Papers by Shahin Hassanpour
Physiology and Pharmacology, Dec 10, 2015
Introduction: Morphine withdrawal syndrome is mediated via several central and peripheral neurolo... more Introduction: Morphine withdrawal syndrome is mediated via several central and peripheral neurological pathways. In the present study we investigated the role of Nmethyl-D aspartic acid (NMDA) glutamate receptor on naloxone-induced withdrawal syndrome in morphine-conditioned mice. Materials and Methods: We designed two separate experiments. In experiment one, 30 male NMRI mice were divided into 5 groups, pretreated with memantine (0.1, 1 and 5 mg/kg; I.P.) followed by morphine-dependence period for 3 days. In the other experiment, 48 male NMRI mice distributed into 8 groups, pretreated with intraaccumbens (IAc) memantine (1 and 5 µg/animal) within the right, left and both side of nucleus accumbens (RNAcc, LNAcc and BNAcc) followed by I.P. morphinedependence (3 days). On day 4, in both experiments, morphine was injected into mice, followed by naloxone. Then naloxone-induced total jumping count, jump height and defecation in morphine-conditioned mice were recorded for 30 min. Results: Pre-treatment by I.P. injection of memantine significantly attenuated naloxone precipitated jumping count/30 min, jumping height (mm) and fecal material output in morphine dependent mice (P<0.05). Also, IAC pretreatment with memantine in LNAcc, RNAcc and BNAcc significantly declined the effect of I.P. injection of naloxone on total jumping count and jumping height (P<0.05), pretreatment within memantine in LNAcc, RNAcc and BNAcc had no effect on defecation (P>0.05). Conclusion: These findings indicated asymmetric involvement of central and peripheral NMDA glutamate receptors in withdrawal syndrome development in morphine-dependent mice.
Iranian Journal of Veterinary Medicine
Background: Parturition depression is an important physiological problem, and several attempts ha... more Background: Parturition depression is an important physiological problem, and several attempts have been made to ascertain this physiological phenomenon. Natural monoterpenes like α-pinene have numerous beneficial properties, but no studies have been done on their antidepressant potential in postpartum animals. Objectives: This study aimed to determine the effects of prenatal administration of α-pinene on the antidepressant-like behavior of mice following delivery. Methods: Pregnant female mice were randomly assigned into four groups. In the control group, the animals were injected with saline on their 5, 8, 11, 14, and 17 gestation days (GD). In groups 2 to 4, pregnant female mice were injected with α-pinene (0.1, 0.5, and 1 mg/kg, respectively) at GD 5, 8, 11, 14, and 17. On day 2 postpartum, open field test (OFT), rotarod, forced swimming test (FST), and tail suspension test (TST) were used to evaluate the antidepressant activity of α-pinene in mice. Also, serum samples were take...
Physiology and Pharmacology, 2015
Introduction: Morphine withdrawal syndrome is mediated via several central and peripheral neurolo... more Introduction: Morphine withdrawal syndrome is mediated via several central and peripheral neurological pathways. In the present study we investigated the role of N- methyl-D aspartic acid (NMDA) glutamate receptor on naloxone-induced withdrawal syndrome in morphine-conditioned mice. Materials and Methods: We designed two separate experiments. In experiment one, 30 male NMRI mice were divided into 5 groups, pretreated with memantine (0.1, 1 and 5 mg/kg; I.P.) followed by morphine-dependence period for 3 days. In the other experiment, 48 male NMRI mice distributed into 8 groups, pretreated with intra- accumbens (IAc) memantine (1 and 5 µg/animal) within the right, left and both side of nucleus accumbens (RNAcc, LNAcc and BNAcc) followed by I.P. morphine- dependence (3 days). On day 4, in both experiments, morphine was injected into mice, followed by naloxone. Then naloxone-induced total jumping count, jump height and defecation in morphine-conditioned mice were recorded for 30 min. Re...
