We herein describe the case of a 60-year-old man who presented clinical and histopathological evi... more We herein describe the case of a 60-year-old man who presented clinical and histopathological evidence of Henoch-Schönlein purpura. Antineutrophil antibodies (ANCA) showed positive results on an enzyme-linked immunosorbent assay and immunofluorescence for anti-myeloperoxidase and anti-proteinase 3 antibodies. Dual positivity for both cytoplasmatic (C-ANCA) and perinuclear (P-ANCA) antineutrophil antibodies has been found previously in a small number of reports, but to our knowledge this is the first time the simultaneous presence of C-ANCA and P-ANCA has been observed in Henoch-Schönlein purpura.
The Journal of Allergy and Clinical Immunology, Feb 1, 2003
inflammatory disease with several etiopathogenic mechanisms. Considering its heterogeneity, it ha... more inflammatory disease with several etiopathogenic mechanisms. Considering its heterogeneity, it has been recently used other designation for this pathology-atopic eczema/dermatitis syndrome (AEDS). The association with food and respiratory allergy is variable among different populations. PURPOSE: To analyze a group of patients referred to our clinic with AEDS in order to evaluate the association of this syndrome with food allergy and respiratory diseases. METHODS: From the total number of first visits to our department during 2000-2001 (n=3436) we selected all patients with a history of AEDS. We analyzed age, gender, presence of food and respiratory allergy and skin prick tests (SPT). RESULTS: We found 193 patients with a mean age of 7,5 years old (1-54 years), female/male ratio 1/1,5. There were 68 patients with isolated AEDS (35,8%). Associated respiratory disease was identified in 113 patients (58,5%) and food allergy in 19 patients (9,8%)-most cases with urticaria/angioedema. SPT were positive to aeroallergens in 64% and to food allergens in 16% of the sample. We found positive SPT results in 47,8% of patients with isolated AEDS, in 84,2% of patients with food allergy and in 92% with respiratory disease. CONCLUSIONS: In contrast to other series, we found a low prevalence of food allergy, in most cases manifested by immediate reactions. More than half of our patients have respiratory allergic diseases associated to a higher prevalence of sensitization to aeroallergens. These results reflect the heterogeneity of AEDS populations.
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Galectin-3 is an animal lectin, formerly named ε-binding protein or Mac-2, which has been describ... more Galectin-3 is an animal lectin, formerly named ε-binding protein or Mac-2, which has been described to play an important role in some inflammatory processes by the implication of different cells and the increase in cell adhesion functions through laminin binding activity. In this work we analyzed the role of galectin-3 in the modulation of Th2 cytokines that have an important role in the development of the inflammatory response. We have found that the addition of galectin-3 to human eosinophils, the eosinophilic cell line EoL-3, PBMC, and an Ag-specific T cell line (CD4+) produced a selective inhibition of IL-5 transcription. No inhibitory effect was found on the IL-4 mRNA transcription rate. The inhibitory effect on IL-5 transcription was reversed by incubation with lactose and using specific Ab against galectin-3. Galectin-3 is able to induce inhibition of the IL-5 released in the supernatants from PBMC stimulated with phorbol 12,13-dibutyrate and anti-CD3. Similar results were ob...
Additional file 1: Supplementary Table 1. Comparison between Phases I and II for clinical and dem... more Additional file 1: Supplementary Table 1. Comparison between Phases I and II for clinical and demographic characteristics.
