Papers by Luciana Lasry Benchimol Reis
Frontiers in plant science, 2015
Genetic resistance of common bean (Phaseolus vulgaris L.) against angular leaf spot (ALS), caused... more Genetic resistance of common bean (Phaseolus vulgaris L.) against angular leaf spot (ALS), caused by the fungus Pseudocercospora griseola, is conferred by quantitative trait loci (QTL). In this study, we determined the gene content of the major QTL ALS10.1 located at the end of chromosome Pv10, and identified those that are responsive to ALS infection in resistant (CAL 143) and susceptible (IAC-UNA) genotypes. Based on the current version of the common bean reference genome, the ALS10.1 core region contains 323 genes. Gene Ontology (GO) analysis of these coding sequences revealed the presence of genes involved in signal perception and transduction, programmed cell death (PCD), and defense responses. Two putative R gene clusters were found at ALS10.1 containing evolutionary related coding sequences. Among them, the Phvul.010G025700 was consistently up-regulated in the infected IAC-UNA suggesting its contribution to plant susceptibility to the fungus. We identified six other genes tha...
Additional file 2: Table S1. Cultivar name, grain size (mm), commercial classification, instituti... more Additional file 2: Table S1. Cultivar name, grain size (mm), commercial classification, institution of origin, genealogy, adjusted phenotypic mean (BLUE) for lightness at harvest and tolerance to PHD, and genotypic matrix for the 138 carioca common bean genotypes of the carioca diversity panel (CDP).
BMC Plant Biology, 2021
Background Common bean (Phaseolus vulgaris L.) is a legume whose grain can be stored for months, ... more Background Common bean (Phaseolus vulgaris L.) is a legume whose grain can be stored for months, a common practice among Brazilian growers. Over time, seed coats become darker and harder to cook, traits that are undesirable to consumers, who associate darker-colored beans with greater age. Like commercial pinto and cranberry bean varieties, carioca beans that have darker seeds at harvest time and after storage are subject to decreased market values. Results The goal of our study was to identify the genetic control associated with lightness of seed coat color at harvest (HL) and with tolerance to post-harvest seed coat darkening (PHD) by a genome-wide association study. For that purpose, a carioca diversity panel previously validated for association mapping studies was used with 138 genotypes and 1,516 high-quality SNPs. The panel was evaluated in two environments using a colorimeter and the CIELAB scale. Shelf storage for 30 days had the most expressive results and the L* (luminosit...
Tropical Plant Pathology, 2022
Additional file 4: Table 3S. Bayesian Information Criterion (BIC) test according to Schwarz [63],... more Additional file 4: Table 3S. Bayesian Information Criterion (BIC) test according to Schwarz [63], for all traits evaluated using the first five components of the principal component analysis.
Additional file 1: Figure S1. (a) Principal component analysis based on the 1, 516 SNPs for the c... more Additional file 1: Figure S1. (a) Principal component analysis based on the 1, 516 SNPs for the carioca diversity panel (n = 138); (b) Kinship heatmap plot showing the population relationship, estimated according to VanRaden [68]. (c) Linkage disequilibrium (LD) decay determined by the LD measurements (r2) against the distance between SNPs (Mb) for the 11 chromosomes (Pv) adjusted according to the model proposed by Hill and Weir [69] controlled for relatedness.
Common bean has a high sensitivity to drought stress, particularly during reproductive developmen... more Common bean has a high sensitivity to drought stress, particularly during reproductive development which reduces its yield. In this study we aimed to: (i) evaluate differences in shoot and root response of a mapping population of 107 recombinant inbred lines (RILs) of SEA 5 × AND 277 cross under drought stress, and (ii) identify the QTLs associated with improved plant performance under water stress treatment. Phenotyping was performed under greenhouse conditions where the plants were grown using rhizotrons under well-watered and water-stress conditions. Four QTLs were identified that are related to improved performance under water stress and three of them were related specifically to roots and these are located on chromosomes Pv1 and Pv6. Root superficial area trait explained 32.6% of the variance and may contribute to greater water uptake and improved adaptation of common bean under water stress conditions.
