a
Food Science and Technology
ISSN 0101-2061
DDI: http://dx.doi.org/10.1590/1678-457X.6564
Physical, chemical and sensory properties of gluten-free kibbeh formulated with
millet flour (Pennisetum glaucum (L.) R. Br.)
Tcherena Amorim BRASIL1, Caroline Dário CAPITANI2*,
Katiuchia Pereira TAKEUCHI3, Tânia Aparecida Pinto de Castro FERREIRA4
Abstract
Pearl millet flour was utilized in kibbeh formulations instead of whole-wheat flour. Physicochemical properties, oxidation
stability and sensorial characteristics of control kibbeh made with whole-wheat flour (CT) were compared with kibbehs prepared
with millet flour (roasted or wet) and stored for 90 days (–18 °C). Kibbeh prepared with millet flour presented good oxidation
stability (TBARS concentration). Baked kibbehs (with roasted millet flour) presented good acceptability and kibbeh samples
did not differ significantly (p > 0.05) from the whole-wheat flour sample, when global appearance, texture and flavor were
evaluated. Millet flour could be a suitable ingredient for kibbeh formulations, maintaining their nutritional value and sensorial
quality in addition to being a gluten-free product.
Keywords: gluten-free products; alternative cereal; food stability; meat products; acceptability; roasting.
Practical Application: Kibbeh formulations with millet flour can be a good alternative for gluten-free diet.
1 Introduction
Millet (Pennisetum glaucum (L.) R. Br.) is a small cereal
with seeds of 1.2-1.8 mm in diameter and a light brown-to-brick
red colored seed coat with an undulated surface. Millet is a
word derived from “mille”, which signifies a thousand grains.
The major millet species in the world is pearl millet (Pennisetum
glaucum (L.) R. Br.), followed by foxtail, proso and finger millet
(Shahidi & Chandrasekara, 2013).
Pearl millet is a food that supplies a major proportion of calories
and protein to large segments of populations in the semi-arid
tropical regions of Africa and Asia (D’Kennedy et al., 2006).
Recombinant DNA technology is a powerful tool that has been
recently used to enhance the gene pools of sorghum and pearl
millet crops, which are regarded as important crops, particularly
in countries like Africa (D’Kennedy et al., 2006).
Millet is a gluten-free and low-cost cereal (approximately
40% lower than the price of corn), which is resistant to drought
and nutrient-poor soils (Gomes et al., 2008). In 2011, the
global millet production was about 27.5 million tones (Food
and Agriculture Drganization, 2015). Countries in Africa and
Asia produced 56% and 41% of the total world production,
respectively (Shahidi & Chandrasekara, 2013).
In years 2003/2004 millet cultivation area in Brazil
corresponded to four million hectares, and the estimative to
years 2008/2009 were about five million hectares (Brasil, 2008).
There is no current data of millet production in Brazil because
it is not commonly used for human feeding despite represents
an important ingredient for feeding confined cattle, poultry and
pork (Brasil, 2008). So, it is considered an underutilized cereal
(Suma & Urooj, 2014), even in the agroecological systems where
they grow (Shahidi & Chandrasekara, 2013).
Millet is a superior cereal with regard to nutritional quality
and presents several health benefits (Krishnan et al., 2011).
It is a rich source of dietary fiber, calcium, oleic acid (25%) and
linoleic acid (46%) (Rooney, 1978) and of phytochemicals with
nutraceutical potential (Malleshi & Hadimani, 1999). Moreover,
millet is a potent source of antioxidants, due to its phenolic
content (Dykes & Rooney, 2006; Shahidi & Chandrasekara, 2013)
and is a staple food substitute for celiac patients who require
gluten-free cereal (Shahidi & Chandrasekara, 2013).
Due to its nutritional characteristics and low cost, there is
increased interest in millet due to its health benefits, hypoglycemic
characteristics (Lakshmi Kumari & Sumathi, 2002) and due to
the antimicrobial and antioxidant activities of its polyphenols
(Chethan & Malleshi, 2007). Moreover, as millet does not contain
gluten and is known for its low carbohydrate concentration and
low glycemic index (Singh et al., 2010; Suma & Urooj, 2014),
some authors has studied its viability in bakery products such
as breads, biscuits and pasta (Rathi et al., 2004; Saha et al., 2011;
Schoenlechner et al., 2013), aiming to replace whole-wheat flour
with millet flour. The acceptability of the foods developed with
millet flour, such as biscuit dough and breads, is reported to be
very good (Saha et al., 2011; Schoenlechner et al., 2013).
Received 10 Feb., 2015
Accepted 01 May, 2015
1
Instituto Federal Goiano – IFGoiano, Rio Verde, GO, Brasil
2
Faculdade de Ciências Aplicadas – FCA, Universidade Estadual de Campinas – UNICAMP, Limeira, SP, Brasil
3
Faculdade de Nutrição – FANUT, Universidade Federal de Mato Grosso – UFMT, Cuiabá, MT, Brasil
4
Faculdade de Nutrição – FANUT, Universidade Federal de Goiás, Goiânia, GO, Brasil
*Corresponding author:
[email protected]
Food Sci. Technol, Campinas, 35(2): 361-367, Abr.-Jun. 2015
361
Gluten-free kibbeh formulated with millet flour
Unfortunately, no meat products that employ the use of
millet or millet flour have been described in the literature.