Background Several antibodies have proven useful in autoimmune diseases, as markers for diagnosis... more Background Several antibodies have proven useful in autoimmune diseases, as markers for diagnosis, prognosis or clinical manifestations. Objectives To evaluate the clinical utility and the various associations of antibodies SSA/Ro52, SSA/Ro60 and SSB/La in LES at a referral hospital in Spain. Methods We retrospectively analise the patterns of all the consecutive samples submitted to the Immunology Unit for antinuclear antibody screening between 2002 and 2012. ANA positive sera for indirect immunofluorescence test (IFI) were analised with a commercial immunoassay (Innolia-ANA update). 323 patients had more than one positive sample for some of the autoantibodies Ro52, Ro60 or La and the pattern of sera for each patient were virtually identical. 113 patients with diagnosis of LES were included in the study. Dependent variables: Each of the possible combinations of results of Ro52, Ro60 and La, ie: 52+/60+/La+; 52+/60+/La-; 52+/60-/La+; 52+/60-/La-; 52-/60+/La+; 52-/60+/La-; 52-/60-/La+. Independent variables: age, sex, diagnostic variables, pattern by IIF, result of the immunoassay, clinical features and laboratory variables. Results Of the 113 patients, 93,8% were women and 6,3% males, aged between 16 and 71 years (mean: 37,7; dev. Typical 13.9) in the moment of the diagnosis. The most frequent pattern were fine speckle, 68 cases (61,3%) and homogeneous 41 (36,6%) The relation of clinical symptoms and the positivity of Ro52, Ro60 and La in their various combinations (Table 1) showed the following statistical association: xerostomia and rheumatoid factor + with Ro52+/Ro60-/La+; arthritis with Ro52+/Ro60+/La-; xerostomia, xerophthalmia, hypergammaglobulinemia and rheumatoid factor with isolated Ro52+; pericardial effusion, subacute cutaneous lesions and hypocomplementemia with Ro52-/Ro60+/La-. In relation to IFI, fine speckeled pattern was positively associated with Ro52+/Ro60+/La+ combination (p 0,003), and La+ isolated (p 0,03). Conclusions Our study found significant relationship between clinical and laboratory manifestations and different patterns of antibodies to Ro52, Ro60 and SSB. The combination of antibodies might be clinically useful because these associations have prognostic and therapeutic implications. Would be worthwhile to explore its usefulness in other ethnic groups. Disclosure of Interest None Declared
Esophageal benign tumors appear very infrequently and, in contrast to malignant ones, the majorit... more Esophageal benign tumors appear very infrequently and, in contrast to malignant ones, the majority are asymptomatic. Leiomyomas, papillomas, and adenomas are the most frequent among them; angiomas, fibromas, schwannomas (neurogenic tumors), lipomas, or glandular retention cysts are much less frequent. Most esophageal polyps are fibrovascular ones (1), are located in the proximal third of the esophagus, and frequently present with symptoms of regurgitation (2). An inflammatory fibroid polyp is an entity that was first described by Vanek as a "gastric submucosal granuloma with eosinophilia" (3). It has variously been called eosinophilic granuloma, granuloblastoma, gastric neurofibroma, xanthofibroma, etc (4). This condition is very seldom found, and its most frequent gastrointestinal localization is the stomach, the small intestine and, less frequently, the colon. However, some cases have been reported of esophageal (5-7), duodenal (8), and rectal (9) localization. We present the case of a patient with an esophageal inflammatory fibroid polyp who showed neither dysphagia nor reflux symptoms and we note the endoscopic and radiologic aspects of the polyp. CASE REPORT A male patient, age 60 years, was sent to our center for surgical treatment of a possible esophageal neoplasm. He usually smoked 20 cigarettes a day and was a moderate alcohol consumer. His personal history showed that he had been submitted to surgery three years before because Manuscript
Background The amino-acid and nucleotide sequence of Ole e 1 (the major antigen of olive pollen) ... more Background The amino-acid and nucleotide sequence of Ole e 1 (the major antigen of olive pollen) has been described and the IgE antibody response to this major allergen was associated with DR7/DQ2 antigens. With this previous data we try to define the T-cell epitopes implicated in Ole e 1 reactivity. Objectives To study the recognition of T cells (derived from allergic and non-allergic Ole e 1 patients) to Ole e 1 synthetic peptides in order to define immunodominant T-cell epitopes. Methods We have compared the proliferative response of the peripheral blood mononuclear cells from Ole e 1 sensitized patients vs. non-sensitized controls, induced by 14 Ole e 1 synthetic peptides. Thirty subjects were classified in two groups: group 1 (nonresponders against Ole e 1, n ¼ 16) and group 2 (Ole e 1 responders, n ¼ 14), according to their clinical parameters and the presence or not in their sera of the significant Ole e 1 IgE antibody levels. Results Our results shown that it is possible to find T cells reactive to Ole e 1 peptides in patients with and without significant levels of Ole e 1 IgE antibodies. However, the percentage of response was higher in patients with IgE antibodies 71.4% vs 25%), and the recognition profile was different: the control group showed a broad reactivity pattern, in contrast, the response by the 'Ole e 1 responders' group was mainly directed against three peptides of the carboxi-terminal region, peptides 10 (91-102), 12 (109-120) and 13 (119-130), with a response frequency of 35.7, 28.5 and 28.5%, respectively. By direct and inhibition test no antibody response was found against the synthetic peptides. Conclusions Our data suggest that the regions between 91 and 102 and 109-130 aminoacids on the Ole e 1 molecule are immunodominant T-cell epitopes. These epitopes are not recognized by IgE antibodies.
