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    And CA10 Calli

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    hrishikesh08
    After 15 days of cultivation on media A4 and A10, embryogenic/organogenic calli showed no visible differences between the two conditions. However, over time differences began to emerge, with calli on A10 medium developing yellow regions and shoot primordia earlier than those on A4 medium. By 75 days, calli on A10 medium showed more differentiated protocorm-like bodies and root primordia compared to calli on A4 medium.

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    And CA10 Calli

    Uploaded by

    hrishikesh08
    7 views1 page
    After 15 days of cultivation on media A4 and A10, embryogenic/organogenic calli showed no visible differences between the two conditions. However, over time differences began to emerge, with calli on A10 medium developing yellow regions and shoot primordia earlier than those on A4 medium. By 75 days, calli on A10 medium showed more differentiated protocorm-like bodies and root primordia compared to calli on A4 medium.

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    1471-2229-10-82-s6

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    After 15 days of cultivation on media A4 and A10, embryogenic/organogenic calli showed no visible differences between the two conditions. However, over time differences began to emerge, with calli on A10 medium developing yellow regions and shoot primordia earlier than those on A4 medium. By 75 days, calli on A10 medium showed more differentiated protocorm-like bodies and root primordia compared to calli on A4 medium.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    7 views1 page

    And CA10 Calli

    Uploaded by

    hrishikesh08
    After 15 days of cultivation on media A4 and A10, embryogenic/organogenic calli showed no visible differences between the two conditions. However, over time differences began to emerge, with calli on A10 medium developing yellow regions and shoot primordia earlier than those on A4 medium. By 75 days, calli on A10 medium showed more differentiated protocorm-like bodies and root primordia compared to calli on A4 medium.

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    Additional file 6. Appearance of embryogenic/organogenic calli cultivated onto A4 medium and A10 medium.

    After 15 days, there were no visible differences between the CA4 and CA10 calli.
    Embryogenic/organogenic calli Observation times 15 days 20 days on A4 medium (CA4) Calli Calli* on A10 medium (CA10) Calli Calli with yellow regions Calli with pale green or green shoot primordia : early stage of protocorm-like body (PLB) development Calli with yellow-green PLBs Calli with yellow-green regions 60 days and green-differentiated PLBs wearing leaf sheath Calli with yellow-green PLBs Calli with yellow-green regions 75 days and green-differentiated PLBs; root primodia emerging Callus with green and deep green PLBs; regeneration of one or two roots Embryogenic/organogenic calli

    Calli* with yellow and pale 30 days green regions

    Calli with pale green and poorly-differentiated structures; 90 days some regions started to turn brown Calli with pale green and 120 days poorly-differentiated structures; necrosis was observed

    Calli with very differentiated PLBs, PLB-derived plantlets and well-developed roots

    * After 20 days, 20 - 30% of organogenic calli exhibited white nodular compact structures.

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