Behavioural Brain Research, 2020
Pain is a physiological response which is mediated via the central and peripheral nervous system.... more Pain is a physiological response which is mediated via the central and peripheral nervous system. Betaine, is a methyl glycine derivative and a commonly used nutrient supplement. The main purpose of the current paper is to determine the possible anti-nociceptive and antioxidant activity and sedative effect of betaine in mice. Adult male albino mice were divided into two categories, formalin and writhing tests. In the formalin test, mice were injected with betaine (10, 20 and 30 mg/kg) or morphine (5 mg/kg). For co-injections mice received betaine (30 mg/kg) + naloxone (2 mg/kg) or atropine (1 mg/kg), chlorpheniramine (20 mg/kg), flumazenil (5 mg/kg), cimetidine (12.5 mg/kg) and cyproheptadine (4 mg/kg). Then the formalin test was done and paw licking time was determined. In the writhing test, injections were the same but the animals were injected with acetic acid (0.6 %) and the percentage of writhing inhibition was recorded. At the end of the study, blood antioxidant levels were determined. According to the results, betaine reduced the pain response in a dose-dependent manner. Co-administration of the naloxone + betaine or flumazenil + betaine significantly decreased the anti-nociceptive effect of betaine on the licking and biting time of the injected paw and inhibited the number of writhing movements. Betaine decreased malondialdehyde (MDA) and improved superoxide dismutase (SOD) and glutathione peroxidase (GPx) levels in formalin receiving mice. No adverse locomotion and sedation effect were observed in betaine-treated mice. These findings suggest that betaine has anti-nociceptive and antioxidant activity in mice, and its anti-nociceptive role interacts with opioidergic and GABA receptors.
conference.khuisf.ac.ir
Background: Recently, agriculture by pass products has been evaluated as sources of antimicrobial... more Background: Recently, agriculture by pass products has been evaluated as sources of antimicrobial agents with efficacies against a variety of microorganisms. Tannins are the secondary metabolites and can found in various species of shrubs, trees and fruits. Previous researchers have been proved which tannins have different effects e.g. nutritional, anthelmintic, immunity response, antibacterial, antimicrobial and antioxidant on human and animals. Many herb and spice extracts contained high levels of phenolics and exhibited antibacterial activity against bacteria. Gram-positive bacteria were generally more sensitive to the tested extracts than Gram-negative ones. Pomegranate (Punica granatum L.) is native to the region from northern India to Iran but it is also widely cultivated now in parts of Southwest America, Mexico and Africa. Pharmacological effects of pomegranate represent a long history and have been mentioned in the Greek and Egyptian documents. Objective: The aim of review was to investigate the potential effects of antibacterial activities of pomegranate peel extract (rind), seed extract, juice and whole fruit on bacteria and fungi. Conclusion: Food poisoning is still a concern for both consumers and the food industry despite the use of various preservation methods. Food processors, food safety researchers and regulatory agencies are continuously concerned with the high and growing number of illness outbreaks caused by some pathogenic and spoilage microorganisms in foods. The increasing antibiotic resistance of some pathogens that are associated with foodborne illness is another concern. This review suggested consumption or using tannin-containing fruits especially Pomegranate, could cure or prevent various illnesses by mentioned effects of side effects.
