When Doujin (the leader of the bandits on the bridge) raises his wooden hand to shoot Jubei, the details on the hand that indicate that it is man-made (ie. exposed joints) are missing from the first two shots in which it appears.
When the Eight Devils of Kimon ride to Shimoda through a forest in a storm, the animation cels of the horses are placed too far above the ground, creating an unintentional "flying" effect.
When Tessai prepares to punch Jubei, he says "the way to hell is... right here". In the Japanese version, the "right here" part of the line is heard some time after Tessai's lips move. This does not occur in the English version, where his line is in sync with his lip movements.