Papers by Emmanuel Effiong Edet
African Journal of …, 2009
... University of Nigeria, Nsukka. Okafor JC, Okolo HC, Ejiofor MAN, van der Maesen LJG, van der ... more ... University of Nigeria, Nsukka. Okafor JC, Okolo HC, Ejiofor MAN, van der Maesen LJG, van der Burgt XM, van Medenbach de Rooy JM (1994). ... Creatine kinase in serum. Clin. Chem. 22: 650 656. Ugochukwu NH, Babady NE, Cobourne MK, Gasset SR (2003). ...
Asian Journal of Biological Sciences, Dec 31, 2019
Asian Journal of Biological Sciences
... Effect of Gongronema latifolium Leaf Extract on some Liver Enzymes and Protein Levels in Diab... more ... Effect of Gongronema latifolium Leaf Extract on some Liver Enzymes and Protein Levels in Diabetic and non Diabetic Rats EEEdet1, IJ Atangwho1*, MI Akpanabiatu2,TE Edet3, FE Uboh1, E. David-Oku. ... PE Ebong, GE Egbung, MU Eteng and EU Eyong, 2007c. ...
The chemical composition and nutritive value of the seeds of African breadfruit, Treculia african... more The chemical composition and nutritive value of the seeds of African breadfruit, Treculia africana, Decne var. Africana, have been investigated.
Analysis of the seeds showed that they contained, in terms of dry weight, 13·4% protein, 18·9% lipid, 58·1% carbohydrate, 1·4% crude fibre and 2·1% ash. The moisture content was found to be 7·8% in terms of wet weight.
The mineral elements determined were Na, 7·0mg%; Mg, 184mg%; Ca, 17·5mg%; K, 585mg%; P, 382mg%; Cu, 3·9mg%; Fe, 1·6mg%; Cr, 0·20mg% and Zn, 7·5mg%.
Vitamins B1, 0·5mg%; B2, 0·3mg% C, 45·1mg% and β-carotene, 6·0mg% were found to be present in the seeds. The levels of some toxic substances in the seeds were oxalate (soluble), 2·5mg% (total), 3·0mg%; phytate, 2·0mg%; tannin, 15·0mg% and HCN, 1·1mg%. The amino acid composition of the seeds has been studied and the results show that some amino acids are present at high concentrations whilst others are present at only low, or undetectable, levels.
Effect of ethanolic leaf extract of Anacardium occidentale on histoarchitecture of brain and kidn... more Effect of ethanolic leaf extract of Anacardium occidentale on histoarchitecture of brain and kidney. Journal of pharmaceutical and biomedical sciences (J Pharm Biomed Sci.) 2013, May (Supplement 1); 30(30): S31-S35.
This work assessed the effect of Gongronema latifolium Benth et Hook (Asclepiadaceae) leaf-extrac... more This work assessed the effect of Gongronema latifolium Benth et Hook (Asclepiadaceae) leaf-extract on the histology of pancreas, kidney, heart and liver tissues of diabetic and non-diabetic male rats. The pancreas of diabetic rats which showed reduction in beta-cell density and size, distorted reticular support and infiltration of inflamed cells, recovered, showing distinct and highly populated beta-and αlpha-cells upon treatment with 400mg/kg body wt extract. The renal tubules of the diabetic rat kidneys indicated inflammation and obscured borders between convoluted tubules. However, gavaging with the extract caused a restoration and regeneration of these hitherto inflamed cells. Hyperglycaemia as well as extract administration caused no significant change in the heart tissues within the period, except that the myocytes of the extract-treated rats were multinucleated and hypertrophied. No significant change was also observed in the histology of the liver. Result of phytochemical screening of leafextract revealed the presence of alkaloids, saponins, tannins, phlobatannins, cardiac glycosides, reducing sugars and polyphenols in both ethanol and aqueous extracts. However, polyphenols and saponins were relatively higher in ethanol extract compared to the aqueous. The possible regenerative, protective and restorative effect of extract against hyperglycaemia-induced tissue toxicity may be attributable to these phytochemicals.
