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    Determination of Iron

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    Hironmoy Roy
    This document describes Wong's method for estimating the amount of iron in a sample. The method works by developing a red color when ferric ions react with potassium thiocyanate, and the intensity of the red color is directly proportional to the amount of iron present. Standard solutions of known iron concentration are prepared and used to create a calibration curve. Test samples are then prepared, reacted, and compared to the standard curve to determine the iron concentration in the unknown.

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    Determination of Iron

    Uploaded by

    Hironmoy Roy
    3K views2 pages
    This document describes Wong's method for estimating the amount of iron in a sample. The method works by developing a red color when ferric ions react with potassium thiocyanate, and the intensity of the red color is directly proportional to the amount of iron present. Standard solutions of known iron concentration are prepared and used to create a calibration curve. Test samples are then prepared, reacted, and compared to the standard curve to determine the iron concentration in the unknown.

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    This document describes Wong's method for estimating the amount of iron in a sample. The method works by developing a red color when ferric ions react with potassium thiocyanate, and the intensity of the red color is directly proportional to the amount of iron present. Standard solutions of known iron concentration are prepared and used to create a calibration curve. Test samples are then prepared, reacted, and compared to the standard curve to determine the iron concentration in the unknown.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    3K views2 pages

    Determination of Iron

    Uploaded by

    Hironmoy Roy
    This document describes Wong's method for estimating the amount of iron in a sample. The method works by developing a red color when ferric ions react with potassium thiocyanate, and the intensity of the red color is directly proportional to the amount of iron present. Standard solutions of known iron concentration are prepared and used to create a calibration curve. Test samples are then prepared, reacted, and compared to the standard curve to determine the iron concentration in the unknown.

    Copyright:

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    Download as DOCX, PDF, TXT or read online from Scribd
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    Estimation of Iron by WONG's Method

    To estimate the amount of iron present in the given sample by Wong's Method. PRINCIPLE : Iron is determined calorimetrically making use of fact that ferric ions give red colour with potassium thiocyanate, the intensity of red colour is directly proportional to the iron present in the given sample. The intensity of red colour is measured at 540nm. REAGENTS : 1. 30% sulfuric acid 2. 7% Potassium persulphate 3. 40% Potassium thiocyanate : 40 gms of thiocyanate is dissolved in 90 ml of distilled water, 4 ml of acetone is added and volume is made upto 100ml due to distilled water addition. 4. Stock Solution : 702.2 gm of ferrous ammonium sulphate is dissolved in 100ml of distilled water. Add 5ml of 1:1 Hcl then make up the volume to 1 litre by adding distilled water. The concentration of stock solution is 0.1 mg/ml. 5. Working Standard : 10 ml of stock solution is diluted to 100ml with distilled water. The concentration of working standard is 1mg/100ml (or) 10 micro gm/ml. PROCEDURE : STANDARDS : 1. 1ml -5ml of standard solution is pipetted out into a series of test tubes. 2. The volume is made up to 5ml by adding distilled water.

    3. Then 30% sulfuric acid is added to all the test tubes making the volume to 6ml. 4. Then 1ml of potassium persulfate solution was added. 5. Then 1.5ml of potassium thiocyanate solution is added to all the test tubes, and then incubated for 20' at room temperature. 6. The intensity of standards is observed at 540nm against blank. BLANK : 1. Blank is prepared by adding 5ml of distilled water in a test tube. 2. To this test-tube 1ml of 30% H2SO4, 1ml of potassium persulfate, and 1.5ml of potassium thiocyanate solutions are added. 3. Test Tubes are incubated for 20' at room temperature. 4. This is used as a blank. TEST : 1. Test is prepared by taking 1ml of test solution in a test-tube. 2. To this 4ml of distilled water was added. 3. Then 1ml of 30% sulfuric acid, 1ml of potassium per sulphate and 1.5ml of potassium thiocyanate solutions are added. After incubation at room temperature for 20', the intensity of the solution is observed at 540nm against blank.

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