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    Standard Curve (Hcl+Orcinol)

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    Hana Hamid
    Experiment 1 generated a standard curve to quantify furfuraldehyde concentrations using orcinol. It measured the absorbance of solutions with varying furfuraldehyde concentrations (50-500 ppm) prepared using distilled water or 13.15% HCl. Absorbance increased linearly with concentration for both sets of solutions. The results were used to generate standard curves with high R2 values, allowing furfuraldehyde concentration to be determined based on absorbance.

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    Attribution Non-Commercial (BY-NC)
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    Standard Curve (Hcl+Orcinol)

    Uploaded by

    Hana Hamid
    13 views3 pages
    Experiment 1 generated a standard curve to quantify furfuraldehyde concentrations using orcinol. It measured the absorbance of solutions with varying furfuraldehyde concentrations (50-500 ppm) prepared using distilled water or 13.15% HCl. Absorbance increased linearly with concentration for both sets of solutions. The results were used to generate standard curves with high R2 values, allowing furfuraldehyde concentration to be determined based on absorbance.

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    biotech

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    Experiment 1 generated a standard curve to quantify furfuraldehyde concentrations using orcinol. It measured the absorbance of solutions with varying furfuraldehyde concentrations (50-500 ppm) prepared using distilled water or 13.15% HCl. Absorbance increased linearly with concentration for both sets of solutions. The results were used to generate standard curves with high R2 values, allowing furfuraldehyde concentration to be determined based on absorbance.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    13 views3 pages

    Standard Curve (Hcl+Orcinol)

    Uploaded by

    Hana Hamid
    Experiment 1 generated a standard curve to quantify furfuraldehyde concentrations using orcinol. It measured the absorbance of solutions with varying furfuraldehyde concentrations (50-500 ppm) prepared using distilled water or 13.15% HCl. Absorbance increased linearly with concentration for both sets of solutions. The results were used to generate standard curves with high R2 values, allowing furfuraldehyde concentration to be determined based on absorbance.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 3

    Experiment 1: Standard curve (13.15% HCl + 0.

    5028g furfuraldehyde + orcinol)

    Data:
    1) Blank: Distilled water
    Volume (ml)

    ppm

    reading (nm)

    Absorbance

    50

    630.0

    0.079

    10

    100

    630.0

    0.209

    15

    150

    630.0

    0.293

    20

    200

    630.0

    0.350

    25

    250

    630.0

    0.504

    30

    300

    630.0

    0.572

    35

    350

    630.0

    0.627

    40

    400

    630.0

    0.630

    45

    450

    630.0

    0.760

    50

    500

    630.0

    1.051

    graph

    2) Blank: 13.15% HCl


    Volume (ml)

    ppm

    reading (nm)

    Absorbance

    50

    630.0

    0.084

    10

    100

    630.0

    0.207

    15

    150

    630.0

    0.289

    20

    200

    630.0

    0.343

    25

    250

    630.0

    0.476

    30

    300

    630.0

    0.557

    35

    350

    630.0

    0.664

    40

    400

    630.0

    0.684

    45

    450

    630.0

    0.750

    50

    500

    630.0

    0.971

    graph

    Graph:
    1) Distilled water
    1.2

    absorbance (630 nm)

    1
    y = 0.0018x + 0.0012
    R = 0.9497
    0.8
    0.6
    0.4
    0.2
    0
    0

    100

    200

    300

    400

    500

    600

    400

    500

    600

    ppm

    2) 13.15% HCl
    1.2

    absorbance (630 nm)

    1
    y = 0.0018x + 0.0091
    R = 0.9809

    0.8

    0.6

    0.4

    0.2

    0
    0

    100

    200

    300
    ppm

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