Expression of B7 molecules plays an important role in antigen presentation and T cell proliferati... more Expression of B7 molecules plays an important role in antigen presentation and T cell proliferation. Although eosinophils (co.,,) have been reported to express HLA-DR on activation and to present antigens to T cells, little is known about their co-stimulalory properties. The purpose of this stmlv was to determine whelher eos express B7 molecules and io assess their role in antigen presentation. Four normal volunteers and one hyperlgE syndrome patient were evaluated. Eos prep'lrations of 97-99% purity were obtained by immunomagnelic separation with anti-CD16, cultured with It.3, stained directly with HLA-DR, B7-2 or B7-1 antibodies nnd analyzed by FACS. No expression of I-II.A-DF,, B7-2 or BT-I was noted on freshly isolated cos. Bv contrast, 72-96 hours later, IL-3 induced the expression of HLA-DR in 70 + 15% and B7-2 in 25 + 8 % of the cos, but not B7-1. Furthermore, Eos supported T cell prolifenuion to the super:mtigen TSST-I. The proliferation was inhibited 50% by CTLA4Ig and anti-B7-2, but not by an isotype control. We conclude that hurnan cos express 137-2 which provides co-stinmlator 3, signals to T cells for activation and proliferation. Studies are in progress to elucidate the mechanisms of T cell activation by eos via the B7-2 pathway.
Authors: Gallardo S.; del Pozo V.; Cardaba B.; Jurado A.; Cortegano I.; del Amo A.; Arrieta I.; P... more Authors: Gallardo S.; del Pozo V.; Cardaba B.; Jurado A.; Cortegano I.; del Amo A.; Arrieta I.; Palomino P.; Lahoz C. ... This article is hosted on another website. ... You may be required to register, activate a subscription or purchase the article before you can obtain the full text.
Galectin-3 is an animal lectin, formerly named ⑀-binding protein or Mac-2, which has been describ... more Galectin-3 is an animal lectin, formerly named ⑀-binding protein or Mac-2, which has been described to play an important role in some inflammatory processes by the implication of different cells and the increase in cell adhesion functions through laminin binding activity. In this work we analyzed the role of galectin-3 in the modulation of Th2 cytokines that have an important role in the development of the inflammatory response. We have found that the addition of galectin-3 to human eosinophils, the eosinophilic cell line EoL-3, PBMC, and an Ag-specific T cell line (CD4 ؉) produced a selective inhibition of IL-5 transcription. No inhibitory effect was found on the IL-4 mRNA transcription rate. The inhibitory effect on IL-5 transcription was reversed by incubation with lactose and using specific Ab against galectin-3. Galectin-3 is able to induce inhibition of the IL-5 released in the supernatants from PBMC stimulated with phorbol 12,13-dibutyrate and anti-CD3. Similar results were obtained when a T-specific cell line was stimulated with Ag. Also, EoL-3 stimulated with anti-CD32 produced IL-5 protein, the synthesis of which was partially inhibited by galectin-3. The present results demonstrate that galectin-3 induces a selective down-regulation of IL-5 expression in different cell types, opening important new possibilities in the regulation of the allergic reactions.
AUTHORS. Aurora Jurado1,2, María C. Martín1,3, Cristina Abad-Molina4, Antonio Orduña4, Alba Martí... more AUTHORS. Aurora Jurado1,2, María C. Martín1,3, Cristina Abad-Molina4, Antonio Orduña4, Alba Martínez5, Esther Ocaña5, Oscar Yarce2, Ana M. Navas2 , Antonio Trujillo2, Luis Fernández6, Esther Vergara6 , Beatriz Rodríguez7, Bibiana Quirant8, Eva Martínez8, Manuel Hernández9, Janire Perurena-Prieto9, Juana Gil10, Sergi Cantenys10, Gema GonzálezMartínez11, María T. Martínez-Saavedra11, Ricardo Rojo12, Francisco M. Marco13, Sergio Mora13 Jesús Ontañón14, Marcos López-Hoyos15, Gonzalo Ocejo-Vinyals15,Josefa Melero16, Marta Aguilar16, Delia Almeida17, Silvia Medina17, María C. Vegas18, Yesenia Jiménez18, Álvaro Prada19, David Monzón19, Francisco Boix20, Vanesa Cunill21, Juan Molina2 1These authors contributed equally to this manuscript. Affiliations.
