Papers by Nurshahieda Mohamad
Scientific reports, Mar 7, 2024
Vibrio parahaemolyticus is a gram-negative facultative anaerobic bacterium implicated as the caus... more Vibrio parahaemolyticus is a gram-negative facultative anaerobic bacterium implicated as the causative agent of several shrimp diseases. As part of the effort to provide biocontrol and costeffective treatments, this research was designed to elucidate the effect of Morinda citrifolia fruit extract on the immunity of Penaeus vannamei postlarvae (PL) to V. parahaemolyticus. The methanol extract of M. citrifolia was vacuum evaporated, and the bioactive compounds were detected using gas chromatography-mass spectrometry (GC-MS). Thereafter, P. vannamei PL diets were supplemented with M. citrifolia at different concentrations (0, 10, 20, 30, 40, and 50 mg/g) and administered for 30 days before 24 h of exposure to the bacterium V. parahaemolyticus. A total of 45 bioactive compounds were detected in the methanol extract of M. citrifolia, with cyclononasiloxane and octadecamethyl being the most abundant. The survival of P. vannamei PLs fed the extract supplement was better than that of the control group (7.1-26.7% survival greater than that of the control group) following V. parahaemolyticus infection. Shrimp fed 50 mg/g M. citrifolia had the highest recorded survival. The activities of digestive and antioxidant enzymes as well as hepatopancreatic cells were significantly reduced, except for those of lipase and hepatopancreatic E-cells, which increased following challenge with V. parahaemolyticus. Histological assessment of the hepatopancreas cells revealed reduced cell degeneration following the administration of the plant extracts (expecially those fed 50 mg/g M. citrifolia) compared to that in the control group. Therefore, the enhanced immunity against V. parahaemolyticus infection in P. vannamei could be associated with the improved hepatopancreas health associated with M. citrifolia fruit extract supplementation.
Aquaculture Reports, Nov 1, 2020
Aquaculture Reports, 2020
Abstract The present study aims to isolate, identify, and evaluate potential probiotic, Lactic Ac... more Abstract The present study aims to isolate, identify, and evaluate potential probiotic, Lactic Acid Bacteria (LAB) from the gut and hepatopancreas of 10 healthy wild giant freshwater prawn, Macrobrachium rosenbergii. The samples were cultivated using a modified De Man, Rogosa and Sharpe (MRS) agar supplemented with 2% of CaCO3 (w/v). Bacterial colonies with strong clear zone were selected for further tests. From these, 17 isolates of LAB showed non-spore forming, homofermentative characteristics, Gram-positive, rod-shaped, non-motile bacteria and absences of catalase enzymes. The LAB was further identified using API 50 CH kit and 16S rRNA. The isolates LAB also showed tolerance to a variety of environmental conditions like pH, temperature, salt, bile and hemolysis activity in in vitro condition. Based on the results, two isolates LAB were identified as Lactobacillus plantarum and Entercoccus faecalis were suggested as potential probiotic candidates from giant freshwater prawn for Aquaculture industries.
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Papers by Nurshahieda Mohamad