The lipid composition of five human breast cancer cell lines (MCF-7, T47D, ZR-75-1, SKBR3 and MDA... more The lipid composition of five human breast cancer cell lines (MCF-7, T47D, ZR-75-1, SKBR3 and MDA-MB231) was assessed by proton magnetic resonance spectroscopy (MRS) in whole cells and membraneenriched fractions. The proportions of the three main lipid resonances in 1D spectra were different for each cell line. These resonances included mobile methyl and methylene functions from fatty acids of triglycerides and phospholipids and N-trimethyl from choline of phospholipids. T47D and ZR-75-1 cells presented a high methylene/methyl ratio (6.02 ± 0.35 and 6.28 ± 0.90). This ratio was significantly lower for SKBR3, MCF-7 and MDA-MB231 cells (2.76 ± 0.22, 2.27 + 0.57 and 1.39 ± 0.39). The N-trimethyl/methyl ratio was high for MDA-MB231 and SKBR3 cells (1.38 ± 0.54 and 0.86 ± 0.32), but lower for MCF-7, T47D and ZR-75-1 cells (0.49 ± 0.11, 0.16 ± 0.07 and 0.07 ± 0.03). 2D COSY spectra confirmed these different proportions in mobile lipids. From ID spectra obtained on membrane preparations, T47D and ZR-75-1 were the only cell lines to retain a signal from mobile methylene functions. These differences might be related to the heterogeneity found for several parameters of these cells (tumorigenicity, growth rate, hormone receptors); an extended number of cases from fresh samples might enable clinical correlations.
Prosthetic graft infection remains one of the most dreaded complications of reconstructive vascul... more Prosthetic graft infection remains one of the most dreaded complications of reconstructive vascular surgery, especially when the aorta is involved. Despite optimal surgical technique and appropriate systemic antibiotic prophylaxis, the incidence of graft infection persists to range between 1.0% and 6.0%. 1-3 Although continuous improvement in results has been achieved over the past decade, prosthetic graft infection is still associated with high mortality and amputation rates. 4-6 Dissatisfaction with extraanatomic techniques has led to the development of approaches of in situ replacement by
American Journal of Physiology-renal Physiology, Jul 1, 2008
JM. A p38 mitogen-activated protein kinase inhibitor protects against renal damage in a non-heart... more JM. A p38 mitogen-activated protein kinase inhibitor protects against renal damage in a non-heart-beating donor model.
American Journal of Physiology-regulatory Integrative and Comparative Physiology, Jun 1, 2005
Survival and some physiological responses to freezing were investigated in three European water f... more Survival and some physiological responses to freezing were investigated in three European water frogs (Rana lessonae, Rana ridibunda, and their hybridogen Rana esculenta). The three species exhibited different survival times during freezing (from 10 h for R. lessonae to 20 h for R. ridibunda). The time courses of percent water frozen were similar; however, because of the huge differences in body mass among species (from 10 g for Rana lessonae to nearly 100 g for Rana ridibunda), the ice mass accumulation rate varied markedly (from 0.75 Ϯ 0.12 to 1.43 Ϯ 0.11 g ice/h, respectively) and was lowest in the terrestrial hibernator Rana lessonae. The hybrid Rana esculenta exhibited an intermediate response between the two parental species; furthermore, within-species correlation existed between body mass and ice mass accumulation rates, suggesting the occurrence of subpopulations in this species (0.84 Ϯ 0.08 g ice/h for small R. esculenta and 1.78 Ϯ 0.09 g ice/h for large ones). Biochemical analyses showed accumulation of blood glucose and lactate, liver glucose (originating from glycogen), and liver alanine in Rana lessonae and Rana esculenta but not in Rana ridibunda in response to freezing. The variation of freeze tolerance between these three closely related species could bring understanding to the physiological processes involved in the evolution of freeze tolerance in vertebrates. Address for reprint requests and other correspondence: Y. Voituron, Physiologie des régulations énergétiques, cellulaires et moléculaires (U.M.R.