The evolution of nutritional and eating habits throughout
human history has led to the modern custom of eating snacks
(Danski, 2008). Despite originally being a Turkish cuisine, kibbeh
is one of the most famous meat snack foods in Brazil. Kibbeh
is an industrialized meat product, obtained from beef or lamb,
which is minced, added to whole-wheat flour, spices and other
ingredients (Brasil, 2000) and is usually commercialized raw
(kibbeh nayeh), deep-fried or baked. However, in its original
formulation, whole-wheat flour is an essential ingredient and
this product should not be consumed by celiac patients.
(20 ± 0.1 g) and deep fried in soybean oil (120-130 °C) for three
minutes until reaching a characteristic color (golden brown).
The baked kibbeh samples (BRM and BWM) were placed in
inox pans (30 × 20 cm) and baked in an electric oven (180 °C)
for 35 minutes. As such, we obtained the following samples:
Baked kibbeh with roasted millet flour (BRM); Fried kibbeh
with roasted millet flour (FRM); Baked kibbeh with wet millet
flour (BWM) and Fried kibbeh with wet millet flour (FWM).
Water activity analysis, microbiology assay and TBARS analyses
were performed in the defrosted samples following 90 days of
storage (–18 °C).
Thus, the aim of this study was to develop different kibbeh
formulations using millet flour and evaluate their nutritional,
sensorial and technological quality following freeze storage
(90 days at –18 °C), in order to replace whole-wheat flour and
provide novel and alternative gluten-free products.
Chemical analysis
2 Material and methods
2.1 Material and reagents
Pearl millet (Pennisetum glaucum (L.) R. Br.) - ADR 7010 cultivar,
a hybrid with the attributes of both straw and grain and great
productive potential (50 bags ha–1), was kindly provided from
the “Sementes Adriana®” Company (Mato Grosso, Brazil). Dther
ingredients used to prepare kibbeh (ground beef, whole-wheat
flour and spices) were purchased at a local market. All other
chemical reagents used in this study were of analytical grade.
Millet flour
Seeds (Pearl millet) were ground using a mill (MCS 280- 5 hp
Vieira, Tatuí, SP, Brazil) in sieves with a 3-mm particle size
under continuous suction. The millet flour was stored (–22 °C)
until analysis. Millet flour was roasted or hydrated to yield two
types of flour; roasted millet flour (RM) and wet millet flour
(WM). RM was prepared using 50 ± 0.1 g of millet flour, roasted
on a domestic stove oven at 240 °C with stirring during five
minutes. WM was prepared using 30 mL of boiling water added
to 25 ± 0.1 g of millet flour during ten minutes. The excess of
water was removed using a domestic sieve. The same hydration
procedure was used to hydrate the wet whole-wheat flour, which
was applied as a control (CT) for the kibbeh samples.
Kibbeh formulation and manufacturing
Kibbeh samples were formulated according to the Technical
Regulation of Kibbeh Identification and Quality (Brasil,
2000, 2001). The formulations were prepared as described by
Degáspari et al. (2002), with some modifications (Table 1).
TM and WM were used to replace the whole-wheat flour
in the original kibbeh formulations. All ingredients used in
these processes (Table 1) were homogenized for ten minutes
and the samples were then stored frozen for 90 days (T90)
(–18 °C). Chemical, color and texture profile analysis were
performed before and after storage for 90 days. Before the
tests, samples were previously defrosted and cooked (fried
and baked). Fried kibbeh (FRM and FWM) were portioned
362
Three samples were randomly separated from each kibbeh
sample (FRM, FWM, BRM and BWM) for the nutritional
composition analysis. Moisture, ashes and protein content were
determined according to Association of Dfficial Analytical
Chemists (1990) methods. Protein content (total nitrogen
content) was determined using the Micro-Kjeldahl method
with a conversion factor of 6.25; the digestible carbohydrates
content was determined by the difference and total lipids
extracted in methanol: chloroform (Bligh & Dyer, 1959).
Dietary fiber was determined according to the methodology
described by Prosky et al. (1988). Water activity (aw) was
measured during 90 days of storage using the Aqualab system
(Decagon, model CX-2, Devices Inc., Pullman WA, USA) at
room temperature (25 °C).
Microbiological analyses
The ingredients used in the kibbeh formulations, fried
and baked kibbeh samples were submitted to microbiological
analysis to control their sanitary quality, according to Resolution
RDC number 12 of the National Health Surveillance Agency
(Brasil, 2001). Analyses were performed in triplicate as described
by the Compendium of Methods for the Microbiological
Examination of Foods (Downes & Ito, 2001).
Table 1. Kibbeh Formulation (g.100g–1).
