SMRT sequencing data for Garcinia mangostana L. variety Mesta

Garcinia mangostana L. (Mangosteen), of the family Clusiaceae, is one of the economically important tropical fruits in Indonesia. In the present study, we performed de novo transcriptomic analysis of Garcinia mangostana L. through RNA-Seq technology. We obtained the raw data from 12 libraries through Ion Proton System. Clean reads of 191,735,809 were obtained from 307,634,890 raw reads. The raw data obtained in this study can be accessible in DDBJ database with accession number of DRA005014 with bioproject accession number of PRJDB5091. We obtained 268,851 transcripts as well as 155,850 unigenes, having N50 value of 555 and 433 bp, respectively. Transcript/unigene length ranged from 201 to 5916 bp. The unigenes were annotated with two main databases from NCBI and UniProtKB, respectively having annotated-sequences of 73,287 and 73,107, respectively. These transcriptomic data will be beneficial for studying transcriptome of Garcinia mangostana L.

Euphytica, 2000

The novel molecular marker technique Randomly Amplified DNA Fingerprinting (RAF) was used to survey genetic relationships between 37 accessions of the tropical fruit G. mangostana (mangosteen) and among 11 accessions from eight other Garcinia species. Although mangosteen is believed to reproduce exclusively through apomixis, our results show that considerable genetic diversity exists within G. mangostana and between other Garcinia species. Among the 37 G. mangostana accessions examined, nine different genotypes were identified which clustered into three distinct groups based on correspondence analysis (reciprocal averaging). For 26 (70%) of the accessions no marker variation was detected over 530 loci screened. A further eight (22%) accessions exhibited very low levels of variation (0.2-1%) suggesting at least one well conserved mangosteen genotype. The remaining three accessions (8%) showed extensive variation (22-31%) compared with the majority of accessions. The three mangosteen groups were 63-70% dissimilar to the other Garcinia species investigated. The genetic diversity identified in this research will assist in the conservation of Garcinia germplasm and provides a valuable framework for the genetic improvement of mangosteen.

Mango (Mangifera indica L.) is known as the 'king of fruits' for its rich taste, flavor, color, production volume and diverse end usage. It belongs to plant family Anacardiaceae and has a small genome size of 439 Mb (2n = 40). Ancient literature indicates origin of cultivated mango in India. Although wild species of genus Mangifera are distributed throughout South and SouthEast Asia, recovery of Paleocene mango leaf fossils near Damalgiri, West Garo Hills, Meghalaya point to the origin of genus in peninsular India before joining of the Indian and Asian continental plates. India produces more than fifty percent of the world's mango and grows more than thousand varieties. Despite its huge economic significance genomic resources for mango are limited and genetics of useful horticultural traits are poorly understood. Here we present a brief account of our recent efforts to generate genomic resources for mango and its use in the analysis of genetic diversity and population structure of mango cultivars. Sequencing of leaf RNA from mango cultivars 'Neelam', 'Dashehari' and their hybrid 'Amrapali' revealed substantially higher level of heterozygosity in 'Amrapali' over its parents and helped develop genic simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers. Sequencing of double digested restriction-site-associated genomic DNA (ddRAD) of 84 diverse mango cultivars identified 1.67 million high quality SNPs and two major sub-populations. We have assembled 323 Mb of the highly heterozygous 'Amrapali' genome using long sequence reads of PacBio single molecule real time (SMRT) sequencing chemistry and predicted 43,247 protein coding genes. We identified in the mango genome 122,332 SSR loci and developed 8,451 Type1 SSR and 835 HSSR markers for high level of polymorphism. Among the published genomes, mango showed highest similarity with sweet orange (Citrus sinensis). These genomic resources will fast track the mango varietal improvement for high productivity, disease resistance and superior end use quality.

