Microbial fermentation is commonly used to produce lactic acid and offers advantages over chemical synthesis. Lactic acid bacteria convert glucose to lactic acid under anaerobic conditions, producing two lactic acid molecules from each glucose molecule. The typical fermentation process for producing lactic acid from cellulosic biomass involves four steps - collecting and pretreating biomass, enzymatic hydrolysis to break down polysaccharides, fermenting sugars into lactic acid using bacteria, and separating and purifying the lactic acid.
Microbial fermentation is commonly used to produce lactic acid and offers advantages over chemical synthesis. Lactic acid bacteria convert glucose to lactic acid under anaerobic conditions, producing two lactic acid molecules from each glucose molecule. The typical fermentation process for producing lactic acid from cellulosic biomass involves four steps - collecting and pretreating biomass, enzymatic hydrolysis to break down polysaccharides, fermenting sugars into lactic acid using bacteria, and separating and purifying the lactic acid.
Microbial fermentation is commonly used to produce lactic acid and offers advantages over chemical synthesis. Lactic acid bacteria convert glucose to lactic acid under anaerobic conditions, producing two lactic acid molecules from each glucose molecule. The typical fermentation process for producing lactic acid from cellulosic biomass involves four steps - collecting and pretreating biomass, enzymatic hydrolysis to break down polysaccharides, fermenting sugars into lactic acid using bacteria, and separating and purifying the lactic acid.
Microbial fermentation is commonly used to produce lactic acid and offers advantages over chemical synthesis. Lactic acid bacteria convert glucose to lactic acid under anaerobic conditions, producing two lactic acid molecules from each glucose molecule. The typical fermentation process for producing lactic acid from cellulosic biomass involves four steps - collecting and pretreating biomass, enzymatic hydrolysis to break down polysaccharides, fermenting sugars into lactic acid using bacteria, and separating and purifying the lactic acid.
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Fermentation
Lactic acid can be produced either by chemical synthesis or microbial fermentation.
Chemical synthesis of lactic acid is mainly based on the hydrolysis of lactonitrile by strong acids, and this process yields a racemic mixture of the 2 isomers, L-and D-lactic acid (Holten et al., 1971; John et al., 2007). Compare to chemical synthesis, microbial lactic acid fermentation offers an advantages in terms of the utilization of renewable cellulosic biomass, low production temperature, low energy consumption and production of pure highly optical lactic acid. Currently, almost all industries use microbial fermentation using lactic acid bacteria. The fermentation is carried under anaerobic condtions inorder to convert glucose into lactic acid instead of CO2 and H2O and it can produce two lactic acid molecules from one glucose molecule. The conventional fermentation processes for producing lactic acid from cellulosic biomass include the following four steps [7]: 1. Collection and pretreatment of cellulosic biomass. 2. Enzymatic hydrolysis: depolymerizing polysaccharides to fermentative sugars by means of hydrolic enzymes. 3. Fermentation: metabolizing the sugars to lactic acid specifically lactic acid bacteria. 4. Separation and purification: purification of lactic acid to meet the standard of comericial applications.