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    Photosynthesis: Electron Flow and The Hill Reaction: Pictures

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    Bio Sciences
    This document summarizes an experiment on photosynthesis and the Hill reaction. It describes how Robert Hill demonstrated that water, not carbon dioxide, is the source of electrons in the light reaction of photosynthesis. The experiment will examine how chemicals that inhibit different parts of the photosynthetic light reaction affect the reaction rate. Specifically, ammonia blocks ATP production while DCMU stops both ATP and NADPH synthesis. The experiment will also assess how different light intensities affect the rate of electron transport by measuring changes in absorbance of DCIP, an electron acceptor.

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    Photosynthesis: Electron Flow and The Hill Reaction: Pictures

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    Bio Sciences
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    This document summarizes an experiment on photosynthesis and the Hill reaction. It describes how Robert Hill demonstrated that water, not carbon dioxide, is the source of electrons in the light reaction of photosynthesis. The experiment will examine how chemicals that inhibit different parts of the photosynthetic light reaction affect the reaction rate. Specifically, ammonia blocks ATP production while DCMU stops both ATP and NADPH synthesis. The experiment will also assess how different light intensities affect the rate of electron transport by measuring changes in absorbance of DCIP, an electron acceptor.

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    Photosynthesis

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    This document summarizes an experiment on photosynthesis and the Hill reaction. It describes how Robert Hill demonstrated that water, not carbon dioxide, is the source of electrons in the light reaction of photosynthesis. The experiment will examine how chemicals that inhibit different parts of the photosynthetic light reaction affect the reaction rate. Specifically, ammonia blocks ATP production while DCMU stops both ATP and NADPH synthesis. The experiment will also assess how different light intensities affect the rate of electron transport by measuring changes in absorbance of DCIP, an electron acceptor.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    39 views3 pages

    Photosynthesis: Electron Flow and The Hill Reaction: Pictures

    Uploaded by

    Bio Sciences
    This document summarizes an experiment on photosynthesis and the Hill reaction. It describes how Robert Hill demonstrated that water, not carbon dioxide, is the source of electrons in the light reaction of photosynthesis. The experiment will examine how chemicals that inhibit different parts of the photosynthetic light reaction affect the reaction rate. Specifically, ammonia blocks ATP production while DCMU stops both ATP and NADPH synthesis. The experiment will also assess how different light intensities affect the rate of electron transport by measuring changes in absorbance of DCIP, an electron acceptor.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
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    Photosynthesis: Electron flow and the Hill reaction

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    Background Robert Hill demonstrated that during photosynthesis water is the source of electrons in the light reaction and not carbon dioxide by using an experimental design similar to the one you will be using today. The conclusions drawn from this experiment can be summarized in the equation below:

    In vivo the electron acceptor is NADP+, however, in vitro certain dyes such as 2,6dichlorophenolindophenol (DCIP) can act as the electron acceptor. An equation to describe this chemically is:

    DCIP is blue when it is oxidized but is colorless when is reduced (has accepted electrons). This change can be measured in a spectrophotometer as a change in the absorbance at the 600 nm wavelength of light.

    Two main points will be addressed in this experiment: 1. Chemicals which act to inhibit different points of the photosynthetic light reaction will be administered and their effect on the reaction rate well be addressed.

    Ammonia blocks the pathways ability to manufacture ATP, by short circuiting the hydrogen ion gradient, but does not block the production of NADPH + H+. 3-(3,4-dichloro-phenyl)-1,1-dimethylurea (DCMU) blocks the electron transport chain early in the pathway stopping both ATP and NADPH synthesis.

    2. The chloroplasts will be exposed to different intensities of light and the rate of electron transport will be assessed.

    Experimental Protocol:
    1. There will be 4 people per lab group

    2. Two people will work up the chloroplasts while the other two will set up the 8 reaction cuvettes described in the table on p. 57. DO NOT ADD THE CHLOROPLASTS UNTIL THE REACTION IS RUN!!

    3. Turn on the spectronic 20 when you start to isolate the chloroplasts from the spinach leaves. 4. Be sure to keep everything chilled on ice, as described in the lab manual, when working with the chloroplasts. 5. All 4 group members will be involved in doing the reactions described in part B.

    Each reaction is done in a single cuvette Do only one reaction at a time Add the chloroplasts to the reaction cuvette just prior to each reaction There will be 9 absorbance readings for each reaction (except for tubes 1 and 2) Take the spectrophotometer readings as quickly as possible Change the RT water in the beaker between each reaction Zero the spectrophotometer between each reaction

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