Ingredients
Beef
Whole-wheat
flour
Wet millet flour
Roasted millet
flour
Fresh garlic
Fresh parsley
Fresh mint
Salt
Soybean oil
Total (g)
CT
85.00
8.50
0.34
0.70
1.36
1.40
2.70
100.00
Kibbeh Samplesa
RM
85.00
-
WM
85.00
-
8.50
8.50
-
0.34
0.70
1.36
1.40
2.70
100.00
0.34
0.70
1.36
1.40
2.70
100.00
a
All samples were fried (F) or baked (B) for the analysis. CT: Control (whole-wheat flour).
RM: Roast Millet Flour. WM: Wet Millet Flour.
Food Sci. Technol, Campinas, 35(2): 361-367, Abr.-Jun. 2015
Brasil et al.
Color and texture analyses
Statistical analysis
The samples were defrosted, except T0, and cooked (fried
or baked) prior to starting color and the texture analysis. Color
parameters were analyzed using a colorimeter (HunterLab,
ColorQuest II, Reston, England) with Universal Software 4.10 on
the CIELAB system. The color measurements were performed five
times in different places for each sample. The texture parameters was
evaluated using a texturometer (Stable MicroSystems, TA-XTplus,
Surrey, UK) with SMS-P/5 aluminum probe under a uniaxial
force up to 70% strain, using cylindrical specimens of size
2 × 2 cm, with 2 mm s–1 pre-test speed, 1 mm s–1 test speed, and
10 mm s–1 post-test speed. Hardness, defined as the maximum
peak force required to compress the sample, was evaluated and
expressed in Newtons (N). Seven measurements were recorded
for each sample.
The data were tested for normality (Shapiro-Wilk test), then
submitted to analysis of variance (two-way ANDVA), followed
by Bonferroni post-tests, to compare the replicate means by
samples. Samples from the two time intervals (T0 and T90) were
compared by repeated ANDVA measurements. Chemical and
sensorial results were submitted to analysis of variance (one-way
ANDVA). All statistical analyses and graphs were made using
GraphPad Prism software (version 5.00Trial, Targetware
Informática Ltda, São Paulo, Brazil).
Lipid oxidation
Lipid oxidation was assessed by measuring the thiobarbituric
acid reactive substances (TBARS), according to the methodology
described by Wyncke (1970) with some adaptations proposed
by Capitani et al. (2009). TBARS concentration was measured
after the storage time (90 days) after defrosting the samples.
A calibration curve was constructed using TEP solutions of
concentrations varying from 0 to 1.25 mg.mL–1. The TBARS
concentration was expressed as mg MDA.kg–1 kibbeh.
Sensory evaluation
Sensorial analysis was conducted in two steps. First, the
samples were evaluated using the preference test based on a
nine-point hedonic scale (1= extremely dislike to 9= extremely
like) (Meilgaard et al., 2007). Global appearance characteristics
were evaluated and the acceptability index (AI) of the kibbeh
samples was calculated as AI (%) = (Average score ÷ highest score)
x 100 (Teixeira et al., 1987). The sensorial test was applied in
individual cabins illuminated by red lamps. Fresh kibbeh samples
(fried and baked) were served on individual plates (20 ± 0.1 g),
coded with a three-digit number, following a randomized block
design, to 57 untrained volunteer panelists from the University
staff. Panelists signed an approved informed consent form
regarding the Ethics Protocols of Research involving Human
Tests before starting the test (Research Ethics Committee of
Federal University of Goiás – no 209/2011). Panelists aged
between 20 and 64 years were selected to taste and analyse the
samples. Were used as exclusion criteria; diabetes, allergies to
the ingredients of the kibbeh and diseases that could interfere
with taste and/or olfactory sensitivity. The second step of the
study was carried out in a supermarket during three days using
potential consumers (n = 92), aiming to evaluate the acceptability
of the potential consumers. The baked kibbeh samples (CT, BRM
and BWM) were freshly prepared using the same conditions
described above and heated (35 °C) before the consumer test.
Samples were evaluated using the preference test, based on a
nine-point hedonic scale (1= extremely dislike to 9= extremely
like) (Meilgaard et al., 2007); taste, texture, and global appearance
characteristics were evaluated.
Food Sci. Technol, Campinas, 35(2): 361-367, Abr.-Jun. 2015
3 Results
Millet flour maintained the same amount (p>0.05) of dietary
fiber (4.06 ± 0.11 g.100g–1) and protein (20.60 ± 2.21 g.100g–1)
as whole-wheat flour (protein: 23.54 ± 2.15 g.100g–1; dietary
fiber: 3.21 ± 0.06 g.100g–1). The average chemical composition
of kibbeh samples prepared with millet flour (RM or WM) were
55.91 ± 2.04 g moisture, 12.51 ± 1.76 g lipids and 2.80 ± 0.27 g
ashes.100g–1 of sample. These results were not different (p<0.05)
of the kibbeh prepared whole-wheat flour (CT), that presented
56.06 ± 0.98 g moisture; 10.06 ± 0.73 g lipids and 1.34 ± 0.13 g
ashes.100g–1 of sample.