PLOS ONE, 2021
Brazil is the largest consumer of dry edible beans (Phaseolus vulgaris L.) in the world, 70% of c... more Brazil is the largest consumer of dry edible beans (Phaseolus vulgaris L.) in the world, 70% of consumption is of the carioca variety. Although the variety has high yield, it is susceptible to several diseases, among them, anthracnose (ANT) can lead to losses of up to 100% of production. The most effective strategy to overcome ANT, a disease caused by the fungus Colletotrichum lindemuthianum, is the development of resistant cultivars. For that reason, the selection of carioca genotypes resistant to multiple ANT races and the identification of loci/markers associated with genetic resistance are extremely important for the genetic breeding process. Using a carioca diversity panel (CDP) with 125 genotypes and genotyped by BeadChip BARCBean6K_3 and a carioca segregating population AM (AND-277 × IAC-Milênio) genotyped by sequencing (GBS). Multiple interval mapping (MIM) and genome-wide association studies (GWAS) were used as mapping tools for the resistance genes to the major ANT physiol...
Frontiers in Plant Science, 2021
Angular leaf spot (ALS) is a disease that causes major yield losses in the common bean crop. Stud... more Angular leaf spot (ALS) is a disease that causes major yield losses in the common bean crop. Studies based on different isolates and populations have already been carried out to elucidate the genetic mechanisms of resistance to ALS. However, understanding of the interaction of this resistance with the reproductive stages of common bean is lacking. The aim of the present study was to identify ALS resistancelociat different plant growth stages (PGS) by association and linkage mapping approaches. An BC2F3inter-gene pool cross population (AND 277 × IAC-Milênio – AM population) profiled with 1,091 SNPs from genotyping by sequencing (GBS) was used for linkage mapping, and a carioca diversity panel (CDP) genotyped by 5,398 SNPs from BeadChip assay technology was used for association mapping. Both populations were evaluated for ALS resistance at the V2 and V3 PGSs (controlled conditions) and R8 PGS (field conditions). Different QTL (quantitative traitloci) were detected for the three PGSs a...
Genes, 2021
Fusarium wilt (Fusarium oxysporum f. sp. phaseoli, Fop) is one of the main fungal soil diseases i... more Fusarium wilt (Fusarium oxysporum f. sp. phaseoli, Fop) is one of the main fungal soil diseases in common bean. The aim of the present study was to identify genomic regions associated with Fop resistance through genome-wide association studies (GWAS) in a Mesoamerican Diversity Panel (MDP) and to identify potential common bean sources of Fop’s resistance. The MDP was genotyped with BARCBean6K_3BeadChip and evaluated for Fop resistance with two different monosporic strains using the root-dip method. Disease severity rating (DSR) and the area under the disease progress curve (AUDPC), at 21 days after inoculation (DAI), were used for GWAS performed with FarmCPU model. The p-value of each SNP was determined by resampling method and Bonferroni test. For UFV01 strain, two significant single nucleotide polymorphisms (SNPs) were mapped on the Pv05 and Pv11 for AUDPC, and the same SNP (ss715648096) on Pv11 was associated with AUDPC and DSR. Another SNP, mapped on Pv03, showed significance fo...
Tropical Plant Pathology, 2020
Common bean (Phaseolus vulgaris L.) is one of the most widely grown legumes in the world. Althoug... more Common bean (Phaseolus vulgaris L.) is one of the most widely grown legumes in the world. Although the crop has high yield potential, average yields in Brazil are low due to several diseases. Angular leaf spot (ALS), caused by Pseudocercospora griseola, is among the most important diseases. A set of 81 accessions from the Instituto Agronômico (IAC, Campinas, SP) germplasm bank were evaluated for ALS resistance and genotyped by 12 microsatellites previously associated with ALS QTL resistance. Allele frequencies, number of alleles per locus, expected heterozygosity (He), and Shannon's Information Index (I) were calculated. The average Ho was 0.12, and the He was 0.54. The STRUCTURE analysis and UPGMA clustering based on Nei's genetic distance indicated a moderate degree of genetic diversity, with 4 and 5 main groups, respectively. Evaluation of the severity of ALS showed that 17% of the accessions had resistance. Cultivars were recommended for breeding crosses aimed at gaining in genetic diversity and resistance to ALS.