Biochimica Et Biophysica Acta - General Subjects, Aug 1, 2004
In organ transplantation, preservation injury is an important factor which could influence short-... more In organ transplantation, preservation injury is an important factor which could influence short-term and long-term graft outcome. The renal medulla is particularly sensitive to oxidant stress and ischemia-reperfusion injury (IRI). Using an autotransplant pig kidney model, we investigated renal function and medullary damage determined between day 1 and week 2 after 24-or 48-h cold storage in different preservation solutions: University of Wisconsin solution (UW), Hopital Edouard Herriot solution (a high Na + version of UW), ECPEG (high Na + preservation solution with PEG) and ICPEG (a high K + version of ECPEG) with or without trimetazidine (TMZ). TMZ improved renal preservation and increased renal function when added in each preservation solution (particularly HEH and ECPEG). Medullary damage led to the early appearance of trimethylamine-N-oxide (TMAO) followed by 1 H-NMR in urine and plasma. TMZ and ECPEG is the most efficient association to reduce medullary damage. This study clarifies the role of colloid and polarity solution and the role of mitochondrial protection by TMZ.
The aim of the study was to test a new preservation solution containing polyethylene glycol (S.C.... more The aim of the study was to test a new preservation solution containing polyethylene glycol (S.C.O.T. solution) as pancreatic islet isolation medium both to increase the islet yield and to prolong the allograft survival. In a model of islet transplantation in diabetic mouse, islets were isolated with S.C.O.T. in experimental groups and with Hank's balanced salt solution (HBSS) in control groups. The use of S.C.O.T. solution improved the islet yield (596+/-27 IEQ/pancreas) as compared to HBSS (456+/-11 IEQ/pancreas) (P<0.001). Allograft survival was prolonged in experimental group (17.3+/-4.3 days) versus controls (7.3+/-3.6 days) in a full mismatch combination (P<0.001) and in absence of recipient immunosuppression. The same prolongation (10 days) was also found in a strongly alloreactive transgenic combination. It is hypothesized that a transitory phenomenon of immunocamouflage of the graft surface antigens occurs, as shown by immunofluorescence studies. The use of this new solution could improve the results of islet transplantation in humans.
Journal of the American College of Cardiology, Jul 1, 1988
Although the introduction of cyclosporine has markedly improved survival in heart transplant reci... more Although the introduction of cyclosporine has markedly improved survival in heart transplant recipients, early detection of cardiac allogmtl rejection remains the major problem to be solved. Hitherto, the diagnosis of cardiac rejection has teen based on the results of cndomyocardial biopsy. which is considered as the only reliable test. However, ideally the detection of cardiac rejection should be noninvasive to allow frequent follow-up of the patients. Among the noninvasive techniques, neither the clectmcardiogram (I) nor the M-m& echccardiogram (2) is a reliable method for detection of acute cardiac rejection, especially in patients treated with cyclosporinc. Reports have shown yod correlation between h4hu io ha&e val8es altw bMluasu~ve therapy 4 < 0.05 and B < 0.0005. i-at~~tivel~). With 20% de. indexes of left ventricular filling obtained by Doppler echo. cardiography and those obtained by contrast angiography (3) or mdionuclide angiogmphy (4). Recently, analysis ofthc left ventriculardiastoli~ nmction with Doppler echacardiogsaphy has been shown (5) to be a useful and readily repeatable technique for the surveillance of cardiac recipients. Accordingly, we assessed the value of Doppler echocardiogmphy for detecting allograft cardiac rejection in 55 cardiac transplant recipients. Methods Study patieals. Fifty-five patients, who were examined by pulsed Doppler echocardiography and endomyocardial biopsy within 2 months after orthotopic cardiac ttansplantation were included in this study. Three subsas of p&ems were excluded from the srudy: IO patients (14%) with severe rejection associated with a major impairment of kn ventricular systolic function evaluated by echccardiographic imaging: 2 patients (3%) with brought to you by CORE View metadata, citation and similar papers at core.ac.uk
Analytical and Bioanalytical Chemistry, Nov 27, 2009