The water activity parameter did not present significant
changes following 90 days of storage and the average results
ranged between 0.96 and 0.99. While the kibbehs had high water
activity values, microbiological analysis showed satisfactory
sanitary conditions for all of the kibbeh samples and were
below the limits established by the Brazilian sanitary legislation
(<10 UFC.g–1), representing accordance with the law of good
manufacturing practices (Brasil, 2001).
After 90 days of storage (T0 and T90) significant differences
(p<0.05) in the color parameters of the kibbeh samples were
observed for the L*, a*, b* and Chroma (Table 2). Reductions
in the lightness (L*) and redness (a*) parameters were recorded
following 90 days of storage. FRM and CT fried samples showed
a greater reduction in the L* parameter (ΔL* ~ 24.5 and 20.7,
respectively), indicating increased opacity during storage.
The texture analysis showed significant differences (p<0.05) in
hardness for almost samples following the storage time (90 days),
except for the Control fried (Fried kibbeh with whole-wheat
flour - CT fried) (Figure 1). Initially, samples prepared with
roasted millet flour (FRM and BRM) were harder than other
samples and this may be due to the lower water content in the
RM. However freeze storage reduced the hardness (N) in almost
samples (p<0.05), except for the Control fried (Fried kibbeh
with whole-wheat flour - CT fried) and was more expressive
for the FWM sample (T0 = 132.7 ± 11.7; T90 = 72.7 ± 4.6).
The CT baked sample did not differ significantly (p>0.05) from
the CT fried after 90 days of storage.
The data for the oxidative stability of the kibbeh samples
(Figure 2) reveal that the CT sample presented an increased TBARS
concentration following 90 days of storage (–18 °C), where the
TBARS concentration significantly increased (p<0.05) during
freezing for 90 days (T0 and T90) at –18 °C. When ascorbic acid
was added to the control sample (0.01%), values of TBARS were
not lowered when compared to those of the CT (without ascorbic
363
Gluten-free kibbeh formulated with millet flour
Table 2. Color Parameters (L*, a*, b*, and Chroma) of Fresh Kibbeh (T0) and following 90 days of freeze storage (T90), based on the CIELAB
System. Results were expressed as medium ± SD (n = 5).
L*
Samples
T0
CT fried
CT baked
FWM
FRM
BWM
BRM
46.6 ± 0.1
50.4 ± 0.1a
36.1 ± 0.1a,b
47.7 ± 0.5a,b,c
44.8 ± 3.5a,c,d
45.8 ± 0.7a,b,c,d,e
T90
T0
Color parameters
a*
T90
25.9 ± 2.0
26.3 ± 0.3
38.6 ± 2.4a
24.9 ± 0.1a
21.6 ± 3.0a,b
36.0 ± 0.1a,b
23.2 ± 2.4b,c
30.9 ± 0.2a,b,c
34.9 ± 2.7a,b,c,d 24.2 ± 0.4b,c,d
33.1 ± 1.5a,c,d 23.1 ± 0.3a,b,c,d,e
11.3 ± 0.5
6.3 ± 0.4a
17.7 ± 1.1a,b
11.8 ± 0.8b,c
5.0 ± 0.9a,b,c,d
5.5 ± 0.6a,b,c,d
b*
T0
16.1 ± 1.8
7.1 ± 0.6a
44.1 ± 0.6a,b
31.2 ± 0.9a,b,c
14.0 ± 0.5a,b,c,d
21.3 ± 0.8a,b,c,d,e
Chroma
T90
18.5 ± 1.3
17.9 ± 1.2
28.9 ± 1.4a,b
17.9 ± 1.1c
16.7 ± 0.8a,c
16.7 ± 0.6a,c
T0
T90
30.8 ± 1.2
25.9 ± 0.1a
57.0 ± 1.8a,b
43.9 ± 0.8a,b,c
27.9 ± 0.3a,b,c,d
26.6 ± 0.5a,c,d
21.7 ± 1.1
19.1 ± 1.2a
33.9 ± 1.2a,b
21.5± 1.4b,c
17.5± 1.0a,b,c,d
17.6 ± 0.6a,c,d
Different letters in the same columns represent significant difference (p < 0.05) between the samples. CT fried: Control fried (Fried kibbeh with whole wheat flour ). CT baked: Control
baked (Baked kibbeh with whole wheat flour). FWM: Fried kibbeh with wet millet flour. FRM: Fried kibbeh with roasted millet flour. BWM: Baked kibbeh with wet millet flour. BRM:
Baked kibbeh with roasted millet flour.
acid) and the millet samples (Figure 2). Samples prepared with
millet flour (RM and WM), showed lower TBARS concentrations
compared to the CT after 90 days of storage (p<0.05).
There were no statistical differences among the samples
(p>0.05) when the acceptability indices (AI %) were analyzed.
Untrained panelists preferred the baked CT sample (AI = 83%),
followed by BRM (78.9%) and fried CT (75.4%). When potential
consumers evaluated the baked kibbeh samples in the supermarket,
no differences (p<0.05) were found between the CT sample and the
other samples (BRM and BWM). All attributes received average
grades of above six, indicating good acceptability compared with
conventional preparations (CT).
