Papers by Juan Carlos Noa-Carrazana
Frontiers in Plant Science, Oct 22, 2021
Coffea spp. are tropical plants used for brewing beverages from roasted and grounded seeds, the f... more Coffea spp. are tropical plants used for brewing beverages from roasted and grounded seeds, the favorite drink in the world. It is the most important commercial crop plant and the second most valuable international commodity after oil. Global coffee trade relies on two Coffea species: C. arabica L. (arabica coffee) comprising 60% and C. canephora (robusta) comprising the remaining 40%. Arabica coffee has lower productivity and better market price than robusta. Arabica coffee is threatened by disease (i.e., coffee leaf rust), pests [i.e., Hypothenemus hampei or coffee berry borer (CBB) and nematodes], and susceptibility to climate change (i.e., drought and aluminum toxicity). Plant biotechnology by means of tissue culture inducing somatic embryogenesis (SE) process, genetic transformation, and genome editing are tools that can help to solve, at least partially, these problems. This work is the continuation of a protocol developed for stable genetic transformation and successful plant regeneration of arabica coffee trees expressing the Bacillus thuringiensis (Bt) toxin Cry10Aa to induce CBB resistance. A highly SE line with a high rate of cell division and conversion to plants with 8-month plant regeneration period was produced. To validate this capability, gene expression analysis of master regulators of SE, such as BABY BOOM (BBM), FUS3, and LEC1, embryo development, such as EMB2757, and cell cycle progression, such as ETG1 and MCM4, were analyzed during induction and propagation of non-competent and highly competent embryogenic lines. The particle bombardment technique was used to generate stable transgenic lines after 3 months under selection using hygromycin as selectable marker, and 1 month in plant regeneration. Transgenic trees developed fruits after 2 years and demonstrated expression of the Bt toxin ranging from 3.25 to 13.88 µg/g fresh tissue. Bioassays with transgenic fruits on CBB first instar larvae and adults induced mortalities between 85 and 100% after 10 days. In addition, transgenic fruits showed a seed damage lower than 9% compared to 100% of control fruits and adult mortality. This is the first report on stable transformation and expression of the Cry10Aa protein in coffee plants with the potential to control CBB.
Cuadernos de fitopatología: Revista técnica de fitopatología y entomología, 1995
ABSTRACT
Revista de la Facultad de Agronomía, Universidad del Zulia, 2021
There are reports of Diaphorina citri resistance in the state of Veracruz to chlorpyrifos and dim... more There are reports of Diaphorina citri resistance in the state of Veracruz to chlorpyrifos and dimethoate due to the excessive use of these products for the control of pests, among them D. citri. The purpose of this work was to diagnose the susceptibility of D. citri adults to chlorpyrifos and dimethoate in commercial citrus orchards of the municipality of Martinez de la Torre, Veracruz. For this, 200 adults of D. citri were collected in each of 30 orchards, and topical application bioassays were performed with dimethoate at 0.0090% concentration and chlorpyrifos at 0.0085% concentration. Diaphorina citri adults without chemical control during 10 years were used as a control. Mortality of D. citri with dimethoate and chlorpyrifos averaged 85%, in contrast the control mortality was 99% and 98.8% with dimethoate and chlorpyrifos, respectively. According to these results, it can be inferred that D. citri has some resistance to organophosphate insecticides in the orchards of Martinez de ...
Tropical and Subtropical Agroecosystems, Apr 7, 2022
Background: The Potyvirus Papaya ringspot virus type P (PRSV-p) causes the papaya (Carica papaya ... more Background: The Potyvirus Papaya ringspot virus type P (PRSV-p) causes the papaya (Carica papaya L.) ringspot disease, which causes damage to the plantations of this crop. In regions where there is movement of plant material and continuous production processes, there is greater genetic variability of this virus; such is the case of the State of Veracruz, Mexico, one of the main papaya producers in the country. Objective: To analyze the genetic variability of isolates of PRSV-p, present in commercial papaya plantations in the central zone of the State of Veracruz. Methodology: Leaves with differential symptoms of the disease were collected. The capsid protein (CP) coding region was amplified by RT-PCR to confirm the identity of the virus. The sequences obtained were analyzed, aligned and a phylogenetic tree was obtained. Results: The presence of PRSV-p was confirmed by RT-PCR. Through sequence analysis, high diversity was observed in the first 50 amino acids encoded in the N-terminal region of CP. In the phylogenetic tree, three viral genetic groups were obtained. The largest conserved sites of interest (even the WCIEN box and QMKAAA) were found near the C-terminal region of the CP. 100% homology was observed in the central and C-terminal region of the Ver75Co isolate, with respect to the consensus sequence. Implications: The results show the genetic diversity of the virus in the central zone of the state of Veracruz, which will allow genetic modifications to improve resistance in papaya plants. Conclusions: There is genetic variability of PRSV-p in Veracruz, there are conserved sites of interest (WCIEN and QMKAAA) and changes in the N-terminal region, which includes the EK repetitive region and the DAG codon. These changes may be a genetic characteristic of the virus for adaptation. The Ver75Co isolate may be the point of change that gives rise to the other isolates of this work. The phylogenetic tree shows a single genetic group of the virus, which is distributed in three different clades.
