Papers by Panlop Chakkavittumrong
2022 44th Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC)
Journal of the European Academy of Dermatology and Venereology
Research in Pharmaceutical Sciences, 2022
Artificial intelligence(AI) has been reported in high potential in classification of pigmented sk... more Artificial intelligence(AI) has been reported in high potential in classification of pigmented skin lesions(PSLs) under experimental conditions recently. Thai researchers team has trained the first AI which enable to diagnose PSLs in Thailand. AI showed high sensitivity, specificity, and accuracy in diagnosis up to 80-90%. Although the computer diagnosis of PSLs is accurate under experimental conditions, the practical value of automated diagnostic tool compared to standard is currently unknown. This study aimed to validate the diagnosis performances of AI assisted in PSLs diagnosis compared to Board-certified dermatologist using dermoscopic images. Index Terms Artificial intelligence, dermoscopy, pigmented skin lesions
Journal of Cosmetic Dermatology
Polymers
P. frutescens seed oil and M. oleifera seed oil consist of fatty acids and sterols that are benef... more P. frutescens seed oil and M. oleifera seed oil consist of fatty acids and sterols that are beneficial for skin. Mixing of these oils at 1:1 ratio has shown to increase antioxidant activity of oils. This study aims to formulate emulgels containing microemulsions of P. frutescens seed oil, M. oleifera seed oil, and mixed P. frutescens and M. oleifera seed oils. The chemical constituents of P. frutescens seed oil, M. oleifera seed oil, and mixed seed oil are analyzed by gas chromatography/mass spectrometry (GC/MS). The microemulsions are formulated by a phase titration method and characterized for the droplet size, polydispersity index, and zeta potential value using a dynamic light scattering technique. The physical and chemical stability of the microemulsions are investigated using a rheometer and UV-Visible spectrophotometer, respectively. The safety of microemulsion is evaluated on PBMC and human subjects. Emulgels containing three different types of microemulsion are formulated. ...
Scientific Reports, 2022
Androgenic alopecia is a common type of hair loss, usually caused by testosterone metabolism gene... more Androgenic alopecia is a common type of hair loss, usually caused by testosterone metabolism generating dihydrotestosterone and hair follicular micro-inflammation. These processes induce dermal papilla cells to undergo apoptosis. Currently approved effective medications for alopecia are Finasteride, an oral 5α-reductase inhibitor, Minoxidil, a topical hair growth promoter, and Diclofenac, an anti-inflammatory agent, all of which, however, have several adverse side effects. In our study, we showed the bioactivity of Acanthus ebracteatus Vahl. (AE) extract performed by 95% ethanol, and verbascoside (VB), a biomarker of AE extract. Both AE extract and VB were studied for their effects on dermal papilla cell viability and the cell cycle by using MTT assay and flow cytometry. The effect of an anti-inflammatory activity of AE extract and VB on IL-1β, NO, and TNF-α, released from LPS induced RAW 264.7 cells, and IL-1α and IL-6 released from irradiated dermal papilla cells were detected usi...
The Journal of clinical and aesthetic dermatology, Jun 1, 2021
This study was a double-blinded, randomized, controlled, split-face trial. Neither the investigat... more This study was a double-blinded, randomized, controlled, split-face trial. Neither the investigator nor the participants knew which drugs were administered. A
Acne is a common chronic inflammatory disease of pilosebaceous units. Acne has a complex physiolo... more Acne is a common chronic inflammatory disease of pilosebaceous units. Acne has a complex physiological basis involving four main factors; excess sebum production influenced by androgenic hormone, excess keratinocytes production, increase bacterial Propionibacterium acnes proliferation and inflammation. The common complication of acne are psychosocial effects and post inflammatory hyperpigmentation. To understand the role of inflammation involves with acne vulgaris and hyperpigmentation formation may constitute new treatment possibilities for acne vulgaris. Several previous studies show the involvement of COX and LOX pathway in acne pathogenesis. Since, 1% diclofenac gel blocks the activity of COX and LOX pathway, a clinical study has been conducted to evaluate the efficacy and safety of 1% diclofenac gel and 2.5% benzoyl peroxide gel for 12 weeks in patients with mild to moderate acne vulgaris.
Introduction: Testing promoter irritation and allergic reaction to the skin's immune is absol... more Introduction: Testing promoter irritation and allergic reaction to the skin's immune is absolutely necessary for safety testing of the plant extract before formulation. Piper chaba fruits (long pepper) is a component in multi-drug formulations of Thai traditional medicine. It is used for topical pain relief, anti-inflammation of the muscles. In pharmacological studies, it could be used as anti-inflammatory and reducing pain. The extract is not toxic in either sub-acute and chronic toxicity test. Thus, study of safety of Piper chaba extract regarding irritation and immunomodulatory allergy on skin is necessary. These data are used for formulation of topical preparation in the future. Objective was to investigate on irritant reaction and immunomodulatory allergy of the ethanolic extract of Piper chaba fruit (long pepper) on skin in healthy volunteers. Method: Clinical safety was studied of ethanolic extract of Piper chaba fruit (long pepper). The allergic reaction and irritation r...