Figure 1. Hardness (N) of the baked (B) or fried (F) kibbeh samples
at the beginning (T0) and after 90 days of freeze storage (T90). Bars
represent means (n = 7 + SD). Bars labelled with different letters
represents statistical difference (p < 0.05): Capital letter: comparison
between samples at T0 or T90 of storage, for same kibbeh formulation;
Small letter: comparison among all kibbeh formulations at same storage
time. CT fried: Control1 (Fried kibbeh with whole-wheat flour), CT
baked: Control2 (Baked kibbeh with whole-wheat flour), FWM: Fried
kibbeh with wet millet flour, FRM: Fried kibbeh with roasted millet
flour, BWM: Baked kibbeh with wet millet flour, BWM: Baked kibbeh
with roasted millet flour.
Figure 2. Lipid oxidation of the samples at the beginning (T0) and
after 90 days of freeze storage (T90), expressed as mg MDA.kg–1 sample.
Bars represent mean (n = 3) ± SD. Bars followed by different letters
represents statistical difference (p < 0.05) between samples. CT ADX:
kibbeh prepared with whole whole-wheat flour and with ascorbic
acid (0.01% p:v); CT: kibbeh prepared with whole-wheat flour and
without antioxidant; WM: kibbeh prepared with wet millet flour;
RM: kibbeh prepared with roasted millet flour.
364
4 Discussion
The nutritional composition of kibbehs prepared with
millet flour were not different to the nutritional contents of
the CT kibbeh samples. However, lipids content were slightly
higher in the kibbehs prepared with millet flour and it could
be explained mainly by the influence of the cooking procedure
(Krishnan et al., 2011; Miyagusku, 2006), since all preparations
used the same ingredients, with the exception of the millet. Despite
these results, all kibbeh samples prepared with millet flour could
be classified as a “source” and as a “food with high amount” of
fiber and proteins (Brasil, 2012) due to their elevated amount of
fiber (average 4.0 g.100g–1) and proteins (average 20.6 g.100g–1).
Thus, millet flour could represent an alternative to substitute
whole-wheat flour, ensuring the protein and dietary fiber intake
for individuals with celiac disease.
In this study, we did not use sodium erythorbate to preserve
or to fix the red color of the meat; the color of kibbehs is
usually brown or dark, and we observed the reduction in the
lightness (L*) and redness (a*) parameters during 90 days of
storage. Usually, freeze storage (–18 °C) of beef samples reduced
colorimetric parameters over storage time (Fernández et al., 2007;
Scramlin et al., 2010). The excess of metamyoglobin, formed
during freeze storage, could reduce the redness parameter (a*)
and, consequently, increase b* values (Martínez et al., 2006;
Valencia et al., 2008). However, the b* parameter was significantly
reduced in the FRM and BWM samples and these results did
not corroborate with the literature. The changes in the lightness
(L*) and in the redness (a*) values may not indicate loss in
Food Sci. Technol, Campinas, 35(2): 361-367, Abr.-Jun. 2015
Brasil et al.
the quality parameters, unless the consumer disapproves this
parameter when buying these products. However, there were
no sensorial differences when color was analyzed.
Different cooking methods (Apple et al., 1999) influenced
the texture of the food products; however, no differences between
the hardness (N) of the baked or fried kibbeh were found in this
study. According to the sensorial analysis, panelists related that
the kibbeh samples prepared with RM flour were crisper and
harder than the other preparations and the texture profiles confirm
these findings. Following storage (90 days), the kibbeh samples
demonstrated reduced hardness due to ice crystal formation by
the meat products during freeze storage, which promotes the
output of intracellular water by osmosis and causes modification
in texture parameters after the defrosting process (Drdónez,
2005). Considering formulations containing millet, Baked
kibbeh (BWM and BRM) showed lower reduction of hardness
than fried kibbeh (FWM and BRM) in relation to initial time
and after storage under freezing. Despite of kibbeh containing
whole-wheat flour (CT baked and CT fried) have showed lower
hardness than kibbeh containing millet flour, consumers did not
detected differences in the texture, because sensory acceptability
showed no statistical differences among the samples (p>0.05)
when the acceptability indices (AI %) were analyzed.
Ascorbic acid (applied at the 0.01% level) may have acted as
a pro-oxidant in the CT samples, resulting in increased TBARS
concentrations following storage. Moreover, antioxidant efficiency
depends on the level of the antioxidant in the matrix composition
(Capitani et al., 2013). In the other samples prepared with millet
flour (RM and WM), TBARS concentration remained below
the limits (<0.51 mg MDA.kg–1 kibbeh) during 90 days of
storage, and this would suggest that there was no development
of a rancid flavored (0.5 to 2.0 mg MDA.kg–1) sample (Gray
& Pearson, 1984).
Some studies have reported antioxidant and potent radical
scavenging activity in millet grains (Hegde et al., 2005; Dykes &
Rooney, 2006; Amadou et al., 2013a; Shahidi & Chandrasekara,
2013; Pradeep & Sreerama, 2015) and the in vitro antioxidant
activity of millet is usually attributed to its phenolic content,
represented by vanillic and ferulic acids (Pradeep & Sreerama, 2015).