Comparative Clinical Pathology
Neuroscience Letters, Aug 1, 2023
Neuroscience Letters, Apr 1, 2022
International Journal of Developmental Neuroscience, Apr 29, 2022
Tadalafil has positive effects on neurodevelopment and antioxidant defense system, but there is n... more Tadalafil has positive effects on neurodevelopment and antioxidant defense system, but there is no information for its possible role during gestation on reflexive motor behavior in offspring. So, current study determined effect of prenatal exposure to the Tadalafil on reflexive motor behaviors and antioxidant activity in mice offspring and anti-depressive behaviors in postpartum dams. Forty pregnant female NMRI mice were allocated into four groups. In control group, mice received water while in groups 2-4, female mice orally gavage with Tadalafil(0.4, 0.8 and 1.6 mg/kg) at GD 5, 8, 11, 14 and 17, respectively. Following delivery, pups were selected and reflexive motor behaviors determined using ambulation, hindlimb foot angle, surface righting, hindlimb strength, grip strength, frontlimb suspension and negative geotaxis tests. Also, serum MDA, SOD, GPx and TAS of was determined in offspring. On day 2 postpartum, antidepressant activity of Tadalafil was determined by OFT, rotarod, FST and TST in dams. Based on the findings, maternal exposure to Tadalafil improved ambulation score, hindlimb suspension score, grip strength, front-limb suspension in offspring(P<0.05). Prenatal exposure to Tadalafil decreased surface righting, hind-limb foot angle and negative geotaxis in o offspring (P<0.05). Tadalafil decreased blood MDA and increased SOD and GPx levels in offspring (P<0.05). Tadalafil significantly decreased immobility time in FST and TST and increased number of squares crossed in OFT and spending time on rotarod on postpartum mice(P<0.05). These results suggested parental exposure of Tadalafil has positive effect on reflexive motor and postpartum behaviors.
International Journal of Developmental Neuroscience, Jul 6, 2023
Evidence supports that music can modulate many physiological roles, exerting clear effects on the... more Evidence supports that music can modulate many physiological roles, exerting clear effects on the central nervous system. For this effect to be positive, music should be tuned at a frequency of 432 Hz. This study aims to determine the effects of prenatal exposure to music on reflexive motor behaviors in mice offspring. Six pregnant female NMRI mice (8–10 weeks old) were randomly and equally allocated into two groups. Group 1 as control was placed in a normal housing area (average room noise 35 dB), and Group 2 was exposed to music pitched at 432 Hz for 2 h a day played at constant volume (75/80 dB) during pregnancy. Following delivery, four pups from each pregnant mouse were selected, and reflexive motor behaviors including ambulation, hind‐limb foot angle, surface righting, grip strength, front‐ and hind‐limb suspension, and negative geotaxis were determined. Based on the findings, prenatal exposure to music significantly increased ambulation score, grip strength, and front‐ and hind‐limb suspension compared to the control group (P &lt; 0.05). Also, prenatal exposure to music significantly decreased hind‐limb foot angle, negative geotaxis, and surface righting compared to the control group (P &lt; 0.05). These results suggested that music exposure during pregnancy had a significant positive effect on all tested reflexive motor behaviors in mice offspring.
World Academy of Science, Engineering and Technology, International Journal of Animal and Veterinary Sciences, Jun 19, 2014
International Journal of Peptide Research and Therapeutics, Nov 11, 2014
Your article is protected by copyright and all rights are held exclusively by Springer Science +B... more Your article is protected by copyright and all rights are held exclusively by Springer Science +Business Media New York. This e-offprint is for personal use only and shall not be selfarchived in electronic repositories. If you wish to self-archive your article, please use the accepted manuscript version for posting on your own website. You may further deposit the accepted manuscript version in any repository, provided it is only made publicly available 12 months after official publication or later and provided acknowledgement is given to the original source of publication and a link is inserted to the published article on Springer's website. The link must be accompanied by the following text: "The final publication is available at link.springer.com".
