Current Research Journal of Biological Sciences, 2013
This study reviews the role of Plant Growth Promoting Rhizobacteria (PGPR), Plant Growth Promotin... more This study reviews the role of Plant Growth Promoting Rhizobacteria (PGPR), Plant Growth Promoting Fungi (PGPF) and Mycorrhizal Fungi in the enhancement of rice growth and yield. A total 70 papers spanning from 1970 to 2013 was screened and noted its conclusion to enhance rice growth and yield. The mechanisms employed by microbes in enhancing rice growth and yield includes; the production of growth regulating substances, phosphate solubilization, N-fixation, cellulose degradation and side rophore production. Some microbes are also involved in cell regulation and signaling in rice plants. We can conclude that there are significant influences on the growth and yield of rice plant by microbes.
The anaerobic production of biohydrogen from different pretreated agroindustrial wastes, includin... more The anaerobic production of biohydrogen from different pretreated agroindustrial wastes, including rice bran (RB), de-oiled RB (DRB), sago starch (SS), and palm oil mill effluent (POME) via Clostridium saccharoperbutylacetonicum N1-4 was investigated in a batch culture system at 30°C and a pH of 6.2. A yield of 7627, 6995, and 6,363 mL H 2 /L was obtained from H 2 SO 4 (1 %)-treated DRB (10 %), enzymatically hydrolyzed DRB (10 %) and HCl (1 %)-treated DRB (10 %), respectively; however, untreated DRB (10 %) was able to produce only 3,286 mL H 2 /L. A strategic treatment of RB (10 %) with HCl (1 %) followed by enzymatic hydrolysis could produce 3,172 mL H 2 /L. An enzymatically hydrolyzed mixture of each POME and SS (5 %) produced 3,474 mL H 2 /L, and a remarkable enhancement of H 2 production (7,020 mL H 2 /L) was achieved when the same mixture was subjected to XAD-4 resin treatment. In contrast, the enzymatically hydrolyzed SS (5 %) could produce only 4,628 mL H 2 /L. Conclusively, it can be stated that agricultural wastes have a potential as substrates for biohydrogen production and that pretreatment with C. saccharoperbutylacetonicum N1-4 can contribute positively to enhancing the production.
Asian Journal of Microbiology Biotechnology and Environmental Sciences, Sep 24, 2014
This present was carried out to isolate and investigate antibiotic resistance pattern among Esche... more This present was carried out to isolate and investigate antibiotic resistance pattern among Escherichia coli O157:H7 strains isolated from imported frozen meat marketed in super markets in Klang valley, Malaysia. A total of 74 samples were purchased from various supermarkets and examined for presence E. coli 0157:H7 using Fluorocult medium which specific for E. coli O157:H7 and confirmed using API 20E kit. Confirmed isolates were evaluated for antibiotic resistance against 9 antibiotics. Results revealed that 35 out of 126 of the total isolates were confirmed E.coli O157:H7 with 22 (63%) from beef(10 from country A and 12 from country B), 13 isolates were from buffalo (8 from country A and 5 from country B). Results of antibiotic resistance showed that all isolates were found to be resistant to at least 2 antibiotics and were all resistant to Penicillin G. Resistance was more prevailing against Bacitracin (97.14%), Novobiocin (85.71%), Streptomycin (28.57%), Ampicillin (25.71%), Cephalothin (22.86%), Carbenicillin (17.14%), Sulphamethoxazole (14.29%) and Kanamycin (8.57%). Clustering analysis of the isolated bacteria from the samples using resistance to Cephalothin as base line revealed two clusters with cluster I having 4 sub clusters and cluster II having only 1 cluster. The high prevalence of multidrug resistance among E. coli O157:H7 pose serious danger to consumers' health. Further study on genetic bases of the antibiotic resistance is hence suggested.
Asian Journal of Microbiology, Biotechnology and Environmental Sciences
This present was carried out to isolate and investigate antibiotic resistance pattern among Esche... more This present was carried out to isolate and investigate antibiotic resistance pattern among Escherichia coli O157:H7 strains isolated from imported frozen meat marketed in super markets in Klang valley, Malaysia. A total of 74 samples were purchased from various supermarkets and examined for presence E. coli 0157:H7 using Fluorocult medium which specific for E. coli O157:H7 and confirmed using API 20E kit. Confirmed isolates were evaluated for antibiotic resistance against 9 antibiotics. Results revealed that 35 out of 126 of the total isolates were confirmed E.coli O157:H7 with 22 (63%) from beef(10 from country A and 12 from country B), 13 isolates were from buffalo (8 from country A and 5 from country B). Results of antibiotic resistance showed that all isolates were found to be resistant to at least 2 antibiotics and were all resistant to Penicillin G. Resistance was more prevailing against Bacitracin (97.14%), Novobiocin (85.71%), Streptomycin (28.57%), Ampicillin (25.71%), Cep...