More recently, Zhang & Liu (2015), observed that foxtail millet
extracts inhibited the growth of human breast and liver cancer
cells in culture. While the antioxidant activity and phenolic
content of samples were not evaluated in this study, it is
important to note that the presence of millet flour maintained
TBARS levels lower during 90 days of storage. The antioxidant
properties of millet grains could also provide health benefits to
humans (Hegde et al., 2005; Amadou et al., 2013b) acting on
management of cardiovascular, geriatric diseases and cancers
(Zhang & Liu, 2015). The use of millet as a nutraceutical and
specialty food is warranted (Shahidi & Chandrasekara, 2013)
and should be studied in humans’ subjects to observe the impact
of this food on health.
Dur results corroborate with other studies reporting
good acceptability of products formulated with millet flour
(Krishnan et al., 2011; Saha et al., 2011; Schoenlechner et al., 2013).
According to Singh et al. (2012), millets can replace wheat in
the bread to some extent with good acceptability. However,
Food Sci. Technol, Campinas, 35(2): 361-367, Abr.-Jun. 2015
the addition of millet flour to wheat bread caused a decline in
bread quality due to the reduction in the final gluten content
(Schoenlechner et al., 2013), which is essential to breads.
5 Conclusions
Roasted or wet millet flour could be used into kibbeh
formulations to replace whole-wheat flour without affecting their
nutritional and technological properties. Kibbeh formulations
with roasted or wet millet flour presented good acceptability and
showed oxidation stability measured by TBARS concentration
during storage for 90 days at –18 °C. So, millet holds a huge
potential for food production and, in particular, for replacing
whole-wheat flour. As such, it can be used in several food
products, such as kibbeh, making a valuable contribution to
a healthy diet, particularly in individuals with celiac disease.
Acknowledgements
We would like to thank the Postgraduate Program in Nutrition
and Health of Federal University of Goiás for their financial
support and the Adriana Seed® Company for the supply of millet.
References
Amadou, I., Le, G. W., & Shi, Y. H. (2013b). Evaluation of antimicrobial,
antioxidant activities and nutritional values of fermented foxtail
millet extracts by Lactobacillus paracasei Fn032. International
Journal of Food Properties, 16(6), 1179-1190. http://dx.doi.org/10.
1080/10942912.2011.579673.
Amadou, I., Le, G.W., Amza, T., Sun, J., & Shi, Y. H. (2013a). Purification
and characterization of foxtail millet-derived peptides with antioxidant
and antimicrobial activities. Food Research International, 51(1), 422428. http://dx.doi.org/10.1016/j.foodres.2012.12.045.
Apple, J. K., Rakes, L. K., & Watson, H. (1999). Cooking and shearing
methodology effects on warmer-Bratzler shear force values of
pork. Journal of Muscle Foods, 10(3), 269-277. http://dx.doi.
org/10.1111/j.1745-4573.1999.tb00402.x.
Association of Dfficial Analytical Chemists – ADAC (1990). Official
methods of analysis. Washington, DC: ADAC.
Bligh, E. G., & Dyer, W. J. (1959). A rapid method of total lipid extraction
and purification. Canadian Journal of Biochemistry and Physiology,
37, 911-917. http://dx.doi.org/10.1139/o59-099. PMid:13671378
Brasil, Agência Nacional de Vigilância Sanitária – ANVISA. (2001,
January 1). Resolução RDC nº 12, de 02 de janeiro de 2001. Aprova
o Regulamento Técnico sobre padrões microbiológicos para alimentos.
Diário Dficial da República Federativa do Brasil. Retrieved from
http://portal.anvisa.gov.br/wps/wcm/connect/a47bab8047458b909
541d53fbc4c6735/RDC_12_2001.pdf?MDD=AJPERES
Brasil, Ministério da Agricultura, Pecuária e Abastecimento, Empresa
Brasileira de Pesquisa Agropecuária – EMBRAPA, Centro Nacional
de Pesquisa de Milho e Sorgo. (2008). A cultura do Milheto. Retrieved
from http://www.infoteca.cnptia.embrapa.br/infoteca/bitstream/
doc/491152/1/Milheto.pdf
Brasil, Ministério da Agricultura, Secretaria Nacional de Defesa Agropecuária.
(2000). Regulamento técnico de identidade e qualidade de Kibbeh.
Brasília, DF: MAPA. Retrieved from http://www.cfmv.org.br/portal/
legislacao/outras_normas/instrucao_normativa_020_MAA.htm
Brasil, Ministério da Saúde, Secretaria de Vigilância Sanitária. (2012).
RDC nº 54, de 12 de novembro de 2012. Dispõe sobre o regulamento
365
Gluten-free kibbeh formulated with millet flour
técnico sobre informação nutricional complementar. Retrieved http://
portal.anvisa.gov.br/wps/wcm/connect/630a98804d7065b981f1e1
c116238c3b/Resolucao+RDC+n.+54_2012.pdf?MDD=AJPERES
Capitani, C. D., Carvalho, A. C., Rivelli, D. P., Barros, S. B. M., & Castro,
I. A. (2009). Evaluation of natural and synthetic compounds according
to their antioxidant activity using a multivariate approach. European
Journal of Lipid Science and Technology, 111(11), 1090-1099. http://
dx.doi.org/10.1002/ejlt.200800215.