Metabolomics is a comprehensive method for metabolite assessment that involves measuring the over... more Metabolomics is a comprehensive method for metabolite assessment that involves measuring the overall metabolic signature of biological samples. We used this approach to investigate biochemical changes due to acute and chronic physical exercise. Twenty-two women using identical oral contraceptives were segregated into an untrained (n=10) or trained (n=12) group depending on their physical training background. The subjects performed two exercises in a randomized order: a prolonged exercise test (75% of their V Á O 2 max until exhaustion) and a short-term, intensive exercise test (short-term, intensive exercise anaerobic test). Urine specimens were collected before and 30 min after each test. The samples were analyzed by 1 H NMR spectroscopy, and multivariate statistical techniques were utilized to process the data. Distinguishing characteristics were observed only in the urine profiles of specimens collected before vs. 30 min after the short-term, intensive exercise test. The metabolites responsible for such changes were creatinine, lactate, pyruvate, alanine, β-hydroxybutyrate, acetate, and hypoxanthine. In both groups, the excretion of lactate, pyruvate, alanine, β-hydroxybutyrate, and hypoxanthine increased similarly after the completion of the short-term, intensive exercise test (p<0.03). However, acetate excretion increased to a lesser extent in trained than in untrained subjects (p< 0.05). In conclusion, metabolomics is a promising tool in order to gain insight into physiological status and to clarify the changes induced by short-term, intense physical exercise.
American Journal of Obstetrics and Gynecology, Feb 1, 1994
OBJECTIVES: To precisely evaluate fetal renal function in cases of bilateral fetal urinary tract ... more OBJECTIVES: To precisely evaluate fetal renal function in cases of bilateral fetal urinary tract obstruction, we analyzed fetal urine metabolites by nuclear magnetic resonance spectroscopy. STUDY DESIGN: Fifty-six specimens of fetal urine samples In utero were retrospectively analyzed by proton nuclear magnetic resonance. Group 1 (n = 21) consisted of survivors for a1 year with normal serum creatinine (<50 µmol/L), for group 2 (n = 17) of survivors with abnormal serum creatinine (>50 pmol/L), and group 3 (n = 18) of those with bilateral histologic dysplasla associated with fetal (termination of pregnancy) or neonatal death. RESULTS: A specific nuclear magnetic resonance profile was obtained for each of the three groups of renal function. A two-dimensional representation of alanine-valine and valine-threonine concentrations allows clear differentiation between the three degrees of renal function, with a sensitivity and specificity of 88% and 86%, respectively. CONCLUSIONS: In fetal bilateral urinary tract obstruction a fetal urine nuclear magnetic resonance profile allows an accurate identification of renal function at birth.
Citrate (Cit) is an organic acid normally found in urine and a central component of the tricarbox... more Citrate (Cit) is an organic acid normally found in urine and a central component of the tricarboxylic acid cycle. Changes in renal metabolism have been proposed to cause dramatic changes in Cit excretion with acid-base imbalances. Cold ischemia stimulates glycolysis and glycogenolysis and increases the production of lactic acid and the concentration of hydrogen ions with tissue acidification.
Abnormal movement of the mitral valve observed in patients with hypertrophic obstructive cardiomy... more Abnormal movement of the mitral valve observed in patients with hypertrophic obstructive cardiomyopathy (HOCM) consists of variable degrees of systolic anterior motion (SAM) towards the interventricular septum and has been recognized by angiography and echocardiography. Several explanations of its mechanism have been suggested: abnormal insertion of the anterior mitral leaflet (1), malposition of the papillary muscles (2), posterior displacement of the mitral annulus by septal hypertrophy (3) and venturi effect due to the high speed of ejection in the left ventricular outflow tract (4). A close correlation between SAM and the intraventricular pressure gradient has been demonstrated and SAM is also thought to play a part in the mechanism of mitral incompetence, the incidence of which increases with the severity of the disease (5).