et al. Clinical significance of the effect of Gongronema latifolium leaf extract on some serum en... more et al. Clinical significance of the effect of Gongronema latifolium leaf extract on some serum enzymes in diabetic rats.. Journal of pharmaceutical and biomedical sciences (J Pharm Biomed Sci.) 2013, April; 29(29): 781-787. (Article no 12)
The effect of Gongronema latifolium on serum cardiac enzymes in alloxan induced diabetic rat mode... more The effect of Gongronema latifolium on serum cardiac enzymes in alloxan induced diabetic rat models and normal control rats were determined using graded doses of 80% ethanol extract of G. latifolium leaf. The serum activities of aspartate aminotransferase (AST), creatine kinase (CK), creatine kinase isoenzyme (CKMB) and lactate dehydrogenase (LD) were determined in diabetic Wistar albino rats; diabetic control and normal Wistar albino rats after 2 weeks administration of G. latifolium leaf extract. Serum CKMB and LD activities of all the experimental groups significantly (p 0.001) increased in diabetic rats when compared to non-diabetic rats. CK and LD decreased significantly in diabetic and non-diabetic rats treated with G. latifolium leaf extract when compared with the control. Equally, CKMB and LD of diabetic rats treated with G. latifolium leaf extract decreased significantly except CMB of group 4 and LD of group 3 animals. There were significant (p 0.001) decreased activity of serum AST in diabetic rats when compared to the non-diabetic rats. However, AST did not change significantly in non-diabetic rats while in diabetic rats AST increased significantly (p 0.001) when compared with their respective controls. These data suggest that the effects of G. latifolium leaf extract at our concentrations are not dose dependent and hepatotoxic. The extract of this plant is likely to be of biological significance in cardiovascular complication of diabetic and non-diabetic users.
The effect of leaf extract of Gongronema latifolium (GL) on some tissue enzymes and protein level... more The effect of leaf extract of Gongronema latifolium (GL) on some tissue enzymes and protein level of alloxan-induced diabetic rats was studied. Graded doses of the extract (200, 300 and 400 mg/kg b.
w.) were administered to the diabetic and corresponding non-diabetic rats for 14 days. At the end of which total protein, aminotransferases (ALT and AST), alkaline phosphatase (ALP) and gamma glutamyl transpeptidase (GGT) were assayed in the liver whole homogenate (LWH). Total liver protein concentration which was significantly increased (p<0.05) in untreated diabetic rats when compared to nondiabetic control rats was non significantly (p>0.05) changed in all test groups after treatment, compared to the control. Whereas in the non diabetic counterparts, protein level decreased in all treatment groups relative to the non diabetic control. However, this decrease was only significant (p<0.05) in the group administered 200mg/kg b. w. The decrease was therefore independent of the dose of extract. There was observed general significant decrease (p<0.001) in AST, ALT, ALP and GGT activities of diabetic test groups when compared to the corresponding non-diabetic groups. Compared to the diabetic control there was significant decrease in AST, ALT, ALP and GGT activities (p<0.001 for AST; p<0.05 for ALT, ALP and GGT) of diabetic rats administered 300mg/kg b. w. However at a test dose of 400mg/kg body weight, ALP and GGT activities rather increased significantly (p<0.05 for ALP; p<0.001 for GGT) relative to diabetic control. Hence the water soluble fraction of ethanol extract of Gongronema latifolium leaf is not likely to cause liver pathology, and can provide alleviation and protection to the animal liver in chemical diabetes with a dose up to 300 mg/kg b. w.
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Papers by Emmanuel Effiong Edet
Analysis of the seeds showed that they contained, in terms of dry weight, 13·4% protein, 18·9% lipid, 58·1% carbohydrate, 1·4% crude fibre and 2·1% ash. The moisture content was found to be 7·8% in terms of wet weight.
The mineral elements determined were Na, 7·0mg%; Mg, 184mg%; Ca, 17·5mg%; K, 585mg%; P, 382mg%; Cu, 3·9mg%; Fe, 1·6mg%; Cr, 0·20mg% and Zn, 7·5mg%.