Capitani, C. D., Hatano, M. K., Marques, M. F., & Castro, I. A. (2013).
Effects of optimized mixtures containing phenolic compounds on
the oxidative stability of sausages. Food Science And Technology
International = Ciencia Y Tecnología De Los Alimentos Internacional,
19(1), 69-77. http://dx.doi.org/10.1177/1082013212442184.
PMid:23014857
Chethan, S., & Malleshi, N. G. (2007). Finger millet polyphenols:
characterization and their nutraceutical potential. American Journal
of Food Technology, 2(7), 582-592. http://dx.doi.org/10.3923/
ajft.2007.582.592.
Danski, M. T. R. (2008). Historia y alimentacion: el advent del fast food
en Curitiba. Historia Actual Online, 17(1), 19-29.
Degáspari, C. H., Piccolomini, A. F., & Picanço, M. C. A. (2002).
Desenvolvimento de produto à base de fibras de beterraba (Beta
vulgaris variedade açucareira). Alimentos e Nutrição, 13(1), 103-115.
Downes, F. P., & Ito, K. (Eds.). (2001). Compendium of methods for the
microbiological examination of foods (4th ed.). Washington: American
Public Health Association. http://dx.doi.org/10.2105/9780875531755.
Dykes, L., & Rooney, L. W. (2006). Review: sorghum and millet phenols
and antioxidants. Journal of Cereal Science, 44(3), 236-251. http://
dx.doi.org/10.1016/j.jcs.2006.06.007.
Fernández, P. P., Sanz, P. D., Molina-García, A. D., Dtero, L., Guignon, B.,
& Vaudagna, S. R. (2007). Conventional freezing plus high pressurelow temperature treatment: Physical properties, microbial quality
and storage stability of beef meat. Meat Science, 77(4), 616-625.
http://dx.doi.org/10.1016/j.meatsci.2007.05.014. PMid:22061950
Food and Agriculture Drganization – FAD. (2015). FAO Statistical
Databases. Retrieved from http://www.fao.org
Gomes, P. C., Rodrigues, M. P., Albino, L. F. T., Rostagno, H. S., Gomes,
M. F. M., Mello, H. H. C., & Brumano, G. (2008). Determinação
da composição química e energética do milheto e sua utilização
em rações para frangos de corte de 1 a 21 dias de idade. Revista
Brasileira de Zootecnia, 37(9), 1617-1621. http://dx.doi.org/10.1590/
S1516-35982008000900013.
Gray, J. I., & Pearson, A. M. (1984). Cured meat flavor. Advances in Food
Research, 29, 1-86. http://dx.doi.org/10.1016/S0065-2628(08)60055-5.
Hegde, P. S., Rajasekaran, N. S., & Chandra, T. S. (2005). Effects of
the antioxidant properties of millet species on oxidative stress and
glycemic status in alloxan-induced rats. Nutrition Research, 25(12),
1109-1120. http://dx.doi.org/10.1016/j.nutres.2005.09.020.
Krishnan, R., Dharmaraj, U., Sai Manohar, R., & Malleshi, N. G. (2011).
Quality characteristics of biscuits prepared from finger millet seed
coat based composite flour. Food Chemistry, 129(2), 499-506. http://
dx.doi.org/10.1016/j.foodchem.2011.04.107.
Lakshmi Kumari, P., & Sumathi, S. (2002). Effect of consumption of
finger millet on hyperglycemia in non-insulin dependent diabetes
mellitus (NIDDM) subjects. Plant Foods for Human Nutrition,
57(3-4), 205-213. http://dx.doi.org/10.1023/A:1021805028738.
PMid:12602929
Malleshi, N. G., & Hadimani, N. A. (1999). Nutritional and technological
characteristics of small millets and preparation of value added
products from them. In K. W. Riley, S. C. Gupta, A. Seetharam & J.
366
N. Mushonga (Eds.), Advances in small millets (pp. 270–287). New
Delhi: Dxford/IBH publishing Company.
Martínez, L., Djenane, D., Cilla, I., Beltrán, J. A., & Roncáles, P. (2006).
Effect of varying oxygen concentrations on the shelf-life of fresh
pork sausages packaged in modified atmosphere. Food Chemistry,
94(2), 219-225. http://dx.doi.org/10.1016/j.foodchem.2004.11.007.
Meilgaard, M., Civille, G. V., & Carr, B. T. (2007). Sensory evaluation
techniques (4th ed.). Florida, USA: CRC Press. 448 p.
Miyagusku, L. (2006). Rotulagem de alimentos segundo ANVISA:
composição de carnes in natura. In C. J. C. Castillo (Ed.), Qualidade
de carne (pp. 209-219). São Paulo: Varela.