Journal of General and Molecular Virology, Dec 31, 2009
An unknown symptomatology, including yellowing in old plantations, colorless of cladode epidermis... more An unknown symptomatology, including yellowing in old plantations, colorless of cladode epidermis, cladode deforming and curly, were observed on the Indian Fig (prickly pear cactus) (Opuntia ficus-indica Mill) in the “Pyramids” Indian area located in the northeastern State of Mexico. The samples collected were analyzed through a nested-PCR reaction. The symptoms found, were all attributed to the presence of a phytoplasma given the amplification of a 1200 pb fragment of the 16S rRNA gene using primers R16 F2/R2. The PCR products restriction analysis leading us to assume that the phytoplasma corresponds to subgroup 16Srll, previously reported for other crops in other world regions. Key words: Indian fig, phytoplasma, Mexico state, 16S rRNA, PCR detection.
La estacionalidad reproductiva es una característica de la mayoría de las razas de ovejas y cabra... more La estacionalidad reproductiva es una característica de la mayoría de las razas de ovejas y cabras de latitudes templadas y subtropicales (
Acta Physiologiae Plantarum, Mar 1, 2019
Fusarium oxysporum f. sp. vanillae (Fov), the most devastating pathogen of Vanilla planifolia, an... more Fusarium oxysporum f. sp. vanillae (Fov), the most devastating pathogen of Vanilla planifolia, an important orchid used in the food industry, causes stem and root rot. Vanilla genotypes that are resistant to Fov currently do not exist, so vanilla plants that are resistant to this fungus are needed. In vitro selection offers an effective means to accomplish this objective. Resistant shoots were selected on Murashige and Skoog medium (MS) containing 8.88 µM of 6-benzyladenine and various concentrations of Fov culture filtrate (30, 40 and 50%, v/v). The control treatment (0%) was exclusively MS medium without any culture filtrate. After two 60-day selection cycles, 40 resistant shoots were obtained. In vitro, about 35% of the shoots were resistant to 50% Fov culture filtrate, while in vivo tests indicated that 26.6% of the plants had acquired resistance to the pathogen under greenhouse conditions after 9 weeks. The protocol employed in this study, which forms part of a wider genetic improvement program for this orchid, allowed Fov-resistant V. planifolia plants to be obtained within 420 days.
Applied Soil Ecology, Aug 1, 2013
ABSTRACT The endogeic earthworm Pontoscolex corethrurus (Müller, 1857) was the most abundant spec... more ABSTRACT The endogeic earthworm Pontoscolex corethrurus (Müller, 1857) was the most abundant species (75%) insoil contaminated with hydrocarbons, mostly benzo(a)pyrene (BaP), in the state of Tabasco (Mexico). Theearthworm P. corethrurus was tested for its capacity to remove 100 mg BaP kg−1from an Anthrosol soil(sterilized or not) and amended with legume Mucuna pruriens (L.) DC. var. utilis (Wall. ex Wight) Baker exBurck (3%) or the grass Brachiaria humidicola (L.) DC (3%) (recently renamed as Urochloa humidicola (Ren-dle) Morrone & Zuloaga) in an aerobic incubation experiment. P. corethrurus removed 26.6 mg BaP kg−1from the sterilized soil and application of B. humidicola as feed increased this to 35.7 mg BaP kg−1and M.pruriens to 34.2 mg BaP kg−1after 112 days. The autochthonous microorganisms removed 9.1 mg BaP kg−1from the unsterilized soil and application of B. humidicola increased this to 18.0 mg BaP kg−1and M.pruriens to 11.2 mg BaP kg−1. Adding P. corethrurus to the unsterilized soil accelerated the removal of BaPand 36.1 mg kg−1was dissipated from soil. It was found that the autochthonous microorganisms removedBaP from soil, but addition of P. corethrurus increased the dissipation 4-fold. The endogeic earthworm P.corethrurus can thus be used to remediate hydrocarbon-contaminated soils in tropical regions.
