Papers by Antonios Kanaras
This dataset supports the publication: "An Investigation Into the Resistance of Spherical Nu... more This dataset supports the publication: "An Investigation Into the Resistance of Spherical Nucleic Acids Against DNA Enzymatic Degradation" Maria-Eleni Kyriazi, Afaf H. El-Sagheer, Igor L. Medintz, Tom Brown, Antonios G. Kanaras* The paper has been accepted for publication in the ACS Journal, Bioconjugate Chemistry
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
To support article "A Method for the Growth of Uniform Silica Shells on Different Size and M... more To support article "A Method for the Growth of Uniform Silica Shells on Different Size and Morphology Upconversion Nanoparticles" in Nanoscale Advances
Additional file 1. Additional tables and figures.
Colloidal Nanoparticles for Biomedical Applications XIV, Mar 7, 2019
In this paper we present an optical characterization of nanoparticle assemblies containing plasmo... more In this paper we present an optical characterization of nanoparticle assemblies containing plasmonic gold nanoparticles and fluorescent upconversion nanoparticles in different ratios. Both the two-photon luminescence derived from gold nanoparticles and the fluorescence originated from upconversion nanoparticles are detected. The optical signatures reflect the composition of the assemblies.
ABSTRACTHuman bone marrow (BM) derived stromal cells contain a population of skeletal stem cells ... more ABSTRACTHuman bone marrow (BM) derived stromal cells contain a population of skeletal stem cells (SSCs), with the capacity to differentiate along the osteogenic, adipogenic and chondrogenic lineages enabling their application to clinical therapies. However, current methods, to isolate and enrich SSCs from human tissues remain, at best, challenging in the absence of a specific SSC marker. Unfortunately, none of the current proposed markers, alone, can isolate a homogenous cell population with the ability to form bone, cartilage, and adipose tissue in humans. Here, we have designed DNA-gold nanoparticles able to identify and sort SSCs displaying specific mRNA signatures. The current approach demonstrates the significant enrichment attained in the isolation of SSCs, with potential therein to enhance our understanding of bone cell biology and translational applications.TABLE OF CONTENTS
International journal of nanomedicine, 2016
In this work, peptides designed to selectively interact with cellular receptors involved in the r... more In this work, peptides designed to selectively interact with cellular receptors involved in the regulation of angiogenesis were anchored to oligo-ethylene glycol-capped gold nanoparticles (AuNPs) and used to evaluate the modulation of vascular development using an ex ovo chick chorioallantoic membrane assay. These nanoparticles alter the balance between naturally secreted pro- and antiangiogenic factors, under various biological conditions, without causing toxicity. Exposure of chorioallantoic membranes to AuNP-peptide activators of angiogenesis accelerated the formation of new arterioles when compared to scrambled peptide-coated nanoparticles. On the other hand, antiangiogenic AuNP-peptide conjugates were able to selectively inhibit angiogenesis in vivo. We demonstrated that AuNP vectorization is crucial for enhancing the effect of active peptides. Our data showed for the first time the effective control of activation or inhibition of blood vessel formation in chick embryo via AuNP...
Physical chemistry chemical physics : PCCP, Jan 18, 2016
In the present work, a combination of imaging, spectroscopic and computational methods shows that... more In the present work, a combination of imaging, spectroscopic and computational methods shows that 1-dodecanethiol undergoes S-deprotonation to form 1-dodecanethiolate on the surface of palladium nanoparticles, which then self-assembles into a structure that shows a high degree of order. The alkyl chain is largely in the all-trans conformation, which occurs despite the small size of the nanoparticle, (mean diameter = 3.9 nm). Inelastic neutron scattering spectroscopy is readily able to characterise organic surface layers on nanoparticles; the nature of the material is irrelevant: whether the nanoparticle core is an oxide, a metal or a semiconductor makes no difference. Comparison to DFT calculations allows insights into the nature and conformation of the adsorbed layer.
J. Mater. Chem. C, 2015
Programmed and covalent nanoparticle attachment onto graphene oxide using DNA click ligation chem... more Programmed and covalent nanoparticle attachment onto graphene oxide using DNA click ligation chemistry.
Applied Physics A, 2014
We present a chemical process for the decoration of self-assembled two-dimensional peptide fibril... more We present a chemical process for the decoration of self-assembled two-dimensional peptide fibrils with two different sizes of CdSe@ZnS core-shell quantum dots (Qdots) capped with trioctylphosphine oxide molecules. The attachment of the semiconducting nanoparticles to the fibrils is directed via disulfide bond between the quantum dot and cysteine aminoacids that are deliberately impeded in the peptide structures. A significant red shift in the emission spectra of the quantum dots is observed and attributed to the synergistic interaction between adjacent nanoparticles arranged on peptide film templates extending over hundreds of nanometers.