Cellular and molecular biology (Noisy-le-Grand, France), 2004
In allogenic transplant the immediate immune response is due to the recipient T cell recognition ... more In allogenic transplant the immediate immune response is due to the recipient T cell recognition of non-self molecules presented on graft resident donor antigen presenting cells. An alternative to the transplantation tolerance paradigm is based on the development of strategies which distort alloimmune recognition of the graft by antigen reactive cells of the recipient. Immunocamouflage relies on the modification of the cell membrane surface with non-immunogenic molecules creating a barrier that prevents the recognition of antigenic sites by cells and antibodies of the recipient. Polymers can spontaneously bind to cell and tissues surfaces and sterically stabilize the underlying surface from interactions with other components in the surrounding. They can be adsorbed or chemically grafted to surfaces. Polyethylene glycol (PEG) seems to be the more effective at sterically stabilizing underlying surfaces. The outstanding protection provided by this polymer has been attributed to its mol...
... Sonia Borra, MD, David Hawkins, MD, FRCP (C), William Duguid, MB, B.CH., MRC (Path.), and Mic... more ... Sonia Borra, MD, David Hawkins, MD, FRCP (C), William Duguid, MB, B.CH., MRC (Path.), and Michael Kaye, MB, FRCP (C). ... On January 30, the total white-cell count was 5600, with 15 per cent eosinophils. ... 2. Schreiner GE, Maher JF: Toxic nephropathy . ...
Multidrug resistance (MDR) confers resistance to anticancer drugs and reduces therapeutic efficie... more Multidrug resistance (MDR) confers resistance to anticancer drugs and reduces therapeutic efficiency. It is often characterized by the expression of the MDR1 gene product P-glycoprotein (or gp170) at the membrane of tumor cells. To further propose a potential complementary tool in cancer treatment, the sensitivity of gp170 tumor cells to NK-dependent lysis was investigated. Two kinds of cells were generated from wild-type K562 erythroleukemic cells: the first were derived from Taxol-selected cells and cloned, whereas the second were retrovirally transduced by the cDNA of the MDR1 gene. The last process was also applied to the human embryonal carcinoma cells called Tera-2 cells. First, both cloned and MDR-1 K562 cells appeared highly susceptible to naive NK cell killing. Interestingly, in addition, Tera-2 cells that were not sensitive to NK lysis could be killed when they expressed gp170 at their membranes. In previous data, we demonstrated that NK cell release of bimolecular complex...
Transplant international : official journal of the European Society for Organ Transplantation, 2015
Introduction: Ischemia reperfusion (IR) injury is unavoidable during organ transplantation and le... more Introduction: Ischemia reperfusion (IR) injury is unavoidable during organ transplantation and leads to complications. With the increased use of marginal donors, more sensitive to IR, solutions must be found to improve outcome. We hypothesized that a higher preservation temperature could offer better protection against IR.[br/]Methods: We tested this in an in vitro model of IR using primary endothelial cells and in ex vivo preserved pig kidneys. In both, 24 h preservation in University of Wisconsin solution was used.[br/]Results: In vitro, compared to 4°C, temperatures between 19 and 32°C provided higher protection against cell death (LDH release test), permitting better mitochondrial function (complexes II and V activity tests) and a lowerexpression of endothelial activation and inflammation markers TLR4, MCP1 and ICAM1. Ex vivo, however, the superiority of 19 or 32°C was lost, as preserved pig kidneys showed similar levels of tissue damage (both tubulardilatation, loss of brush border and endoluminal detachment) during preservation, and at 24 h the 4°C kidneys displayed a trend towards less damage. In addition, the tissular Monocyte/Macrophage infiltration was increased in the 19°C and more in the 32°C temperature conservation as compared to 4°C storage.[br/]Conclusion: Our study shows that although a higher preservation temperature is preferable for cell survival and function, whole organ testing demonstrates that conceptual work needs to be performed to harness the potential of sub-normothermia