Revista mexicana de fitopatología, Apr 17, 2020
Resumen. El Papaya ringspot virus (PRSV-P) produce diferentes síntomas, los que podrían variar co... more Resumen. El Papaya ringspot virus (PRSV-P) produce diferentes síntomas, los que podrían variar con las condiciones ambientales y con la severidad. El objetivo fue caracterizar la diversidad de síntomas mediante gráficos radiales y analizar la evidencia patogénica como soporte de la variabilidad poblacional de PRSV-P presente en plantaciones comerciales de papayo var. Maradol roja en la zona Centro del estado de Veracruz. Se revisaron visualmente los síntomas prevalentes en ≈200 plantas por parcela. Se colectaron hojas que conformaron seis grupos diferenciales de síntomas. Por cada grupo se
Bananas are the developing world's fourth most important food crop. Many cultivated clones ar... more Bananas are the developing world's fourth most important food crop. Many cultivated clones are hybrids between M. acuminata (A genome) and M. balbisiana (B genome). Recently, banana streak disease infection has been causing serious yield loses. The disease is caused by banana streak badnavirus (BSV) and the infections seem to result from the activation of retroviral sequences integrated into the B genome. In order to facilitate positional cloning, physical mapping and genome sequencing, we have constructed a BAC library from M. balbisiana. We have isolated high-molecular-weight genomic DNA using a standard and novel protocols, the latter involving flow cytometry to purify nuclei from tissue homogenates. The library was constructed by partial digestion of genomic DNA with HindIII restriction enzyme and cloning into the pIndigoBAC-5 vector. The average insert size of the library is 135 kb with more than 70% of inserts larger than 120 kb. The entire library consists of 36,864 clones and represents 9x genome equivalents. Screening with chromosome-specific markers proved representation of the whole B genome and confirmed estimated genomic coverage. On average, the library is contaminated with 3% chloroplast clones and 0.004% mitochondrial clones. However, only 0.09% clones obtained from flow-sorted nuclei contain chloroplast DNA, indicating the advantage of the novel procedure. This library provides an essential resource for the analysis of BSV activation mechanisms and isolation of genes responsible for resistance to biotic and abiotic stress. The project was supported by CIRAD, Academy of Sciences of the Czech Republic (IAA6038201) and French Ministry of Foreign Affairs (COCOP 17/01). (Texte integral)
Revista de la Facultad de Ciencias Agrarias UNCuyo
This research determined the degree of transition towards organic production and management of Va... more This research determined the degree of transition towards organic production and management of Valencia orange in the municipalities of northern Veracruz State. One hundred and six surveys were administered to producers grouped into four consolidated civil associations. A constructed transition index considering six variables revealed an average of 0.768. Citrus grower/institution positively correlated with intercropping (p = 0.0281) and with internal factors (p = 0.0257). Determinant factors for transition index were intercropping (p = 0.00009), and citrus grower/institution interaction (p = 0.0000). These internal and external factors together with years of conversion, averaging six years, resulted in an intermediate transition degree towards organic Valencia orange production. Municipalities of northern Veracruz State stand on varying degrees of transition towards organic agriculture. Yet, many growers still conserve some management practices not consistent with organic productio...
Revista Mexicana de Fitopatología, Mexican Journal of Phytopathology
Dieback disease caused by fungal complexes is a severe problem in mango trees (Mangifera indica).... more Dieback disease caused by fungal complexes is a severe problem in mango trees (Mangifera indica). Its main symptoms are branch rot, gummosis, and finally, the tree’s death. In this work, the species of the fungal complex causing mango dieback in the Manila variety in Veracruz, Mexico were identified. The in vitro interaction of two species belonging to the complex was evaluated and the severity of the co-infection in mango branches. Lasiodiplodia pseudotheobromae and Pestalotiopsis mangiferae were identified as causal agents of mango dieback in the producing area of Veracruz. In coinfected mango branches, greater severity of necrosis was observed than in individual infections. Liquid culture filtrates applied in co-cultures showed different results for each species of phytopathogen. The P. mangiferae filtrate had no significant antagonistic effects on the growth of L. pseudotheobromae (inhibition of 2.68%), while the L. pseudotheobromae filtrate inhibited 41.38% of P. mangiferae. Th...
Fig. 1. Morphological characteristics of Dendroctonus approximatus. a) Lateral and dorsal views, ... more Fig. 1. Morphological characteristics of Dendroctonus approximatus. a) Lateral and dorsal views, b) Morphometry of face and elytral declivity, c) Dorsal and lateral views of aedeagus and spicule.
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Papers by Juan Carlos Noa-Carrazana
Magnolia schíedeana son especies endémicas de México, el cambio climático, la destrucción del hábitat debido a la expansión de las actividades productivas como la agricultura, la ganadería, la· extracción forestal, incendios forestales, deterioro de cuencas hidrológicas, erosión de los suelos introducción de especies de flora y fauna exótica, trafico de especies, desarrollo urbano y su limitada distribución natural, son las principales causas que hacen que éstas especies se encuentre en peligro de extinción, quedando recluidas sólo en zonas donde el ambiente es parecido (Cartujano et al., 2002, A\anís et al.,2004; Cicuzza et al., 2007; Corral-Aguirre y Sánchez-Velásquez, 2006; Sánchez y Pineda, 2006).