OBJECTIVES ANA are the most extensively used test for the diagnosis of systemic autoimmune diseas... more OBJECTIVES ANA are the most extensively used test for the diagnosis of systemic autoimmune diseases. However, testing by indirect immunofluorescence assays (IIFAs) on HEp-2 cells, the gold standard test, is time-consuming and needs expertise. Thus there is a trend to replace it with other automated solid-phase assays directed against specific ANA. Nonetheless, the Hep-2 cell is an autoantigen array and ANA have been classified into 29 types, some of them with no clear association with a specificity to be detected. It is especially in these uncommon patterns where no clinical relationship is found and no antigenic specificity is detected. Here we retrospectively collected clinical data from patients with confirmed uncommon HEp-2 IIFA patterns to search for an associated clinical condition. METHODS We conducted an observational retrospective study including 608 patients with organ-specific and non-organ-specific autoimmune diseases (OSADs and NOSADs, respectively) with a confirmed rar...
Background One hundred fifty million contagions, more than 3 million deaths and little more than ... more Background One hundred fifty million contagions, more than 3 million deaths and little more than 1 year of COVID-19 have changed our lives and our health management systems forever. Ageing is known to be one of the significant determinants for COVID-19 severity. Two main reasons underlie this: immunosenescence and age correlation with main COVID-19 comorbidities such as hypertension or dyslipidaemia. This study has two aims. The first is to obtain cut-off points for laboratory parameters that can help us in clinical decision-making. The second one is to analyse the effect of pandemic lockdown on epidemiological, clinical, and laboratory parameters concerning the severity of the COVID-19. For these purposes, 257 of SARSCoV2 inpatients during pandemic confinement were included in this study. Moreover, 584 case records from a previously analysed series, were compared with the present study data. Results Concerning the characteristics of lockdown series, mild cases accounted for 14.4, 5...
Background The SARS-CoV-2 infection has widely spread to become the greatest public health challe... more Background The SARS-CoV-2 infection has widely spread to become the greatest public health challenge to date, the COVID-19 pandemic. Different fatality rates among countries are probably due to non-standardized records being carried out by local health authorities. The Spanish case-fatality rate is 11.22%, far higher than those reported in Asia or by other European countries. A multicentre retrospective study of demographic, clinical, laboratory and immunological features of 584 Spanish COVID-19 hospitalized patients and their outcomes was performed. The use of renin-angiotensin system blockers was also analysed as a risk factor. Results In this study, 27.4% of cases presented a mild course, 42.1% a moderate one and for 30.5% of cases, the course was severe. Ages ranged from 18 to 98 (average 63). Almost 60 % (59.8%) of patients were male. Interleukin 6 was higher as severity increased. On the other hand, CD8 lymphocyte count was significantly lower as severity grew and subpopulatio...
The impact of Covid-19 pneumonia caused by SARS-CoV-2 on transplanted populations under chronic i... more The impact of Covid-19 pneumonia caused by SARS-CoV-2 on transplanted populations under chronic immunosuppression seems to be greater than in normal population. Clinical management of the disease, particularly in those patients worsening after a cytokine storm, with or without allograft impairment and using available therapeutic approaches in the absence of specific drugs to fight against the virus, involves a major challenge for physicians. We herein provide evidence of the usefulness of high-dose intravenous immunoglobulin (IVIG) combined with steroid pulses to successfully treat a case of Covid-19 pneumonia in a single-kidney transplanted patient with mechanical ventilation and hemodialysis requirements in the setting of a cytokine storm. A rapid decrease in the serum level of inflammatory cytokines, particularly IL-6, IL-8, TNF-α, MCP-1 and IL-10, as well as of acute-phase reactants such as ferritin, D-dimer and C-reactive protein was observed after the IVIG infusion and methylp...
