can deliver up to five liquids. (100V operation only)

O pt i o n s fo r S amp le In jec tio n U nit s Sample Injectors

Rheodyne Rheodyne Rheodyne Rheodyne Rheodyne 7725 (228-32210-91) For general analysis 7725i (228-32210-93) For general analysis 8125 (228-23200-91) For semi-micro systems 9725 (228-32650-91) For bio-inert LC systems 9725i (228-32650-93) For bio-inert LC systems

Optional Loops
Volume 100 L 200 L 500 L Material SUS PEEK SUS PEEK SUS PEEK SUS PEEK Part Number 228-32211-16 228-32651-16 228-32211-17 228-32651-17 228-32211-18 228-32651-18 228-32211-19 228-32651-18

* The Rheodyne 7725i/9725i incorporates a position-sensing switch.

36

1 mL

Va l v e O p t i o n s Option Box VP (228-45060-xx) Sub-controller VP (228-35308-xx)

Option Box VP can house up to two FCV-11AL (S)/12AH/13AL/14AH units. One FCV-11AL or FCV11ALS unit, up to two FCV-12AH units, up to two FCV-13AL/14AH units, and one DGU-10B unit can be controlled from the CBM-20A or SCL-10AVP via Option Box VP. Sub-controller VP has exactly the same control functions as Option Box VP but has no housing capability. * Option Box VP is shown in the photograph.

Solvent Recycle Valve Kit (228-45080-91)

Using a solvent recycle valve kit during isocratic analysis allows column eluent to return to the reservoir bottle when no peaks are detected according to the set threshold level. This helps reduce consumption of the mobile phase, especially at higher flow rates.

Manual Recycle Valve (228-20401-92)

This manual switching valve is used to perform recycling operations with preparative systems.

Manual Column Switching Valve (228-13000-95)

This manual switching valve is used to switch between preparative columns, or between a preparative column and an analytical column, in an analytical to preparative scaleup system.

O th e r O p t i o n s Reservoir Tray (228-45041-91)

The sturdy plastic tray will hold up to seven 1-liter reservoir bottles. Access behind the front panel allows for neat routing of reservoir tubing.

8A Column Holder (228-45079-91)

This holder supports the mounting of two columns with inner diameters in the range of 20 to 50mm, one analytical column, four manual selection valves of various types, and an 8A preparative mixer or an 8A analytical mixer.

37

LCMS-2020
MS a Mass Spectrometer is the ultimate detector for a chromatograph. Using the LCMS-2020 as a detector for HPLC significantly enhances the application range. The LCMS-2020 is compatible with ultra-fast LC(UFLC) analysis as well as conventional HPLC.

UFscanning

15,000 u/sec fast scanning speed

Controls the voltage applied to the Quadrupole according to the scan speed and m/z. Adopting this new technology (patent pending) maintains resolution and achieves high ion transmittance even at high scanning speeds.

UFswitching

Rapid 15-millisecond positive/negative ionization switching

To detect both positive and negative ions, analysis is performed while switching between the positive and negative ionization modes. The LCMS-2020 adopts a high-voltage power supply featuring novel technology (patent pending) to achieve an ultra-fast polarity switching time of just 15 ms.

UFsensitivity

Superior sensitivity from UFLC

The newly developed Qarray ion optical system achieves superior sensitivity, reproducibility, and linearity.
38

U Fs c a n n i n g & U F switch in g
UFLC/MS Measurement
UFscanning and UFswitching are critical for ultra-fast analysis. For example, in ultra-fast analysis where 6 compounds may elute within 1 minute, ultra-fast (MS measurement) detection is also required. The UFswitching and UFscanning functions are what make possible such ultra-fast MS measurement.
15 milliseconds polarity switching Positive ion measurement 15,000 u/sec Negative ion measurement 15,000 u/sec 15 milliseconds polarity switching Positive ion measurement 15,000 u/sec

U Fs e n s i t i v i t y
Ultra-fast analysis with excellent sensitivity
Newly developed ion optical system and new Qarray provide excellent sensitivity, repeatability and linearity.
Calibration curve

H a rdw a re fe a tu re s th a t p ow erfu lly suppor t 3 t ypes of UF f unct ionalit y

Toughness against dirty samples
In order to check the toughness of the LCMS2020 against dirty samples, plasma samples simply precipitated with only acetonitrile were injected 2,500 times over 10 days (1L volume per injection). Excellent reproducibility of peak area was demonstrated and its c.v. was 2.26%.

Easy Maintenance
The DL capillary (desolvation line), which transfers the sample into the vacuum chamber from the ion source, can be installed and removed without breaking the vacuum, greatly speeding maintenance operations.

Analysis time for approx. 10 days

Creating Fragment Ions by In-source CID

In-source CID is effective for confirming the molecular weight of synthetic compounds and for the quantification of impurities. Using in-source CID (collision-induced dissociation) allows the generation of fragment ions. This example shows the composition of impurities in erythromycin estimated from fragment ions generated by in-source CID. The multi-sequence mode permits several other methods within a single analysis, such as CID, positive/negative ion switching modes, and SCAN/SIM modes. Precisely setting the parameters reduces the risk of erroneous evaluations and enhances the reliability of analysis results.
DL=0V Qarray DC=0V
(x1,000,000) 2.0

TIC chromatogram
1.5

DL=0V Qarray DC=0V

Erythromycin (major component)
5.0 Inten.(x100,000)

DL= 0 V Qarray DC= 70 V

Inten.(x100,000) 3.0

1.0

Erythromycin (major component)

734
2.0

158 576 116 558 734

CID
0.5 2.5 1.0

0.0 10 20 30 0.0 250 500 m/z 0.0 250 500 m/z

(x1,00,000)

Magnified view
3
3.0

Inten.(x1,000)

Inten.(x1,000) 1.5

TIC chromatogram
2.0

Peak 5

732
1.0

574 732

Erythromycin (major component)

2 4 5

2.0

CID
0.5

1.0

1.0

556
0.0 250 500 m/z 0.0 250 500 m/z

0.0 10.0 15.0 20.0 25.0

39

JQA-0376

Founded in 1875, Shimadzu Corporation, a leader in the development of advanced technologies, has a distinguished history of innovation built on the foundation of contributing to society through science and technology. We maintain a global network of sales, service, technical support and applications centers on six continents, and have established long-term relationships with a host of highly trained distributors located in over 100 countries. For information about Shimadzu, and to contact your local office, please visit our Web site at

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