Behavioural Brain Research, Feb 1, 2023
Czech Journal of Animal Science, Aug 31, 2015
Previous studies showed that cyclooxygenase 1 (COX) enzyme has an important role in lipopolysacch... more Previous studies showed that cyclooxygenase 1 (COX) enzyme has an important role in lipopolysaccharide (LPS)-induced hypophagia in mammals but the effect of COX on LPS-induced hypophagia has not been studied in avian species. The current study was designed to investigate the effects of Indomethacin, a non-selective cyclooxygenase inhibitor, Aspirin (irreversible cyclooxygenase inhibitor), Piroxicam (a selective COX-1 inhibitor), and Celecoxib (a selective COX-2 inhibitor) on LPS-induced hypophagia in 3-h fooddeprived (FD 3) cockerels. One hundred and sixty ROSS 308 chickens were randomly divided into 5 experiments and 4 treatment groups (8 replicates in each group of experiments). Guide cannula was surgically implanted into the lateral ventricle of chickens. In Experiment 1, birds received LPS (5, 10, and 20 ng) intracerebroventricularly (ICV). In Experiment 2, chickens were intraperitoneally (i.p.) injected with Indomethacin (5 mg/kg) prior to LPS injection (20 ng; ICV). In Experiment 3, birds were i.p. injected with Aspirin (50 mg/kg) followed by LPS injection (20 ng; ICV). In Experiment 4, chickens were given LPS (20 ng; ICV) after Piroxicam injection (10 mg/kg; i.p.). In Experiment 5, chickens were injected with Celecoxib (10 mg/kg; i.p.) prior to LPS injection (20 ng; ICV). Cumulative feed intake was determined until 8 h post-injection. According to the results, LPS significantly decreased feed intake at 4 and 8 h post injection in birds (P ≤ 0.05). Furthermore, LPS-induced hypophagia was attenuated by pre-injection with Indomethacin, Aspirin, and Celecoxib (P ≤ 0.05). However, Piroxicam had no effect on LPS-induced hypophagia (P ≥ 0.05). These results suggest that presumably COX-2 mediates LPS-induced hypophagia in broilers.
Journal of the Hellenic Veterinary Medical Society, Dec 21, 2017
Aim of the study was to investigate effects of different levels of perlite on intestinal morphome... more Aim of the study was to investigate effects of different levels of perlite on intestinal morphometry in broilers (Ross 308). A hundred and eighty broiler cockerels were randomly allocated into three experimental groups (3 replications and 20 broilers per pen) and fed experimental diets supplemented with different levels of perlite (0%, 2%, 4%). At 21, 28, 35 and 42 days of the study, 2 broilers were randomly selected from each replication, slaughtered and various sections of small intestine (1, 10, 30, 50, 70 and 90% of small intestine length) sampled for morphometry characteristics. Villi height, crypts depth and villus height / crypt depth ratio were measured microscopically. According to the results, a significant difference was observed on small intestine morphology post-perlite supplementation in experimental groups compared to control group. Supplementation of diet with perlite (2%) significantly increased average villi height in various sections of small intestine (1, 70 and 90%) in experimental birds on days 28 and 35 (P < 0.05). In addition, similar findings were observed after addition of perlite (4%) on villi height on day 42 (P < 0.05). Furthermore, on day 28, average villi height and depth of liberkuhn crypts in small intestine (10%) differed significantly in cockerels fed diets containing 2% perlite in comparison to controls (P < 0.05). These results suggest that supplementation of perlite in broilers' diet can improve intestinal morphometry.
Czech Journal of Animal Science, Sep 30, 2015
Opioidergic and serotonergic (5-HTergic) systems have crucial role in central regulation of food ... more Opioidergic and serotonergic (5-HTergic) systems have crucial role in central regulation of food intake. This study was designed to investigate the role of the opioidergic system and the interaction with the 5-HTergic system in opioid-induced feeding behaviour in 3-h food-deprived (FD 3) neonatal layer-type chicks. In total 432 chickens were allocated into 9 experiments, each per 4 treatment groups. In Experiment 1, birds were intracerebroventricularly (ICV) injected with D-Ala2-NMe-Phe4-Glyol5-enkephalin (DAMGO), µ-opioid receptor agonist (125, 250, and 500 pmol). In Experiment 2, chickens were ICV treated with D-Pen 2 , D-Pen 5 enkephalin (D-Pen 2,5 enkephalin, DPDPE), δ-opioid receptor agonist (20, 40, and 80 pmol). In Experiment 3, the effect of ICV injection of U-50488H, κ-opioid receptor agonist (10, 20, and 30 nmol) was investigated in chicks. In Experiment 4, chickens were injected with para-chlorophenylalanine (PCPA), cerebral serotonin depletive (1.5 µg) + DAMGO (125 pmol). In Experiment 5, birds were treated with PCPA (1.5 µg) + DPDPE (40 pmol). In Experiment 6, birds received 1.5 µg PCPA + U-50488H (30 nmol). In Experiments 7-9, birds were injected like in Experiments 4-6, but with SB242084, 5-HT 2 c receptor antagonist (1.5 µg) instead of the PCPA injection. Cumulative food intake was recorded until 3 h post injection. According to the results, the ICV injection of DAMGO significantly decreased whereas that of DPDPE + U-50488H increased food intake (P ≤ 0.05). Co-administration of PCPA + DAMGO significantly decreased hypophagia induced by DAMGO (P ≤ 0.05). PCPA had no effect on DPDPE + U-50488H-induced hyperphagia (P ≥ 0.05). SB242084 significantly attenuated the hypophagic effect of DAMGO (P ≤ 0.05), while SB242084 had no modulatory effect on the food intake induced by DPDPE + U-50488H (P ≥ 0.05). These results suggest that there is an interaction between the opioidergic and 5-HTergic systems mediating the hypophagic effect of µ-opioid receptors via the 5-HT 2 c receptor in neonatal layer-type chicks.