Journal of Applied Genetics, 2021
Brazil is among the largest producers and consumers of common bean (Phaseolus vulgaris L.) and ca... more Brazil is among the largest producers and consumers of common bean (Phaseolus vulgaris L.) and can be considered a secondary center of diversity for the species. The aim of this study was to estimate the genetic diversity, population structure, and relationships among 288 common bean accessions in an American Diversity Panel (ADP) genotyped with 4,042 high-quality single nucleotide polymorphisms (SNPs). The results showed inter-gene pool hybridization (hybrids) between the two main gene pools (i.e., Mesoamerican and Andean), based on principal component analysis (PCA), discriminant analysis of principal components (DAPC), and STRUCTURE analysis. The genetic diversity parameters showed that the Mesoamerican group has higher values of diversity and allelic richness in comparison with the Andean group. Considering the optimal clusters (K), clustering was performed according to the type of grain (i.e., market group), the institution of origin, the period of release, and agronomic traits. A new subset was selected and named the Mesoamerican Diversity Panel (MDP), with 205 Mesoamerican accessions. Analysis of molecular variance (AMOVA) showed low genetic variance between the two panels (i.e., ADP and MDP) with the highest percentage of the limited variance among accessions in each group. The ADP showed occurrence of high genetic differentiation between populations (i.e., Mesoamerican and Andean) and introgression between gene pools in hybrids based on a set of diagnostic SNPs. The MDP showed better linkage disequilibrium (LD) decay. The availability of genetic variation from inter-gene pool hybridizations presents a potential opportunity for breeders towards the development of superior common bean cultivars.
Molecular Breeding, 2014
Your article is protected by copyright and all rights are held exclusively by Springer Science +B... more Your article is protected by copyright and all rights are held exclusively by Springer Science +Business Media Dordrecht. This e-offprint is for personal use only and shall not be selfarchived in electronic repositories. If you wish to self-archive your article, please use the accepted manuscript version for posting on your own website. You may further deposit the accepted manuscript version in any repository, provided it is only made publicly available 12 months after official publication or later and provided acknowledgement is given to the original source of publication and a link is inserted to the published article on Springer's website. The link must be accompanied by the following text: "The final publication is available at link.springer.com".
Theoretical and Applied Genetics, 2013
Pesquisa Agropecuária Brasileira, 2008
The objective of this work was to develop new microsatellite markers in common bean. Ninety nine ... more The objective of this work was to develop new microsatellite markers in common bean. Ninety nine new microsatelitte loci were developed from a microsatellite enriched library for (CT)8 and (GT)8 motifs, from CAL-143 line. The majority of microsatellite sequences (51%) was related to cellular metabolism. The remaining sequences were associated to transcription functions. Only 17.2% of the sequences presented some level of similarity with other plant species genes.
Root-knot nematodes (RKN), particularly Meloidogyne incognita, are among the most damaging and pr... more Root-knot nematodes (RKN), particularly Meloidogyne incognita, are among the most damaging and prevalent agricultural pathogens due to their ability to infect roots of almost all crop species, including common bean. The best strategy for their control is through the use of resistant cultivars. However, laborious phenotyping procedures make it difficult to assess nematode resistance in breeding programs. For common bean, this task is especially challenging since little has been done to discover resistance genes or find markers to assist selection. In this study, we performed genome-wide association studies and QTL mapping to explore the genetic architecture and genomic regions underlying the resistance to M. incognita and to identify candidate resistance genes. Phenotypic data were collected by a high-throughput assay, and the number of egg masses and root-galling index were evaluated 30 days after inoculation. Complex genetic architecture and independent genomic regions were associa...
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Papers by Luciana Lasry Benchimol Reis