Potassium Colorimetric BXC0132

BXC0132A
2x30ml
STORE AT ROOM TEMPERATURE TO 25C
FOR IN-VITRO DIAGNOSTIC USE ONLY
ISO 13485 accredited company
POTASSIUM
Manual Procedure:
Wavelength
Temperature
578 nm,
20-25oC
Hg 578 nm
COLORIMETRIC
Kit Contents:
A.
NaOH
B.
Sodium Tetraphenylboron
C. Precipitating Reagent
D.
Potassium Standard
BXC0132A
1 x 30 ml
1 x 30 ml
1 x 60 ml
1 x 5 ml
Test Principle:
Sodium tetraphenylboron reacts with potassium ions in a protein free
alkaline medium to produce a turbid suspension of potassium
tetraphenylboron. The depth of turbidity is proportional to the
potassium concentration.
Clinical Significance:
Increased levels of potassium in serum can indicate renal failure,
severe bleeding or tissue damage.
Decreased levels are a common electrolyte abnormality and can be
due to renal wastage, deficient intake or taking of diuretic
medication.
Reagent Concentration:
NaOH
Sodium Tetraphenylboron
Precipitating Reagent
Standard
2.00 mol/l
0.20 mol/l
0.30 mol/l
0.5 mmol/l
Reagent Handling and Preparation:

Reagents are supplied ready to use and will be stable to the expiry
date quoted when stored in the recommended way.
Working Reagent:
To prepare a working reagent for the test, mix equal quantities of
Sodium tetraphenylboron and sodium hydroxide. The mix will be
stable for up to 30 days at room temperature 15-25oC or 60 days at +2
-+8oC.
The standard concentration for the purpose of the calculation to
compensate for the dilution effect of the precipitating step should be
taken as 5.00 mmol/l.
Sample:
Serum or Lithium-heparin plasma
Specimen:
Separate serum or plasma from the red blood cells within 1 hour after
collection.
Cuvette
1 cm light
path
BXC0132 POTASSIUM Page 1 of 1
Measurement
Against
reagent blank
Mix 50 l of sample with 500 l of the precipitating reagent and

centrifuge at a minimum of 4000 rpm for 5-10 minutes. Remove the
supernatant and prepare test-tubes as follows:
Standard
Sample
Working Reagent
1.00 ml
1.00 ml
Standard
100 l
--Supernatant
--100 l
The standard and the clear supernatant must be added to the
middle of the working reagent surface to produce a homogeneous
turbidity.
Mix carefully and leave for 5 minutes at room temperature.
Measure the absorbance of the standard and samples against the
reagent blank.
Calculation:
Potassium Conc = Abs sample
x 5.0 mmol/l
Abs standard
Linearity:
Up to 10 mmol/l. Samples above this should be diluted with 0.9% NaCl
and reassayed, multiplying the result by the dilution factor.
Normal Values:
Serum
Plasma
3.6-5.5 mmol/l
Male: 4.0-4.8 mmol/l

Female: 3.4-4.4 mmol/l
Fortress Normal Bovine Assayed Control Cat No BCX0313A (10x5ml)

Fortress Elevated Bovine Assayed Control Cat No BXC0313B (10x5ml)
Fortress Normal Human Assayed Control Cat No BXC03112A (10x5ml)
Fortress Elevated Human Assayed Control Cat No BXC0312B (10x5ml)
Fortress QC materials of human source have been tested at donor
level for HbsAg Antigen, HIV1&2 antibodies and HCV antibody and
found to be negative.
However no test can offer complete
assurance to the absence of infectious diseases so all material should
be handled and disposed of as if it is potentially infectious.
Some common reasons for incorrect results can be:
1.
Wavelength used for the determination
2.
Light source
3.
Temperature
4.
Cleanliness, e.g of cuvettes used in
measurements
5.
Bacterial contamination of reagent
6.
Reagent expiry
7.
Calibration frequency
Health & Safety:
This kit is designed for use by suitably qualified laboratory personnel
only. Exercise the normal precautions required for the handling of
laboratory reagents. Do not ingest the material. Dispose of material
according to local guidelines.
References:
1.
Tietz, NW. Clinical Guide to laboratory tests
3rd Edn. 1995
2.
Henry, JB. Clinical Diagnosis & Management by Laboratory
Methods 20th Edn 2001.
These values are supplied as a guideline only.

It is recommended that a laboratory establishes its own reference range,
which may be dependant on age, diet geographical location and

sample type.
Interference:
Haemolysis will interfere with the assay due to the high potassium
content of red blood cells; therefore, samples should be separated
from the clot as soon as possible. Avoid contamination by detergents.
To reduce contamination, Disposable plasticware are recommended.
Quality Control:
It is recommended that a laboratory uses normal and abnormal
reference control sera to verify the performance of the procedure,
both performance of the reagent and any instrumentation employed
in the determination. Results obtained should fall within the specified
ranges.
Fortress Diagnostics Limited, Unit 2C Antrim Technology Park, Antrim BT41 1QS (United Kingdom)
TEL: +44 (0) 2894 487676 FAX: +44 (0) 2894 469933 www.Fortressdiagnostics.com
For In Vitro Diagnostics Use Only

Lot Number
Catalogue Number
Storage Temperature
Expiry Date (Year / Month)
Warning, Read Enclosed Documents
Instructions For Use
Manufactured By
REVISED OCT/07

Potassium Colorimetric BXC0132

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BXC0132A

ISO 13485 accredited company

Reagent Handling and Preparation:

BXC0132 POTASSIUM Page 1 of 1

Mix 50 l of sample with 500 l of the precipitating reagent and

Male: 4.0-4.8 mmol/l

Fortress Normal Bovine Assayed Control Cat No BCX0313A (10x5ml)

These values are supplied as a guideline only.

which may be dependant on age, diet geographical location and

For In Vitro Diagnostics Use Only

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