Veterinary Research Communications, Oct 12, 2013
Your article is protected by copyright and all rights are held exclusively by Springer Science +B... more Your article is protected by copyright and all rights are held exclusively by Springer Science +Business Media Dordrecht. This e-offprint is for personal use only and shall not be selfarchived in electronic repositories. If you wish to self-archive your article, please use the accepted manuscript version for posting on your own website. You may further deposit the accepted manuscript version in any repository, provided it is only made publicly available 12 months after official publication or later and provided acknowledgement is given to the original source of publication and a link is inserted to the published article on Springer's website. The link must be accompanied by the following text: "The final publication is available at link.springer.com".
Naunyn-schmiedebergs Archives of Pharmacology, Feb 20, 2023
Crocin is the main bioactive components of the saffron with neuro generative and protective activ... more Crocin is the main bioactive components of the saffron with neuro generative and protective activity, however, its activity in neurodegenerative disease in not fully elicited. So, the aim of the current study was to determine effects of the crocin on re exive motor behavior, depressive and serum antioxidant activities on cuprizone-induced (CPZ) model of multiple sclerosis (MS) mice. 40 male C57BL/6 mice were randomly assigned into 4 groups. Mice in the control group were treated with normal diet. In group 2, CPZ-induced demyelination was done by chew palate containing 0.2% (w/w) CPZ for 5 weeks. In group 3, normal diet was provided and mice orally received crocin (100 mg/kg) 3 times per week for 5 weeks. In group 4, mice feed CPZ containing diet and orally received crocin (100 mg/kg) three times per for 5 weeks. At the end of the study, re exive motor behavior and depressive tests were done. Also, serum and brain tissue antioxidant activity was determined. According to the data, crocin had positive effects on hind-limb foot angle, hind-and front-limb suspension, surface righting, grip strength and negative geotaxis while CPZ had adverse effect compare to control group (P < 0.05). Co-administration of the CPZ + crocin signi cantly decreased adverse effect of the CPZ on the re exive motor behavior tests (P < 0.05). CPZ signi cantly increased immobility time in the forced swimming test (FST), tail suspension test (TST) and crocin diminished it (P < 0.05). Co-administration of the CPZ + crocin signi cantly decreased adverse effect of the CPZ on immobility time (P < 0.05). CPZ decreased number of cross in open eld test (OFT) and spending time on rotarod and CPZ + crocin signi cantly lessened adverse effect of the CPZ (P < 0.05). CPZ signi cantly increased malondialdehyde (MDA) and decreased glutathione peroxidase (GPx), superoxide dismutase (SOD) and total antioxidant status (TAS) and these effects reversed by crocin in brain tissue and serum (P < 0.05). Co-administration of the CPZ + crocin signi cantly improved adverse effect of the CPZ on serum and brain tissue antioxidants (P < 0.05). These results suggested crocin has protective effect against on CPZ-induced MS in mice.
International Journal of Developmental Neuroscience, Feb 23, 2023
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Papers by Shahin Hassanpour