We herein describe the case of a 60-year-old man who presented clinical and histopathological evi... more We herein describe the case of a 60-year-old man who presented clinical and histopathological evidence of Henoch-Schönlein purpura. Antineutrophil antibodies (ANCA) showed positive results on an enzyme-linked immunosorbent assay and immunofluorescence for anti-myeloperoxidase and anti-proteinase 3 antibodies. Dual positivity for both cytoplasmatic (C-ANCA) and perinuclear (P-ANCA) antineutrophil antibodies has been found previously in a small number of reports, but to our knowledge this is the first time the simultaneous presence of C-ANCA and P-ANCA has been observed in Henoch-Schönlein purpura.
The Journal of Allergy and Clinical Immunology, Feb 1, 2003
inflammatory disease with several etiopathogenic mechanisms. Considering its heterogeneity, it ha... more inflammatory disease with several etiopathogenic mechanisms. Considering its heterogeneity, it has been recently used other designation for this pathology-atopic eczema/dermatitis syndrome (AEDS). The association with food and respiratory allergy is variable among different populations. PURPOSE: To analyze a group of patients referred to our clinic with AEDS in order to evaluate the association of this syndrome with food allergy and respiratory diseases. METHODS: From the total number of first visits to our department during 2000-2001 (n=3436) we selected all patients with a history of AEDS. We analyzed age, gender, presence of food and respiratory allergy and skin prick tests (SPT). RESULTS: We found 193 patients with a mean age of 7,5 years old (1-54 years), female/male ratio 1/1,5. There were 68 patients with isolated AEDS (35,8%). Associated respiratory disease was identified in 113 patients (58,5%) and food allergy in 19 patients (9,8%)-most cases with urticaria/angioedema. SPT were positive to aeroallergens in 64% and to food allergens in 16% of the sample. We found positive SPT results in 47,8% of patients with isolated AEDS, in 84,2% of patients with food allergy and in 92% with respiratory disease. CONCLUSIONS: In contrast to other series, we found a low prevalence of food allergy, in most cases manifested by immediate reactions. More than half of our patients have respiratory allergic diseases associated to a higher prevalence of sensitization to aeroallergens. These results reflect the heterogeneity of AEDS populations.
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Galectin-3 is an animal lectin, formerly named ε-binding protein or Mac-2, which has been describ... more Galectin-3 is an animal lectin, formerly named ε-binding protein or Mac-2, which has been described to play an important role in some inflammatory processes by the implication of different cells and the increase in cell adhesion functions through laminin binding activity. In this work we analyzed the role of galectin-3 in the modulation of Th2 cytokines that have an important role in the development of the inflammatory response. We have found that the addition of galectin-3 to human eosinophils, the eosinophilic cell line EoL-3, PBMC, and an Ag-specific T cell line (CD4+) produced a selective inhibition of IL-5 transcription. No inhibitory effect was found on the IL-4 mRNA transcription rate. The inhibitory effect on IL-5 transcription was reversed by incubation with lactose and using specific Ab against galectin-3. Galectin-3 is able to induce inhibition of the IL-5 released in the supernatants from PBMC stimulated with phorbol 12,13-dibutyrate and anti-CD3. Similar results were ob...
Additional file 1: Supplementary Table 1. Comparison between Phases I and II for clinical and dem... more Additional file 1: Supplementary Table 1. Comparison between Phases I and II for clinical and demographic characteristics.
