Papers by Feky R . Mantiri
Plant Physiology, 2008
Transcriptional profiling of embryogenic callus produced from Medicago truncatula mesophyll proto... more Transcriptional profiling of embryogenic callus produced from Medicago truncatula mesophyll protoplasts indicated up-regulation of ethylene biosynthesis and ethylene response genes. Using inhibitors of ethylene biosynthesis and perception, it was shown that ethylene was necessary for somatic embryogenesis (SE) in this model legume. We chose several genes involved in ethylene biosynthesis and response for subsequent molecular analyses. One of these genes is a gene encoding a transcription factor that belongs to the AP2/ERF superfamily and ERF subfamily of transcription factors. We demonstrate that this gene, designated M. truncatula SOMATIC EMBRYO RELATED FACTOR1 (MtSERF1), is induced by ethylene and is expressed in embryogenic calli. MtSERF1 is strongly expressed in the globular somatic embryo and there is high expression in a small group of cells in the developing shoot meristem of the heart-stage embryo. RNA interference knockdown of this gene causes strong inhibition of SE. We al...
Plant Developmental Biology - Biotechnological Perspectives, 2010
AIP Conference Proceedings, 2023
IOP Conference Series: Earth and Environmental Science, 2019
Information of thermophilic bacteria in Indonesian hot springs is limited, especially in the prov... more Information of thermophilic bacteria in Indonesian hot springs is limited, especially in the province of North Sulawesi. The study aims to isolate and identify thermophilic bacteria from Remboken hot springs at Minahasa Indonesia, identify the α-amylase gene and its enzyme activity. To identify the thermophilic bacteria, it was conducted the morphological analysis and biochemistry tests. Th microbial species was identified using PCR and sequencing of 16S rRNA followed by BLAST search on the EZBioCloud database, whereas the α-amylase gene was identified by Polymerase chain reaction (PCR). Starch hydrolysis test was used to analyze αamylase activity qualitatively. It was done using iodine method (Fuwa method). The results showed that thermophilic bacteria isolated belong to the species Ureibacillus suwonensis, Anoxybacillus thermarum, and Anoxybacillus mongoliensis. PCR analysis showed that Anoxybacillus thermarum FRM-RBK02 possesses an α-amylase gene. The strain was able to hydrolyze amylose, indicated by a clear zone around the growth; hence exhibiting α-amylase activity. The the crude amylase of Anoxybacillus thermarum FRM-RBK02 exhibited optimum activity at 80°C and pH 7.0. Overall, Anoxybacillus thermarum FRM-RBK02 has α-amylase gene and could produce thermostable α-amylase with amylolytic capability.
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Papers by Feky R . Mantiri