The lipid composition of five human breast cancer cell lines (MCF-7, T47D, ZR-75-1, SKBR3 and MDA... more The lipid composition of five human breast cancer cell lines (MCF-7, T47D, ZR-75-1, SKBR3 and MDA-MB231) was assessed by proton magnetic resonance spectroscopy (MRS) in whole cells and membraneenriched fractions. The proportions of the three main lipid resonances in 1D spectra were different for each cell line. These resonances included mobile methyl and methylene functions from fatty acids of triglycerides and phospholipids and N-trimethyl from choline of phospholipids. T47D and ZR-75-1 cells presented a high methylene/methyl ratio (6.02 ± 0.35 and 6.28 ± 0.90). This ratio was significantly lower for SKBR3, MCF-7 and MDA-MB231 cells (2.76 ± 0.22, 2.27 + 0.57 and 1.39 ± 0.39). The N-trimethyl/methyl ratio was high for MDA-MB231 and SKBR3 cells (1.38 ± 0.54 and 0.86 ± 0.32), but lower for MCF-7, T47D and ZR-75-1 cells (0.49 ± 0.11, 0.16 ± 0.07 and 0.07 ± 0.03). 2D COSY spectra confirmed these different proportions in mobile lipids. From ID spectra obtained on membrane preparations, T47D and ZR-75-1 were the only cell lines to retain a signal from mobile methylene functions. These differences might be related to the heterogeneity found for several parameters of these cells (tumorigenicity, growth rate, hormone receptors); an extended number of cases from fresh samples might enable clinical correlations.
Prosthetic graft infection remains one of the most dreaded complications of reconstructive vascul... more Prosthetic graft infection remains one of the most dreaded complications of reconstructive vascular surgery, especially when the aorta is involved. Despite optimal surgical technique and appropriate systemic antibiotic prophylaxis, the incidence of graft infection persists to range between 1.0% and 6.0%. 1-3 Although continuous improvement in results has been achieved over the past decade, prosthetic graft infection is still associated with high mortality and amputation rates. 4-6 Dissatisfaction with extraanatomic techniques has led to the development of approaches of in situ replacement by
American Journal of Physiology-renal Physiology, Jul 1, 2008
JM. A p38 mitogen-activated protein kinase inhibitor protects against renal damage in a non-heart... more JM. A p38 mitogen-activated protein kinase inhibitor protects against renal damage in a non-heart-beating donor model.
American Journal of Physiology-regulatory Integrative and Comparative Physiology, Jun 1, 2005
Survival and some physiological responses to freezing were investigated in three European water f... more Survival and some physiological responses to freezing were investigated in three European water frogs (Rana lessonae, Rana ridibunda, and their hybridogen Rana esculenta). The three species exhibited different survival times during freezing (from 10 h for R. lessonae to 20 h for R. ridibunda). The time courses of percent water frozen were similar; however, because of the huge differences in body mass among species (from 10 g for Rana lessonae to nearly 100 g for Rana ridibunda), the ice mass accumulation rate varied markedly (from 0.75 Ϯ 0.12 to 1.43 Ϯ 0.11 g ice/h, respectively) and was lowest in the terrestrial hibernator Rana lessonae. The hybrid Rana esculenta exhibited an intermediate response between the two parental species; furthermore, within-species correlation existed between body mass and ice mass accumulation rates, suggesting the occurrence of subpopulations in this species (0.84 Ϯ 0.08 g ice/h for small R. esculenta and 1.78 Ϯ 0.09 g ice/h for large ones). Biochemical analyses showed accumulation of blood glucose and lactate, liver glucose (originating from glycogen), and liver alanine in Rana lessonae and Rana esculenta but not in Rana ridibunda in response to freezing. The variation of freeze tolerance between these three closely related species could bring understanding to the physiological processes involved in the evolution of freeze tolerance in vertebrates. Address for reprint requests and other correspondence: Y. Voituron, Physiologie des régulations énergétiques, cellulaires et moléculaires (U.M.R.