Background Several antibodies have proven useful in autoimmune diseases, as markers for diagnosis... more Background Several antibodies have proven useful in autoimmune diseases, as markers for diagnosis, prognosis or clinical manifestations. Objectives To evaluate the clinical utility and the various associations of antibodies SSA/Ro52, SSA/Ro60 and SSB/La in LES at a referral hospital in Spain. Methods We retrospectively analise the patterns of all the consecutive samples submitted to the Immunology Unit for antinuclear antibody screening between 2002 and 2012. ANA positive sera for indirect immunofluorescence test (IFI) were analised with a commercial immunoassay (Innolia-ANA update). 323 patients had more than one positive sample for some of the autoantibodies Ro52, Ro60 or La and the pattern of sera for each patient were virtually identical. 113 patients with diagnosis of LES were included in the study. Dependent variables: Each of the possible combinations of results of Ro52, Ro60 and La, ie: 52+/60+/La+; 52+/60+/La-; 52+/60-/La+; 52+/60-/La-; 52-/60+/La+; 52-/60+/La-; 52-/60-/La+. Independent variables: age, sex, diagnostic variables, pattern by IIF, result of the immunoassay, clinical features and laboratory variables. Results Of the 113 patients, 93,8% were women and 6,3% males, aged between 16 and 71 years (mean: 37,7; dev. Typical 13.9) in the moment of the diagnosis. The most frequent pattern were fine speckle, 68 cases (61,3%) and homogeneous 41 (36,6%) The relation of clinical symptoms and the positivity of Ro52, Ro60 and La in their various combinations (Table 1) showed the following statistical association: xerostomia and rheumatoid factor + with Ro52+/Ro60-/La+; arthritis with Ro52+/Ro60+/La-; xerostomia, xerophthalmia, hypergammaglobulinemia and rheumatoid factor with isolated Ro52+; pericardial effusion, subacute cutaneous lesions and hypocomplementemia with Ro52-/Ro60+/La-. In relation to IFI, fine speckeled pattern was positively associated with Ro52+/Ro60+/La+ combination (p 0,003), and La+ isolated (p 0,03). Conclusions Our study found significant relationship between clinical and laboratory manifestations and different patterns of antibodies to Ro52, Ro60 and SSB. The combination of antibodies might be clinically useful because these associations have prognostic and therapeutic implications. Would be worthwhile to explore its usefulness in other ethnic groups. Disclosure of Interest None Declared
Esophageal benign tumors appear very infrequently and, in contrast to malignant ones, the majorit... more Esophageal benign tumors appear very infrequently and, in contrast to malignant ones, the majority are asymptomatic. Leiomyomas, papillomas, and adenomas are the most frequent among them; angiomas, fibromas, schwannomas (neurogenic tumors), lipomas, or glandular retention cysts are much less frequent. Most esophageal polyps are fibrovascular ones (1), are located in the proximal third of the esophagus, and frequently present with symptoms of regurgitation (2). An inflammatory fibroid polyp is an entity that was first described by Vanek as a "gastric submucosal granuloma with eosinophilia" (3). It has variously been called eosinophilic granuloma, granuloblastoma, gastric neurofibroma, xanthofibroma, etc (4). This condition is very seldom found, and its most frequent gastrointestinal localization is the stomach, the small intestine and, less frequently, the colon. However, some cases have been reported of esophageal (5-7), duodenal (8), and rectal (9) localization. We present the case of a patient with an esophageal inflammatory fibroid polyp who showed neither dysphagia nor reflux symptoms and we note the endoscopic and radiologic aspects of the polyp. CASE REPORT A male patient, age 60 years, was sent to our center for surgical treatment of a possible esophageal neoplasm. He usually smoked 20 cigarettes a day and was a moderate alcohol consumer. His personal history showed that he had been submitted to surgery three years before because Manuscript
Background The amino-acid and nucleotide sequence of Ole e 1 (the major antigen of olive pollen) ... more Background The amino-acid and nucleotide sequence of Ole e 1 (the major antigen of olive pollen) has been described and the IgE antibody response to this major allergen was associated with DR7/DQ2 antigens. With this previous data we try to define the T-cell epitopes implicated in Ole e 1 reactivity. Objectives To study the recognition of T cells (derived from allergic and non-allergic Ole e 1 patients) to Ole e 1 synthetic peptides in order to define immunodominant T-cell epitopes. Methods We have compared the proliferative response of the peripheral blood mononuclear cells from Ole e 1 sensitized patients vs. non-sensitized controls, induced by 14 Ole e 1 synthetic peptides. Thirty subjects were classified in two groups: group 1 (nonresponders against Ole e 1, n ¼ 16) and group 2 (Ole e 1 responders, n ¼ 14), according to their clinical parameters and the presence or not in their sera of the significant Ole e 1 IgE antibody levels. Results Our results shown that it is possible to find T cells reactive to Ole e 1 peptides in patients with and without significant levels of Ole e 1 IgE antibodies. However, the percentage of response was higher in patients with IgE antibodies 71.4% vs 25%), and the recognition profile was different: the control group showed a broad reactivity pattern, in contrast, the response by the 'Ole e 1 responders' group was mainly directed against three peptides of the carboxi-terminal region, peptides 10 (91-102), 12 (109-120) and 13 (119-130), with a response frequency of 35.7, 28.5 and 28.5%, respectively. By direct and inhibition test no antibody response was found against the synthetic peptides. Conclusions Our data suggest that the regions between 91 and 102 and 109-130 aminoacids on the Ole e 1 molecule are immunodominant T-cell epitopes. These epitopes are not recognized by IgE antibodies.