Vitamins B1, 0·5mg%; B2, 0·3mg% C, 45·1mg% and β-carotene, 6·0mg% were found to be present in the seeds. The levels of some toxic substances in the seeds were oxalate (soluble), 2·5mg% (total), 3·0mg%; phytate, 2·0mg%; tannin, 15·0mg% and HCN, 1·1mg%. The amino acid composition of the seeds has been studied and the results show that some amino acids are present at high concentrations whilst others are present at only low, or undetectable, levels.
w.) were administered to the diabetic and corresponding non-diabetic rats for 14 days. At the end of which total protein, aminotransferases (ALT and AST), alkaline phosphatase (ALP) and gamma glutamyl transpeptidase (GGT) were assayed in the liver whole homogenate (LWH). Total liver protein concentration which was significantly increased (p<0.05) in untreated diabetic rats when compared to nondiabetic control rats was non significantly (p>0.05) changed in all test groups after treatment, compared to the control. Whereas in the non diabetic counterparts, protein level decreased in all treatment groups relative to the non diabetic control. However, this decrease was only significant (p<0.05) in the group administered 200mg/kg b. w. The decrease was therefore independent of the dose of extract. There was observed general significant decrease (p<0.001) in AST, ALT, ALP and GGT activities of diabetic test groups when compared to the corresponding non-diabetic groups. Compared to the diabetic control there was significant decrease in AST, ALT, ALP and GGT activities (p<0.001 for AST; p<0.05 for ALT, ALP and GGT) of diabetic rats administered 300mg/kg b. w. However at a test dose of 400mg/kg body weight, ALP and GGT activities rather increased significantly (p<0.05 for ALP; p<0.001 for GGT) relative to diabetic control. Hence the water soluble fraction of ethanol extract of Gongronema latifolium leaf is not likely to cause liver pathology, and can provide alleviation and protection to the animal liver in chemical diabetes with a dose up to 300 mg/kg b. w.
Analysis of the seeds showed that they contained, in terms of dry weight, 13·4% protein, 18·9% lipid, 58·1% carbohydrate, 1·4% crude fibre and 2·1% ash. The moisture content was found to be 7·8% in terms of wet weight.
The mineral elements determined were Na, 7·0mg%; Mg, 184mg%; Ca, 17·5mg%; K, 585mg%; P, 382mg%; Cu, 3·9mg%; Fe, 1·6mg%; Cr, 0·20mg% and Zn, 7·5mg%.
Vitamins B1, 0·5mg%; B2, 0·3mg% C, 45·1mg% and β-carotene, 6·0mg% were found to be present in the seeds. The levels of some toxic substances in the seeds were oxalate (soluble), 2·5mg% (total), 3·0mg%; phytate, 2·0mg%; tannin, 15·0mg% and HCN, 1·1mg%. The amino acid composition of the seeds has been studied and the results show that some amino acids are present at high concentrations whilst others are present at only low, or undetectable, levels.
w.) were administered to the diabetic and corresponding non-diabetic rats for 14 days. At the end of which total protein, aminotransferases (ALT and AST), alkaline phosphatase (ALP) and gamma glutamyl transpeptidase (GGT) were assayed in the liver whole homogenate (LWH). Total liver protein concentration which was significantly increased (p<0.05) in untreated diabetic rats when compared to nondiabetic control rats was non significantly (p>0.05) changed in all test groups after treatment, compared to the control. Whereas in the non diabetic counterparts, protein level decreased in all treatment groups relative to the non diabetic control. However, this decrease was only significant (p<0.05) in the group administered 200mg/kg b. w. The decrease was therefore independent of the dose of extract. There was observed general significant decrease (p<0.001) in AST, ALT, ALP and GGT activities of diabetic test groups when compared to the corresponding non-diabetic groups. Compared to the diabetic control there was significant decrease in AST, ALT, ALP and GGT activities (p<0.001 for AST; p<0.05 for ALT, ALP and GGT) of diabetic rats administered 300mg/kg b. w. However at a test dose of 400mg/kg body weight, ALP and GGT activities rather increased significantly (p<0.05 for ALP; p<0.001 for GGT) relative to diabetic control. Hence the water soluble fraction of ethanol extract of Gongronema latifolium leaf is not likely to cause liver pathology, and can provide alleviation and protection to the animal liver in chemical diabetes with a dose up to 300 mg/kg b. w.