Biochimica Et Biophysica Acta - General Subjects, Aug 1, 2004
In organ transplantation, preservation injury is an important factor which could influence short-... more In organ transplantation, preservation injury is an important factor which could influence short-term and long-term graft outcome. The renal medulla is particularly sensitive to oxidant stress and ischemia-reperfusion injury (IRI). Using an autotransplant pig kidney model, we investigated renal function and medullary damage determined between day 1 and week 2 after 24-or 48-h cold storage in different preservation solutions: University of Wisconsin solution (UW), Hopital Edouard Herriot solution (a high Na + version of UW), ECPEG (high Na + preservation solution with PEG) and ICPEG (a high K + version of ECPEG) with or without trimetazidine (TMZ). TMZ improved renal preservation and increased renal function when added in each preservation solution (particularly HEH and ECPEG). Medullary damage led to the early appearance of trimethylamine-N-oxide (TMAO) followed by 1 H-NMR in urine and plasma. TMZ and ECPEG is the most efficient association to reduce medullary damage. This study clarifies the role of colloid and polarity solution and the role of mitochondrial protection by TMZ.
The aim of the study was to test a new preservation solution containing polyethylene glycol (S.C.... more The aim of the study was to test a new preservation solution containing polyethylene glycol (S.C.O.T. solution) as pancreatic islet isolation medium both to increase the islet yield and to prolong the allograft survival. In a model of islet transplantation in diabetic mouse, islets were isolated with S.C.O.T. in experimental groups and with Hank&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s balanced salt solution (HBSS) in control groups. The use of S.C.O.T. solution improved the islet yield (596+/-27 IEQ/pancreas) as compared to HBSS (456+/-11 IEQ/pancreas) (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.001). Allograft survival was prolonged in experimental group (17.3+/-4.3 days) versus controls (7.3+/-3.6 days) in a full mismatch combination (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.001) and in absence of recipient immunosuppression. The same prolongation (10 days) was also found in a strongly alloreactive transgenic combination. It is hypothesized that a transitory phenomenon of immunocamouflage of the graft surface antigens occurs, as shown by immunofluorescence studies. The use of this new solution could improve the results of islet transplantation in humans.
Journal of the American College of Cardiology, Jul 1, 1988
Although the introduction of cyclosporine has markedly improved survival in heart transplant reci... more Although the introduction of cyclosporine has markedly improved survival in heart transplant recipients, early detection of cardiac allogmtl rejection remains the major problem to be solved. Hitherto, the diagnosis of cardiac rejection has teen based on the results of cndomyocardial biopsy. which is considered as the only reliable test. However, ideally the detection of cardiac rejection should be noninvasive to allow frequent follow-up of the patients. Among the noninvasive techniques, neither the clectmcardiogram (I) nor the M-m& echccardiogram (2) is a reliable method for detection of acute cardiac rejection, especially in patients treated with cyclosporinc. Reports have shown yod correlation between h4hu io ha&e val8es altw bMluasu~ve therapy 4 < 0.05 and B < 0.0005. i-at~~tivel~). With 20% de. indexes of left ventricular filling obtained by Doppler echo. cardiography and those obtained by contrast angiography (3) or mdionuclide angiogmphy (4). Recently, analysis ofthc left ventriculardiastoli~ nmction with Doppler echacardiogsaphy has been shown (5) to be a useful and readily repeatable technique for the surveillance of cardiac recipients. Accordingly, we assessed the value of Doppler echocardiogmphy for detecting allograft cardiac rejection in 55 cardiac transplant recipients. Methods Study patieals. Fifty-five patients, who were examined by pulsed Doppler echocardiography and endomyocardial biopsy within 2 months after orthotopic cardiac ttansplantation were included in this study. Three subsas of p&ems were excluded from the srudy: IO patients (14%) with severe rejection associated with a major impairment of kn ventricular systolic function evaluated by echccardiographic imaging: 2 patients (3%) with brought to you by CORE View metadata, citation and similar papers at core.ac.uk