Expression of B7 molecules plays an important role in antigen presentation and T cell proliferati... more Expression of B7 molecules plays an important role in antigen presentation and T cell proliferation. Although eosinophils (co.,,) have been reported to express HLA-DR on activation and to present antigens to T cells, little is known about their co-stimulalory properties. The purpose of this stmlv was to determine whelher eos express B7 molecules and io assess their role in antigen presentation. Four normal volunteers and one hyperlgE syndrome patient were evaluated. Eos prep'lrations of 97-99% purity were obtained by immunomagnelic separation with anti-CD16, cultured with It.3, stained directly with HLA-DR, B7-2 or B7-1 antibodies nnd analyzed by FACS. No expression of I-II.A-DF,, B7-2 or BT-I was noted on freshly isolated cos. Bv contrast, 72-96 hours later, IL-3 induced the expression of HLA-DR in 70 + 15% and B7-2 in 25 + 8 % of the cos, but not B7-1. Furthermore, Eos supported T cell prolifenuion to the super:mtigen TSST-I. The proliferation was inhibited 50% by CTLA4Ig and anti-B7-2, but not by an isotype control. We conclude that hurnan cos express 137-2 which provides co-stinmlator 3, signals to T cells for activation and proliferation. Studies are in progress to elucidate the mechanisms of T cell activation by eos via the B7-2 pathway.
Authors: Gallardo S.; del Pozo V.; Cardaba B.; Jurado A.; Cortegano I.; del Amo A.; Arrieta I.; P... more Authors: Gallardo S.; del Pozo V.; Cardaba B.; Jurado A.; Cortegano I.; del Amo A.; Arrieta I.; Palomino P.; Lahoz C. ... This article is hosted on another website. ... You may be required to register, activate a subscription or purchase the article before you can obtain the full text.
Galectin-3 is an animal lectin, formerly named ⑀-binding protein or Mac-2, which has been describ... more Galectin-3 is an animal lectin, formerly named ⑀-binding protein or Mac-2, which has been described to play an important role in some inflammatory processes by the implication of different cells and the increase in cell adhesion functions through laminin binding activity. In this work we analyzed the role of galectin-3 in the modulation of Th2 cytokines that have an important role in the development of the inflammatory response. We have found that the addition of galectin-3 to human eosinophils, the eosinophilic cell line EoL-3, PBMC, and an Ag-specific T cell line (CD4 ؉) produced a selective inhibition of IL-5 transcription. No inhibitory effect was found on the IL-4 mRNA transcription rate. The inhibitory effect on IL-5 transcription was reversed by incubation with lactose and using specific Ab against galectin-3. Galectin-3 is able to induce inhibition of the IL-5 released in the supernatants from PBMC stimulated with phorbol 12,13-dibutyrate and anti-CD3. Similar results were obtained when a T-specific cell line was stimulated with Ag. Also, EoL-3 stimulated with anti-CD32 produced IL-5 protein, the synthesis of which was partially inhibited by galectin-3. The present results demonstrate that galectin-3 induces a selective down-regulation of IL-5 expression in different cell types, opening important new possibilities in the regulation of the allergic reactions.
AUTHORS. Aurora Jurado1,2, María C. Martín1,3, Cristina Abad-Molina4, Antonio Orduña4, Alba Martí... more AUTHORS. Aurora Jurado1,2, María C. Martín1,3, Cristina Abad-Molina4, Antonio Orduña4, Alba Martínez5, Esther Ocaña5, Oscar Yarce2, Ana M. Navas2 , Antonio Trujillo2, Luis Fernández6, Esther Vergara6 , Beatriz Rodríguez7, Bibiana Quirant8, Eva Martínez8, Manuel Hernández9, Janire Perurena-Prieto9, Juana Gil10, Sergi Cantenys10, Gema GonzálezMartínez11, María T. Martínez-Saavedra11, Ricardo Rojo12, Francisco M. Marco13, Sergio Mora13 Jesús Ontañón14, Marcos López-Hoyos15, Gonzalo Ocejo-Vinyals15,Josefa Melero16, Marta Aguilar16, Delia Almeida17, Silvia Medina17, María C. Vegas18, Yesenia Jiménez18, Álvaro Prada19, David Monzón19, Francisco Boix20, Vanesa Cunill21, Juan Molina2 1These authors contributed equally to this manuscript. Affiliations.