Increasing demand of energy and awareness about environmental pollution has led to increase inter... more Increasing demand of energy and awareness about environmental pollution has led to increase interest in alternative, clean and renewable energy sources. Biobutanol is considered as the candidate liquid biofuel to replace gasoline. In this study, the capability of a newly isolated strain of local Clostridium acetobutylicum YM1 was tested to produce biobutanol in batch fermentation. Various culture conditions including glucose concentration, initial pH, incubation temperature and inoculum size were investigated for their effects on production of biobutanol using strain YM1. The results showed that the optimal biobutanol production was obtained at glucose concentration 50 g/L, initial pH 6.2, temperature 30°C and inoculum size 10%. These results show that C. acetobutylicum YM1 as a mesophilic bacterium is a potential candidate for biobutanol production.
Abstract. The cellulase production using sorghum straw as substrate by fungal culture of Aspergil... more Abstract. The cellulase production using sorghum straw as substrate by fungal culture of Aspergillus terreus SUK-1 was investigated in solid substrate fermentation (SSF). The optimum CMCase was achieved by testing most effective fermentation parameters which were: incubation temperature, pH and moisture content using Response Surface Methodology (RSM) based on Central Composite Design (CCD).The carboxymethyl cellulase activity (CMCase) was measured as the defining factor. The results were analysed by analysis of variance (ANOVA) and the regression quadratic model was obtained. The model was found to be significant (p<0.05) and the effect of temperature (25-40°C) and pH (4-7) was found to be not significant on CMCase activity whereas the moisture content was significant in the SSF conditions employed. The high yield of predicted CMCase activity (0.2 U/ml) was obtained under the optimized conditions (temperature 40 C, pH 5.4 and moisture content of 80%). The model was validated by ...
Current Research Journal of Biological Sciences, 2013
This study reviews the role of Plant Growth Promoting Rhizobacteria (PGPR), Plant Growth Promotin... more This study reviews the role of Plant Growth Promoting Rhizobacteria (PGPR), Plant Growth Promoting Fungi (PGPF) and Mycorrhizal Fungi in the enhancement of rice growth and yield. A total 70 papers spanning from 1970 to 2013 was screened and noted its conclusion to enhance rice growth and yield. The mechanisms employed by microbes in enhancing rice growth and yield includes; the production of growth regulating substances, phosphate solubilization, N-fixation, cellulose degradation and side rophore production. Some microbes are also involved in cell regulation and signaling in rice plants. We can conclude that there are significant influences on the growth and yield of rice plant by microbes.
The anaerobic production of biohydrogen from different pretreated agroindustrial wastes, includin... more The anaerobic production of biohydrogen from different pretreated agroindustrial wastes, including rice bran (RB), de-oiled RB (DRB), sago starch (SS), and palm oil mill effluent (POME) via Clostridium saccharoperbutylacetonicum N1-4 was investigated in a batch culture system at 30°C and a pH of 6.2. A yield of 7627, 6995, and 6,363 mL H 2 /L was obtained from H 2 SO 4 (1 %)-treated DRB (10 %), enzymatically hydrolyzed DRB (10 %) and HCl (1 %)-treated DRB (10 %), respectively; however, untreated DRB (10 %) was able to produce only 3,286 mL H 2 /L. A strategic treatment of RB (10 %) with HCl (1 %) followed by enzymatic hydrolysis could produce 3,172 mL H 2 /L. An enzymatically hydrolyzed mixture of each POME and SS (5 %) produced 3,474 mL H 2 /L, and a remarkable enhancement of H 2 production (7,020 mL H 2 /L) was achieved when the same mixture was subjected to XAD-4 resin treatment. In contrast, the enzymatically hydrolyzed SS (5 %) could produce only 4,628 mL H 2 /L. Conclusively, it can be stated that agricultural wastes have a potential as substrates for biohydrogen production and that pretreatment with C. saccharoperbutylacetonicum N1-4 can contribute positively to enhancing the production.