Surgical and Cosmetic Dermatology, 2020
Comparação clínica entre peróxido de benzoília tópico 2,5% e diclofenaco gel 1% versus peróxido d... more Comparação clínica entre peróxido de benzoília tópico 2,5% e diclofenaco gel 1% versus peróxido de benzoília 2,5% e placebo no tratamento da acne vulgar facial leve à moderada Clinical comparison between topical benzoyl peroxide 2.5% and diclofenac gel 1% versus benzoyl peroxide 2.5% and placebo in the treatment of mild to moderate facial acne vulgaris
Allergy, Asthma & Immunology Research, 2021
Propose: The purpose of this study was to investigate panels of enzyme-linked immunospot assays (... more Propose: The purpose of this study was to investigate panels of enzyme-linked immunospot assays (ELISpot) to detect drug-specific mediator releasing cells for confirming culprit drugs in severe cutaneous adverse reactions (SCARs). Methods: Frequencies of drug-induced interleukin-22 (IL-22)-, interferon-gamma (IFN-γ)-, and granzyme-B (GrB)-releasing cells were measured by incubating peripheral blood mononuclear cells (PBMCs) from SCAR patients with the culprit drugs. Potential immunoadjuvants were supplemented to enhance drug-induced mediator responses. Results: Twenty-seven patients, including 9 acute generalized exanthematous pustulosis (AGEP), 10 drug reactions with eosinophilia and systemic symptoms, and 8 Stevens-Johnson syndrome and toxic epidermal necrolysis (SJS/TEN) were recruited. The average frequencies of drug-induced IL-22-, IFN-γ-, and GrB-releasing cells were 35.5±16.3, 33.0±7.1, and 164.8±43.1 cells/million PBMCs, respectively. The sensitivity of combined IFN-γ/IL-22/GrB ELISpot was higher than that of IFN-γ ELISpot alone for culprit drug detection in all SCAR subjects (77.8% vs 51.9%, P < 0.01). The measurement of drug-induced IL-22-and IFN-γ
Molecules, 2020
The health benefits of the Aquilaria crassna Pierre ex Lecomte leaf extract (AE) make it very use... more The health benefits of the Aquilaria crassna Pierre ex Lecomte leaf extract (AE) make it very useful as an ingredient in food and pharmaceutical products. Iriflophenone 3,5-C-β-d-diglucoside (1), iriflophenone 3-C-β-d-glucoside (2) and mangiferin (3) are bioactive compounds of AE. We assessed the stability of AE by investigating the thermal degradation kinetics and shelf-life (t90%) of compounds 1, 2 and 3 using Arrhenius plot models and studied their pH-rate profiles. The results demonstrate that 1 and 2 were degraded, following a first-order kinetic reaction. The degradation of 3 followed first-order reaction kinetics when present in a solution and second-order reaction kinetics in the dried powder form of the extract. According to the first-order kinetic model, the predicted shelf-life (t90%) of the extract at 25 °C in dried form for compound 1 was 989 days with activation energy 129.86 kJ·mol−1, and for 2 it was 248 days with activation energy 110.57 kJ·mol−1, while in the extra...
Journal of Cosmetic Dermatology, 2020
Acne scarring is a cosmetic problem that may affect 95% of acne patients and has a significant ne... more Acne scarring is a cosmetic problem that may affect 95% of acne patients and has a significant negative effect on the quality of life (QOL) of young adults. 1,2 Eighty to ninety percent of acne scars patients have atrophic scars compared to a minority who show hypertrophic scars and keloids. 3 Unfortunately, there has been no standard treatment option for the treatment of acne scars. Various therapeutic options have been described with variable clinical outcomes and complications. 4
Journal of the European Academy of Dermatology and Venereology, 2018
Background: The lymphocyte transformation test (LTT) is a standard laboratory method to identify ... more Background: The lymphocyte transformation test (LTT) is a standard laboratory method to identify culprit drugs in patients with a history of drug-induced non-immediate hypersensitivity and is mainly performed during the recovery phase. The measurement of drug-specific interferon-γ (IFN-γ)-releasing cells has been introduced to confirm culprit drugs, even during the acute phase of drug allergy. Objectives: This study aimed to evaluate the capability of the enzyme-linked immunospot assay (ELISpot) to detect drug-specific IFN-γ-releasing cells during the acute phase and the capability of LTT to identify culprit drugs during the recovery phase in patients presenting with severe cutaneous adverse reactions (SCARs). Methods: Peripheral blood mononuclear cells (PBMCs) from 23 SCAR patients were collected during the acute and recovery phases and assayed for drug-specific IFN-γ-releasing cells and lymphocyte proliferation, respectively. Results: Drug-specific IFN-γ releasing cells were detectable in 73.9% of SCAR subjects (55.6% and 85.7% in patients who were and were not taking systemic steroids, respectively), whereas LTT results were positive in 52.2% of SCAR subjects. The frequencies of drug-specific IFN-γ-releasing cells were significantly higher in patients with positive LTT than in those with negative LTT (260.1 ± 110.0 and 46.6
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Papers by Panlop Chakkavittumrong