Papers by Patrícia Mazureki Campos
Research, Society and Development
This study proposes to develop a biomonitorated study of the Euphorbia umbellata leaves extracts ... more This study proposes to develop a biomonitorated study of the Euphorbia umbellata leaves extracts to identify those that could be used to prepare, by green synthesis, silver nanoparticles (AgNPs), with cytotoxic and antimicrobial potential. Hexane, dichloromethane, acetone, methanol, and aqueous extracts were evaluated to measure the terpenes amounts, antimicrobial activity (Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus), and the cytotoxic potential (A549, H292 cell lines). Characterization of AgNPs was performed by determination of zeta potential, polydispersity index, mean diameter, UV/Vis spectrometry, and scanning electron microscope (SEM), and evaluated against cytotoxic and antimicrobial models. Hexane and dichloromethane extracts presented the higher amounts of terpenes, exhibited microbiological inhibition against K. pneumoniae and S. aureus, and cytotoxic potential against both cell lines. AgNPs prepared with hexane and dichloromethane extracts were de...
African journal of pharmacy and pharmacology
In traditional medicine, a variety of plants have been used as sexual stimulants. Within the genu... more In traditional medicine, a variety of plants have been used as sexual stimulants. Within the genus Tynanthus (Bignoniaceae) some species are known by their aphrodisiac effects, including Tynanthus micranthus Corr. & Mello ex. Schum. The purpose of this study was to evaluate the sexual behavior of male mice treated with a crude extract of the aerial parts (leaves and stems) of T. micranthus, for this, the extracts were prepared and orally administered (5 and 10 mg/kg) to different groups of male Swiss mice. Mating behavior was filmed and compared with control (distilled water) and test drug (sildenafil citrate), 1 and 3 h after treatment. The animals treated with the extract presented a significant reduction in the latency time for mounts and an increased frequency of mounts, especially after three hours of treatment, in a dose-related manner, as compared to the control group. These results prove the influence of T. micranthus on sexual behavior of mice and allow the inclusion of thi...
Journal of Ethnopharmacology, 2013
Ethnopharmacology relevance: Garcinia gardneriana (Planchon and Triana) Zappi (Clusiaceae) is pop... more Ethnopharmacology relevance: Garcinia gardneriana (Planchon and Triana) Zappi (Clusiaceae) is popularly called "bacopari" in southern Brazil. The leaves of this plant are traditionally used to treat skin disorders. Aim of study: This study evaluated the effects of a hydroalcoholic extract of Garcinia gardneriana leaves (HEGG) on B16F10 murine melanoma cells in order to search for new depigmenting agents. Materials and methods: The effects of HEGG were assessed in melanin content assays in B16F10 melanoma cells compared with the reference drug kojic acid (500 mM). Melanin content was measured after spontaneous melanogenesis, UVB-induced melanogenesis and melanogenesis induced by α-MSH. At the same time, cell viability assays were conducted. Intracellular and mushroom tyrosinase activity assays were employed to evaluate the effect of HEGG on tyrosinase activity. Results: HEGG decreased the level of melanin under all three experimental conditions of melanin content evaluation without reducing cell viability. In intracellular tyrosinase assays, the enzyme's activity was reduced about 19% with extract concentrations ranging 0.1-10 mg/mL. In the mushroom tyrosinase activity assay a maximal inhibition of 35% (1000 mg/mL) was observed. Conclusion: These results suggest that HEGG inhibition relates to its tyrosinase activity. Therefore, the hydroalcoholic extract of Garcinia gardneriana shows great potential for use as a depigmenting agent in hyperpigmentation disorders.
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Papers by Patrícia Mazureki Campos