Asian Journal of Microbiology Biotechnology and Environmental Sciences, Sep 24, 2014
This present was carried out to isolate and investigate antibiotic resistance pattern among Esche... more This present was carried out to isolate and investigate antibiotic resistance pattern among Escherichia coli O157:H7 strains isolated from imported frozen meat marketed in super markets in Klang valley, Malaysia. A total of 74 samples were purchased from various supermarkets and examined for presence E. coli 0157:H7 using Fluorocult medium which specific for E. coli O157:H7 and confirmed using API 20E kit. Confirmed isolates were evaluated for antibiotic resistance against 9 antibiotics. Results revealed that 35 out of 126 of the total isolates were confirmed E.coli O157:H7 with 22 (63%) from beef(10 from country A and 12 from country B), 13 isolates were from buffalo (8 from country A and 5 from country B). Results of antibiotic resistance showed that all isolates were found to be resistant to at least 2 antibiotics and were all resistant to Penicillin G. Resistance was more prevailing against Bacitracin (97.14%), Novobiocin (85.71%), Streptomycin (28.57%), Ampicillin (25.71%), Cephalothin (22.86%), Carbenicillin (17.14%), Sulphamethoxazole (14.29%) and Kanamycin (8.57%). Clustering analysis of the isolated bacteria from the samples using resistance to Cephalothin as base line revealed two clusters with cluster I having 4 sub clusters and cluster II having only 1 cluster. The high prevalence of multidrug resistance among E. coli O157:H7 pose serious danger to consumers' health. Further study on genetic bases of the antibiotic resistance is hence suggested.
Asian Journal of Microbiology, Biotechnology and Environmental Sciences
This present was carried out to isolate and investigate antibiotic resistance pattern among Esche... more This present was carried out to isolate and investigate antibiotic resistance pattern among Escherichia coli O157:H7 strains isolated from imported frozen meat marketed in super markets in Klang valley, Malaysia. A total of 74 samples were purchased from various supermarkets and examined for presence E. coli 0157:H7 using Fluorocult medium which specific for E. coli O157:H7 and confirmed using API 20E kit. Confirmed isolates were evaluated for antibiotic resistance against 9 antibiotics. Results revealed that 35 out of 126 of the total isolates were confirmed E.coli O157:H7 with 22 (63%) from beef(10 from country A and 12 from country B), 13 isolates were from buffalo (8 from country A and 5 from country B). Results of antibiotic resistance showed that all isolates were found to be resistant to at least 2 antibiotics and were all resistant to Penicillin G. Resistance was more prevailing against Bacitracin (97.14%), Novobiocin (85.71%), Streptomycin (28.57%), Ampicillin (25.71%), Cep...
Increasing demand of energy and awareness about environmental pollution has led to increase inter... more Increasing demand of energy and awareness about environmental pollution has led to increase interest in alternative, clean and renewable energy sources. Biobutanol is considered as the candidate liquid biofuel to replace gasoline. In this study, the capability of a newly isolated strain of local Clostridium acetobutylicum YM1 was tested to produce biobutanol in batch fermentation. Various culture conditions including glucose concentration, initial pH, incubation temperature and inoculum size were investigated for their effects on production of biobutanol using strain YM1. The results showed that the optimal biobutanol production was obtained at glucose concentration 50 g/L, initial pH 6.2, temperature 30°C and inoculum size 10%. These results show that C. acetobutylicum YM1 as a mesophilic bacterium is a potential candidate for biobutanol production.
Abstract. The cellulase production using sorghum straw as substrate by fungal culture of Aspergil... more Abstract. The cellulase production using sorghum straw as substrate by fungal culture of Aspergillus terreus SUK-1 was investigated in solid substrate fermentation (SSF). The optimum CMCase was achieved by testing most effective fermentation parameters which were: incubation temperature, pH and moisture content using Response Surface Methodology (RSM) based on Central Composite Design (CCD).The carboxymethyl cellulase activity (CMCase) was measured as the defining factor. The results were analysed by analysis of variance (ANOVA) and the regression quadratic model was obtained. The model was found to be significant (p<0.05) and the effect of temperature (25-40°C) and pH (4-7) was found to be not significant on CMCase activity whereas the moisture content was significant in the SSF conditions employed. The high yield of predicted CMCase activity (0.2 U/ml) was obtained under the optimized conditions (temperature 40 C, pH 5.4 and moisture content of 80%). The model was validated by ...
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