Papers by Bogdan Parakhonskiy
Colloids and Surfaces A: Physicochemical and Engineering Aspects, Oct 1, 2022
To fabricate a new surface-enhanced Raman scattering (SERS)-active optical fiber sensor, the desi... more To fabricate a new surface-enhanced Raman scattering (SERS)-active optical fiber sensor, the design and preparation of SERS-active sensing layer is one of important topics. In this study, we fabricated a highly sensitive three-dimensional (3D) SERS-active sensing layer on the optical fiber terminal via in situ polymerizing a porous polymer material on a flat optical fiber terminal through thermal-induced process, following with the photochemical silver nanoparticles growth. The polymerized polymer formed a 3D porous structure with the pore size of 0.29-0.81 lm, which were afterward decorated with abundant silver nanoparticles with the size of about 100 nm, allowing for higher SERS enhancement. This SERS-active optical fiber sensor was applied for the determination of 4-mercaptopyridine, crystal violet and maleic acid The enhancement factor of this SERS sensing layer can be reached as about 10 8. The optical fiber sensor with high sensitive SERS-active porous polymer is expected for online analysis and environment detection.
Journal of Thermal Analysis and Calorimetry, Sep 19, 2016
Nanoscale heating governs a multitude of processes relevant to molecular states and biomolecular ... more Nanoscale heating governs a multitude of processes relevant to molecular states and biomolecular functionality. In this mini-review, we describe application of localized laser-nanoparticle heating for polymeric polyelectrolyte capsules and layers, covering the principles governing the nanoscale laser-induced heat generation, synthesis of nanoparticles and their adsorption on polyelectrolyte multilayer membrane and different applications of these effects. Release of encapsulated materials is considered as one of the methods of release, while its further application is linked, among others, with intracellular delivery and release of encapsulated peptides, remotely controlled reactors, polymeric surface properties and corrosion protection.
Colloids and Surfaces A: Physicochemical and Engineering Aspects, Oct 1, 2020
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Biotechnology Journal, Jul 10, 2018
Porous calcium carbonate (CaCO 3) vaterite particles are very attractive templates for the encaps... more Porous calcium carbonate (CaCO 3) vaterite particles are very attractive templates for the encapsulation of pharmaceuticals and for the construction of hollow polyelectrolyte capsules, sensors, and enzyme-catalyzed reactors. Although CaCO 3 is biocompatible and biodegradable, little is known about the intercellular behavior and properties of vaterite particles in the cytoplasm of cells. In this work, the authors combine confocal Raman and fluorescent microscopy for the imaging of porous CaCO 3 vaterite particles in HeLa cells to study the uptake and status of the particles inside the cells in real time. Analysis of the fluorescence images shows that the particles penetrated the plasma membrane 3 h after being added to the cell culture and that the internalization of the particles continued up to 48 h. The crystal structure of individual vaterite particles in the cytoplasm of HeLa cells did not obviously change for 144 h. For clusters of particles, however, the authors identify Raman spectroscopic signatures of the stable calcite phase after 72 h of incubation, confirming an ion-exchange mechanism of vaterite transformation to calcite. The results indicate that these imaging approach to examining inorganic particles in living cells may have theranostic applications.
EarthArXiv (California Digital Library), Apr 25, 2022
Research on microplastics (MP) in soils is much complicated due to the lack of dedicated (extract... more Research on microplastics (MP) in soils is much complicated due to the lack of dedicated (extraction) methodologies and the strong matrix interferences for MP detection, and there is almost no research on the dynamics of the smallest MP in soil. Here we compared the possible detection of the smallest MP fraction (1-2 µm) by µ-Raman spectroscopy and fluorescence microscopy in matrices of highly varying complexity. Samples of pure quartz sand, soil with removal of native soil organic matter (SOM), and soil with native SOM still present were amended with fluorescent polystyrene (PS) microparticles (diameter 1.65±0.04 µm) in different concentrations ranging from 0.1 to 0.001%, and after mixing and compaction both the Raman spectra and fluorescence microscopy images were obtained. Characteristic PS Raman peaks (main peak at 1009 cm-1) were visible in quartz sand (all concentrations) and soil without SOM (highest concentration only), but not in the other situations, whereas fluorescence microscopy clearly visualized the MP at all concentrations in all matrices. The possibility of direct and unambiguous fluorescent MP detection in real soil also circumvents the need for lengthy extraction procedures, and opens up new avenues for studying mechanistic aspects of the smallest MP fractions in soil.