Small, 2007
Financial support by the European Union (BIOSCOPE) is gratefully acknowledged. Research was parti... more Financial support by the European Union (BIOSCOPE) is gratefully acknowledged. Research was partially supported by the University of Liverpool. Z.W. acknowledges support from the BBSRCfunded Liverpool Centre for Bioarray Innovation.
Physical Chemistry Chemical Physics, 2013
Hyperspectral microscopy is a versatile method for simultaneous spatial and spectroscopic charact... more Hyperspectral microscopy is a versatile method for simultaneous spatial and spectroscopic characterization of nonfluorescent samples. Here we present a hyperspectral darkfield imaging system for spectral imaging of single nanoparticles over an area of 150 × 150 µm 2 and at illumination intensities compatible with live cell imaging. The capabilities of the system are demonstrated using correlated transmission electron microscopy and single-particle optical studies of colloidal hollow gold nanoparticles. The potential of the system for characterizing the interactions between nanoparticles and cells has also been demonstrated. In this case, the spectral information proves a useful improvement to standard darkfield imaging as it enables differentiation between light scattered from nanoparticles and light scattered from other sources in the cellular environment. The combination of low illumination power and fast integration times makes the system highly suitable for nanoparticle tracking and spectroscopy in live-cell experiments.
Nanoscale, 2012
We present the exocytosis profile of two types of peptide-coated nanoparticles, which have simila... more We present the exocytosis profile of two types of peptide-coated nanoparticles, which have similar charge and size but different functionality. While one kind of particles appears to progressively exocytose, the other one has a more complex profile, suggesting that some of the particles are re-uptaken by the cells. Both types of particles retain their colloidal stability after exocytosis.
ACS Nano, 2013
We demonstrate the deliberate activation or inhibition of in vitro angiogenesis using functional ... more We demonstrate the deliberate activation or inhibition of in vitro angiogenesis using functional peptide coated gold nanoparticles. The peptides, anchored to oligo-ethylene glycol capped gold nanospheres, were designed to selectively interact with cell receptors responsible for activation or inhibition of angiogenesis. The functional particles are shown to influence significantly the extent and morphology of vascular structures, without causing toxicity. Mechanistic studies show that the nanoparticles have the ability to alter the balance between naturally secreted pro-and anti-angiogenic factors, under various biological conditions. Nanoparticle-induced control over angiogenesis opens up new directions in targeted drug delivery and therapy.
This dataset supports the publication "A DNA Sensor Based on Upconversion Nanoparticles and ... more This dataset supports the publication "A DNA Sensor Based on Upconversion Nanoparticles and 2D Dichalcogenide Materials" Konstantina Alexaki, Davide Giust, Maria-Eleni Kyriazi, Afaf H. El-Sagheer, Tom Brown, Otto L. Muskens and Antonios G. KanarasFrontiers of Chemical Science and Engineering. This dataset is released under a CC BY 4.0 licence.
Raw data and images for the article Vilela, Patrick, El-Sagheer, Afaf, Millar, Timothy M., Brown,... more Raw data and images for the article Vilela, Patrick, El-Sagheer, Afaf, Millar, Timothy M., Brown, Tom, Muskens, Otto and Kanaras, Antonios (2016) Graphene oxide-upconversion nanoparticle based optical sensors for targeted detection of mRNA biomarkers present in Alzheimer's disease and prostate cancer. ACS Sensors (doi:10.1021/acssensors.6b00651)
Dataset supports: Moros, Maria et al (2018). DNA – Coated Gold Nanoparticles for the Detection of... more Dataset supports: Moros, Maria et al (2018). DNA – Coated Gold Nanoparticles for the Detection of mRNA in Live Hydra Vulgaris Animals. ACS Applied Materials and Interfaces
Dataset supports: Giust, D. et al (2018). Graphene Oxide–Upconversion Nanoparticle Based Portable... more Dataset supports: Giust, D. et al (2018). Graphene Oxide–Upconversion Nanoparticle Based Portable Sensors for Assessing Nutritional Deficiencies in Crops. ACS Nano.
Colloidal Nanoparticles for Biomedical Applications XIV
Recently DNAcoated gold nanoparticles have emerged as ideal tools for the detection of mRNA in ce... more Recently DNAcoated gold nanoparticles have emerged as ideal tools for the detection of mRNA in cells using dye modified oligonucleotides. However, the tracking of the gold core has been hindered by the small size of the particle core. In this work we utilize a home built set up and 43 nm DNAcoated spherical gold nanoparticles for the simultaneous imaging of mRNA detection using fluorescence microscopy and the gold nanoparticle core using two photon photoluminescence (TPPL).
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Papers by Antonios Kanaras