Analytical and Bioanalytical Chemistry, Nov 27, 2009
Metabolomics is a comprehensive method for metabolite assessment that involves measuring the over... more Metabolomics is a comprehensive method for metabolite assessment that involves measuring the overall metabolic signature of biological samples. We used this approach to investigate biochemical changes due to acute and chronic physical exercise. Twenty-two women using identical oral contraceptives were segregated into an untrained (n=10) or trained (n=12) group depending on their physical training background. The subjects performed two exercises in a randomized order: a prolonged exercise test (75% of their V Á O 2 max until exhaustion) and a short-term, intensive exercise test (short-term, intensive exercise anaerobic test). Urine specimens were collected before and 30 min after each test. The samples were analyzed by 1 H NMR spectroscopy, and multivariate statistical techniques were utilized to process the data. Distinguishing characteristics were observed only in the urine profiles of specimens collected before vs. 30 min after the short-term, intensive exercise test. The metabolites responsible for such changes were creatinine, lactate, pyruvate, alanine, β-hydroxybutyrate, acetate, and hypoxanthine. In both groups, the excretion of lactate, pyruvate, alanine, β-hydroxybutyrate, and hypoxanthine increased similarly after the completion of the short-term, intensive exercise test (p<0.03). However, acetate excretion increased to a lesser extent in trained than in untrained subjects (p< 0.05). In conclusion, metabolomics is a promising tool in order to gain insight into physiological status and to clarify the changes induced by short-term, intense physical exercise.
American Journal of Obstetrics and Gynecology, Feb 1, 1994
OBJECTIVES: To precisely evaluate fetal renal function in cases of bilateral fetal urinary tract ... more OBJECTIVES: To precisely evaluate fetal renal function in cases of bilateral fetal urinary tract obstruction, we analyzed fetal urine metabolites by nuclear magnetic resonance spectroscopy. STUDY DESIGN: Fifty-six specimens of fetal urine samples In utero were retrospectively analyzed by proton nuclear magnetic resonance. Group 1 (n = 21) consisted of survivors for a1 year with normal serum creatinine (<50 µmol/L), for group 2 (n = 17) of survivors with abnormal serum creatinine (>50 pmol/L), and group 3 (n = 18) of those with bilateral histologic dysplasla associated with fetal (termination of pregnancy) or neonatal death. RESULTS: A specific nuclear magnetic resonance profile was obtained for each of the three groups of renal function. A two-dimensional representation of alanine-valine and valine-threonine concentrations allows clear differentiation between the three degrees of renal function, with a sensitivity and specificity of 88% and 86%, respectively. CONCLUSIONS: In fetal bilateral urinary tract obstruction a fetal urine nuclear magnetic resonance profile allows an accurate identification of renal function at birth.
Citrate (Cit) is an organic acid normally found in urine and a central component of the tricarbox... more Citrate (Cit) is an organic acid normally found in urine and a central component of the tricarboxylic acid cycle. Changes in renal metabolism have been proposed to cause dramatic changes in Cit excretion with acid-base imbalances. Cold ischemia stimulates glycolysis and glycogenolysis and increases the production of lactic acid and the concentration of hydrogen ions with tissue acidification.
Abnormal movement of the mitral valve observed in patients with hypertrophic obstructive cardiomy... more Abnormal movement of the mitral valve observed in patients with hypertrophic obstructive cardiomyopathy (HOCM) consists of variable degrees of systolic anterior motion (SAM) towards the interventricular septum and has been recognized by angiography and echocardiography. Several explanations of its mechanism have been suggested: abnormal insertion of the anterior mitral leaflet (1), malposition of the papillary muscles (2), posterior displacement of the mitral annulus by septal hypertrophy (3) and venturi effect due to the high speed of ejection in the left ventricular outflow tract (4). A close correlation between SAM and the intraventricular pressure gradient has been demonstrated and SAM is also thought to play a part in the mechanism of mitral incompetence, the incidence of which increases with the severity of the disease (5).