OBJECTIVES ANA are the most extensively used test for the diagnosis of systemic autoimmune diseas... more OBJECTIVES ANA are the most extensively used test for the diagnosis of systemic autoimmune diseases. However, testing by indirect immunofluorescence assays (IIFAs) on HEp-2 cells, the gold standard test, is time-consuming and needs expertise. Thus there is a trend to replace it with other automated solid-phase assays directed against specific ANA. Nonetheless, the Hep-2 cell is an autoantigen array and ANA have been classified into 29 types, some of them with no clear association with a specificity to be detected. It is especially in these uncommon patterns where no clinical relationship is found and no antigenic specificity is detected. Here we retrospectively collected clinical data from patients with confirmed uncommon HEp-2 IIFA patterns to search for an associated clinical condition. METHODS We conducted an observational retrospective study including 608 patients with organ-specific and non-organ-specific autoimmune diseases (OSADs and NOSADs, respectively) with a confirmed rar...
Background One hundred fifty million contagions, more than 3 million deaths and little more than ... more Background One hundred fifty million contagions, more than 3 million deaths and little more than 1 year of COVID-19 have changed our lives and our health management systems forever. Ageing is known to be one of the significant determinants for COVID-19 severity. Two main reasons underlie this: immunosenescence and age correlation with main COVID-19 comorbidities such as hypertension or dyslipidaemia. This study has two aims. The first is to obtain cut-off points for laboratory parameters that can help us in clinical decision-making. The second one is to analyse the effect of pandemic lockdown on epidemiological, clinical, and laboratory parameters concerning the severity of the COVID-19. For these purposes, 257 of SARSCoV2 inpatients during pandemic confinement were included in this study. Moreover, 584 case records from a previously analysed series, were compared with the present study data. Results Concerning the characteristics of lockdown series, mild cases accounted for 14.4, 5...
Background The SARS-CoV-2 infection has widely spread to become the greatest public health challe... more Background The SARS-CoV-2 infection has widely spread to become the greatest public health challenge to date, the COVID-19 pandemic. Different fatality rates among countries are probably due to non-standardized records being carried out by local health authorities. The Spanish case-fatality rate is 11.22%, far higher than those reported in Asia or by other European countries. A multicentre retrospective study of demographic, clinical, laboratory and immunological features of 584 Spanish COVID-19 hospitalized patients and their outcomes was performed. The use of renin-angiotensin system blockers was also analysed as a risk factor. Results In this study, 27.4% of cases presented a mild course, 42.1% a moderate one and for 30.5% of cases, the course was severe. Ages ranged from 18 to 98 (average 63). Almost 60 % (59.8%) of patients were male. Interleukin 6 was higher as severity increased. On the other hand, CD8 lymphocyte count was significantly lower as severity grew and subpopulatio...
The impact of Covid-19 pneumonia caused by SARS-CoV-2 on transplanted populations under chronic i... more The impact of Covid-19 pneumonia caused by SARS-CoV-2 on transplanted populations under chronic immunosuppression seems to be greater than in normal population. Clinical management of the disease, particularly in those patients worsening after a cytokine storm, with or without allograft impairment and using available therapeutic approaches in the absence of specific drugs to fight against the virus, involves a major challenge for physicians. We herein provide evidence of the usefulness of high-dose intravenous immunoglobulin (IVIG) combined with steroid pulses to successfully treat a case of Covid-19 pneumonia in a single-kidney transplanted patient with mechanical ventilation and hemodialysis requirements in the setting of a cytokine storm. A rapid decrease in the serum level of inflammatory cytokines, particularly IL-6, IL-8, TNF-α, MCP-1 and IL-10, as well as of acute-phase reactants such as ferritin, D-dimer and C-reactive protein was observed after the IVIG infusion and methylp...
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