D’Kennedy, M. M., Grootboom, A., & Shewry, P. R. (2006). Harnessing
sorghum and millet biotechnology for food and health. Journal of Cereal
Science, 44(3), 224-235. http://dx.doi.org/10.1016/j.jcs.2006.08.001.
Drdónez, J. A. (2005). Tecnologia de alimentos: componentes dos alimentos
e processos. Porto Alegre: Artmed.
Pradeep, P. M., & Sreerama, Y. N. (2015). Impact of processing on the
phenolic profiles of small millets: evaluation of their antioxidant
and enzyme inhibitory properties associated with hyperglycemia.
Food Chemistry, 169, 455-463. http://dx.doi.org/10.1016/j.
foodchem.2014.08.010. PMid:25236251
Prosky, L., Asp, N. G., Schewizer, T. F., DeVries, J. W., & Furda, I. (1988).
Determination of insoluble, soluble, and total dietary fibre in foods
and food products: interlaboratory study. Journal - Association of
Official Analytical Chemists, 71, 1017-1023. PMid:2853153.
Rathi, A., Kawatra, A., & Sehgal, S. (2004). Influence of depigmentation
of pearl millet (Pennisetum glaucum L.) on sensory attributes,
nutrient composition, in vitro protein and starch digestibility of
pasta. Food Chemistry, 85(2), 275-280. http://dx.doi.org/10.1016/j.
foodchem.2003.06.021.
Rooney, L. W. (1978). Sorghum and pearl millet lipids. Cereal Chemistry,
55(5), 584-590.
Saha, S., Gupta, A., Singh, S. R. K., Bharti, N., Singh, K. P., Mahajan,
V., & Gupta, H. S. (2011). Compositional and varietal influence of
finger millet flour on rheological properties of dough and quality of
biscuit. LWT - Food Science and Technology, 44(3), 616-621. http://
dx.doi.org/10.1016/j.lwt.2010.08.009.
Schoenlechner, R., Szatmari, M., Bagdi, A., & Tömösközi, S. (2013).
Dptimization of bread quality produced from wheat and proso millet
(Panicum miliaceum L.) by adding emulsifiers, transglutaminase
and xylanase. LWT - Food Science and Technology, 51(1), 361-366.
http://dx.doi.org/10.1016/j.lwt.2012.10.020.
Scramlin, S. M., Newman, M. C., Cox, R. B., Sepe, H. A., Alderton, A.
L., D’Leary, J., & Mikel, W. B. (2010). Effects of oregano oil brine
enhancement on quality attributes of beef longissimus dorsi and
semimembranosus muscles from various age animals. Journal of
Food Science, 75(2), S89-S94. http://dx.doi.org/10.1111/j.17503841.2009.01459.x. PMid:20492261
Shahidi, F., & Chandrasekara, A. (2013). Millet grain phenolics and
their role in disease risk reduction and health promotion: a review.
Journal of Functional Foods, 5(2), 570-581. http://dx.doi.org/10.1016/j.
jff.2013.02.004.
Singh, K. P., Mishra, A., & Mishra, H. N. (2012). Fuzzy analysis of
sensory attributes of bread prepared from millet-based composite
flours. LWT - Food Science and Technology, 48(2), 276-282. http://
dx.doi.org/10.1016/j.lwt.2012.03.026.
Singh, K. P., Mishra, H. N., & Saha, S. (2010). Moisture-dependent properties
of barnyard millet grain and kernel. Journal of Food Engineering,
96(4), 598-606. http://dx.doi.org/10.1016/j.jfoodeng.2009.09.007.
Food Sci. Technol, Campinas, 35(2): 361-367, Abr.-Jun. 2015
Brasil et al.
Suma, P. F., & Urooj, A. (2014). Nutrients, antinutrients & bioaccessible
mineral content (in vitro) of pearl millet as influenced by milling.
Journal Food Science and Technology, 51(4), 756-761. http://dx.doi.
org/10.1007/s13197-011-0541-7. PMid:24741171
Teixeira, E., Meinert, E. M., & Barbetta, P. A. (1987). Análise sensorial
de alimentos. Florianópolis: UFSC.
Valencia, I., D’Grady, M. N., Ansorena, D., Astiasarán, I., & Kerry, J. P.
(2008). Enhancement of the nutritional status and quality of fresh
pork sausages following the addition of linseed oil, fish oil and
Food Sci. Technol, Campinas, 35(2): 361-367, Abr.-Jun. 2015
natural antioxidants. Meat Science, 80(4), 1046-1054. http://dx.doi.
org/10.1016/j.meatsci.2008.04.024. PMid:22063835
Wyncke, W. (1970). Direct determination of the thiobarbituric acid
value in trichloroacetic acid extracts of fish as a measure of oxidative
rancidity. Fett Wissenschaft Technologie, 72(12), 1084-1087.
Zhang, L. Z., & Liu, R. H. (2015). Phenolic and carotenoid profiles and
antiproliferative activity of foxtail millet. Food Chemistry, 174, 495-501.
http://dx.doi.org/10.1016/j.foodchem.2014.09.089. PMid:25529711
367