Advanced Materials Interfaces, Aug 1, 2021
Nanofibrillar structures are of importance in biomedicine, including lung, cardiovascular, liver,... more Nanofibrillar structures are of importance in biomedicine, including lung, cardiovascular, liver, skin, neuroscience research, and tissue engineering. Developing advanced materials and interfaces should contribute to uncovering the mechanisms of diseases aiming to find cure. The similarity between the extracellular matrix (ECM) of soft tissue and hydrogels, characterized by a high water‐content viscoelastic polymeric fiber, has stimulated the development of hydrogels for biomedical applications. However, most hydrogels have a meshy structure resulting in poor cell adhesion properties. Here, fabrication of gellan gum (GG) hydrogels arranged by thermally driven self‐assembly into a network of nanofibers is reported. Mechanical properties of such nanofibrillar hydrogels are analyzed on micro‐ and macroscales. As a result, and in sharp contrast to commonly produced meshy GG hydrogels, the nanofiber‐based hydrogels facilitate the adherence and lead to proliferation of cells. This is assigned to microstructural rearrangements characterized by a changing density and pore size decrease, accompanied with a lower water content. Cell growth on such nanofibrillar structures is investigated for osteoblasts, which are chosen as a model system. The developed nanofibrous interfaces in this study are envisioned to be applicable for growing various types of cells and they should contribute to better understanding cell interactions with ECM.
Polymers, Mar 9, 2020
In this review, materials based on polymers and hybrids possessing both organic and inorganic con... more In this review, materials based on polymers and hybrids possessing both organic and inorganic contents for repairing or facilitating cell growth in tissue engineering are discussed. Pure polymer based biomaterials are predominantly used to target soft tissues. Stipulated by possibilities of tuning the composition and concentration of their inorganic content, hybrid materials allow to mimic properties of various types of harder tissues. That leads to the concept of "one-matches-all" referring to materials possessing the same polymeric base, but different inorganic content to enable tissue growth and repair, proliferation of cells, and the formation of the ECM (extra cellular matrix). Furthermore, adding drug delivery carriers to coatings and scaffolds designed with such materials brings additional functionality by encapsulating active molecules, antibacterial agents, and growth factors. We discuss here materials and methods of their assembly from a general perspective together with their applications in various tissue engineering sub-areas: interstitial, connective, vascular, nervous, visceral and musculoskeletal tissues. The overall aims of this review are twofold: (a) to describe the needs and opportunities in the field of bio-medicine, which should be useful for material scientists, and (b) to present capabilities and resources available in the area of materials, which should be of interest for biologists and medical doctors.
Advanced Healthcare Materials, Jun 13, 2023
Macromolecular Materials and Engineering, Sep 18, 2020
The possibilities created by tuning the architecture of hydrogels open up extensive opportunities... more The possibilities created by tuning the architecture of hydrogels open up extensive opportunities for their application in tissue engineering and biomedicine. A simple and effective procedure is developed here, based on thermal annealing, which affects the alignment of the fibrils and results in a mesh‐like structure. Atomic force microscopy and cryo‐scanning electron microscopy are used to characterize the resultant gel. The alignment angle is demonstrated to take place spontaneously, providing an easy way for self‐assembly of such structures. The protocol developed here is likely to be applicable in numerous areas of biomedicine and tissue engineering.