Cellular and molecular biology (Noisy-le-Grand, France), 2004
In allogenic transplant the immediate immune response is due to the recipient T cell recognition ... more In allogenic transplant the immediate immune response is due to the recipient T cell recognition of non-self molecules presented on graft resident donor antigen presenting cells. An alternative to the transplantation tolerance paradigm is based on the development of strategies which distort alloimmune recognition of the graft by antigen reactive cells of the recipient. Immunocamouflage relies on the modification of the cell membrane surface with non-immunogenic molecules creating a barrier that prevents the recognition of antigenic sites by cells and antibodies of the recipient. Polymers can spontaneously bind to cell and tissues surfaces and sterically stabilize the underlying surface from interactions with other components in the surrounding. They can be adsorbed or chemically grafted to surfaces. Polyethylene glycol (PEG) seems to be the more effective at sterically stabilizing underlying surfaces. The outstanding protection provided by this polymer has been attributed to its mol...
... Sonia Borra, MD, David Hawkins, MD, FRCP (C), William Duguid, MB, B.CH., MRC (Path.), and Mic... more ... Sonia Borra, MD, David Hawkins, MD, FRCP (C), William Duguid, MB, B.CH., MRC (Path.), and Michael Kaye, MB, FRCP (C). ... On January 30, the total white-cell count was 5600, with 15 per cent eosinophils. ... 2. Schreiner GE, Maher JF: Toxic nephropathy . ...
Multidrug resistance (MDR) confers resistance to anticancer drugs and reduces therapeutic efficie... more Multidrug resistance (MDR) confers resistance to anticancer drugs and reduces therapeutic efficiency. It is often characterized by the expression of the MDR1 gene product P-glycoprotein (or gp170) at the membrane of tumor cells. To further propose a potential complementary tool in cancer treatment, the sensitivity of gp170 tumor cells to NK-dependent lysis was investigated. Two kinds of cells were generated from wild-type K562 erythroleukemic cells: the first were derived from Taxol-selected cells and cloned, whereas the second were retrovirally transduced by the cDNA of the MDR1 gene. The last process was also applied to the human embryonal carcinoma cells called Tera-2 cells. First, both cloned and MDR-1 K562 cells appeared highly susceptible to naive NK cell killing. Interestingly, in addition, Tera-2 cells that were not sensitive to NK lysis could be killed when they expressed gp170 at their membranes. In previous data, we demonstrated that NK cell release of bimolecular complex...
Transplant international : official journal of the European Society for Organ Transplantation, 2015
Introduction: Ischemia reperfusion (IR) injury is unavoidable during organ transplantation and le... more Introduction: Ischemia reperfusion (IR) injury is unavoidable during organ transplantation and leads to complications. With the increased use of marginal donors, more sensitive to IR, solutions must be found to improve outcome. We hypothesized that a higher preservation temperature could offer better protection against IR.[br/]Methods: We tested this in an in vitro model of IR using primary endothelial cells and in ex vivo preserved pig kidneys. In both, 24 h preservation in University of Wisconsin solution was used.[br/]Results: In vitro, compared to 4°C, temperatures between 19 and 32°C provided higher protection against cell death (LDH release test), permitting better mitochondrial function (complexes II and V activity tests) and a lowerexpression of endothelial activation and inflammation markers TLR4, MCP1 and ICAM1. Ex vivo, however, the superiority of 19 or 32°C was lost, as preserved pig kidneys showed similar levels of tissue damage (both tubulardilatation, loss of brush border and endoluminal detachment) during preservation, and at 24 h the 4°C kidneys displayed a trend towards less damage. In addition, the tissular Monocyte/Macrophage infiltration was increased in the 19°C and more in the 32°C temperature conservation as compared to 4°C storage.[br/]Conclusion: Our study shows that although a higher preservation temperature is preferable for cell survival and function, whole organ testing demonstrates that conceptual work needs to be performed to harness the potential of sub-normothermia
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