Filamins are dimeric actin-binding proteins participating in the organization of the actin-based ... more Filamins are dimeric actin-binding proteins participating in the organization of the actin-based cytoskeleton. Their modular domain organization is made up of an N-terminal actin-binding domain composed of two CH domains followed by flexible rod regions that consist of 24 Ig-like domains. Homology modeling was used to model human filamin using Modeller 9v5. The resulting model assessed by Verify 3D and PROCHECK showed that the final model is reliable. The conformational disorder prediction of human filamin residues were also mapped on the validated structure of human filamin. Prediction of protein disorder in filamin structures will help understand its role in function.
Maize is an important food and feed crop worldwide. Phytic acid (PA), in maize kernel, is an anti... more Maize is an important food and feed crop worldwide. Phytic acid (PA), in maize kernel, is an antinutritional factor. PA chelates mineral cations and causes mineral deficiency in humans and phosphorous deficiency in animals. The undigested PA excreted by monogastric animals causes phosphorous eutrophication. Therefore, development of low-phytate maize is indispensable. The low-phytate locus (lpa2 allele) has been transferred from low-phytate mutant line 'EC 659418' into an elite inbred UMI 395 through marker-assisted backcross breeding (MABB). The MABB involved three backcrosses followed by two selfing steps, including 'foreground selection', that is, selecting lines with lpa2 allele with the help of a codominant SSR marker 'umc2230' and 'background selection', that is, selecting plants having genetic background similar to that of the recurrent parent using 50 codominant SSR markers. Two low-phytate lpa2 lines with genome similar (>90% similarity) to that of recurrent parent have been identified. These lines can be used as parent in future hybridization programmes for obtaining low-phytate high-yielding maize hybrids.
Filamins are dimeric actin-binding proteins participating in the organization of the actin-based ... more Filamins are dimeric actin-binding proteins participating in the organization of the actin-based cytoskeleton. Their modular domain organization is made up of an N-terminal actin-binding domain composed of two CH domains followed by flexible rod regions that consist of 24 Ig-like domains. Homology modeling was used to model human filamin using Modeller 9v5. The resulting model assessed by Verify 3D and PROCHECK showed that the final model is reliable. The conformational disorder prediction of human filamin residues were also mapped on the validated structure of human filamin. Prediction of protein disorder in filamin structures will help understand its role in function.
Maize is an important food and feed crop worldwide. Phytic acid (PA), in maize kernel, is an anti... more Maize is an important food and feed crop worldwide. Phytic acid (PA), in maize kernel, is an antinutritional factor. PA chelates mineral cations and causes mineral deficiency in humans and phosphorous deficiency in animals. The undigested PA excreted by monogastric animals causes phosphorous eutrophication. Therefore, development of low-phytate maize is indispensable. The low-phytate locus (lpa2 allele) has been transferred from low-phytate mutant line 'EC 659418' into an elite inbred UMI 395 through marker-assisted backcross breeding (MABB). The MABB involved three backcrosses followed by two selfing steps, including 'foreground selection', that is, selecting lines with lpa2 allele with the help of a codominant SSR marker 'umc2230' and 'background selection', that is, selecting plants having genetic background similar to that of the recurrent parent using 50 codominant SSR markers. Two low-phytate lpa2 lines with genome similar (>90% similarity) to that of recurrent parent have been identified. These lines can be used as parent in future hybridization programmes for obtaining low-phytate high-yielding maize hybrids.
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Papers by Suresh Kumar