Advanced Materials Interfaces, Jun 14, 2021
Nanofibrillar structures are of importance in biomedicine, including lung, cardiovascular, liver,... more Nanofibrillar structures are of importance in biomedicine, including lung, cardiovascular, liver, skin, neuroscience research, and tissue engineering. Developing advanced materials and interfaces should contribute to uncovering the mechanisms of diseases aiming to find cure. The similarity between the extracellular matrix (ECM) of soft tissue and hydrogels, characterized by a high water‐content viscoelastic polymeric fiber, has stimulated the development of hydrogels for biomedical applications. However, most hydrogels have a meshy structure resulting in poor cell adhesion properties. Here, fabrication of gellan gum (GG) hydrogels arranged by thermally driven self‐assembly into a network of nanofibers is reported. Mechanical properties of such nanofibrillar hydrogels are analyzed on micro‐ and macroscales. As a result, and in sharp contrast to commonly produced meshy GG hydrogels, the nanofiber‐based hydrogels facilitate the adherence and lead to proliferation of cells. This is assigned to microstructural rearrangements characterized by a changing density and pore size decrease, accompanied with a lower water content. Cell growth on such nanofibrillar structures is investigated for osteoblasts, which are chosen as a model system. The developed nanofibrous interfaces in this study are envisioned to be applicable for growing various types of cells and they should contribute to better understanding cell interactions with ECM.
Annalen der Physik, Oct 29, 2012
Nanoplasmonic biosensors based on gold nanoparticle functionalized smooth silica and porous calci... more Nanoplasmonic biosensors based on gold nanoparticle functionalized smooth silica and porous calcium carbonate particles are presented. It is identified in this comparative study the role of porosity for adsorbing gold nanoparticles and subsequent detection of biomarkers. That is further applied in this study for detection of biomarkers. Detection of glucose-a biomarker of diabetes is studied together with that of bovine serum albumin-a very relevant biomolecule. Raman scattering is used for label-free detection of molecules in the sub-μM-mM range detection capabilities, which covers the range corresponding to healthy and diseased persons. Implications of current study for detection and identification of biomarkers are discussed.
nano Online, 2016
Background: Recent reports highlighting the role of particle geometry have suggested that anisotr... more Background: Recent reports highlighting the role of particle geometry have suggested that anisotropy can affect the rate and the pathway of particle uptake by cells. Therefore, we investigate the internalization by cells of porous calcium carbonate particles with different shapes and anisotropies. Results: We report here on a new method of the synthesis of polyelectrolyte coated calcium carbonate particles whose geometry was controlled by varying the mixing speed and time, pH value of the reaction solution, and ratio of the interacting salts used for particle formation. Uptake of spherical, cuboidal, ellipsoidal (with two different sizes) polyelectrolyte coated calcium carbonate particles was studied in cervical carcinoma cells. Quantitative data were obtained from the analysis of confocal laser scanning microscopy images. Conclusions: Our results indicate that the number of internalized calcium carbonate particles depends on the aspect ratio of the particle, whereby elongated particles (higher aspect ratio) are internalized with a higher frequency than more spherical particles (lower aspect ratio). The total volume of internalized particles scales with the volume of the individual particles, in case equal amount of particles were added per cell.
Chemical Communications, 2023
Various methods of encapsulation into and release from polyelectrolyte multilayer capsules are us... more Various methods of encapsulation into and release from polyelectrolyte multilayer capsules are used to develop diverse applications.
The development of novel carriers for the targeted photosensitizer delivery is a significant prob... more The development of novel carriers for the targeted photosensitizer delivery is a significant problem in a modern photodynamic therapy. Such a nanomedicine research is aimed to reduce the sides effects connected with insufficient selectivity of action for the applied dye. Porous calcium carbonate (CaCO3) particles were proposed as matrices for the photosensitizer “Photosens” encapsulation. Cellular uptake and phototoxicity of the carriers were investigated using NIH/3T3 cells. The local concentration of the dye inside of the cell was increased by the usage of Photosens-loaded CaCO3 particles in comparison with the “free” Photosens solution, due to the fact, that the particles trapped by cells provided the dye localization inside. Cellular experiments demonstrated high penetration of containers into the cells within 24h. The point-wise laser (670 nm, 3 mW, 5 min) effect on cells was investigated by confocal laser scanning microscopy. Significant phototoxic effect was shown for Photosens-loaded CaCO3 particles allowing controlled consequential cell destroying by the laser. The demonstrated effect hold out the prospect for the application of proposed system in anticancer PDT.
Nanoscale, 2022
Nanoarchitectonics, like architectonics, allows the design and building of structures, but at the... more Nanoarchitectonics, like architectonics, allows the design and building of structures, but at the nanoscale.
Materials Today Communications
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Papers by Bogdan Parakhonskiy