Spectrochimica Acta Part A-molecular and Biomolecular Spectroscopy, 2009
Mode of interactions of three flavonoids [morin (M), quercetin (Q), and rutin (R)] with chicken b... more Mode of interactions of three flavonoids [morin (M), quercetin (Q), and rutin (R)] with chicken blood ds.DNA (ck.DNA) has been investigated spectrophotometrically at different temperatures including body temperature (310 K) and at two physiological pH values, i.e. 7.4 (human blood pH) and 4.7 (stomach pH). The binding constants, K f , evaluated using Benesi-Hildebrand equation showed that the flavonoids bind effectively through intercalation at both pH values and body temperature. Quercetin, somehow, showed greater binding capabilities with DNA. The free energies of flavonoid-DNA complexes indicated the spontaneity of their binding. The order of binding constants of three flavonoids at both pH values were found to be K f(Q) > K f(R) > K f(M) and at 310 K.
Spectrochimica Acta Part A-molecular and Biomolecular Spectroscopy, 2011
Interactional studies of new flavonoid derivatives (Fl) with chicken blood ds.DNA were investigat... more Interactional studies of new flavonoid derivatives (Fl) with chicken blood ds.DNA were investigated spectrophotometrically in DMSO–H2O (9:1 v/v) at various temperatures. Spectral parameters suggest considerable binding between the flavonoid derivatives studied and ds.DNA. The binding constant values lie in the enhanced-binding range. Thermodynamic parameters obtained from UV studies also point to strong spontaneous binding of Fl with ds.DNA. Viscometric studies complimented the UV results where a small linear increase in relative viscosity of the DNA solution was observed with added optimal flavonoid concentration. An overall mixed mode of interaction (intercalative plus groove binding) is proposed between DNA and flavonoids. Conclusively, investigated flavonoid derivatives are found to be strong DNA binders and seem to be promising drug candidates like their natural analogues.► New flavonoid derivatives were investigated spectrophotometrically to interact strongly with ds.DNA. ► Thermodynamic parameters obtained from UV studies also point to spontaneous binding of all flavonoid derivatives to ds.DNA. ► These flavonoids like their natural analogues seem to be promising drug candidates according to UV–vis results complemented by viscosity data and structural parameters.
Spectrochimica Acta Part A-molecular and Biomolecular Spectroscopy, 2009
Mode of interactions of three flavonoids [morin (M), quercetin (Q), and rutin (R)] with chicken b... more Mode of interactions of three flavonoids [morin (M), quercetin (Q), and rutin (R)] with chicken blood ds.DNA (ck.DNA) has been investigated spectrophotometrically at different temperatures including body temperature (310 K) and at two physiological pH values, i.e. 7.4 (human blood pH) and 4.7 (stomach pH). The binding constants, K f , evaluated using Benesi-Hildebrand equation showed that the flavonoids bind effectively through intercalation at both pH values and body temperature. Quercetin, somehow, showed greater binding capabilities with DNA. The free energies of flavonoid-DNA complexes indicated the spontaneity of their binding. The order of binding constants of three flavonoids at both pH values were found to be K f(Q) > K f(R) > K f(M) and at 310 K.
Spectrochimica Acta Part A-molecular and Biomolecular Spectroscopy, 2011
Interactional studies of new flavonoid derivatives (Fl) with chicken blood ds.DNA were investigat... more Interactional studies of new flavonoid derivatives (Fl) with chicken blood ds.DNA were investigated spectrophotometrically in DMSO–H2O (9:1 v/v) at various temperatures. Spectral parameters suggest considerable binding between the flavonoid derivatives studied and ds.DNA. The binding constant values lie in the enhanced-binding range. Thermodynamic parameters obtained from UV studies also point to strong spontaneous binding of Fl with ds.DNA. Viscometric studies complimented the UV results where a small linear increase in relative viscosity of the DNA solution was observed with added optimal flavonoid concentration. An overall mixed mode of interaction (intercalative plus groove binding) is proposed between DNA and flavonoids. Conclusively, investigated flavonoid derivatives are found to be strong DNA binders and seem to be promising drug candidates like their natural analogues.► New flavonoid derivatives were investigated spectrophotometrically to interact strongly with ds.DNA. ► Thermodynamic parameters obtained from UV studies also point to spontaneous binding of all flavonoid derivatives to ds.DNA. ► These flavonoids like their natural analogues seem to be promising drug candidates according to UV–vis results complemented by viscosity data and structural parameters.
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