Poster Presentation

Helminthologia, 2009

The regional occurrence of cystic echinococcosis in slaughtered pig from Slovakia was studied in the period of 2000 -2008, along with the quantitative parameters associated with the establishment of cysts (intensity of infection, fertility, size). From 103 pig livers collected from abattoirs in 35 sites Slovakia as suspected for Echinococcus infection, 63 were positive for cystic echinococcosis, whereas in 40 livers cysticercosis was diagnosed. Fertile cysts with echinococcal protoscoleces were recorded in 25.4 % of positive pigs, with the 8.9 % fertility rate and the intensity of infection reaching value of 5.9 cysts per liver. The average size of fertile cysts was more than three times larger than diameter of sterile cysts (diameters of 4.67 cm vs. 1.37 cm, respectively). No significant differences in relative amounts of fertile and sterile cysts were found among counties (P = 0.15). The annual prevalence of E. granulosus in pigs in 2000 -2 008 has ranged from 0.02 % to 0.13 % (average rate 0.08 %), with the decreasing tendency over the period, especially after 2005. The most heavily affected areas with cystic echinococcosis over 2006 -2008 were indicated in Prešov (eastern Slovakia, 0.68 % prevalence) and Komárno districts (southwestern Slovakia, 0.26 % prevalence). Obtained data are relevant in designing regional control strategies to suppress the occurrence of disease in livestock and risk for humans to be infected.

The aim of the present study is to evaluate the presence of Cystic Echinococcosis in pigs and wild boars of Sardinia. The survey was carried out on pigs slaughtered in familiar butcheries during the years 2006-2008, while data and hydatid material from wild boars were recovered from shouted animals during the hunting campaign from 2005 to 2007. A total of 342 pigs and 461 wild boars were observed and hydatid disease was revealed respectively in 11.1% and 3.7% of the animals. Fertility was of 7.6% in pigs (68.4% of the positives animals harboured viable cysts). Thirty-nine hydatids isolates from pigs (36) and wild boar (3), were then strain typed after DNA extraction and sequencing of COI and ND partial genes. The common sheep strain was isolated in 37 pigs while the G7 or pig strain was observed in two swine. The wild boar samples harbour all to the G1 strain. Cystic Echinococcosis (CE) in pigs in Sardinia is a parasitic disease not easy indagabile for some rearing problems and also...

Cystic Echinococcosis (CE) due to the developing of one or more cysts in liver, lungs or other organs of an intermediate host after ingestion of the eggs of the Echinococcus granulosus tapeworm is an important zoonotic disease. Reports from several eastern european countries provide documented evidence for the reemergence of this disease. In the western part of Romania animals sacrificed in slaughterhouses from three counties (Arad, Timiş and Caraş-Severin) were considered. Livestock involved in the transmission pattern includes: sheep, cattle and swine. In all these counties CE maintains at a high level. The highest level was recorded in Arad County (10.49% out of all examined animals) followed by Timiş County (4.65%) and Caraş-Severin County (1.98%), respectively. Out of the three investigated species of animals, cattle were the most intensely infected (12.31% – 22.36%), with a maximum prevalence in Timiş County (22.36%), followed by sheep (5.83% – 11.51%), with a maximum prevalen...

Revista MVZ Córdoba

Objective. This study was conducted retrospectively to assess the cystic echinococcosis status of farm animals from the samples sent to Samsun Veterinary Control Institute of the Republic of Türkiye, Ministry of Agriculture and Forestry, between January 2016 and December 2022. Materials and Methods. A total of 818 sheep, 160 goats, and 990 cattle necropsy materials were examined macroscopically and histopathologically according to World Organisation for Animal Health (WOAH) guidelines. Cysts were detected in 61 of examined materials. Results. The rate of total cystic echinococcosis positivity in farm animals was found to be 3.1% in northern Türkiye. Cysts were detected in at least one of the liver and lungs. It was detected in the liver in 24 cases (39.3%), the lung in 23 cases (37.7%), and both the lung and liver in 11 cases (18%). In addition, cysts were found in both of lung and spleen (1 case), kidney and liver (1 case), and heart and liver (1 case). The positivity rate was high...

Veterinary Parasitology, 2009

Parasitology Research, 2009

A sample of 22 Echinococcus granulosus isolates collected from 12 sheep and ten humans from a focus of cystic echinococcosis in western Turkey was examined by DNA sequencing of four mitochondrial genes (cox1, atp6, nad1, rrnS). Results demonstrated the presence of two species of E. granulosus complex, E. granulosus sensu stricto and E. canadensis. Of E. granulosus sensu stricto, the G1 genotype (including three microvariants) was found in 17 isolates from humans and sheep, the G3 genotype and an intermediate form G1/G3 in one isolate each (both from sheep). Of E. canadensis, the pig strain G7 was found in three isolates from sheep and human. This is the first report of this strain in Turkey. Its presence has implications for local control programs due to its shorter maturation rate in dogs compared with E. granulosus sensu stricto. Goat and/or wild boar are likely reservoirs for G7 in the region. We provided further data on the pattern and frequency of nucleotide substitutions within the G1/G3 cluster. Based on our results and GenBank records, G2 (Tasmanian sheep strain) is not considered as a discrete genotypic unit, as its sequences at polymorphic sites conform to microvariants of both G1 and (more often) G3.

Foodborne …, 2012

An epidemiological survey of cystic echinococcosis (CE) in cattle and sheep was conducted from October 2009 to October 2011 in endemic areas of north-eastern and southern Romania. A total of 8569 animals (3043 cattle and 5526 sheep) were examined, and hydatid cysts were found in 984 (32.34%) cattle and 2756 (49.87%) sheep, respectively. The average number of cysts per animal was 11.3 (range, 1-47) in cattle and 4.2 (range, 1-16) in sheep. Of the CE-positive animals, 177 (17.9%) of the cattle and 358 (12.98%) of the sheep had hydatid cysts only in the lungs, 62 (6.3%) and 803 (29.13%) had only in the liver, and 723 (73.47%) and 1572 (57.04%), respectively, had hydatid cysts both in the liver and in the lungs. Very few animals had cysts in spleen (19 cattle and 23 sheep) and kidney (three cattle). From a sample of 422 cysts undergoing closer examination (258 from cattle, 164 from sheep), the highest fertility rate (38.41%) was found in sheep cysts, while from the cattle only four cysts (1.55%) were found to be fertile. Species identification of 13 animal isolates (nine from sheep, four from cattle) and one human isolate were done by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the mitochondrial nad1 gene and confirmed by partial sequencing of the cox1 gene. All showed the same RFLP band pattern (Echinococcus granulosus sensu stricto) and were identified as the ''sheep strain'' G1 by sequencing. The study emphasizes the hyperendemic presence of E. granulosus in Romania and outlines the necessity for the urgent development of sustainable surveillance and control strategies both in animals and humans.

The American Journal of Tropical Medicine and Hygiene, 2010