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    Mumtaz

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    Saqib Mahmood Bhutta
    The document summarizes the results of an HCV Real Time PCR test performed on a blood sample from a 30-year-old female patient named Mumtaz Bibi. The test detected 8,61,460 IU/ml of HCV RNA in the patient's sample. Real Time PCR is used to amplify and quantify specific regions of the HCV genome using fluorescent dyes and probes. The test has an analytical sensitivity of 26.3 IU/ml and is approved for human in vitro diagnosis.

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    Mumtaz

    Uploaded by

    Saqib Mahmood Bhutta
    10 views1 page
    The document summarizes the results of an HCV Real Time PCR test performed on a blood sample from a 30-year-old female patient named Mumtaz Bibi. The test detected 8,61,460 IU/ml of HCV RNA in the patient's sample. Real Time PCR is used to amplify and quantify specific regions of the HCV genome using fluorescent dyes and probes. The test has an analytical sensitivity of 26.3 IU/ml and is approved for human in vitro diagnosis.

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    The document summarizes the results of an HCV Real Time PCR test performed on a blood sample from a 30-year-old female patient named Mumtaz Bibi. The test detected 8,61,460 IU/ml of HCV RNA in the patient's sample. Real Time PCR is used to amplify and quantify specific regions of the HCV genome using fluorescent dyes and probes. The test has an analytical sensitivity of 26.3 IU/ml and is approved for human in vitro diagnosis.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    10 views1 page

    Mumtaz

    Uploaded by

    Saqib Mahmood Bhutta
    The document summarizes the results of an HCV Real Time PCR test performed on a blood sample from a 30-year-old female patient named Mumtaz Bibi. The test detected 8,61,460 IU/ml of HCV RNA in the patient's sample. Real Time PCR is used to amplify and quantify specific regions of the HCV genome using fluorescent dyes and probes. The test has an analytical sensitivity of 26.3 IU/ml and is approved for human in vitro diagnosis.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    of 1

    Patients Name: Lab Reg.

    No: Referred by: Test Requested:

    Mumtaz Bibi 2037 Self HCV Real Time PCR Test

    Age / Sex: Sample Date: Report Date: Specimen:

    30 Years / Female 27-09-2011 30-09-2011 Blood

    HCV Real Time PCR Quantitation:


    Test Name HCV RNA Real Time PCR Viral Load of HCV RNA Patient Result Detected 8,61,460 IU/ml

    HCV RNA isolated from blood sample of patient, using QIAamp Viral RNA Mini Kit. HCV RNA if present in the patient sample is amplified using Qiagen HCV RTPCR Kit on the Real Time PCR system Rotor Gene Q. HCV diagnosis by the polymerase chain reaction (PCR) is based on the amplification of specific regions of the pathogen genome. In Real-time PCR the amplified product is detected via fluorescent dyes. These are usually linked to oligonucleotide probes which bind specifically to the amplified product. Monitoring the fluorescence intensities during the PCR run (i.e. in real-time) allows the detection and quantitation of the accumulating product without having to re-open the reaction tubes after the PCR run (Mackay, 2004). Real Time PCR is a precision science that demands High thermal and optical uniformity, short equilibration times and fast ramping rates of Real Time PCR system QIAGENs Real Time PCR system Rotor-Gene Q combines multiple optimized design features to provide the outstanding performance and reliable results for In-vitro Human Diagnosis. HCV RTPCR Kit contains reagents and enzymes for the specific amplification and detection of 240 bp region of HCV genome on Rotor- Gene Q. In addition, the Kit contains a second heterologous amplification system to identify possible PCR inhibition. This is detected as an Internal Control (IC) in 2nd fluorescence channel. WHO reference standards are used for quantitation of HCV RNA. The Analytical Sensitivity of this test is 26.3 IU/ml. These Kits are CE Marked (CE # 0197) and approved for Human In-Vitro Diagnosis. The result can not be compared with any other laboratory as every lab has its own procedures and quality control. Note: This report is not valid for Court.

    Electronically verified report does not require signature.

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