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International Journal of Communication, 2018

This article describes the newest stage in the development of modern technological media. I call this stage “media analytics.” It follows the previous stages of massive reproduction (1500–), broadcasting (1920–), the use of computers for media creation workflows (1981–), the Web as global content creation and distribution network (1993–), and social media platforms (2004–), to name just a few such stages. Unlike other stages, the new stage is not focused on new mechanisms for creation, publishing, or distribution of media, although it also affects these operations. Instead, this new stage is about automatic computational analysis of the content of all online digital media, personal online behaviors and communication, and automatic actions based on this analysis. Media analytics is the new stage of media technology that impacts everyday cultural experiences of significant percentages of populations in dozens of countries who use Internet and computing devices. (For figures about use of Internet and social media in the USA by different demographics groups, see latest Pew Research Center Internet & Tech reports.) (…) While media analytics technologies and concepts are widely discussed in computer and data sciences in business publications, in conferences and trade shows, in leading science journals, and being taught to millions of students worldwide in computer science and data science classes, they are not discussed in either popular press or by academics outside of technology and science fields. This lack of systematic knowledge on the part of many academics and journalists who write about digital cultures about the details of the computational processes that drive web services, apps, desktop applications, video games, search, image detection, voice recognition, recommendation systems, behavioral advertising, and so on, as well as contemporary software engineering and the field of data science in general often prevents them, in my view, from seeing the full picture. (Understanding of many of these details does require knowledge of computer science, and today very few people in academic humanists, social sciences or journalism have this background.)

En el presente análisis de investigación se entregan herramientas al detectarse la necesidad de estandarizar los procesos de gestión en vías de una implementación de la Norma ISO 9001:2000, en la empresa PRESERCON LTDA. (Prestación de Servicios de Personal y Construcción Limitada), la cual presta servicios de personal a su principal cliente que es la Compañía Siderúrgica Huachipato S.A. Debido a la importancia que tiene para la empresa el mejorar la atención al cliente, el cual tiene gran presencia a nivel internacional, ya que exporta materia prima a importantes países. Por lo mismo, el cliente de la empresa PRESERCON LTDA. Debería tener a todo su personal capacitado y certificado, para una mayor productividad y al igual que esta se encuentra integrada a las empresas certificadas a nivel nacional y mundial. El principal objetivo al implementar esta norma es mejorar las problemáticas que se presentan en la empresa, y mejorando estos aspectos, tener una mejor retroalimentación con el cliente y así poder satisfacer mejor las necesidades del mismo. Al implementar esta norma la empresa, participa de un mercado agresivo, competitivo, pero aun mas competitivo, lo que la deja con una ventaja, frente a otras empresas del mismo rubro, al igual que satisfaciendo al cliente y por sobre todo manteniéndose en el tiempo, el cual es su principal objetivo.

As pointed out by V. Bellelli (2013), the ‘definitive’ solution proposed by Pallottino (1947) for the formation of the Etruscan ethnos inhibited the undertaking of new research on the relationship between the Etruscans and the Near East, especially in the context of the sacred. However, recent studies have suggested that elements like lituus, the curved stick used by the augur, and the practice of the haruspicy derived from Mesopotamia or from the Anatolian area. It has been suggested that this can be explained by the immigration of people who moved from Anatolia to Italy at the end of the Bronze Age (Bachvarova 2012). An oriental influence can possibly be recognised in the foundation rituals of the Etruscan cities, where the archeological evidence suggests the involvement of Vei, a female chthonic divinity, and Aplu-Rath-Śuri-Manth, an oracular and infernal god. Vei and Aplu-Rath-Śuri-Manth evoke the two Greek gods, Demeter and Apollo, who in turn have elements in common with the Hittite founder and agrarian god Telepinu. In this paper I shall discuss the oriental influence on Etruscan religion by critically reviewing the hypotheses that suggest that some of its elements originated in the Near East. By considering the Etruscans’ religious behavior I will argue that religious data can help us to investigate the process of ethnic formation in the past and to reconstruct the external stimuli, events and movements of people that may have contributed to it.

Il dialogo della Comunità di Gesù con la Chiesa Ortodossa Rumena.

"Tempi di Unità" - Periodico della Comunità di Gesù - Quadrimestrale, Giugno-Dicembre 2007.

The Oriental Institute Annual Report, 2017

9 IN MEMORIAM 13 PROJECT REPORTS 15 ÇADIR HÖYÜK. Gregory McMahon 23 CHICAGO DEMOTIC DICTIONARY (CDD). Brian Muhs and Janet H. Johnson 25 CHICAGO HITTITE DICTIONARY (CHD). Theo van den Hout 29 COPING WITH CHANGING CLIMATES IN EARLY ANTIQUITY (3CEA). Hervé Reculeau and Nadine Moeller 33 DENDARA. Grégory Marouard and Nadine Moeller 35 EPIGRAPHIC SURVEY. W. Raymond Johnson 55 EXCAVATIONS AT SUREZHA. Gil J. Stein 71 GALILEE PREHISTORY PROJECT.